Displaying publications 341 - 360 of 621 in total

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  1. Chinnambedu RS, Marimuthu RR, Sunil SS, Amrose P, Ramachandran V, Pachamuthu B
    J Infect Public Health, 2020 Jan;13(1):75-79.
    PMID: 31402312 DOI: 10.1016/j.jiph.2019.06.015
    PURPOSE: Emergence of multidrug-resistant and methicillin-resistant Staphylococcus aureus (MRSA) infections in HIV patients limit the treatment options and challenge the clinical management of infections. The periodic monitoring of S. aureus infections and its drug resistance profile in HIV patients are of paramount importance in clinical management.

    MATERIALS AND METHODS: A total of 7204 clinical specimens from HIV patients from 2012 to 2017 were processed for the isolation of S. aureus strains using conventional culture techniques and cultures were identified using standard biochemical test. Antibiotic susceptibility of S. aureus strains was tested by Kirby-Bauer disk diffusion method.

    RESULTS: A total of 380 (5.3%) S. aureus strains were isolated from HIV patients in the study period. High percentage of S. aureus strains were isolates from urine (69.5%) specimen and 58.4% of S. aureus infections were noted among hospitalized patients. Antibiotic susceptibility profile reveals S. aureus was highly resistant to penicillin (95.2%) followed by cephalexin (84.6%). Methicillin resistance was highly observed in the year 2017 (86%) and the rate of MRSA steadily increasing from 51.8% in 2012 to 86% in 2017. Significant increase of S. aureus infections (35%; p<0.001) and MRSA (76%; p=0.0007) were observed in the year 2016.

    CONCLUSIONS: This study reports the increasing trends of S. aureus infections and MRSA among HIV patients from Southern India. Multidrug-resistance profile of S. aureus could complicate the selection of proper antibiotic regimens and time cure of HIV patients.

