Displaying publications 401 - 420 of 4087 in total

Abstract:
Sort:
  1. Crystallization and X-ray crystallographic analysis of recombinant TylP, a putative γ-butyrolactone receptor protein from Streptomyces fradiae
    Mohd-Sharif N, Shaibullah S, Givajothi V, Tan CS, Ho KL, Teh AH, et al.
    Acta Crystallogr F Struct Biol Commun, 2017 02 01;73(Pt 2):109-115.
    PMID: 28177322 DOI: 10.1107/S2053230X17001212
    TylP is one of five regulatory proteins involved in the regulation of antibiotic (tylosin) production, morphological and physiological differentiation in Streptomyces fradiae. Its function is similar to those of various γ-butyrolactone receptor proteins. In this report, N-terminally His-tagged recombinant TylP protein (rTylP) was overproduced in Escherichia coli and purified to homogeneity. The rTylP protein was crystallized from a reservoir solution comprising 34%(v/v) ethylene glycol and 5%(v/v) glycerol. The protein crystals diffracted X-rays to 3.05 Å resolution and belonged to the trigonal space group P3121, with unit-cell parameters a = b = 126.62, c = 95.63 Å.
    Matched MeSH terms: Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/chemistry*; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Recombinant Proteins/chemistry
  2. Extraction of proteins from microalgae using integrated method of sugaring-out assisted liquid biphasic flotation (LBF) and ultrasound
    Sankaran R, Manickam S, Yap YJ, Ling TC, Chang JS, Show PL
    Ultrason Sonochem, 2018 Nov;48:231-239.
    PMID: 30080546 DOI: 10.1016/j.ultsonch.2018.06.002
    In this study, a simple sugaring-out supported by liquid biphasic flotation technique combined with ultrasonication was introduced for the extraction of proteins from microalgae. Sugaring-out as a phase separation method is novel and has been used in the extraction of metal ions, biomolecules and drugs. But, its functioning in protein separation from microalgae is still unknown. In this work, the feasibility of sugaring-out coupled with ultrasound for the extraction of protein was investigated. Primary studies were carried out to examine the effect of sonication on the microalgae cell as well as the separation efficiency of the integrated method. Effect of various operating parameters such as the concentration of microalgae biomass, the location of sonication probe, sonication time, ultrasonic pulse mode (includes varying ON and OFF duration of sonication), concentration of glucose, types of sugar, concentration of acetonitrile and the flow rate in the flotation system for achieving a higher separation efficiency and yield of protein were assessed. Besides, a large-scale study of the integration method was conducted to verify the consistency of the followed technique. A maximum efficiency (86.38%) and yield (93.33%) were attained at the following optimized conditions: 0.6% biomass concentration, 200 g/L of glucose concentration, 100% acetonitrile concentration with 5 min of 5 s ON/10 s OFF pulse mode and at a flow rate of 100 cc/min. The results obtained for large scale were 85.25% and 92.24% for efficiency and yield respectively. The proposed liquid biphasic flotation assisted with ultrasound for protein separation employing sugaring-out demonstrates a high production and separation efficiency and is a cost-effective solution. More importantly, this method provides the possibility of extending its application for the extraction of other important biomolecules.
    Matched MeSH terms: Plant Proteins/isolation & purification*
  3. Anesthetic Molecule Interaction of Noble Gases with Proteins and Lipids and their Effect: A Review
    Kundu SK, Chakraborty C, Yagihara S, Teoh SL, Das S
    Curr Drug Deliv, 2018;15(10):1381-1392.
