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DNA barcoding and phylogenetic analysis of Malaysian groupers (Subfamily: Epinephelinae) using mitochondrial Cytochrome c oxidase I (COI) gene

Aziz NMA, Esa Y, Arshad A

J Environ Biol, 2016 07;37(4 Spec No):725-33.
PMID: 28779732

The present study was carried out to examine the species identification and phylogenetic relationships of groupers in Malaysia using mitochondrial Cytochrome c Oxidase I (COI) gene, commonly known as barcoding gene. A total of 63 individuals comprising 10 species from three genera were collected from the coastal areas of Johor, Kelantan, Pahang, Perak, Selangor and Terengganu. All the individuals were morphologically identified and molecular works involved polymerase chain reaction (PCR) and sequencing of COI barcoding fragment (655 base pairs). Results from the BLAST search showed that 55 sequences could be assigned to 10 grouper species with high percentage identity index (≥95% to 100%), while eight grouper individuals showed discrepancies in their taxonomic identification based on the morphology and the COI barcoding results. The histogram of distances showed that there was a clear-cut barcode gap present in the sequences indicating a clear separation between intraspecific and interspecific distances. The pairwise genetic distances showed lowest pairwise distance between P. leopardus and P. maculatus (4.4%), while the highest pairwise distance was between E. bleekeri and P. maculatus (23.5%), supporting their morphological and habitat similarities and differences. Phylogenetic analysis (Neighbor-Joining) showed the presence of two major clades (1) genus Epinephelus vs (2) genus Plectropomus and Cephalopholis). In conclusion, the present study has managed to show the accuracy of DNA barcoding method for species identification, and utilization of COI gene for phylogenetic study among groupers. ?
Seasonal variations of zooplankton biomass and size-fractionated abundance in relation to environmental changes in a tropical mangrove estuary in the Straits of Malacca

Balqis ARS, Yusoff FM, Arshad A, Nishikawa J

J Environ Biol, 2016 Jul;37(4 Spec No):685-95.
PMID: 28779727

Seasonal variations of zooplankton community in terms of biomass and size-fractionated densities were studied in a tropical Sangga Kechil river, Matang, Perak from June 2010 to April 2011. Zooplankton and jellyfish (hydromedusae, siphonophores and ctenophores) samples were collected bimonthly from four sampling stations by horizontal towing of a 140-?m plankton net and 500 ?m bongo net, respectively. A total of 12 zooplankton groups consisting of six groups each of mesozooplankon (0.2 mm-2.0 mm) and macrozooplankton (2.0 mm-20.0 cm) were recorded. The total zooplankton density (12375?3339 ind m(-3)) and biomass (35.32?14.56 mg m(-3)) were highest during the northeast (NE) monsoon and southwest (SW) monsoon, respectively, indicating the presence of bigger individuals in the latter season. Mesozooplankton predominated (94%) over the macrozooplankton (6%) during all the seasons, and copepods contributed 84% of the total mesozooplankton abundance. Macrozooplankton was dominated by appendicularians during most of the seasons (43%-97%), except during the NE monsoon (December) when chaetognaths became the most abundant (89% of the total macrozooplankton). BIO-ENV analysis showed that total zooplankton density was correlated with turbidity, total nitrogen and total phosphorus, which in turn was positively correlated to chlorophyll a. Cluster analysis of the zooplankton community showed no significant temporal difference between the SW and NE monsoon season during the study period (> 90% similarity). The present study revealed that the zooplankton community in the tropical mangrove estuary in the Straits of Malacca was dominated by mesoplankton, especially copepods.
Comprehensive review of Argulus infestations in aquaculture: Biological impacts and advanced management strategies

Haridevamuthu B, Raj D, Arshad A, Arockiaraj J

Fish Shellfish Immunol, 2024 Aug 20.
PMID: 39173980 DOI: 10.1016/j.fsi.2024.109851