    Matched MeSH terms: Staphylococcus aureus/drug effects*; Methicillin-Resistant Staphylococcus aureus/drug effects
  2. Makaremi M, Yousefi H, Cavallaro G, Lazzara G, Goh CBS, Lee SM, et al.
    Polymers (Basel), 2019 Sep 29;11(10).
    PMID: 31569482 DOI: 10.3390/polym11101594
    Extensive usage of long-lasting petroleum based plastics for short-lived application such as packaging has raised concerns regarding their role in environmental pollution. In this research, we have developed active, healable, and safely dissolvable alginate-pectin based biocomposites that have potential applications in food packaging. The morphological study revealed the rough surface of these biocomposite films. Tensile properties indicated that the fabricated samples have mechanical properties in the range of commercially available packaging films while possessing excellent healing efficiency. Biocomposite films exhibited higher hydrophobicity properties compared to neat alginate films. Thermal analysis indicated that crosslinked biocomposite samples possess higher thermal stability in temperatures below 120 °C, while antibacterial analysis against E. coli and S. aureus revealed the antibacterial properties of the prepared samples against different bacteria. The fabricated biodegradable multi-functional biocomposite films possess various imperative properties, making them ideal for utilization as packaging material.
    Matched MeSH terms: Staphylococcus aureus
  3. Ahmed D, Anwar A, Khan AK, Ahmed A, Shah MR, Khan NA
    AMB Express, 2017 Nov 21;7(1):210.
    PMID: 29164404 DOI: 10.1186/s13568-017-0515-x
    Biofilm formation by pathogenic bacteria is one of the major threats in hospital related infections, hence inhibiting and eradicating biofilms has become a primary target for developing new anti-infection approaches. The present study was aimed to develop novel antibiofilm agents against two Gram-positive bacteria; Staphylococcus aureus (ATCC 43300) and Streptococcus mutans (ATCC 25175) using gold nanomaterials conjugated with 3-(diphenylphosphino)propionic acid (Au-LPa). Gold nanomaterials with different sizes as 2-3 nm small and 9-90 nm (50 nm average size) large were stabilized by LPa via different chemical synthetic strategies. The nanomaterials were fully characterized using atomic force microscope (AFM), transmission electron microscope, ultraviolet-visible absorption spectroscopy, and Fourier transformation infrared spectroscopy. Antibiofilm activity of Au-LPa nanomaterials was tested using LPa alone, Au-LPa and unprotected gold nanomaterials against the both biofilm-producing bacteria. The results showed that LPa alone did not inhibit biofilm formation to a significant extent below 0.025 mM, while conjugation with gold nanomaterials displayed manifold enhanced antibiofilm potential against both strains. Moreover, it was also observed that the antibiofilm potency of the Au-LPa nanomaterials varies with size variations of nanomaterials. AFM analysis of biofilms further complemented the assay results and provided morphological aspects of the antibiofilm action of Au-LPa nanomaterials.
    Matched MeSH terms: Staphylococcus aureus
  4. Niek WK, Teh CSJ, Idris N, Thong KL, Ponnampalavanar S
    Jpn J Infect Dis, 2019 Jul 24;72(4):228-236.
    PMID: 30814457 DOI: 10.7883/yoken.JJID.2018.289
    Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common nosocomial pathogens, causing mild to severe infections. This study aimed to determine the genotypic and phenotypic characteristics of clinical MRSA isolates collected from a teaching hospital from 2014 - 2015. These isolates were genotyped by multilocus sequence typing, staphylococcal cassette chromosomal mec (SCCmec) typing, virulence genes detection, and pulsed-field gel electrophoresis; they were phenotyped based on their antibiotics susceptibility profiles. The most prevalent sequence type was ST22. ST3547 was identified from a blood isolate from 2015. Three SCCmec types (III in 26.26%, IV in 70.71%, and V in 3.03% isolates) were detected. Agr type I, II, and III were also detected among the isolates. The most prevalent virulence genes found were hemolysin (100%) and intracellular adhesion (91.9%). At least one staphylococcal enterotoxin was detected in 83 (83.8%) isolates. All the isolates were susceptible to vancomycin (minimum inhibitory concentration ≤ 2 μg/mL). Statistical analysis revealed a significant increase in hypertension (p = 0.035), dyslipidemia and obesity (p = 0.046), and previous exposure to any quinolone (p = 0.010) cases over the two-year period. The emergence and circulation of community-associated MRSA variants were observed in our hospital.
    Matched MeSH terms: Methicillin-Resistant Staphylococcus aureus/classification; Methicillin-Resistant Staphylococcus aureus/genetics*; Methicillin-Resistant Staphylococcus aureus/isolation & purification
  5. Normah Haron, Hanapi Mat Jusoh
    MyJurnal
    Gynura procumbens, commonly known as ‘sambung nyawa’ in Malaysia has been used
    traditionally as remedies for anti-inflammatory, anti-hyperlipidimic and anti-hyperglycemic. The
    purpose of the present study was to qualitatively evaluate the antimicrobial effects of Gynura procumbens extracts. effects of the sample were determined by disc diffusion method against two bacteria and two fungi namely E. coli, S. aureus, C. albicans and S. cerevisiae. The results showed that the acidic extract of G. procumbens has positive reactions towards E. coli, S. aureus and C. albicans with the presence of zone of inhibition at the concentration of 150 mg/mL. Following the positive reaction, the minimum inhibitory concentration (MIC) of the acidic extract was then evaluated by broth dilution method. The MIC of E. coli and S. aureus were determined at concentration 37.5 mg/mL and 75 mg/mL for C. albicans. It indicated that acidic extracts at lower concentration could inhibit the bacteria, but high concentration of extracts was required in the inhibition of the fungi. It can be concluded that, the present study proves that there is potential of antimicrobial effects in Gynura procumbens leaves extracts.
    Matched MeSH terms: Staphylococcus aureus
  6. Tan TL, Lim SH, Ruslan Mustapa M, Ganeswary R
    Med J Malaysia, 2020 11;75(6):742-744.
    PMID: 33219189
    Methicillin-resistant Staphylococcus aureus (MRSA) purulent pericarditis, characterised by frank pus collection or microscopic pyogenic effusion in the pericardium represents the most serious form of pericardial infection. The route of MRSA acquisition in pericardial abscess commonly occurs via the blood stream infection and it is more commonly observed among immunocompromised individuals. To date, diabetic foot ulcer infection rarely disseminates and becomes a nidus for pericardial infection. Herein, we report an unusual case of MRSA pericardial abscess in a 44-year-old man who presented at Hospital Seri Manjung, Malaysia with cardiac tamponade. Past medical history indicated that he was recently treated for infected diabetic foot ulcer with MRSA bacteraemia one week earlier. Despite adequate pericardial drainage and extended parenteral vancomycin therapy, this case ended in fatality on day 42 of admission due to nosocomial infection. It is hoped that this report serves to increase the vigilance among clinicians that diabetic foot ulcer infections have the potential to progress to pericardial abscess in the presence of MRSA bacteraemia, although they may appear seemingly innocuous at presentation. Systemic vancomycin must be instituted promptly when MRSA bacteraemia is confirmed in order to circumvent the propagation of MRSA.
    Matched MeSH terms: Methicillin-Resistant Staphylococcus aureus
  7. Harnentis H, Marlida Y, Nur YS, Wizna W, Santi MA, Septiani N, et al.
    Vet World, 2020 Sep;13(9):1922-1927.
    PMID: 33132606 DOI: 10.14202/vetworld.2020.1922-1927
    Background and Aim: Probiotics play an important role in maintaining a healthy gut and consequently promote good health. This study aimed to find novel probiotic lactic acid bacteria (LAB) from indigenous fermented foods of West Sumatera, Indonesia.

