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Potent allelopathy and non-PSTs, non-spirolides toxicity of the dinoflagellate Alexandrium leei to phytoplankton, finfish and zooplankton observed from laboratory bioassays

Shang L, Xu Y, Leaw CP, Lim PT, Wang J, Chen J, et al.

Sci Total Environ, 2021 Aug 01;780:146484.
PMID: 33774286 DOI: 10.1016/j.scitotenv.2021.146484

The dinoflagellate genus Alexandrium has been well known for causing paralytic shellfish poisoning (PSP) worldwide. Several non-PSP toxin-producing species, however, have shown to exhibit fish-killing toxicity. Here, we report the allelopathic activity of Alexandrium leei from Malaysia to other algal species, and its toxicity to finfish and zooplankton, via laboratory bioassays. Thirteen microalgal species that co-cultured with Al. leei revealed large variability in the allelopathic effects of Al. leei on the test algae, with the growth inhibition rates ranging from 0 to 100%. The negative allelopathic effects of Al. leei on microalgae included loss of flagella and thus the motility, damages of chain structure, deformation in cell morphology, and eventually cell lysis. The finfish experienced 100% mortality within 24 h exposed to the live culture (2000-6710 cells·mL-1), while the rotifer and brine shrimp exhibited 96-100% and 90-100% mortalities within 48 h when exposed to 500-6000 cells·mL-1 of Al. leei. The mortality of the test animals depended on the Al. leei cell density exposed, leading to a linear relationship between mortality and cell density for the finfish, and a logarithmic relationship for the two zooplankters. When exposed to the treatments using Al. leei whole live culture, cell-free culture medium, extract of algal cells in the f/2-Si medium, extract of methanol, and the re-suspended freeze-and-thaw algal cells, the test organisms (Ak. sanguinea and rotifers) all died at the cell density of 8100 cells·mL-1 within 24 h. Toxin analyses by HILIC-ESI-TOF/MS and LC-ESI-MS/MS demonstrated that Al. leei did not produce PSP-toxins and 13-desmethyl spirolide C. Overall, our findings demonstrated potent allelopathy and toxicity of Al. leei, which do not only pose threats to the aquaculture industry, fisheries, and marine ecosystems but may also play a part role in the population dynamics and bloom formation of this species.

Matched MeSH terms: Laboratories
Fulltext Impact of diabetes mellitus on clinico-laboratory characteristics and in-hospital clinical outcomes among patients with myocardial infarction

Khalid SH, Liaqat I, Mallhi TH, Khan AH, Ahmad J, Khan YH

J Pak Med Assoc, 2020 Dec;70(12(B)):2376-2382.
PMID: 33475547 DOI: 10.47391/JPMA.370

OBJECTIVE: Diabetes mellitus (DM) along with myocardial infarction (MI) carries increased burden on patients in terms of morbidity, mortality and cost. Current study was aimed to investigate the impact of DM on clinico-laboratory characteristics on in-hospital treatment outcomes among MI patients.o compare the outcome of mesh hernioplasty performed under local anaesthesia in relatively young and older patients regarding wound complications and urinary retention.
METHODS: All MI patients admitted to the emergency department of Faisalabad Institute of Cardiology from April, 2016 to March, 2017 were recruited into the study. The clinico-laboratory profile and in-hospital outcomes of patients with and without DM were compared using chi-squared test or student t-test, where appropriate.
RESULTS: A total 4063 patients (Mean age: 55.86 ± 12.37years) with male preponderance were included into the study. STEMI was most prevalent (n = 2723, 67%) type of MI among study participants. DM was present in substantial number of cases (n = 3688, 90.8%). Patients with DM presented with increased BMI, higher blood pressure, elevated levels of cholesterol, serum creatinine, and blood urea nitrogen, when compared to the patients without DM (p<0.05). Out of 560 patients who were followed up, cardiogenic shock was frequent (n = 293, 52.3%) adverse outcome followed by heart failure (n = 114, 20.4%), atrial fibrillation (n = 78, 13.9%) and stroke (n = 75, 13.4 %). Moreover, in-hospital adverse outcomes were more prevalent among MI patients with DM than those without DM.
CONCLUSIONS: MI patients with DM present with varying clinico laboratory characteristics as well as experience higher prevalence of adverse cardiovascular events as compared to patients without DM. These patients require individual management strategy on very first day of admission.