    PMID: 30124152 DOI: 10.2174/1567201815666180820101255
    Surgical operations are impossible without administering proper analgesia. Advancement in the field of anesthesia has invariably resulted in the accomplishment of all surgical processes without any inconvenience. Admittedly, the use of noble gas is on the decline. The noble gases may not interact chemically with any other substance under normal temperature and pressure but they may interact with proteins and lipids. Different anesthetic molecules may stimulate either proteins or lipids in membrane. There is a connection between the anesthetic molecules and the hydrophobic region of the membrane. In the present review, we attempt to highlight the interaction between the anesthetic molecule with proteins and lipids and their effects. We sketched few noble gases and some other existing molecules such as halothane and alcohol which interacted with proteins and lipids.
    Matched MeSH terms: Proteins/chemistry*
  4. Fulltext Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, Calloselasma rhodostoma (Malayan Pit Viper) and Ophiophagus hannah (King Cobra)
    Kunalan S, Othman I, Syed Hassan S, Hodgson WC
    Toxins (Basel), 2018 Oct 26;10(11).
    PMID: 30373186 DOI: 10.3390/toxins10110434
    Calloselasma rhodostoma (CR) and Ophiophagus hannah (OH) are two medically important snakes found in Malaysia. While some studies have described the biological properties of these venoms, feeding and environmental conditions also influence the concentration and distribution of snake venom toxins, resulting in variations in venom composition. Therefore, a combined proteomic approach using shotgun and gel filtration chromatography, analyzed by tandem mass spectrometry, was used to examine the composition of venoms from these Malaysian snakes. The analysis revealed 114 proteins (15 toxin families) and 176 proteins (20 toxin families) in Malaysian Calloselasma rhodostoma and Ophiophagus hannah species, respectively. Flavin monoamine oxidase, phospholipase A₂, phosphodiesterase, snake venom metalloproteinase, and serine protease toxin families were identified in both venoms. Aminopeptidase, glutaminyl-peptide cyclotransferase along with ankyrin repeats were identified for the first time in CR venom, and insulin, c-type lectins/snaclecs, hepatocyte growth factor, and macrophage colony-stimulating factor together with tumor necrosis factor were identified in OH venom for the first time. Our combined proteomic approach has identified a comprehensive arsenal of toxins in CR and OH venoms. These data may be utilized for improved antivenom production, understanding pathological effects of envenoming, and the discovery of biologically active peptides with medical and/or biotechnological value.
    Matched MeSH terms: Reptilian Proteins/analysis*
  5. Proteomics in Systems Biology
    Aizat WM, Hassan M
    Adv Exp Med Biol, 2018 11 2;1102:31-49.
    PMID: 30382567 DOI: 10.1007/978-3-319-98758-3_3
    Proteomics is the study of proteins, the workhorses of cells. Proteins can be subjected to various post-translational modifications, making them dynamic to external perturbation. Proteomics can be divided into four areas: sequence, structural, functional and interaction and expression proteomics. These different areas used different instrumentations and have different focuses. For example, sequence and structural proteomics mainly focus on elucidating a particular protein sequence and structure, respectively. Meanwhile, functional and interaction proteomics concentrate on protein function and interaction partners, whereas expression proteomics allows the cataloguing of total proteins in any given samples, hence providing a holistic overview of various proteins in a cell. The application of expression proteomics in cancer and crop research is detailed in this chapter. The general workflow of expression proteomics consisting the use of mass spectrometry instrumentation has also been described, and some examples of proteomics studies are also presented.
    Matched MeSH terms: Proteins/chemistry*
  6. Fulltext Online shopping practices and factors influencing
    Farhan Mat Arisah, Shamsul Azahari Zainal Badari, Ahmad Hariza Hashim
    Malaysian Journal of Social Sciences and Humanities, 2016;1(3):111-123.
    MyJurnal
    Online shopping has been preferred over the conventional shopping methods as the results of hectic
    lifestyles nowadays. It is a convenient way to get groceries without having to queue at the counters.
    The objective of this research is to study the online shopping practices and the factors that affect it.
    Apart from that, it is also to identify the difference in online shopping behaviour among selected
    background factors (sex, social status, generation, ethnic, household income, education level and zone).