    Materials and Methods: This study utilized 10 LAB previously isolated from fermented buffalo milk (dadih), fermented fish (budu), and fermented cassava (tape) which have the ability to produce gamma-aminobutyric acid. The study commenced with the screening of LAB for certain properties, such as resistance to acid and bile salts, adhesion to mucosal surface, and antagonism against enteric pathogens (Escherichia coli, Salmonella Enteritidis, and Staphylococcus aureus). The promising isolates were identified through biochemical and gram staining methods.

    Results: All isolates in this study were potential novel probiotics. They survived at a pH level of 2.5 for 3 h (55.27-98.18%) and 6 h (50.98-84.91%). Survival in bile at a concentration of 0.3% was 39.90-58.61% and the survival rate was 28.38-52.11% at a concentration of 0.5%. The inhibitory diameter ranged from 8.75 to 11.54 mm for E. coli, 7.02 to 13.42 mm for S. aureus, and 12.49 to 19.00 mm for S. Enteritidis. All the isolates (84.5-92%) exhibited the ability to adhere to mucosal surfaces. This study revealed that all the isolates were potential probiotics but N16 proved to be superior because it was viable at a pH level of 2 (84.91%) and it had a good survival rate in bile salts assay (55.07%). This isolate was identified as Lactobacillus spp., Gram-positive bacilli bacteria, and tested negative in both the catalase and oxidase tests.

    Conclusion: All the isolates in this study may be used as probiotics, with isolate N16 (Lactobacillus spp.) as the most promising novel probiotic for poultry applications based on its ability to inhibit pathogenic bacteria.

    Matched MeSH terms: Staphylococcus aureus
  8. Hanini ‘Aina, Nik Nur Shamiha Nik Dzulkefli, Mohamed Rasny, Samer Al-Dhalli, Mohd Nizam, Eddy Yusuf, et al.
    MyJurnal
    The composition of ophthalmic preparation is administered topically to the eye in the form of a solution, suspension, ointment, gel or foam for the purpose of treating eye disease. Virgin Coconut Oil (VCO) has been one of the desired ingredients of choice, as its benefits as functional food oil is known among the public. The uniqueness of coconut oil is its fats and oils that contain the highest percentage of medium-chain fatty acids (MCFA), which have antimicrobial properties, such as lauric acid and capric acid. This study aimed to evaluate the antimicrobial effects of eye drop containing a different VCO concentration using the Kirby-Bauer test. The formulation of eye drop had 1.5%, 2% and 3% amount of virgin coconut oil, which was later added with all basic materials needed for eye drop. The samples were evaluated for its zone of inhibition (ZOI). The antimicrobial effects of eye drop formulation that contains 3% of VCO (F3) against Streptococcus pneumonia were similar with all control products (Eye Glo, Pred Forte, Cationorm), and antimicrobial effects of F3 against Staphylococcus aureus are better than Cationorm. It is noticeable that the higher the VCO content in the formulation, the better the antimicrobial effects of the eye drop. In conclusion, VCO possesses moisture, anti-inflammation, better anti-microbial properties, and it could be further formulated as a stable eye drop emulsion.
    Matched MeSH terms: Staphylococcus aureus
  9. Harika K, Shenoy VP, Narasimhaswamy N, Chawla K
    J Glob Infect Dis, 2020 08 29;12(3):129-134.
    PMID: 33343163 DOI: 10.4103/jgid.jgid_150_19
    Background: Microorganisms are known to be involved in the formation of biofilm. These biofilms are often seen in chronic wound infections, surgical site infections, implants etc., These are capable of causing recalcitrant infections and most of them are also known to possess high antibiotic resistance.

    Objectives: This study was conducted to detect the biofilm formation in bacterial isolates from chronic wound infections.

    Materials and Methods: In the present study, ninety two isolates from chronic wound infections were identified by MALDI-TOF-MS (bioMerieux) and VITEK-2-MS (bioMerieux). These isolates were further screened for biofilm formation by three methods i. e., Tissue Culture Plate method (TCP), Tube Method (TM) and Congo Red Agar (CRA) method. Impact of biofilm production was correlated with the antibiotic resistant pattern.