Matched MeSH terms: Laboratories
A new species of cockroach, Periplaneta gajajimana sp. nov., collected in Gajajima, Kagoshima Prefecture, Japan

Komatsu N, Lio H, Ooi HK

Trop Biomed, 2021 Jun 01;38(2):48-52.
PMID: 33973572 DOI: 10.47665/tb.38.2.036

We described a new species of cockroach, Periplaneta gajajimana sp. nov., which was collected in Gajajima, Kagoshima-gun Toshimamura, Kagoshima Prefecture, Japan, on November 2012. The new species is characterized by its reddish brown to blackish brown body, smooth surface pronotum, well developed compound eyes, dark brown head apex, dark reddish brown front face and small white ocelli connected to the antennal sockets. In male, the tegmen tip reach the abdomen end or are slightly shorter, while in the female, it does not reach the abdominal end and exposes the abdomen beyond the 7th abdominal plate. We confirmed the validity of this new species by breeding the specimens in our laboratory to demonstrate that the features of the progeny were maintained for several generations. For comparison and easy identification of this new species, the key to species identification of the genus Periplaneta that had been reported in Japan to date are also presented.

Matched MeSH terms: Laboratories
Fulltext Delivery time of orthodontic appliances from government dental laboratories in Selangor

Loke, S.T., Tan, S.Y.

Malaysian Dental Journal, 2011;32(1):5-11.
MyJurnal

Compliance to delivery of orthodontic appliances within specified times and factors which influenced compliance by dental technicians in Selangor was evaluated. This is a prospective 8-month study of 18 trainee/ trained technicians from 4 main government dental laboratories in Kajang, Klang, Tanjong Karang and Shah Alam. Delivery times specified by orthodontists were 1 day for plastic retainer, 3 days acrylic retainer, 5 days active plates (URA), 10 days functional appliances (FA), 10 days transpalatal arch (TPA) and 10 days for quadhelix. Punctual delivery was recorded as ‘compliant’. Compliance was compared between appliances, clinics, technicians, orthodontists and seniority of technicians. The sample comprised appliances from 365 patients; 38 (10.4%) Tanjong.Karang, 114 (31.2%) Kajang, 191 (52.3%) Klang and 22 (6.0%) Shah Alam. The majority of appliances were retainers (66.3%), followed by URA (13.4%), functionals (9.3%), TPA (9.0%) and quadhelix (1.9%). Mean compliance for all appliances in Selangor was 55%. Plastic retainers had highest compliance (77.8%), followed by acrylic retainers (59.9%), quadhelix (57.1%), FA (47.1%), TPA (45.5%) and URA (24.5%). Senior technicians (>3 years in service) were more compliant than juniors (

Matched MeSH terms: Laboratories, Dental
Fulltext GIS based assessment on landslide hazard zonation: case study of Cameron Highlands – Gua Musang road Kelantan, Malaysia

Mohamed M. GahGah, Juhari Mat Akhir, Abdul Ghani M. Rafek, Ibrahim Abdullah

Sains Malaysiana, 2009;38(6):827-833.

The aim of this study is to investigate the factors that cause landslides in the area along the new road between Cameron Highlands and Gua Musang. Landslide factors such as lineaments have been extracted from remote sensing data (Landsat TM image) using ERDAS software. A soil map has been produced using field work and laboratory analysis, and the lithology, roads, drainage pattern and rainfall have been digitized using ILWIS software together with the slope angle and elevation from the Digital Elevation Model (DEM). All these parameters, which are vital for landslide hazard assessment, have been integrated into the geographical information system (GIS) for further data processing. Weightage for these landslide relevant factors related to their influence in landslide occurrence using the heuristic method has been carried out. The results from this combination through a modified ‘index overlay with multi class maps’ model was used to produce a landslide hazard zonation map. Five classes of potential landslide hazard have been derived as the following: very low hazard zone 17.27%, low hazard zone 39.35%, medium hazard zone 25.1%, high hazard zone 15.35% and very high hazard zone 2.93%. The results from this work have been checked through the landslide inventory using available aerial photos interpretation and field work, and show that the slope and elevation have the most direct affect on landslide occurrence.

