Cytotoxicity in freshwater fishes induced by industrial effluents and dyes is a global issue. Trypan blue dye has many applications in different sectors, including laboratories and industries. This study determines to detect the cytotoxic effects of trypan blue dye in vivo. The objective of this study was to estimate the sub-lethal effects of azodye in fish. Cirrhinus mrigala, a freshwater fish, was exposed to three different grading concentrations of dye 5 mg/L, 10 mg/L, and 20 mg/L in a glass aquarium. Significant (p
In the current study, we have synthesized an imidazolium based cross-linked polymer, namely, 1-vinyl-3-ethylimidazolium bis(trifluoromethylsulfonyl)imide (poly[veim][Tf2N]-TRIM) using trimethylolpropane trimethacrylate as cross linker, and demonstrated its efficiency for the removal of two extensively used ionic dyes—methylene blue and orange-II—from aqueous systems. The detailed characterization of the synthesized poly[veim][Tf2N]-TRIM was performed with the help of 1H NMR, TGA, FT-IR and FE-SEM analysis. The concentration of dyes in aqueous samples before and after the adsorption process was measured using an UV-vis spectrophotometer. The process parameters were optimised, and highest adsorption was obtained at a solution pH of 7.0, adsorbent dosage of 0.75 g/L, contact time of 7 h and dye concentrations of 100 mg/L and 5.0 mg/L for methylene blue and orange-II, respectively. The adsorption kinetics for orange-II and methylene blue were well described by pseudo-first-order and pseudo−second-order models, respectively. Meanwhile, the process of adsorption was best depicted by Langmuir isotherms for both the dyes. The highest monolayer adsorption capacities for methylene blue and orange-II were found to be 1212 mg/g and 126 mg/g, respectively. Overall, the synthesized cross-linked poly[veim][Tf2N]-TRIM effectively removed the selected ionic dyes from aqueous samples and provided >90% of adsorption efficiency after four cycles of adsorption. A possible adsorption mechanism between the synthesised polymeric adsorbent and proposed dyes is presented. It is further suggested that the proposed ionic liquid polymer adsorbent could effectively remove other ionic dyes and pollutants from contaminated aqueous systems.
The hazardous dyes on mixing with water resources are affecting many life forms. Granite stone is popular worldwide for decorating floors, making other forms of decorative materials and items. Granite stone powder waste can be obtained free of cost from marble factories as factories spend on the disposal of this waste. In the present study, novel granite stone powder waste composite has been prepared and utilized for the effective removal of Terasil dye. Two types of granite including gray granite and white granite were used in pure, calcinized, and chemically modified forms. Freundlich adsorption isotherm model best explained the adsorption mechanism of dye removal using granite composites as compared to other adsorption isothermal models. Characterization techniques like scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy were used for the determination of morphological features and functional groups of granite composites. The obtained results were statistically analyzed using analysis of variance (ANOVA) along with the post hoc Tukey test. An extraordinarily high Terasil dye uptake capacity (more than 400 mg/g) was exhibited by granite composites prepared using sodium metasilicate. The synthesized novel nano-constructed composites provided a viable strategy as compared to the pure granite stone for dye removal from wastewater water.