The aquaculture industry is hindered by various factors. One of the most noticeable factors is infection by parasites and pathogens. Argulus stands out as a prominent and economically significant ectoparasite in freshwater aquaculture. Argulus infestation causes severe immunomodulatory effects on its hosts by promoting argulosis, causing inflammation, extensive tissue damage, and death. Indian aquaculture sector faced a loss of 62.5 million USD due to Argulus infection. However, current control methods, such as pesticides, cause serious environmental damage. Herbal treatment methods are ineffective and have limitations. Hence, a more efficient and cost-effective control method is needed. In recent years, vaccine development has emerged as a promising avenue of research. Understanding the effect of the host-parasite relationship in the host immune system is essential to develop strategies for prevention, control, and management of argulosis. These interactions provide insights into the co-evolutionary dynamics between hosts and parasites. This review provides an overview of the current knowledge on the host-searching behaviour of Argulus, host-parasite interaction and control strategies. This review also highlights the need for further research and the development of sustainable control measures for Argulus infection.
Fulltext Ethosomal nanocarriers: the impact of constituents and formulation techniques on ethosomal properties, in vivo studies, and clinical trials

Abdulbaqi IM, Darwis Y, Khan NA, Assi RA, Khan AA

Int J Nanomedicine, 2016;11:2279-304.
PMID: 27307730 DOI: 10.2147/IJN.S105016

Ethosomal systems are novel lipid vesicular carriers containing a relatively high percentage of ethanol. These nanocarriers are especially designed for the efficient delivery of therapeutic agents with different physicochemical properties into deep skin layers and across the skin. Ethosomes have undergone extensive research since they were invented in 1996; new compounds were added to their initial formula, which led to the production of new types of ethosomal systems. Different preparation techniques are used in the preparation of these novel carriers. For ease of application and stability, ethosomal dispersions are incorporated into gels, patches, and creams. Highly diverse in vivo models are used to evaluate their efficacy in dermal/transdermal delivery, in addition to clinical trials. This article provides a detailed review of the ethosomal systems and categorizes them on the basis of their constituents to classical ethosomes, binary ethosomes, and transethosomes. The differences among these systems are discussed from several perspectives, including the formulation, size, ζ-potential (zeta potential), entrapment efficiency, skin-permeation properties, and stability. This paper gives a detailed review on the effects of ethosomal system constituents, preparation methods, and their significant roles in determining the final properties of these nanocarriers. Furthermore, the novel pharmaceutical dosage forms of ethosomal gels, patches, and creams are highlighted. The article also provides detailed information regarding the in vivo studies and clinical trials conducted for the evaluation of these vesicular systems.
Fulltext Griscelli syndrome

Ariffin H, Geikowski A, Chin TF, Chau D, Arshad A, Abu Bakar K, et al.

Med J Malaysia, 2014 Aug;69(4):193-4.
PMID: 25500851 MyJurnal

We report a case of Griscelli Syndrome (GS). Our patient initially presented with a diagnosis of haemophagocytic lymphistiocytosis (HLH). Subsequent microscopic analysis of the patient's hair follicle revealed abnormal distribution of melanosomes in the shaft, which is a hallmark for GS. Analysis of RAB27A gene in this patient revealed a homozygous mutation in exon 6, c.550C>T, p.R184X . This nonsense mutation causes premature truncation of the protein resulting in a dysfunctional RAB27A. Recognition of GS allows appropriate institution of therapy namely chemotherapy for HLH and curative haemotopoeitic stem cell transplantation.
Description and evaluation of imposex in Strombus canarium Linnaeus, 1758 (Gastropoda, Strombidae): a potential bio-indicator of tributyltin pollution

Cob ZC, Arshad A, Bujang JS, Abd Ghaffar M

Environ Monit Assess, 2011 Jul;178(1-4):393-400.
PMID: 20824325 DOI: 10.1007/s10661-010-1698-7