Matched MeSH terms: Laboratories
Fulltext Hemolyzed Specimens: Major Challenge for Identifying and Rejecting Specimens in Clinical Laboratories

Wan Azman WN, Omar J, Koon TS, Tuan Ismail TS

Oman Med J, 2019 Mar;34(2):94-98.
PMID: 30918601 DOI: 10.5001/omj.2019.19

Pre-analytical quality in clinical chemistry testing is as important as analytical and post-analytical quality. The most prevalent pre-analytical interference and a major source of error producing unreliable laboratory test results is hemolysis of blood samples. In vitro hemolysis may be due to the blood withdrawal technique or sample handling whereas in vivo hemolysis can originate from acquired, hereditary, or iatrogenic conditions and is not technique dependent. Interpreting in vivo or in vitro hemolysis requires clinicians to supply reliable clinical history and findings. Even then, to reject or release the result with interpretation is still under debate. Thus, hemolyzed specimens are a serious pre-analytical problem calling for well-designed and strictly implemented laboratory guidelines. The aim of this non-systematic review (addressed to healthcare professionals) was to highlight the challenges in identifying and rejecting hemolysis specimens.

Matched MeSH terms: Laboratories
Prevention of Group A streptococcal infection in Malaysia

Jamal F

Family Practitioner, 1988;11(1):73-74.

Group A streptococcus is an important cause of pharyngitis, skin infection and or its non-suppurative sequelae, i.e. rheumatic fever and post-streptococcal glomerulonephritis. The National Streptococcus Reference Laboratory was established in 1982 to collect data on various aspects of this infection.

Matched MeSH terms: Laboratories
Fulltext Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID)

Mad' Atari MFB, Folta KM

BMC Res Notes, 2019 Mar 15;12(1):144.
PMID: 30876440 DOI: 10.1186/s13104-019-4117-3

OBJECTIVE: The treatment of plant tissue with Agrobacterium tumefaciens is often a critical first step to both stable and transient plant transformation. In both applications bacterial suspensions are oftentimes physically introduced into plant tissues using hand-driven pressure from a needleless syringe. While effective, this approach has several drawbacks that limit reproducibility. Pressure must be provided with the syringe perfectly perpendicular to the tissue surface. The researcher must also attempt to provide even and consistent pressure, both within and between experimental replicates. These factors mean that the procedures do not always translate well between research groups or biological replicates.
RESULTS: We have devised a method to introduce Agrobacterium suspensions into plant leaves with greater reproducibility. Using a decommissioned dissecting microscope as an armature, a syringe body with the bacterial suspension is mounted to the nosepiece. Gentle, even pressure is applied by rotating the focus knob. The treatment force is measured using a basic kitchen scale. The development of the Standardized Pressure Agrobacterium Infiltration Device (SPAID) provides a means to deliver consistent amounts of bacterial suspensions into plant tissues with the goal of increasing reproducibility between replicates and laboratories.

Matched MeSH terms: Laboratories
Immigrant Health Study. 10. Pilot data - what does it tell? [Abstract]

Atiya AS, Anuar Zaini MZ, Khairul Anuar A

JUMMEC, 1998;3:64-65.