A secure aquatic environment is essential for both aquatic and terrestrial life. However, rising populations and the industrial revolution have had a significant impact on the quality of the water environment. Despite the implementation of strong and adapted environmental policies for water treatment worldwide, the issue of organic dyes in wastewater remains challenging. Thus, this study aimed to develop an efficient, cost-effective, and sustainable material to treat methylene blue (MB) in an aqueous environment. In this research, maize extract solution (MES) was utilized as a green cross-linker to induce precipitation, conjugation, and enhance the adsorption performance of graphene oxide (GO) cross-linked with durian shell activated carbon (DSAC), resulting in the formation of a GO@DSAC composite. The composite was investigated for its adsorptive performance toward MB in aqueous media. The physicochemical characterization demonstrated that the cross-linking method significantly influenced the porous structure and surface chemistry of GO@DSAC. BET analysis revealed that the GO@DSAC exhibited dominant mesopores with a surface area of 803.67 m2/g. EDX and XPS measurements confirmed the successful cross-linking of GO with DSAC. The adsorption experiments were well described by the Harkin-Jura model and they followed pseudo-second order kinetics. The maximum adsorption capacity reached 666.67 mg/g at 318 K. Thermodynamic evaluation indicated a spontaneous, feasible, and endothermic in nature. Regenerability and reusability investigations demonstrated that the GO@DSAC composite could be reused for up to 10 desorption-adsorption cycles with a removal efficiency of 81.78%. The selective adsorptive performance of GO@DSAC was examined in a binary system containing Rhodamine B (RhB) and methylene orange (MO). The results showed a separation efficiency (α) of 98.89% for MB/MO and 93.66% for MB/RhB mixtures, underscoring outstanding separation capabilities of the GO@DSAC composite. Overall, the GO@DSAC composite displayed promising potential for the effective removal of cationic dyes from wastewater.
This work aims to apply the use of food-grade algae (FGA) composited with chitosan-benzaldehyde Schiff base biopolymer (CHA-BD) as a new adsorbent (CHA-BA/FGA) for methyl violet 2B (MV 2B) dye removal from aqueous solutions. The effect of three processing variables, including CHA-BA/FGA dosage (0.02-0.1 g/100 mL), pH solution (4-10), and contact duration (10-120 min) on the removal of MV 2B was investigated using the Box-Behnken design (BBD) model. Kinetic and equilibrium dye adsorption profiles reveal that the uptake of MV 2B dye by CHA-BA/FGA is described by the pseudo-second kinetics and the Langmuir models. The thermodynamics of the adsorption process (ΔG°, ΔH°, and ΔS°) reveal spontaneous and favorable adsorption parameters of MV 2B dye onto the CHA-BA/FGA biocomposite at ambient conditions. The CHA-BA/FGA exhibited the maximum ability to absorb MV 2B of 126.51 mg/g (operating conditions: CHA-BA/FGA dose = 0.09 g/100 mL, solution pH = 8.68, and temperature = 25 °C). Various interactions, including H-bonding, electrostatic forces, π-π stacking, and n-π stacking provide an account of the hypothesized mechanism of MV 2B adsorption onto the surface of CHA-BA/FGA. This research reveals that CHA-BA/FGA with its unique biocomposite structure and favorable adsorption properties can be used to remove harmful cationic dyes from wastewater.
This present work targets the production of an eco-friendly adsorbent (hereinafter KA/CEL) from kaolin clay functionalized with cellulose extract obtained from peanut shells. The adsorbents were used for decolorization of two different types of organic dyes (cationic: methylene blue, MB; anionic: Congo red, CR) from an aqueous environment. Several analytical methods, including Brunauer-Emmett-Teller (surface properties), Fourier Transforms infrared (functionality), scanning electron microscope, Energy dispersive X-Ray (morphology), and pHpzc test (surface charge), were used to attain the physicochemical characteristics of KA/CEL. The Box-Behnken Design (BBD) was applied to determine the crucial factors affecting adsorption performance. These included cellulose loading at 25 %, an adsorbent dose of 0.06 g, solution pH set at 10 for MB and 7 for CR, a temperature of 45 °C, and contact times of 12.5 min for MB and 20 min for CR dye. The adsorption data exhibited better agreement with the pseudo-second-order kinetic and Freundlich models. The Langmuir model estimated the monolayer capacity to be 291.5 mg/g for MB and 130.7 mg/g for CR at a temperature of 45 °C. This study's pivotal finding underscores the promising potential of KA/CEL as an effective adsorbent for treating wastewater contaminated with organic dyes.