Strombus canarium Linnaeus, 1758 is an important gastropod species within the study area and was traditionally collected for food by the locals. The objective of the present study is to assess the incidence of imposex and its severity in this species. Adult conchs were sampled during their main reproductive period, from October 2005 to January 2006, at Sungai Pulai estuary, Johor Straits, Malaysia. A total of 32.81% of adult females showed imposex characteristics, with varying degrees of severity though. The relative penis size (RPS) index ranged from 1.74 to 33.29 (mean = 13.40 ± 2.27, n = 21), while the relative penis length (RPL) index ranged from 6.28 to 55.19 (mean = 25.83 ± 3.33, n = 21). The use of vas deferens sequence (VDS) index was however cannot be applied as the presence of egg groove obscured any vas deferens development in affected females. Sequence of imposex (male penis) development in female conch, from merely a small stump to an advance male penis homologous was therefore carefully analyzed and described, and an alternative imposex classification scheme was proposed. S. canarium can be a good indicator for monitoring of organotin pollution within the study area. However, more studies are needed in order to further develop and test its validity and application, such as its correlation with levels of pollutant within the tissues and the environment, as well as its application on other Strombus species.
Anthropogenic impacts on heavy metal concentrations in the coastal sediments of Dumai, Indonesia

Amin B, Ismail A, Arshad A, Yap CK, Kamarudin MS

Environ Monit Assess, 2009 Jan;148(1-4):291-305.
PMID: 18274874 DOI: 10.1007/s10661-008-0159-z

Concentrations of Cd, Cu, Pb, Zn, Ni and Fe were determined in the surface sediments to investigate the distributions, concentrations and the pollution status of heavy metals in Dumai coastal waters. Sediment samples from 23 stations, representing 5 different site groups of eastern, central and western Dumai and southern and northern Rupat Island, were collected in May 2005. The results showed that heavy metal concentrations (in microg/g dry weight; Fe in %) were 0.88 (0.46-1.89); 6.08 (1.61-13.84); 32.34 (14.63-84.90); 53.89 (31.49-87.11); 11.48 (7.26-19.97) and 3.01 (2.10-3.92) for Cd, Cu, Pb, Zn, Ni and Fe, respectively. Generally, metal concentrations in the coastal sediments near Dumai city center (eastern and central Dumai) which have more anthropogenic activities were higher than those at other stations. Average concentration of Cd in the eastern Dumai was slightly higher than effective range low (ERL) but still below effective range medium (ERM) value established by Long et al. (Environmental Management 19(1):81-97, 1995; Environmental Toxicology Chemistry 17(4):714-727, 1997). All other metals were still below ERL and ERM. Calculated enrichment factor (EF), especially for Cd and Pb, and the Pollution load index (PLI) value in the eastern Dumai were also higher than other sites. Cd showed higher EF when compared to other metals. Geo-accumulation indices (I(geo)) in most of the stations (all site groups) were categorized as class 1 (unpolluted to moderately polluted environment) and only Cd in Cargo Port was in class 2 (moderately polluted). Heavy metal concentrations found in the present study were comparable to other regions of the world and based on the calculated indices it can be classified as unpolluted to moderately polluted coastal environment.
Effects of some environmental parameters on the reproduction and development of a tropical marine harpacticoid copepod Nitocra affinis f. californica Lang