A pilot study on 250 foreign workers was undertaken during a 7-month period beginning December 1996. The sample of subjects mainly males (88.8%) was selected on a non-probability basis from two sources i.e. from University of Malaya (72.8%) and PEREMBA group (27.2%). The study was a clinic-based and a face-to-face interview was carried out to elicit information on social, demographic, environmental, medical and recent illness using a structured questionnaire. Physical examinations were also performed on the same day of the interview. Subjects were also required to give their stool, venous blood, and urine specimens for microbiological, parasitological and clinical laboratory investigations. Chest X-Rays was done on ail subjects. The other investigators had already reported findings on the various specific areas of the study. In this part of the report attempt was made to relate the infectious diseases to some of the socio-environtnental variables on the 112 Indonesian, 133 Bangladeshi workers. Some aspects of health seeking behaviour of these foreign workers were also presented. Most of the Indonesian workers (84%) were from Jawa Timua and Jambi, Sumatra, while majority of the Bangladeshis (67.7%) were from two neighboring administrative districts of Dhaka and Chittagong. Majorities of the Indonesians (50.0%) were working in service industry, while 53.5% Bangladeshis were in the manufacturing. One-fifth of the workers lived in squatter areas, and nearly half of them were working for the service industry. About 70% of the workers had at least one infection. The proportion was slightly higher among the Indonesians (72.3%) compared to the Bangladeshis (67.7%). It is of interest to point out that 40.0% had multiple infections. Thirteen had five or more infections (details for the two of the 13 cases are presented as case studies). However, the findings did not indicate any association between sanitation and infections. Risk for transmission was developed based on the number of infections in the person. The Indonesian workers carried a higher risk of transmitting the diseases (33.9%) compared to 19.5% among the Bangladeshi workers. Those working in the construction industry were at a higher risk of transmitting the diseases compared to other industries. Slightly more than half of the workers experienced some form of minor illness or injury during the two-week period preceding the interview. Majority sought private care (43.1%), while 42.3% either self-medicate or did nothing al all. Nearly two-thirds paid out of their own pocket. Among the employers, construction sector made negligible contribution (2.9%) to the payment. It is interesting to find that 41.0% of the workers took some form of health suppletnents, and majority (48.4%) got it from the phartnacy or traditional sources. Nearly all (88.5%) paid on their own for the health supplements. The findings from this pilot project need to be interpreted with solne caution. However, it appears that the foreign workers carry sizeable anlount of health problems. If these are not addressed quickly it may eudanger the health of a nation, while we readily acknowledge their contribution towards our national development.

Matched MeSH terms: Laboratories
Fulltext Guideline on medical laboratory safety in health clinic. [Buku Panduan Keselamatan Makmal Perubatan Klinik Kesihatan.]

ISBN: 978-967-5322-02-0

Citation: Buku Panduan Keselamatan Makmal Perubatan Klinik Kesihatan. Putrajaya: Bahagian Pembangunan Kesihatan Keluarga, Kementerian Kesihatan Malaysia; 2009

Translation:
Guideline on medical laboratory safety in health clinic. Putrajaya: Family Health Development Division, Ministry of Health, Malaysia; 2009

Matched MeSH terms: Laboratories
Pilot test of a fermentation tank for producing coal methane through anaerobic fermentation

Daping Xia, Huaiwen Zhang, Xile Liu, Chaoyong Fu, Xianbo Su

Sains Malaysiana, 2017;46:2083-2089.

The development and utilization of clean energy has long been a focus of research. In the coal bed methane field, most coal bed biogenic methane experiments are small static sample tests in which the initial conditions are set and the process cannot be batch-fed elements and microbial strains, and the gas cannot be collected in batches. Although significant results have been achieved in the coal-to-biogenic methane conversion in China, findings are restricted to the laboratory scale. No successful commercialization of coal bed biogenic methane production has been achieved yet. This study used a large-capacity fermentation tank (5 L) to conduct biogenic methane experiments. Results were compared to those from the traditional laboratory test. The gas production rate and gas concentration were higher when the 250 mL methane test volume was increased to a 5 L fermentation volume, increasing by 20.9% and 2.3%, respectively. The inhibition effect of the liquid phase products was reduced in the large fermentation tank, and the microbial activity was extended by batch feeding trace elements (iron and nickel) and methane strains and by semi-continuous collection of the gas. However, the gas conversion rate can be increased by retaining the H2 and CO2 in the intermediate gas products in the fermentation tank. The gas production rate was increased from 17.9 to 24.6 mL/g, increasing by 37.4%. The simulation pilot test can lay a foundation for the transition from a coal bed biogenic methane laboratory static small sample test to a dynamic pilot test, optimizing the process parameters to improve the reaction efficiency and move forward to commercialization test.