In the present study, spent tea leaves (STL) were used as a new non-conventional and low-cost adsorbent for the cationic dye (methylene blue) adsorption in a batch process at 30 degrees C. Equilibrium sorption isotherms and kinetics were investigated. The experimental data were analyzed by the Langmuir, Freundlich and Temkin models of adsorption. The adsorption isotherm data were fitted well to the Langmuir isotherm and the monolayer adsorption capacity was found to be 300.052mg/g at 30 degrees C. The kinetic data obtained at different initial concentrations were analyzed using pseudo-first-order, pseudo-second-order and intraparticle diffusion equations. The results revealed that the spent tea leaves, being waste, have the potential to be used as a low-cost adsorbent for the removal of methylene blue from aqueous solutions.
A microscope slide acting as a passive waveguide was coated by three separate poly(vinyl alcohol) films that were doped with Coumarin 460, Disodium Fluorescein, and Rhodamine 640 perchlorate. On collinear pumping by a nitrogen laser, these dyes furnished primary red-green-blue laser emissions that were collected and waveguided by the microscope slide but exited from both ends. Frosting the waveguide exit introduced light scattering at the glass-air interface and spatially overlaid the red-green-blue laser emissions that emerged as a uniform white-light beam.
The increasing and widespread use of synthetic food dyes raises health concerns and earlier reports suggest that certain food dyes might be harmful for human health. In this study, we have investigated the effect of three commonly used food dyes on human liver cell line, HepG2. Our findings suggest that these experimental food dyes significantly affect cell viability and this effect can be worsen in hyperglycemic condition. Accumulation of cellular fat was significantly higher in presence of these dyes. Expression pattern of the gene involved in regulating apoptosis suggests that that the observed cell death could be attributed to the activation of apoptotic pathway. These findings suggest that these experimental dyes might exert synergistic toxicity in hyperglycemia that need to be confirmed using suitable in vivo models.
The removal of methyl orange (MO) dye has been studied using TiO2/chitosan-montmorillonite (TiO2/Cs-MT) bilayer photocatalyst which also functions as an adsorbent. The dye removal experiments were conducted in the dark and under UV-Vis light irradiation via adsorption and photocatalysis-adsorption processes, respectively. The adsorption modelings were employed on the dark experimental data and compared with the immobilized and suspended Cs-Mt counterparts. It was found that the bilayer photocatalyst closely followed the adsorption properties of immobilized Cs-Mt which obeyed the pseudo-second-order kinetic and film diffusion models. Fluorescent analysis revealed that the charge separation was enhanced in the presence of Cs-Mt as a sub-layer of TiO2. Under light irradiation, the photocatalytic activity of TiO2/Cs-MT corresponded to its adsorption counterpart trend and was optimized at pH 6.5 and 20 mg L-1 of MO dye solution. High removal efficiency and synergism of MO by TiO2/Cs-MT over TiO2 single layer were observed throughout the 10 cycles of application due to contribution of adsorption of Cs-Mt sub-layer and photocatalysis by TiO2 top layer.
Methyl orange is one of the anionic dyes and is a major pollutant from textile industry that enters both aquatic and atmospheric systems. In this research, methyl orange was degraded using TiO2 powder and immobilized TiO2 on glass. Titanium tetra-isopropoxide (TTIP) was used for preparation of TiO2 powder using soft chemistry method, and it was immobilized on glass via paste-gel coating method. The prepared photocatalysts were characterized by XRD and SEM. Highly crystalline anatase TiO2 powder photocatalyst was obtained. Meanwhile, immobilized TiO2 was less crystalline and agglomerated onto the glass surface. TiO2 powder had higher degradation rate (71%) compared to immobilized TiO2 (52%) due to its chemical stability and larger amount of photocatalyst contacted with methyl orange during the degradation process.