Matias-Peralta H, Yusoff FM, Shariff M, Arshad A

Mar Pollut Bull, 2005;51(8-12):722-8.
PMID: 16291188

The effects of salinity, temperature, and light conditions on the reproduction and development of harpacticoid copepod, Nitocra affinis f. californica under controlled laboratory conditions were determined. Seven different salinity levels (5, 10, 15, 20, 25, 30, 35 ppt), four temperatures (20, 25, 30, 35 degrees C), three different light intensities (25, 56, 130 micromol m(-2) s(-1)) and photoperiods (24 h:0 h, 1 h:23 h, 12 h:12 h LD cycle) were employed in this study. The highest (p < 0.05) overall reproduction and fastest development time were achieved by copepods reared under 30-35 ppt salinity. The optimum temperature required for the maximum reproduction was 30 degrees C while under 30 degrees C and 35 degrees C the copepod development time was shortest (p < 0.05) compared to other temperature levels. The overall reproduction was highest (p < 0.05) and development rate of N. affinis was shortest (p < 0.05) under lowest light intensity (25 micromol m(-2) s(-1)). Continuous light (24 h:0 h LD) inhibited the egg production while, continuous darkness (1 h:23 h LD) and 12 h:12 h LD significantly favoured the overall reproductive activity of the female. Photoperiods 1 h:23 h and 12 h:12 h LD yielded highest total (p < 0.05) offspring female(-1) coupled with highest (p < 0.05) survival percentage. This study illustrated that although N. affinis can tolerate wide range of environmental conditions, prolonged exposure to subnormal environments affect its reproduction and development. This study showed that this species can be mass cultured for commercial purposes and has a potential to be used for toxicity studies due to its high reproductive performance fast development and a wide range of tolerance to environmental conditions.
Antibacterial activity of ovary extract from sea urchin Diadema setosum

Marimuthu K, Gunaselvam P, Aminur Rahman M, Xavier R, Arockiaraj J, Subramanian S, et al.

Eur Rev Med Pharmacol Sci, 2015 May;19(10):1895-9.
PMID: 26044237

Sea urchin gonad is considered as a highly prized delicacy in several countries. It is also rich in valuable bioactive compounds including polyunsaturated fatty acids (PUFAs) and β-carotene. This study was undertaken to examine the antimicrobial properties of the ovary extract from sea urchin Diadema setosum against selected Gram-negative and Gram-positive bacteria.
Fulltext Vasovagal reaction among whole blood donors in Hospital Pulau Pinang. A statistical-epidemiological study

Hasan I, Arshad A, Rahim NA, Soo PY

Asian J Transfus Sci, 2020 07 24;14(1):28-32.
PMID: 33162702 DOI: 10.4103/ajts.AJTS_111_17

CONTEXT: Whole blood donation is generally considered a safe procedure; however, a small percentage of donors could develop vasovagal reactions (VVRs) during or after completion of blood donation.
AIMS: This study was undertaken to establish the prevalence of VVR among whole blood donors in Hospital Pulau Pinang and to investigate factors that lead to its occurrence.
SETTINGS AND DESIGN: A cross-sectional study was conducted involving 27,890 whole blood donations in 2016.
SUBJECTS AND METHODS: For each donation, donor's demographic and blood donation-related information was extracted from the blood bank database.
STATISTICAL ANALYSIS USED: Qualitative data including age group, sex, race, frequency, and location of donation were analyzed using Chi-square tests, while blood pressure was analyzed using t-test.
RESULTS: Overall, 425 cases of VVRs were reported, resulting in a VVR rate of 1.5% (one event in every 65 donations). We found a statistically significant association (P < 0.05) between the occurrence of VVRs with the young age group, female gender, Indian race, first-time donor, lower predonation blood pressure, and donation performed in a mobile donation campaign. The most common vasovagal symptoms are lightheadedness (88%), followed by nausea (5.4%), muscle twitching (3.5%), vomiting (1.4%), loss of consciousness <30 s (1.4%), and paresthesia (0.2%).
CONCLUSIONS: The prevalence of VVRs among whole blood donors in Hospital Pulau Pinang appeared to be low. Our study reaffirms that blood donation is a relatively safe process, and the incidence of VVR can be further reduced by ensuring strict screening procedure before blood donation.
Fulltext LeHeR, a simple novel approach for difficult airway in non-trauma patients

Yezid NH, Poh K, Md Noor J, Arshad A

BMJ Case Rep, 2019 Aug 10;12(8).
PMID: 31401573 DOI: 10.1136/bcr-2019-230201

Managing the difficult airway presents a great challenge to anaesthesiologists and emergency physicians. Although there are many methods and scoring systems available to predict and anticipate difficult airway, the dictum in emergency airway is to always expect the unexpected. We have encountered a novel simple method of improving laryngoscopic view in difficult airway. We report four cases of difficult airway encountered in our district hospital from November 2017 to December 2018, in which intubation was performed using a simple manoeuvre called supine left head rotation (LeHeR). In all these cases, LeHeR manoeuvre has proven to be successful after more than a single attempt at intubation using various methods. The manoeuvre improves drastically the laryngoscopic view of Cormack-Lehane from 3B and 4 to 1 and 2.
Morphometric relations in the grey eel catfish Plotosus canius in the coastal waters of Port Dickson, Peninsular Malaysia