    The populations of this research include consumers of 17 years old and above whose chosen by
    convenience through mall intercept method. T-test result shows there is significant difference (t=-
    3.156, p=0.001) for online shopping practice between men and women. Meanwhile, based on ANOVA result, it shows that there is significant difference between mean scores of different generation [F(3,
    1298)= 3.475, p = 0.016], ethnics [F(4, 1291)= 3.678, p = 0.006] and household income [F (4, 1227)=
    6.935, p = 0.000]. However, there is no significant difference among mean scores for education level,
    zones and stratum (p>0.05). Apart from that, the result also indicates that only five factors taken into
    consideration before a particular customer does the online shopping which are safety, products offered,
    risk, trustworthy and customers experience. Thus, the sellers should approach their customers in
    different ways in order to meet the demand, hence expanding their bussiness.
    Matched MeSH terms: Nerve Tissue Proteins; RNA-Binding Proteins
  7. Fulltext Microleakage evaluation in teeth restored with experimental dental nanocomposite
    Rana K. Al-Bayati, Rao, Gururajaprasad Kaggal Lakshmana, Dasmawati Mohamad, Azman Seeni, Norehan Mokhtar
    Malaysian Journal of Medicine and Health Sciences, 2018;14(102):1-6.
    MyJurnal
    The aim of this study was to evaluate the experimental nanocomposite (Kelfil) against microleakage when bonded with two different types of bonding systems. Methods: Sixty sound extracted human permanent incisors were divided into six groups randomly. Each tooth was prepared with standardised Class III cavity on each proximal surface. The teeth were immersed in Rhodamin B dye for ten hours and sectioned to analyse the depth of dye penetration. The depth of the dye penetration was measured in micrometres by using confocal laser scanning microscope (CLSM). Results: The depth of the dye penetration in the groups was compared by ANOVA test. The results showed comparable microleakage between different types of composites. Conclusion: The experimental nanocomposite (Kelfil) in comparison to the commercially available nanocomposite and micro-hybrid composite has comparable microleakage when bonded using self-etching and total-etched adhesive systems.
    Matched MeSH terms: Nerve Tissue Proteins; RNA-Binding Proteins
  8. Perbezaan kecemerlangan pelajar dari perspektif pengurusan masa, keputusan akademik terdahulu dan jantina
    Hamdan A, Nasir R, Khairudin R, Wan Shahrazad WS
    Jurnal Psikologi Malaysia, 2015;olume 29:39-51.
    The purpose of this study was to examine and identify whether factors such as previous academic result (SPM), gender and time management could determine differences among students, based on academic performance or grade point average (GPA). A total of 551 undergraduate students from four universities participated in the study. Students with a GPA of 3.50 or greater significantly differed in their level of time management than those with a GPA of less than 2.50. In terms of gender, female students were found to have higher GPA than male students. However, students enrolled with excellent SPM result did not differ significantly in term of their GPA from students enrolled with low SPM result. Results from ANOVA and t-test showed that time management and gender, not previous academic result, were the important factors to distinguish students with low or high academic performance. The implication of this study showed that academic excellence among undergraduates did not rely solely on previous academic result, but the role of time management and gender did play significant factors in distinguishing high achievers vs low achievers.
    Matched MeSH terms: Nerve Tissue Proteins; RNA-Binding Proteins
  9. Soil properties and variation between three forest types in tropical watershed forest of Chini Lake, Peninsular Malaysia
    Khairil M, Wan Juliana WA, Nizam M, Razi Idris W
    Sains Malaysiana, 2014;43:1635-1643.
