In vitro Lead (Pb(2+)) binding capacity of two probiotic bacteria strains, namely Bifidobacterium longumBB79 and Lactobacillus pentosusITA23, was assessed following incubation with the intestinal contents (IC) of laying hens. Results of this study demonstrated that IC treatment significantly enhanced (P<0.01) Pb(2+) binding capacity of both bacterial strains. Fourier transform infrared analysis indicated that several functional groups (O-H or N-H, C-H, C˭O, C-O, and C-O-C) on the bacteria cell wall involved in metal ion binding were altered after IC incubation, and new groups appeared between the 3700cm(-1) and 4000cm(-1)bands. Transmission electron microscopy demonstrated that after incubation with IC, unidentified IC components created new binding sites on the bacterial cell surface. These particles also changed the mechanism of Pb(2+) binding of the two strains from intracellular accumulation to extracellular adsorption.
Layered double hydroxide (LDH) with Mg/Al molar ratio of 4/1 (MAN-4) was synthesized by co-precipitation and followed by hydrothermal method. The compound was allowed to undergo ion exchange with K2HPO4 for 48 hours to produce MgAlHPO4 (MAHP-4). The solid produced was characterized using X-ray diffraction (XRD) and Fourier Transform Infrared spectroscopy (FTIR). Adsorption of copper solution by MAHP-4 was carried out using batch experiment by mixing the copper solution and the sorbent MAHP-4. The effects of
various parameters such as contact time, pH, adsorbent dosage and initial concentration were investigated. The optimum pH for copper removal was found to be 4 and the optimum time of copper removal was found at 4 hours. The isotherm data was analysed using model isotherm Langmuir with the correlation coefficient of 0.999 was recorded. The maximum adsorption capacity, Qo (mg/g) of 142.8 mg/g was also recorded from the Langmuir isotherm. The remaining copper solution was determined by using EDXRF (Energy Dispersive XRay Fluorescence spectrometry) model MiniPal 4 (PAN analytical). The results in this study indicate that MAHP-4 has potential as an effective adsorbent for removing copper from aqueous solution.
Edible bird's nest (EBN) is a precious food made from the solidified saliva of swiftlets. EBN from three types of origin, namely production, swiftlet species and geographical were characterised based on its nutritional composition, physicochemical properties and antioxidant properties. Proximate composition, total phenolic content (TPC) and antioxidant activities were determined following official methods, while mineral and heavy metal contents were obtained by respective atomic adsorption spectrometry (AAS) and inductively coupled plasma-mass spectrometry (ICP-MS). Amino acids profile and sialic acid were determined using high performance liquid chromatography (HPLC). Calcium and sodium were the major elements in EBN samples at averages of 17,267 mg/kg and 13,681 mg/kg, respectively. Despite protein contents were not significantly different; interestingly the total amino acids in A. fuciphagus EBN, 64.57 g/100 g was found to be 23% higher than in A. maximus EBN. EBN from house, A. fuciphagus and Peninsular Malaysia had greater antioxidant activities, 2.33-3.49 mg AAE/g and higher sialic acid, 13.57 g/100 g while those from cave, A. maximus and East Malaysia contained more minerals like calcium and magnesium. The 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and ferric ion reducing antioxidant power (FRAP) of house, A. fuciphagus and Peninsular Malaysia EBNs were approximately 2 times greater than the others. All samples were complied with the Malaysian Standard MS 2334:2011, except for mercury and nitrite. The overall findings suggest that the quality of EBN was varied following the production, species and geographical origins.