Usman BI, Amin SM, Arshad A, Kamarudin MS

J Environ Biol, 2016 Jul;37(4):573-8.
PMID: 27498503

Samples of grey eel catfish Plotosus canius were collected from the coastal waters of Port Dickson, Malaysia from January to December, 2012. A total of 341 specimens (172 males and 169 females) were used to estimate the length-weight relationship parameters. Mean population size of females were 0.72 cm taller than the males, however difference was not significant (t-test, P > 0.05). The overall relationship equations between total length (TL) and body weight (BW) were established for males as Log TW = 2.71 Log TL - 1.85 (R2 = 0.95) and for females as Log TW = 2.88 Log TL-2.10 (R2 = 0.95). The estimated relative growth co-efficient (b) values were 2.71 for males and 2.88 for females. It is revealed that growth pattern of the species showed negative allometry. In both males and females, relationship between TL and SL gave highest regression coefficient (0.99). While relationship between TL and EL gave lowest regression coefficient in both males and females (0.87 and 0.81 respectively). The findings from this study contributed first information on morphometric relations of the fish from Malaysian coastal waters and could be useful for sustainable management options of P. canius in Malaysia.
A survey of Sybil attack countermeasures in IoT-based wireless sensor networks

Arshad A, Mohd Hanapi Z, Subramaniam S, Latip R

PeerJ Comput Sci, 2021;7:e673.
PMID: 34712787 DOI: 10.7717/peerj-cs.673

Wireless sensor networks (WSN) have been among the most prevalent wireless innovations over the years exciting new Internet of Things (IoT) applications. IoT based WSN integrated with Internet Protocol IP allows any physical objects with sensors to be connected ubiquitously and send real-time data to the server connected to the Internet gate. Security in WSN remains an ongoing research trend that falls under the IoT paradigm. A WSN node deployed in a hostile environment is likely to open security attacks such as Sybil attack due to its distributed architecture and network contention implemented in the routing protocol. In a Sybil attack, an adversary illegally advertises several false identities or a single identity that may occur at several locations called Sybil nodes. Therefore, in this paper, we give a survey of the most up-to-date assured methods to defend from the Sybil attack. The Sybil attack countermeasures includes encryption, trust, received signal indicator (RSSI), encryption and artificial intelligence. Specifically, we survey different methods, along with their advantages and disadvantages, to mitigate the Sybil attack. We discussed the lesson learned and the future avenues of study and open issues in WSN security analysis.
Comparative analysis of IVF-ICSI outcomes between advanced and early stage of endometriosis stimulated with HMG

Arshad AH, Azrai Abu M, Ahmad MF, Abdul Karim AK

Med J Malaysia, 2022 Jan;77(1):1-5.
PMID: 35086987

INTRODUCTION: Endometriosis is a challenging disease to treat, and patients may eventually need in vitro fertilisation with Intracytoplasmic sperm injection (IVF/ICSI) to conceive after other modalities failed. There are inconsistent outcomes of IVF performance in patients with endometriosis especially with highly purified human menotropin gonadotrophin (hMG). This study was commenced to determine whether the use of hMG affects the IVF outcome in different stage of endometriosis.
MATERIALS AND METHODS: This is an observational study. Eighty-seven women who had endometriosis confirmed surgically and underwent IVF/ICSI treatment, stimulated with hMG alone were included. Based on the revised American Society for Reproductive Medicine (rASRM), the participants were classified as early endometriosis (I/II) (n=39) or advanced endometriosis (III/IV) (n=35). The main outcome measures used were clinical pregnancy rate.
RESULTS: Women with advanced endometriosis had a lower oocyte yield, less good quality day-3 embryos and lower clinical pregnancy rate compared with the mild endometriosis. However, higher fertilisation rate were recorded in advanced stage endometriosis compared to milder disease.
CONCLUSIONS: The rASRM classification of endometriosis is valuable in predicting IVF outcome as advanced endometriosis performs poorly compared to a milder disease. Highly purified hMG could be an alternative as an ovarian stimulation in endometriosis.
Sex ratio, spawning season and the size at maturity of Parapenaeopsis sculptilis (Heller 1862) in the coastal waters of Perak, Peninsular Malaysia