    Three forest types were recognized at Chini watershed namely inland, seasonal flood and riverine forests. The soil physico-chemical characteristics from the three forest types were investigated to determine the soil properties variation within a landscape scale. Thirty sampling stations were established, represented by fourteen inland, nine stations in seasonal flood forest and seven in riverine forest. In each station, three soil samples were taken at 0-15 cm depth by using an auger. The study showed 71% of the soil in the inland forest was found to be dominated by clay, 44% of the soil in the seasonal flood forest by clay loam and 42% of the soil in the riverine forest was dominated by silty clay. The pH of all three types of forest studied was acidic and insignificantly different. Organic matter content in the study sites was moderate. The mean of electric conductivity (EC) and cation exchange capacity (CEC) values in the studied soils were low. Based on ANOVA, there were significant differences of the available P and K, K+, Ca2+ and Mg2+ cations and electrical conductivity amongst the three forest types (p<0.05). Cluster analysis showed that the variations of the soil physico-chemical characteristics between the three forest types were low thus indicating that the soil physico-chemical investigated in this study were not the only main contributing factors in floristic variation of the three forest types in Chini watershed.
    Matched MeSH terms: Nerve Tissue Proteins; RNA-Binding Proteins
  10. Penagihan heroin: Implikasi kepada saraf otak, ingatan serta pengaruhnya terhadap fenomena tingkah laku relaps dengan menggunakan FMRI
    Chandralekah SV, Wan Shahrazad WS, Khairudin R, Syazarina SO, Mahamood Y
    Jurnal Psikologi Malaysia, 2013;olume 27:55-71.
    Kes relaps dalam kalangan penagih dadah heroin semakin meningkat bilangannya di Malaysia. Kajian ini cuba mengaitkan kes relaps ini dengan tempoh penyalahgunaan heroin terhadap daya ingatan dan bahagian otak dalam usaha meleraikan persoalan tingkah laku relaps. Seramai 45 orang subjek terlibat dalam kajian ini. Kesemua subjek dipilih secara rawak berdasarkan kepada tempoh penagihan. Subjek dibahagi berdasarkan tempoh penagihan singkat, penagihan lama dan subjek normal. Setiap kumpulan diwakili seramai 15 orang subjek. Kajian ini dijalankan secara eksperimen selari dengan menggunakan ujian tingkah laku melalui ujian ingatan N-Back dan juga ujian pengimejan melalui ujian (fMRI). Dapatan data daripada ujian tingkah laku dianalisis menggunakan Program SPSS. Manakala data daripada ujian kefungsian pengimejan resonans magnet (fMRI) dianalisis menggunakan perisian pemetaan statistik berparameter (SPM) dan MATLAB (r2008a). Pengujian hipotesis dilakukan dengan menggunakan ujian ANOVA rekabentuk campuran (repeated measure) dan juga Post Hoc serta analisis kesan rawak (RFX) untuk melihat tahap pengaktifan otak yang dilakukan dengan menggunakan SPM. Hasil kajian menunjukkan bahawa daya ingatan berbeza secara signifikan mengikut tempoh pengambilan, wujud interaksi yang signifikan antara tempoh pengambilan dadah dengan ujian N-Back, dan perbezaan corak pengaktifan pada bahagian otak dalam kumpulan eksperimen dan kawalan mengikut kesukaran ujian N-Back yang diberikan. Kajian ini menunjukkan semakin tinggi tempoh penagihan semakin kurang pengaktifan bahagian otak dan semakin tinggilah kecenderungan penagih untuk merelaps.
    Matched MeSH terms: Nerve Tissue Proteins; RNA-Binding Proteins
  11. The effect of food safety education on handwashing practices in school canteens’ food handlers
    Nik Rosmawati N, Wan Manan W, Noor Izani N, Nik Nurain N, Razlina A
    Sains Malaysiana, 2018;47:2119-2128.
    This study aims to assess the effectiveness of a newly developed food safety education to improve the handwashing
    practices of food handlers in school canteens. A community-based intervention study was carried out over a 2-year period.