Nickel (Ni), cobalt (Co), and zinc (Zn) loaded on fibrous silica KCC-1 was investigated for CO2 methanation reactions. Ni/KCC-1 exhibits the highest catalyst performance with a CH4 formation rate of 33.02 × 10-2 molCH4 molmetal-1 s-1, 1.77 times higher than that of Co/KCC-1 followed by Zn/KCC-1 and finally the parent KCC-1. A pyrrole adsorption FTIR study reveals shifting of perturbed N-H stretching decreasing slightly with the addition of metal oxide, suggesting that the basic sites of catalyst were inaccessible due to metal oxide deposition. The strengths of basicity were found to follow sthe equence KCC-1, Ni/KCC-1, Zn/KCC-1, and Co/KCC-1. The data were supported by N2 adsorption desorption analysis, where Co/KCC-1 displayed the greatest reduction in total surface area whereas Ni/KCC-1 displayed the least reduction. The elucidation of difference mechanism pathways has also been studied by in situ IR spectroscopy studies to determine the role of different metal oxides in CO2 methanation. It was discovered that Ni/KCC-1 and Co/KCC-1 follow a dissociative mechanism of CO2 methanation in which the CO2 molecule was dissociated on the surface of the metal oxide before migration onto the catalyst surface. This was confirmed by the evolution of a peak corresponding to carbonyl species (COads) on a metal oxide surface in FTIR spectra. Zn/KCC-1, on the other hand, showed no such peak, indicating associative methanation pathways where a hydrogen molecule interacts with an O atom in CO2 to form COads and OH. These results offers a better understanding for catalytic studies, particularly in the field of CO2 recycling.
An agricultural waste, the cocoa pod husk was chemically modified using a dehydrating agent, zinc chloride (ZnCl2), carbonised and used for the remediation of acid dyes in an aqueous solution. The targeted acid dyes are: (i) Acid Violet 17 (AV17); (ii) Acid Yellow 36 (AY36); and (iii) Acid Blue 29 (AB29). The physicochemical properties of the zinc chloride-modified cocoa pod husk-based carbon (ZCPHC) were characterised by ash content, bulk density, pH slurry, pHpzc and Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-Ray (EDX) analysis. The bulk density and ash content of the prepared carbon is 0.55 g cm-1 and 7.0% respectively. The photograph of SEM shows distinct changes at the ZCPHC carbon surface as it has large pores formed due to ZnCl2 modification. The adsorption tests were performed in a batch adsorption system using an aqueous solution of the understudy acid dyes. The influence of pH and dose of an adsorbent on the acid dye uptake was investigated and discussed. The adsorption was in favour at acidic condition with maximum removal observed at pH 2. The removal efficiency of the aqueous acid dye solution increased with the increase in adsorbent dosage. The kinetic experiment showed equilibrium time is less than 40 minutes and the kinetic data for all three understudy acid dyes fitted well with the pseudo-second-order model with a correlation coefficient (R2) values above 0.98.
Clean water is very important for health and well-being of humans and ecosystem. However, over the year, a billion tons of industrial waste, fertilizers and chemical waste were dumped untreated into water bodies, such as rivers, lake and oceans contributing towards water pollution, then threatening human health and ecosystem. Hence, the need for clean water has urged scientists to research and find solutions for improving water quality. Application of nanoparticles in wastewater treatment improves the environmental quality by elimination of harmful pollutants in wastewater. Magnetite is one of the nanoparticles used in wastewater treatment because of its specific large surface area, high reactivity in adsorption and recoverable from treated water via magnetic separation technology. Preparation method of magnetite nanoparticles is the important key to its adsorption efficiency.
In this study, carbon species were grown on the surface of Ni-impregnated powder activated carbon to form a novel hybrid carbon nanomaterial by chemical vapor deposition. The carbon nanomaterial was obtained by the precipitation of the methane elemental carbon atoms on the surface of the Ni catalyst. The physiochemical properties of the hybrid material were characterized to illustrate the successful growth of carbon species on the carbon substrate. The response surface methodology was used for the evaluation of adsorption parameters effect such as pH, adsorbent dose and contact time on the percentage removal of MB dye from aqueous solution. The optimum conditions were found to be pH = 11, adsorbent dose = 15 mg and contact time of 120 min. The material we prepared showed excellent removal efficiency of 96% for initial MB concentration of 50 mg/L. The adsorption of MB was described accurately by the pseudo-second-order model with R2 of 0.998 and qe of 163.93 (mg/g). The adsorption system showed the best agreement with Langmuir model with R2 of 0.989 and maximum adsorption capacity (Qm) of 250 mg/g.