Arshad A, Amani AA, Amin SMN, Yusoff FM

J Environ Biol, 2016 07;37(4 Spec No):709-13.
PMID: 28779730

Parapenaeopsis sculptilis (Heller, 1862) locally referred to as ?udang kulit keras? in Malaysia has profound biological, ecological, aquacultural and conservational significance. The reproductive biology of this important penaeid from the coastal waters of Perak, Peninsular Malaysia, was studied during the period between February 2012 to January 2013. Females outnumbered males with a sex ratio of M: F= 1:3 (P < 0.05). Four maturity stages of female gonads viz., immature, maturing, mature and spent stages were distinguished. The first stage of sexual maturity was attained at a length of 9.3 cm, and female P. sculptilis showed a peak gonadosomatic index during the month of April, August and October, indicating that P. sculptilis potentially breeds throughout the year. The findings of this study would greatly contribute towards the understanding of gonadal maturation, spawning season and breeding biology, which could be important for the effective population management of this prawn species.
Comparison of condition index in the green-lipped mussel Perna viridis between Sebatu and Pasir Panjang, Peninsular Malaysia

Al-Barwani SM, Arshad A, Amin SMN, Rahman A

J Environ Biol, 2016 07;37(4 Spec No):705-8.
PMID: 28779729

The condition index (which relates to the tissue dry weight to shell volume) of the green-lipped mussel Perna viridis was investigated at Sebatu in Melaka and Pasir Panjang in Negri Sembilan from September 2003 to February 2004. Monthly samples of P. viridis were collected from culturing rafts at both sites. Variations in the average monthly condition index (CI) of P. viridis ranged from 21.06 to 26.72 g cm(-3) and 15.18 to 19.41 g cm(-3) in Sebatu and Pasir Panjang, respectively. Salinity values were lowest in November between 27.93 to 28.10 ppt in Sebatu and Pasir Panjang, respectively. Salinity then started increasing gradually until it reached 33.26 ppt in Sebatu and 31.23 ppt in Pasir Panjang in the month of February. Chlorophylla in Pasir Panjang showed higher fluctuation, ranging from 2.93 to 14.39 mg/L, while in Sebatu the fluctuations were lower and ranged between 7.70 and 9.37 mg l(-1). Rapid decline in CI values were recorded during January in Sebatu and February in Pasir Panjang. However, this rapid declining state of CI in P. viridis was an indication of its spawning period, when their gametes were released in the water column. These findings would help in the development of captive breeding techniques and mass seed production in aquaculture.
Composition and diversity of larval fish in the mangrove estuarine area of Marudu Bay, Sabah, Malaysia

Rezagholinejad S, Arshad A, Amin SMN, Ara R

J Environ Biol, 2016 07;37(4 Spec No):697-703.
PMID: 28779728

The composition of fish larvae and their diversity in different habitats are very important for fisheries management. Larval fishes were investigated in a mangrove estuary of Marudu Bay, Sabah, Malaysia from October 2012 to September 2013 at five different sites. Monthly samples of fish larvae were collected at five sampling sites by a plankton net with a mouth opening of 40.5 cm in diameter. In total, 3879 larval fish were caught in the investigated area. The mean density of ichthyoplankton at this area was 118 larvae/100 m(3). The fish larval assemblage comprised of 20 families whereas 13 families occurred at St1, 16 at St2, 16 at St3, 12 at St4 and 16 at St5. The top major families were Sillaginidae, Engraulidae, Mugilidae and Sparidae with Sillaginidae consisted 44% of total larval composition. St3 with 143 larvae/100 m(3) had the highest density amongst the stations which was due to higher abundance of Sillaginidae. Shannon-Wiener diversity index represented significant variation during monsoon and inter-monsoon seasons, peaking in the months December-January and May-June. However, Shannon-Wiener index, evenness and family richness showed significant differences among stations and months (p < 0.05).
Food and feeding habits of Omobranchus sp. (Blenniidae: Omobranchini) larvae in the Seagrass-Mangrove ecosystem of Johor Strait, Malaysia