Matched MeSH terms: Laboratories
Whipworms of south-east Asian rodents are distinct from Trichuris muris

Ribas A, Wells K, Morand S, Chaisiri K, Agatsuma T, Lakim MB, et al.

Parasitol Int, 2020 Aug;77:102128.
PMID: 32330535 DOI: 10.1016/j.parint.2020.102128

The whipworm Trichuris muris is known to be associated with various rodent species in the northern hemisphere, but the species identity of whipworm infecting rodents in the Oriental region remains largely unknown. We collected Trichuris of Muridae rodents in mainland and insular Southeast Asia between 2008 and 2015 and used molecular and morphological approaches to identify the systematic position of new specimens. We discovered two new species that were clearly distinct from T. muris, both in terms of molecular phylogenetic clustering and morphological features, with one species found in Thailand and another one in Borneo. We named the new species from Thailand as Trichuris cossoni and the species from Borneo as Trichuris arrizabalagai. Molecular phylogeny using internal transcribed spacer region (ITS1-5.8S-ITS2) showed a divergence between T. arrizabalagai n. sp., T. cossoni n. sp. and T. muris. Our findings of phylogeographically distinct Trichuris species despite some globally distributed host species requires further research into the distribution of different species, previously assumed to belong to T. muris, which has particular relevance for using these species as laboratory model organisms.

Matched MeSH terms: Laboratories
Fulltext Isolation of Streptococcus cuniculi from corneal lesion in laboratory-raised mice

Tiong V, Loong SK, Mohamad Wali HA, Tan KK, Jee PF, Lim FS, et al.

J Vet Med Sci, 2021 Mar 05;83(2):280-284.
PMID: 33441499 DOI: 10.1292/jvms.20-0070

Corneal lesions appearing as white mass beneath intact epithelium, with ocular discharge in one mouse, was observed in a batch of laboratory-raised BALB/c mice (n=9 of 56). The affected mice remained active, well-groomed and had normal appetite. Isolates recovered from swab cultures of the external and internal contents of the eye had partial 16S rRNA gene sequence of 99.1% similarity to Streptococcus cuniculi. No previous report of S. cuniculi infection in laboratory rodents has been presented. The isolate was susceptible to all antibiotics tested. We suggest S. cuniculi is an opportunistic bacteria in laboratory mice but are uncertain of its source. Our findings revealed that S. cuniculi is able to colonize laboratory mice and should be considered when mice present with eye lesion or ocular discharge.

Matched MeSH terms: Laboratories
Guidelines on safe laboratory practice (version 1/2004)

College of Pathologists, Academy of Medicine of, Malaysia

Malays J Pathol, 2005 Jun;27(1):71-2.
PMID: 16676698
Matched MeSH terms: Laboratories/standards*
Laboratory accidents--a matter of attitude

Karim N, Choe CK

Malays J Pathol, 2000 Dec;22(2):85-9.
PMID: 16329540

This is a prospective study on accidents occurring in the Pathology laboratories of Hospital Ipoh over the 3-year period from January 1996 to October 1999. 15 mishaps were recorded. The location of the accidents were the histology (40%), microbiology (33%), haematology (20%) and cytology (7%) laboratories. No mishaps were reported from the clinical chemistry, blood bank and outpatient laboratories. Cuts by sharp objects were the most common injuries sustained (47%) followed by splashes and squirts by fluid such as blood or chemicals (27%). There was 1 case each of contact with biohazardous fluid, burn, allergy and accidental drinking of disinfectant. 67% of the accidents involved medical laboratory technicians, 20% involved attendants and the rest were medical officers and the junior laboratory technicians. Although the accidents reported appeared trivial, it is vital to document them and bring them to the attention of all concerned in the laboratory, in order to prevent major accidents and also because of medico-legal implications. The role of the Laboratory Safety Committee cannot be overemphasised. Modification of staff attitude is considered an important remedial goal.