A combination of phosphoric acid (H3PO4) 20% v/v impregnation and carbonization method was employed to convert honeydew rind into activated carbons (ACPHDR) for Zn(II) and Cr(III) removal aqueous solution. The characterization of ACPDHR by N2 sorption, iodine number and Boehm analysis result 1272 m2/g surface area, 1174 mg/g and 1.13 mmol/g total acidic functional groups respectively. Fourier transform infrared (FTIR) and Field emission scanning electron microscopy-electron dispersed microscopy (FESEM-EDX) analysis of unloaded and metal-loaded carbon showed shifted of significance peaks and the changes of surface morphology of the sorbent. The adsorption was optimized at pH, shaking duration, initial metal concentration and mass of adsorbent of 5.5, 40 min and 500 mg/L, 0.4 g for Zn(II) and 4, 40 min, 1000 mg/L, 0.1 g for Cr(III) removal. It is concluded that the metal removal was influenced by pH solution, contact time, initial metal concentration and mass of adsorbent. The highest removal of Zn(II) and Cr(III) was observed at 84.24% and 90.10% respectively. Waste from honeydew will be benefited from this research which offer a cheaper alternative precursor to coal based activated carbons.
The chitosan/polyvinyl alcohol/TiO2 composite was synthesized. Two different degrees of deacetylation of chitosan were prepared by hydrolysis to compare the effectiveness of them. The composite was analyzed via field emission scanning electron microscopy, Fourier transform infrared, X-ray diffraction, thermal gravimetric analysis, weight loss test and adsorption study. The FTIR and XRD results proved the interaction among chitosan, PVA and TiO2 without any chemical reaction. It was found that, chitosan with higher degree of deacetylation has better stability. Furthermore, it also showed that higher DD of chitosan required less time to reach equilibrium for methyl orange. The adsorption followed the pseudo-second-order kinetic model. The Langmuir and Freundlich isotherm models were fitted well for isotherm study. Adsorption capacity was higher for the composite containing chitosan with higher DD. The dye removal rate was independent of the dye's initial concentration. The adsorption capacity was increased with temperature and it was found from reusability test that the composite containing chitosan with higher DD is more reusable. It was notable that adsorption capacity was even after 15 runs. Therefore, chitosan/PVA/TiO2 composite can be a very useful material for dye removal.
In this work, a human hair-derived high surface area porous carbon material (HHC) was prepared using potassium hydroxide activation. The morphology and textural properties of the HHC structure, along with its adsorption performance for tetracycline (TC) antibiotics, were evaluated. HHC showed a high surface area of 1505.11m(2)/g and 68.34% microporosity. The effects of most important variables, such as initial concentration (25-355mg/L), solution pH (3-13), and temperatures (30-50°C), on the HHC adsorption performance were investigated. Isotherm data analysis revealed the favorable application of the Langmuir model, with maximum TC uptakes of 128.52, 162.62, and 210.18mg/g at 30, 40, and 50°C, respectively. The experimental data of TC uptakes versus time were analyzed efficiently using a pseudo-first order model. Porous HHC could be an efficient adsorbent for eliminating antibiotic pollutants in wastewater.