Ara R, Arshad A, Amin SMN, Ghaffar MA

J Environ Biol, 2016 07;37(4 Spec No):735-43.
PMID: 28779733

The stomach contents of Omobranchus sp. (family Blenniidae) larvae were investigated in a seagrass-mangrove based ecosystem in Johor Strait, Malaysia from October 2007 to September 2008. Specimens of larval fish were collected through subsurface towing of a Bongo net from five different stations. The stomach sacs of 267 Omobranchus sp. larvae were separated and observed, which comprised of 24 significant food stuffs belonging to 6 main groups viz. phytoplankton (62.45%), zooplankton (18.24%), algae (5.56%), plant-like particles (5.75%), debris (4.22%) and unidentified particles (2.03%). In situ water parameters were also measured throughout the sampling cruises. There was a strong and significant positive correlation between stomach phytoplankton and salinity (r = 0.658, p < 0.05).? Canonical correlation analysis indicated a weak relationship (29.8%) between stomach contents and physico-chemical parameters. Only salinity appeared to be the controlling factor for the stomach contents of Omobranchus sp. larvae in the investigated area. Based on the stomach content analysis, it could be concluded that Omobranchus sp. were mainly herbivorous during the larval stages. ?
Sex ratio, gonad development and fecundity of Miyakella nepa (Crustacea, Stomatopoda) of Pantai Remis coastal waters of Malaysia

Zamri Z, Arshad A, Amin SMN, Rahman MA, Al Khayat JA

J Environ Biol, 2016 07;37(4 Spec No):677-83.
PMID: 28779726

The sex ratio, gonad development and fecundity of Miyakella nepa (Latreille, 1828), in the coastal waters of Pantai Remis, Perak, were investigated from February 2012 to January 2013. Sex identification was done by identifying stomatopod's genitalia organs, and the ovaries were dissected out and preserved for further analysis. Female stomatopods with mature or near spawning stages were used for fecundity estimation. A total of 951 specimens of M. nepa, with 565 females and 386 males were examined. Results showed a sex ratio of 1:1.46 (male:females). Maximum GSI was estimated to be 5.80, while lowest was 2.95. Sexual maturity for female M. nepa was observed at 100 mm total length. The mean fecundity of M. nepa was 425,657 (? 1,8701) eggs which was observed to increase with increased body length.
Inner shell as variation key of local hard clam Meretrix spp

Hamli H, Idris MH, Abu Hena MK, Rajaee AH, Arshad A

J Environ Biol, 2016 07;37(4 Spec No):641-6.
PMID: 28779722

The morphology and 12 shell morphometric features proportionate to shell length were analysed between local hard clam; Meretrix lyrata, M. meretrix and M. lusoria from Sarawak, Malaysia. Meretrix spp. was observed to comprise a unique feature of a pallial sinus scar for each species. Analysis of variance revealed significant differences among Meretrix spp. using proportion ratios of SL for SW; LL; AL; LCT; AW; PW and PS (p<0.05). Cluster analysis among morphometric features of M. lyrata, M. meretrix and M. lusoria were discriminated at 98.5% similarities and supported by the principal component analysis. The present study suggests that pallial sinus scar shape, together with interior and exterior morphometric features, were suitable as identification keys for Meretrix spp. Hence, the present study emphasizes on the application of interior, rather than exterior morphology and morphometric features in hard clam identification before further investigation can be performed through genetic identification means.

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