Of late, dyeing fabrics with natural dyes have become an attraction because of its eco-friendly and less threatening disposition towards humankind. In the textile colouration industry, natural dyes play an important role because of the need for replacement synthetic dyes which have a great deal of tension with the environmental issues. This study focuses on the colour shade, colour coordinates, and fastness properties of dyed silk fabric from tagetes erecta (Mexican Marigold flower) using the water boiling extraction method. The dyeing was carried out using lemon juice as a natural mordant through the simultaneous mordanting method, using two different dyeing methods: infrared (IR) dyeing and exhaustion dyeing. The shades produced for exhaustion dyed fabric is light-yellow compared to the IR dyed fabric, which is medium-light yellow. These shades were confirmed with the CIELAB colour coordinates, L*a*b* values. The colourfastness to washing, perspiration, rubbing, and light of the fabrics were conducted to investigate the performance of the dye and mordant on the dyed silk fabrics. The colourfastness properties of the dyed silk fabric using infrared (IR) dyeing technique have better performance than using exhaustion dyeing technique.
Routine diagnosis of intestinal microsporidiosis in clinical diagnostic laboratories relies mostly on detection of microsporidial spores via special staining and microscopic techniques. This paper describes the comparative evaluation of Calcofluor White M2R method, with modified Gram-chromotrope Kinyoun method as the reference standard. One hundred and six stool samples were examined for the presence of microsporidial spores. Sensitivity, specificity, positive and negative predictive values of the Calcofluor White M2R method compared to the reference technique were 95.2%, 4.3%, 78.2% and 20.0%, respectively. The positive predictive value (PPV) was 78.2% and the negative predictive value (NPV) was 20.0%. Despite low specificity of the CFW method due to its ability to stain chitinous wall of microorganisms, the presence of distinct deep-blue horizontal or equitorial stripes in microsporidial spores in modified Gram-chromotrope Kinyoun would likely reduce the false positive results obtained in the Calcofluor White M2R. Hence, the simultaneous use of these two methods would give better performance and accuracy for the detection of microsporidial spores in patients with intestinal microsporidiosis.
Decalcified permanent teeth were sectioned and stained with Van Gieson and Gomori trichome dyes. Sections dyed with the Van Gieson dye did not produce any zones in dentine but with the Gomori trichome dye, four different zones of dentine were produced. Zone 1 begins at the predentine-dentine junction while zone 4 ends at the enamel - dentine junction. In zone 1, the intertubular dentine was stained clearly while in zone 3 intense staining of the peritubular dentine was observed. The result of this study supports the previous findings reported by other workers that the formation of intertubular dentine takes place in zone 1 while peritubular dentine occurs in zone 3.
The industrial discharge of dye pollutant contaminated wastewater is the major cause of water and soil pollution. Photocatalysis is a promising and green remediation technology, which has received widespread attention in the remediation of hazardous dyes from aqueous environment and convert them into harmless compounds. Herein, we report the synthesis of chitosan (CS) functionalized bismuth oxychloride/zinc oxide (BiOCl/ZnO) nanocomposite by a modified hydrothermal route. The physiochemical characterization revealed that the synthesized nanocomposite have crystalline, agglomerated spherical along with rod shaped morphology and size range from 35 to 160 nm. FTIR peaks at 825, 727, 662 and 622 cm-1 specified the presence of BiO and ZnO bonds, whereas peak at 1635 cm-1 revealed the existence of amine groups which confirms the presence of CS in the synthesized CS-BiOCl/ZnO nanocomposite. Catalytic property of synthesized nanocomposite was evaluated by the degradation of Congo red (CR) under UV-light irradiation. CR dye degradation percentage was found to be 93 % within a short period of 40 min by utilizing UV-light. Furthermore, reusability of CS-BiOCl/ZnO photocatalyst was also investigated, and it remained significant photocatalytic activity after three consecutive cycles. Hence, the results obtained in this study revealed that CS-BiOCl/ZnO nanocomposite can be used as a potential photocatalyst to remediate organic pollutants in various industries.