    Sixteen out of 98 primary schools were randomly selected and assigned into intervention and control groups using a
    simple random sampling method. The study population included food handlers who worked in the canteens of the school
    selected. The Food Safety Education Programme (FSEP) for the intervention group was developed based on the theory of
    planned behaviour. The main outcome measures used were handwashing practices from the observations carried out at
    baseline, 6-weeks (Post1) and 12-weeks (Post2) after the intervention. Out of 79 food handlers who participated in this
    study, 33 (41.8%) were in the intervention group and 46 (58.2%) were in the control group. Prior to FSEP, handwashing
    was not commonly practiced following critical events and the majority did not perform correctly. The time-effect of the
    mixed design analysis of variance showed a significant increase (p=0.004) in the mean percentage of the total observed
    handwashing practices from 29% at the baseline to 50.8% at Post1 (p=0.004). However, the intervention-effect of mixed
    design ANOVA did not show any significant difference in the handwashing practices (p=0.210). The FSEP was effective in
    improving the handwashing practices of the food handlers in the selected primary school canteens.
    Matched MeSH terms: Nerve Tissue Proteins; RNA-Binding Proteins
  12. Effects of Monascus application on in vitro digestion and fermentation characteristics of fish protein
    Liu J, Chen J, Wang S, Xie J, Wang Y, Chai TT, et al.
    Food Chem, 2022 May 30;377:132000.
    PMID: 34999460 DOI: 10.1016/j.foodchem.2021.132000
    The aim of this study was to investigate the digestion and fermentation properties of fish protein fermented by Monascus. Semi-dried fish was fermented by applying Monascus purpureus Went M 3.439. Our results show that the Monascus fermentation of the fish protein enriched the free amino acids and achieved a relatively higher glutamate content than the control group. The Monascus treatment promoted the decomposition of the fish protein during in vitro digestion, reduced the ammonia and indole content and tended to increase the propionic acid content during in vitro fermentation. The Monascus treatment considerably changed the gut microbiota composition, and particularly increased the relative abundance of Parabacteroides in the in vitro fermentation model of human distal colon. Consumption of Monascus fermented fish protein could result in positive changes in fermentation metabolites and gut microbiota, which brings potential health benefits.
    Matched MeSH terms: Fish Proteins/metabolism
  13. Association between autophagy and KRAS mutation with clinicopathological variables in colorectal cancer patients
    Awi NJ, Yap HY, Armon S, Low JSH, Peh KB, Peh SC, et al.
    Malays J Pathol, 2021 Aug;43(2):269-279.
    PMID: 34448791
    Autophagy is a host defensive mechanism responsible for eliminating harmful cellular components through lysosomal degradation. Autophagy has been known to either promote or suppress various cancers including colorectal cancer (CRC). KRAS mutation serves as an important predictive marker for epidermal growth factor receptor (EGFR)-targeted therapies in CRC. However, the relationship between autophagy and KRAS mutation in CRC is not well-studied. In this single-centre study, 92 formalin-fixed paraffin-embedded (FFPE) tissues of CRC patients (42 Malaysian Chinese and 50 Indonesian) were collected and KRAS mutational status was determined by quantitative PCR (qPCR) (n=92) while the expression of autophagy effector (p62, LC3A and LC3B) was examined by immunohistochemistry (IHC) (n=48). The outcomes of each were then associated with the clinicopathological variables (n=48). Our findings demonstrated that the female CRC patients have a higher tendency in developing KRAS mutation in the Malaysian Chinese population (p<0.05). Expression of autophagy effector LC3A was highly associated with the tumour grade in CRC (p<0.001) but not with other clinicopathological parameters. Lastly, the survival analysis did not yield a statistically significant outcome. Overall, this small cohort study concluded that KRAS mutation and autophagy effectors are not good prognostic markers for CRC patients.
  14. Proteomic Characterization and Comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus Venom Using Shotgun-Proteomics
    Zainal Abidin SA, Rajadurai P, Chowdhury ME, Ahmad Rusmili MR, Othman I, Naidu R
    Toxins (Basel), 2016 10 18;8(10).