Agricultural waste obtained from Elaeis guineensis mid ribs can provide a veritable source of materials which can be used as precursor materials for the production of pharmaceutical grade activated charcoal. The pore size and surface morphology of activated charcoal defines the types of molecules that could be adsorbed unto it, as surface morphology plays a significant role in determining the surface availability and areas of adsorption. The activated charcoal samples prepared from Elaeis guineensis via either physical or chemical activation was characterized via surface area using the BET method and subsequently pore structure and size analyzed by scanning electron microscopy (SEM). Physically activated Elaeis guineensis fronds activated with nitrogen gas had wide spread microporosity with micropore volume of 0.232 cc/g compared to the chemically activated with 1M and 3M phosphoric acid respectively. The commercial activated charcoal/metronidazole combination in the in vitro-pharmacodynamic model reflected no re-growth after 4 hours, however for charcoal formulated from Elaeis guineensis via chemical activation with 3M phosphoric acid and metronidazole no regrowth was seen at the second hour and this was maintained throughout the duration of the experiment. Increased macroporosity enhanced bacterial adsorption and this was further facilitated by the presence of antibacterial metronidazole in the in vitro pharmacodynamic model. Activated charcoal produced from agricultural waste obtained from Elaeis guineensis dried mid ribs consisting of increased macroporosity with mixed meso/micro porosity and antibacterial metronidazole form the best model for bacterial adsorption and will be useful in the treatment of diarrhea caused by E. coli O157:H7.
The world water resources are contaminated due to discharge of a large number of pollutants from industrial and domestic sources. A variety of a single and multiple units of physical, chemical, and biological processes are employed for pollutants removal from wastewater. Adsorption is the most widely utilized process due to high efficiency, simple procedure and cost effectiveness. This paper reviews the research work carried out on the use of geopolymer materials for the adsorption of heavy metals and dyes. Geopolymers possess good surface properties, heterogeneous microstructure and amorphous structure. The performance of geopolymers in the removal of heavy metals and dyes is reported comparable to other materials. The pseudo-second order kinetics and Langmuir isotherm models mostly fit to the adsorption data suggesting homogeneous distribution of adsorption sites with the formation of monolayer adsorbate on the surface of geopolymers. Adsorption of heavy metals and dyes onto geopolymers is spontaneous, endothermic and entropy driven process. Future research should focus on the enhancement of geopolymer performance, testing on pollutants other than heavy metals and dyes, and verification on real wastewater in continuous operation.
Widespread applications of phenol in manufacturing industries and oil refineries had resulted in unprecedented leakage of phenol into the environment, which can cause serious health effects such as tissue necrosis and cardiac arrhythmia upon contact or ingestion. Plants exposed to phenol had reduced seed germination index, inhibited growth or even fatality. There are many technologies currently practised to remediate phenol pollution such as physiochemical methods (adsorption to activated carbon and chemical oxidation), biological methods (biodegradation by bacteria or fungus, and soil bioaugmentation), and phytoremediation method (using hairy roots of plants). As physiochemical and microbial phenol degradation are destructive and costly, phytoremediation is widely studied as an alternative phenol remediator which is environmental friendly and cost effective. Microorganisms can detoxify the aromatic xenobiotic through the aerobic or anaerobic pathway. Aerobic degradation of phenol is through either the meta- or ortho-pathway of catechol cleavage while anaerobic degradation occurs through the benzoate pathway. In plants, degradation of phenol is also through catechol cleavage as in microorganisms. However, different enzyme systems were utilised in the different pathways involved.
Adsorption is one of the most efficient ways to remove heavy metal from wastewater. In this study, the adsorptive removal of hexavalent chromium, Cr (VI) from aqueous solution was investigated using natural zeolite, clinoptilolite, in the form of hollow fibre ceramic membrane (HFCM). The HFCM sample was prepared using phase inversion-based extrusion technique and followed by sintering process at different sintering temperatures in the range of 900-1050 °C. The fabricated HFCM was characterised using scanning electron microscopy (SEM), contact angle, water permeability, and mechanical strength for all HFCMs sintered at different temperatures. The adsorption and filtration test of Cr (VI) were performed using an in-house water permeation set up with a dead-end cross-flow permeation test. An asymmetric structure with sponge- and finger-like structures across the cross-section of HFCM was observed using SEM. Based on the characterisation data, 1050 °C was chosen to be the best sintering temperature as the water permeability and mechanical strength of this HFCM were 29.14 L/m2∙h and 50.92 MPa, respectively. The performance of the HFCM in adsorption/filtration was 44% of Cr (VI) removal at the Cr (VI) concentration of 40 mg/L and pH 4. In addition, the mathematical model was also performed in simulating the experimental data obtained from this study. All in all, the natural zeolite-based HFCM has a potential as a single-step Cr (VI) removal by membrane adsorption for the wastewater treatment.