The inherent toxicity, mutagenicity and carcinogenicity of dyes that are discharged into aquatic ecosystems, harming the health of humans and animals. ZIF-8 based composites are regarded as good adsorbents for the breakdown of dyes in order to remove or degrade them. In the course of this research, metal-organic framework materials known as ZIF-8 and its two stable composites, ZIF-8/BiCoO3 (MZBC) and ZIF-8/BiYO3 (MZBY), were produced via a hydrothermal process and solvothermal process, respectively, for the dangerous Congo red (CR) dye removal from the solution in water using adsorption method. According to the findings, the most significant amount of CR dye that could be adsorbed is onto MZBC, followed by MZBY and ZIF-8. The pseudo-second-order kinetic model was used effectively to match the data for adsorption behavior and was confirmed using the Langmuir isotherm equation. There is a possibility that the pH and amount of adsorbent might influence the adsorption behavior of the adsorbents. According to the experiment results, the technique featured an endothermic adsorption reaction that spontaneously occurred. The higher adsorption capability of MZBC is because of the large surface area. This results in strong interactions between the functional groups on the surface of MZBC and CR dye molecules. In addition to the electrostatic connection between functional group Zn-O-H on the surface of ZIF-8 in MZBC and the -NH2 or SO3 functional group areas in CR molecules, it also includes the strong π-π interaction of biphenyl rings.
Chitosan/PVA/Na-titanate/TiO2 composite was synthesized by solution casting method. The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test. Incorporation of Na-titanate shown decrease of crystallinity for chitosan but increase water stability. However, the composite structure was deteriorated with considerable weight loss in acidic medium. Two anionic dyes, methyl orange and congo red were used for the adsorption test. The adsorption behavior of the composites were described by pseudo-second-order kinetic model and Lagergren-first-order model for methyl orange and congo red, respectively. For methyl orange, adsorption was started with a promising decolorization rate. 99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase. On the other hand, for the congo red the composite having higher chitosan and Na-titanate showed an efficient removal capacity of 95.76%. UV-vis results showed that the molecular backbone of methyl orange and congo red was almost destroyed when equilibrium was obtained, and the decolorization rate was reaching 100%. Kinetic study results showed that the photocatalytic degradation of methyl orange and congo red could be explained by Langmuir-Hinshelwood model. Thus, chitosan/PVA/Na-titanate/TiO2 possesses efficient adsorptivity and photocatalytic property for dye degradation.
Mixed dye consists of six commercial dyes and textile effluents from cotton dyeing process were treated by electrochemical-assisted photodegradation under halogen lamp illumination. Two types of effluents were collected which are samples before and after undergone pre-treatment at the factory wastewater treatment plant. The photodegradation process was studied by evaluating the changes in concentration employing UV-vis spectrophotometer (UV-vis) and total organic carbon (TOC) analysis. The photoelectrochemical degradation of mixed dye was found to follow the Langmuir Hinshelwood pseudo-first order kinetic while pseudo-second order kinetic model for effluents by using TOC analyses. The chemical oxygen demand (COD) and biochemical oxygen demand (BOD) values of mixed dye and raw effluents were reported. Photoelectrochemical characteristic of pollutants was studied using the cyclic voltammetry technique. Raw effluent was found to exhibit stronger reduction behaviour at cathodic bias potential but slightly less photoresponse at anodic bias than mixed dye.
Oil palm empty fruit bunch (EFB) fibers were employed to remove dyes from aqueous solutions via adsorption approaches. The EFB fibers were modified using citric acid (CA) and polyethylenimine (PEI) to produce anionic and cationic adsorbents, respectively. The CA modified EFB fibers (CA-EFB) and PEI-modified EFB fibers (PEI-EFB) were used to study the efficiency in removing cationic methylene blue (MB) and anionic phenol red (PR) from aqueous solutions, respectively, at different pHs, temperatures and initial dye concentrations. The adsorption data for MB on the CA-EFB fitted the Langmuir isotherm, while the adsorption of PR on the PEI-EFB fitted the Freundlich isotherm, suggesting a monolayer and heterogeneous adsorption behavior of the adsorption processes, respectively. Both modified fibers can be regenerated up to seven adsorption/desorption cycles while still providing as least 70% of the initial adsorption capacity.