    PMID: 27763534
    Tropidolaemus wagleri and Cryptelytrops purpureomaculatus are venomous pit viper species commonly found in Malaysia. Tandem mass spectrometry analysis of the crude venoms has detected different proteins in T. wagleri and C. purpureomaculatus. They were classified into 13 venom protein families consisting of enzymatic and nonenzymatic proteins. Enzymatic families detected in T. wagleri and C. purpureomaculatus venom were snake venom metalloproteinase, phospholipase A₂, ʟ-amino acid oxidase, serine proteases, 5'-nucleotidase, phosphodiesterase, and phospholipase B. In addition, glutaminyl cyclotransferase was detected in C. purpureomaculatus. C-type lectin-like proteins were common nonenzymatic components in both species. Waglerin was present and unique to T. wagleri-it was not in C. purpureomaculatus venom. In contrast, cysteine-rich secretory protein, bradykinin-potentiating peptide, and C-type natriuretic peptide were present in C. purpureomaculatus venom. Composition of the venom proteome of T. wagleri and C. purpureomaculatus provides useful information to guide production of effective antivenom and identification of proteins with potential therapeutic applications.
    Matched MeSH terms: Reptilian Proteins/analysis*
  15. Benchmarking Diet Quality to Assess Nutritional Risk in Hemodialysis Patients: Applying Adequacy and Moderation Metrics of the Hemodialysis-Healthy Eating Index
    Sualeheen A, Khor BH, Balasubramanian GV, Sahathevan S, Chinna K, Mat Daud ZA, et al.
    J Ren Nutr, 2022 Nov;32(6):726-738.
    PMID: 35182714 DOI: 10.1053/j.jrn.2022.02.002
    OBJECTIVES: This study modified Healthy Eating Index (HEI) based on hemodialysis (HD)-specific nutritional guidelines and investigated associations between the diet quality (DQ) and nutritional risk in HD patients.

    METHODS: The HD-HEI tool adapted the Malaysian Dietary Guidelines 2010 framework according to HD-specific nutrition guidelines. This HD-HEI was applied to 3-day dietary records of 382 HD patients. Relationships between HD-HEI scores and nutritional parameters were tested by partial correlations. Binary logistic regression models adjusted with confounders were used to determine adjusted odds ratio (adjOR) with 95% confidence interval (CI) for nutritional risk based on HD-HEI scores categorization.

    RESULTS: The total HD-HEI score (51.3 ± 10.2) for this HD patient population was affected by ethnicity (Ptrend < .001) and sex (P = .003). No patient achieved "good" DQ (score: 81-100), while DQ of 54.5% patients were classified as "needs improvement" (score: 51-80) and remaining as "poor" (score: 0-51). Total HD-HEI scores were positively associated with dietary energy intake (DEI), dietary protein intake (DPI), dry weight, and handgrip strength, but inversely associated with Dietary Monotony Index (DMI) (all P 

    Matched MeSH terms: Dietary Proteins*
  16. Fulltext Genetic Diversity and Clustering of the Rhoptry Associated Protein-1 of Plasmodium knowlesi from Peninsular Malaysia and Malaysian Borneo
    Azlan UW, Lau YL, Fong MY
    Korean J Parasitol, 2022 Dec;60(6):393-400.