Hydrothermal carbonization of biomass wastes presents a promising step in the production of cost-effective activated carbon. In the present work, mesoporous activated carbon (HAC) was prepared by the hydrothermal carbonization of rattan furniture wastes followed by NaOH activation. The textural and morphological characteristics, along with adsorption performance of prepared HAC toward methylene blue (MB) dye, were evaluated. The effects of common adsorption variables on performance resulted in a removal efficiency of 96% for the MB sample at initial concentration of 25mg/L, solution pH of 7, 30°C, and 8h. The Langmuir equation showed the best isotherm data correlation, with a maximum uptake of 359mg/g. The adsorbed amount versus time data was well fitted by a pseudo-second order kinetic model. The prepared HAC with a high surface area of 1135m(2)/g and an average pore size distribution of 35.5Å could be an efficient adsorbent for treatment of synthetic dyes in wastewaters.
Pharmaceutical residues are emerging pollutants in the aquatic environment and their removal by conventional wastewater treatment methods has proven to be ineffective. This research aimed to develop a three-dimensional reduced graphene oxide aerogel (rGOA) for the removal of diclofenac in aqueous solution. The preparation of rGOA involved facile self-assembly of graphene oxide under a reductive environment of L-ascorbic acid. Characterisation of rGOA was performed by Fourier transform infrared, scanning electron microscope, transmission electron microscopy, nitrogen adsorption-desorption, Raman spectroscopy and X-ray diffraction. The developed rGOA had a measured density of 20.39 ± 5.28 mg/cm3, specific surface area of 132.19 m2/g, cumulative pore volume of 0.5388 cm3/g and point of zero charge of 6.3. A study on the simultaneous interactions of independent factors by response surface methodology suggested dosage and initial concentration as the dominant parameters influencing the adsorption of diclofenac. The highest diclofenac adsorption capacity (596.71 mg/g) was achieved at the optimum conditions of 0.25 g/L dosage, 325 mg/L initial concentration, 200 rpm shaking speed and 30 °C temperature. The adsorption equilibrium data were best fitted to the Freundlich model with correlation coefficient (R2) varying from 0.9500 to 0.9802. The adsorption kinetic data were best correlated to the pseudo-first-order model with R2 ranging from 0.8467 to 0.9621. Thermodynamic analysis showed that the process was spontaneous (∆G = - 7.19 to - 0.48 kJ/mol) and exothermic (∆H = - 12.82 to - 2.17 kJ/mol). This research concluded that rGOA is a very promising adsorbent for the remediation of water polluted by diclofenac.
Dye pollutants from research laboratories are one of the major sources for environmental contamination. In the present study, a nutraceutical industrial fennel seed spent (NIFSS) was explored as potential adsorbent for removal of ethidium bromide (EtBr) from aqueous solution. The adsorbent was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Through batch experiments, the operating variables like initial dye concentration, adsorbent dosage, temperature, contact time, and pH were optimized. Equilibrium data were analyzed using three number of two-parameter and six number of three-parameter isotherm models. The adsorption kinetics was studied using pseudo-first order and pseudo-second order. The diffusion effects were studied by film diffusion, Webber-Morris, and Dumwald-Wagner diffusion models. The thermodynamic parameters; change in enthalpy (ΔHº), entropy (ΔSº), and Gibbs free energy (ΔGº) of adsorption system were also determined and evaluated.