Matched MeSH terms: Laboratories, Hospital*
The pattern of bacteriological cultures in a state laboratory

Lim Swee Eng, Bin Mohamed Hussain AH

Med J Malaysia, 1973 Dec;28(2):103-8.
PMID: 4276266
Matched MeSH terms: Laboratories*
Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein

Roslan AA, Tayyab S

Biochem Mol Biol Educ, 2019 03;47(2):156-160.
PMID: 30629781 DOI: 10.1002/bmb.21207

A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM) concentrations produced a consecutive decrease in the protein's fluorescence. Treatment of the fluorescence quenching data according to the Stern-Volmer equation yielded the values of the Stern-Volmer constant (Ksv ) and bimolecular quenching rate constant (kq ), whereas values of the binding constant (Ka ) and number of binding sites (n) were obtained from the double logarithmic plot. This experiment provides an exciting opportunity for undergraduate students to independently perform ligand binding studies with a protein, in addition to providing the means for the determination of their binding parameters. © 2019 International Union of Biochemistry and Molecular Biology, 47(2): 156-160, 2019.

Matched MeSH terms: Laboratories*
Sunway Medical Laboratory Quality Control Plans Based on Six Sigma, Risk Management and Uncertainty

Jairaman J, Sakiman Z, Li LS

Clin. Lab. Med., 2017 Mar;37(1):163-176.
PMID: 28153364 DOI: 10.1016/j.cll.2016.09.013

Sunway Medical Centre (SunMed) implemented Six Sigma, measurement uncertainty, and risk management after the CLSI EP23 Individualized Quality Control Plan approach. Despite the differences in all three approaches, each implementation was beneficial to the laboratory, and none was in conflict with another approach. A synthesis of these approaches, built on a solid foundation of quality control planning, can help build a strong quality management system for the entire laboratory.

Matched MeSH terms: Laboratories/standards*
A large exposure to Brucella melitensis in a diagnostic laboratory

Sam IC, Karunakaran R, Kamarulzaman A, Ponnampalavanar S, Syed Omar SF, Ng KP, et al.

J Hosp Infect, 2012 Apr;80(4):321-5.
PMID: 22237130 DOI: 10.1016/j.jhin.2011.12.004

Brucella species are easily transmitted by aerosols and can be acquired in the laboratory.

Matched MeSH terms: Laboratories, Hospital*
Fulltext Reliability and validity of job content questionnaire for university research laboratory staff in Malaysia

Nehzat F, Huda BZ, Tajuddin SH

Southeast Asian J Trop Med Public Health, 2014 Mar;45(2):481-9.
PMID: 24968690

Job Content Questionnaire (JCQ) has been proven a reliable and valid instrument to assess job stress in many countries and among various occupations. In Malaysia, both English and Malay versions of the JCQ have been administered to automotive workers, schoolteachers, and office workers. This study assessed the reliability and validity of the instrument with research laboratory staff in a university. A cross sectional study was conducted among 258 research laboratory staff in Universiti Putra Malaysia (UPM). Malaysian laboratory staff who have worked for at least one year were randomly selected from nine faculties and institutes in the university that have research laboratory. A self-administered English and Malay version of Job Content Questionnaire (JCQ) was used. Three major scales of JCQ: decision latitude, psychological job demands, and social support were assessed. Cronbach's alpha coefficients of two scales were acceptable, decision latitude and psychological job demands (0.70 and 0.72, respectively), while Cronbach's alpha coefficient for social support (0.86) was good. Exploratory factor analysis showed five factors that correspond closely to the theoretical construct of the questionnaire. The results of this research suggest that the JCQ is reliable and valid for examining psychosocial work situations and job strain among research laboratory staff. Further studies should be done for confirmative results, and further evaluation is needed on the decision authority subscale for this occupation.

Matched MeSH terms: Laboratories/manpower*

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