    PMID: 36588415 DOI: 10.3347/kjp.2022.60.6.393
    Human infection with simian malaria Plasmodium knowlesi is a cause for concern in Southeast Asian countries, especially in Malaysia. A previous study on Peninsular Malaysia P. knowlesi rhoptry associated protein-1 (PkRAP1) gene has discovered the existence of dimorphism. In this study, genetic analysis of PkRAP1 in a larger number of P. knowlesi samples from Malaysian Borneo was conducted. The PkRAP1 of these P. knowlesi isolates was PCR-amplified and sequenced. The newly obtained PkRAP1 gene sequences (n = 34) were combined with those from the previous study (n = 26) and analysed for polymorphism and natural selection. Sequence analysis revealed a higher genetic diversity of PkRAP1 compared to the previous study. Exon II of the gene had higher diversity (π = 0.0172) than exon I (π = 0.0128). The diversity of the total coding region (π = 0.0167) was much higher than those of RAP1 orthologues such as PfRAP-1 (π = 0.0041) and PvRAP1 (π = 0.00088). Z-test results indicated that the gene was under purifying selection. Phylogenetic tree and haplotype network showed distinct clustering of Peninsular Malaysia and Malaysian Borneo PkRAP1 haplotypes. This geographical-based clustering of PkRAP1 haplotypes provides further evidence of the dimorphism of the gene and possible existence of 2 distinct P. knowlesi lineages in Malaysia.
    Matched MeSH terms: Protozoan Proteins/genetics
  17. Generation of an RNA aptamer against LipL32 of Leptospira isolated by Tripartite-hybrid SELEX coupled with in-house Python-aided unbiased data sorting
    Shien Yeoh T, Yusof Hazrina H, Bukari BA, Tang TH, Citartan M
    Bioorg Med Chem, 2023 Mar 01;81:117186.
    PMID: 36812779 DOI: 10.1016/j.bmc.2023.117186
    Leptospirosis is a potentially life-threatening zoonosis caused by pathogenic Leptospira. The major hurdle of the diagnosis of Leptospirosis lies in the issues associated with current methods of detection, which are time-consuming, tedious and the need for sophisticated, special equipments. Restrategizing the diagnostics of Leptospirosis may involve considerations of the direct detection of the outer membrane protein, which can be faster, cost-saving and require fewer equipments. One such promising marker is LipL32, which is an antigen with high amino acid sequence conservation among all the pathogenic strains. In this study, we endeavored to isolate an aptamer against LipL32 protein via a modified SELEX strategy known as tripartite-hybrid SELEX, based on 3 different partitioning strategies. In this study, we also demonstrated the deconvolution of the candidate aptamers by using in-house Python-aided unbiased data sorting in examining multiple parameters to isolate potent aptamers. We have successfully generated an RNA aptamer against LipL32 of Leptospira, LepRapt-11, which is applicable in a simple direct ELASA for the detection of LipL32. LepRapt-11 can be a promising molecular recognition element for the diagnosis of leptospirosis by targeting LipL32.
    Matched MeSH terms: Bacterial Outer Membrane Proteins/metabolism
  18. Complete genome sequence of Bacillus Licheniformis NWMCC0046, a candidate for the laundry industry
    Zhou W, Zeng S, Yu J, Xiang J, Zhang F, Takriff MS, et al.
    J Basic Microbiol, 2023 Feb;63(2):223-234.
    PMID: 36538731 DOI: 10.1002/jobm.202200528
    In this study, selected properties of protease and the complete genome sequence of Bacillus licheniformis NWMCC0046 were investigated, to discover laundry applications and other potential probiotic properties of this strain. Partial characterization of B. licheniformis NWMCC0046 showed that its protease has good activity both in alkaline environments and at low temperatures. Also, the protease is compatible with commercial detergents and can be used as a detergent additive for effective stain removal at low temperatures. The complete genome sequence of B. licheniformis NWMCC0046 is comprised of a 4,321,565 bp linear chromosome with a G + C content of 46.78% and no plasmids. It had 4504 protein-encoding genes, 81 transfer RNA (tRNA) genes, and 24 ribosomal RNA (rRNA) genes. Genomic analysis revealed genes involved in exocellular enzyme production and probiotic properties. In addition, genomic sequence analysis revealed specific genes encoding carbohydrate metabolism pathways, resistance, and cold adaptation capacity. Overall, protease properties show its potential as a detergent additive enzyme. The complete genome sequence information of B. licheniformis NWMCC0046 was obtained, and functional prediction revealed its numerous probiotic properties.