In this work, we reported the synthesis, characterization and adsorption study of two β-cyclodextrin (βCD) cross-linked polymers using aromatic linker 2,4-toluene diisocyanate (2,4-TDI) and aliphatic linker 1,6-hexamethylene diisocyanate (1,6-HDI) to form insoluble βCD-TDI and βCD-HDI. The adsorption of 2,4-dinitrophenol (DNP) on both polymers as an adsorbent was studied in batch adsorption experiments. Both polymers were well characterized using various tools that include Fourier transform infrared spectroscopy, thermogravimetric analysis, Brunauer-Emmett-Teller analysis and scanning electron microscopy, and the results obtained were compared with the native βCD. The adsorption isotherm of 2,4-DNP onto polymers was studied. It showed that the Freundlich isotherm is a better fit for βCD-TDI, while the Langmuir isotherm is a better fit for βCD-HMDI. The pseudo-second-order kinetic model represented the adsorption process for both of the polymers. The thermodynamic study showed that βCD-TDI polymer was more favourable towards 2,4-DNP when compared with βCD-HDI polymer. Under optimized conditions, both βCD polymers were successfully applied on various environmental water samples for the removal of 2,4-DNP. βCD-TDI polymer showed enhanced sorption capacity and higher removal efficiency (greater than 80%) than βCD-HDI (greater than 70%) towards 2,4-DNP. The mechanism involved was discussed, and the effects of cross-linkers on βCD open up new perspectives for the removal of toxic contaminants from a body of water.
The development of new adsorbent has rapidly increased in order to overcome the problem
of waste water treatment from heavy metal pollution. The ability of nickel (II)-ion imprinted
polymer (Ni-IIP) as an alternative adsorbent for the removal of nickel ion from aqueous has
been investigated. The Ni-IIP was prepared via bulk polymerization by using functional
monomers; methylacrylic acid (MAA) with picolinic acid as a co-monomer. Nickel ion was
used as template, AIBN as initiator and EGDMA as cross-linking agent. Non-imprinted control
polymer (NIP) was prepared in the same manner as Ni-IIP but in the absence of nickel
ion. The resultant of Ni-IIP and NIP were characterized by using Fourier Transform Infrared
(FTIR) spectroscopy and Scanning Electron Microscope (SEM). Result showed that, the adsorption
of nickel ion onto Ni-IIP increased as the adsorbent dosage increased and contact
time is prolonged. The adsorption isotherm model for Ni-IIP and NIP were fitted well with
Freundlich and Langmuir, respectively. Kinetic study for both Ni-IIP and NIP were followed
the pseudo-second order, indicates that the rate-limiting step is the surface adsorption that
involves chemisorption. Selectivity studies showed that the distribution coefficient of Ni2+
was higher compared to Zn2+, Mg2+ and Pb2+. The present work has successfully synthesized
Ni-IIP particles with good potential in recognition of Ni2+ ions in an aqueous medium.
As textile production flourishes nowadays, the amount of dyed wastewater entering the
water body has also increased. Dyes could have serious negative impacts to the environment
and also the human health, hence, they need to be removed from the water body. In this
study, layered double hydroxide (LDH) of manganese/aluminium (MnAl) was synthesised
to be used as a potential adsorbent to remove methyl orange (MO) dye due to its unique
lamellar structure which provides LDH with high anion adsorption and exchange ability.
MnAl was synthesized by using co-precipitation method and characterized by powder X-ray
diffraction (PXRD), Fourier-Transform Infrared Spectroscopy (FTIR), Inductively coupled
plasma atomic emission spectroscopy (ICP-AES) and Carbon, Hydrogen, Nitrogen, Sulphur
(CHNS) elemental analysers, and Accelerated Surface Area and Porosity Analyzer (ASAP).
Adsorption studies were conducted at different contact times and dosages of MnAl to evaluate
the performance of MnAl in removing MO from water. Kinetic and isotherm models were
tested using pseudo-first order, pseudo-second order, Langmuir isotherm and Freundlich
isotherm. MnAl LDH was found to be perfectly fitted into pseudo-second order and Langmuir
isotherm.