    Matched MeSH terms: Bacterial Proteins/metabolism
  19. In silico analysis prediction of HepTH1-5 as a potential therapeutic agent by targeting tumour suppressor protein networks
    Azemin WA, Alias N, Ali AM, Shamsir MS
    J Biomol Struct Dyn, 2023 Mar;41(4):1141-1167.
    PMID: 34935583 DOI: 10.1080/07391102.2021.2017349
    Many studies reported that the activation of tumour suppressor protein, p53 induced the human hepcidin expression. However, its expression decreased when p53 was silenced in human hepatoma cells. Contrary to Tilapia hepcidin TH1-5, HepTH1-5 was previously reported to trigger the p53 activation through the molecular docking approach. The INhibitor of Growth (ING) family members are also shown to directly interact with p53 and promote cell cycle arrest, senescence, apoptosis and participate in DNA replication and DNA damage responses to suppress the tumour initiation and progression. However, the interrelation between INGs and HepTH1-5 remains unknown. Therefore, this study aims to identify the mechanism and their protein interactions using in silico approaches. The finding revealed that HepTH1-5 and its ligands had interacted mostly on hotspot residues of ING proteins which involved in histone modifications via acetylation, phosphorylation, and methylation. This proves that HepTH1-5 might implicate in an apoptosis signalling pathway and preserve the protein structure and function of INGs by reducing the perturbation of histone binding upon oxidative stress response. This study would provide theoretical guidance for the design and experimental studies to decipher the role of HepTH1-5 as a potential therapeutic agent for cancer therapy. Communicated by Ramaswamy H. Sarma.
    Matched MeSH terms: Tumor Suppressor Proteins/genetics
  20. LYRM7-associated mitochondrial complex III deficiency with non-cavitating leukoencephalopathy and stroke-like episodes
    Alfattal R, Alfarhan M, Algaith AM, Albash B, Elshafie RM, Alshammari A, et al.
    Am J Med Genet A, 2023 May;191(5):1401-1411.
    PMID: 36757047 DOI: 10.1002/ajmg.a.63143
    Defects of respiratory chain complex III (CIII) result in characteristic but rare mitochondrial disorders associated with distinct neuroradiological findings. The underlying molecular defects affecting mitochondrial CIII assembly factors are few and yet to be identified. LYRM7 assembly factor is required for proper CIII assembly where it acts as a chaperone for the Rieske iron-sulfur (UQCRFS1) protein in the mitochondrial matrix and stabilizing it. We present here the seventeenth individual with LYRM7-associated mitochondrial leukoencephalopathy harboring a previously reported rare pathogenic homozygous LYRM 7 variant, c.2T>C, (p.Met1?). Like previously reported individuals, our 5-year-old male proband presented with recurrent metabolic and lactic acidosis, encephalopathy, and fatigue. Further, he has additional, previously unreported features, including an acute stroke like episode with bilateral central blindness and optic neuropathy, recurrent hyperglycemia and hypertension associated with metabolic crisis. However, he has no signs of psychomotor regression. He has been stable clinically with residual left-sided reduced visual acuity and amblyopia, and no more metabolic crises for 2-year-period while on the mitochondrial cocktail. Although the reported brain MRI findings in other affected individuals are homogenous, it is slightly different in our index, revealing evidence of bilateral almost symmetric multifocal periventricular T2 hyperintensities with hyperintensities of the optic nerves, optic chiasm, and corona radiata but with no cavitation or cystic changes. This report describes new clinical and radiological findings of LYRM7-associated disease. The report also summarizes the clinical and molecular data of previously reported individuals describing the full phenotypic spectrum.
    Matched MeSH terms: Mitochondrial Proteins/genetics
Related Terms
Filters
Contact Us

Please provide feedback to Administrator ([email protected])

External Links