    Statistical Analysis: Statistical analysis was done for all three methods considering TCP as Gold Standard and parameters like senitivity and specificity of TM i.e. 47.2 and 100% respectively.

    Results: Out of 92 isolates, biofilm formation was seen in 72 isolates (78.2%) by TCP method. 64 isolates were strong biofilm producers, 8 isolates were moderate biofilm producers and 20 isolates were nonbiofilm producing. High prevalence of biofilm formation was seen in nonhealing ulcers infected with Staphylococcus aureus followed by Klebsiella pneumoniae.

    Conclusion: Among three screening methods used for detection of biofilm production, TCP method is considered to be a standard and most reliable for screening of biofilm formation in comparison to TM and CRA.

    Matched MeSH terms: Staphylococcus aureus
  10. Mansor, R., Diauudin, N.S., Syed-Hussain, S.S., Khalid, S.F.
    Jurnal Veterinar Malaysia, 2019;31(1):12-16.
    MyJurnal
    Antimicrobial resistance (AMR) has become a major problem worldwide with significance public health impact in both animal and human populations. Staphylococcus aureus (S. aureus) is a common pathogen in domestic livestock and Escherichia coli (E. coli) is a normal flora in the gut and these organisms are considered as effective indicators for AMR surveillance. Increased in antimicrobial resistance of these bacteria in veterinary medicine leads to difficulties in choosing effective antibiotics to treat diseases they caused. This study aimed to evaluate the susceptibility of S. aureus and E. coli isolated from dairy goats in selected farms located in Selangor, Malaysia against different antibiotics. Milk and faecal samples were collected from 36 dairy goats and samples were prepared for the isolation of S. aureus and E. coli isolates respectively. The isolates were subjected to antibiotic susceptibility test. All 11 (100%) E. coli isolates showed resistance to amoxicillin and penicillin while 3 (27%) of the 11 S. aureus isolates, showed resistance towards the same class of antibiotic. Both S. aureus and E. coli isolates showed high susceptibility to four antibiotics, namely trimethoprim-sulfamethazole, neomycin, tetracycline and enrofloxacin. This study provided information regarding the antibiotic resistance of S. aureus and E. coli in relation to the antimicrobial usage practice in selected dairy goat farms located in Selangor, Malaysia.
    Matched MeSH terms: Staphylococcus aureus
  11. Jamal F, Minhaj AA, Kasim MS, Zainal Z, George R
    Family Practitioner, 1985;8(2):53-56.
    Bacterial skin infection was studied in 239 children between the ages of 1 and 12 years in an urban squatter area in Kuala Lumpur over a period of three months (January - March 1983). 62 children presented with dermatological problems, 33 of which were clinically diagnosed as pyoderma. Microbiological examination of swabs from the lesions yielded mixed growth from 54.5% (18/33) specimens. Beta-haemolytic streptococci were isolated from 63.6% (21/33) and Staphylococcus aureus from 54.5% (18/33) specimens. All streptococcal isolates were grouped with a commercial kit, the Streptex (Wellcome). 81% (17/21) belonged to Lancefield's group A, 90% of which were not M or T typable by the standard international set of antisera. Urine analysis, pharyngeal swab culture and determination of anti-streptococcal antibody titre were also performed for children with positive skin cultures for group A streptococcus. 6% (2/33) of children with impetigo developed acute glomerulonephritis, requiring hospitalization.
    Study site: Growth and nutrition clinic, squatter area, Kuala Lumpur, Malaysia
    Matched MeSH terms: Staphylococcus aureus
  12. Kamaruzzaman NF, Pina MF, Chivu A, Good L
    Polymers (Basel), 2018 May 12;10(5).
    PMID: 30966555 DOI: 10.3390/polym10050521
    The treatment of skin and soft tissue infections caused by methicillin-resistant Staphylococcus aureus (MRSA) remains a challenge, partly due to localization of the bacteria inside the host's cells, where antimicrobial penetration and efficacy is limited. We formulated the cationic polymer polyhexamethylene biguanide (PHMB) with the topical antibiotic nadifloxacin and tested the activities against intracellular MRSA in infected keratinocytes. The PHMB/nadifloxacin nanoparticles displayed a size of 291.3 ± 89.6 nm, polydispersity index of 0.35 ± 0.04, zeta potential of +20.2 ± 4.8 mV, and drug encapsulation efficiency of 58.25 ± 3.4%. The nanoparticles killed intracellular MRSA, and relative to free polymer or drugs used separately or together, the nanoparticles displayed reduced toxicity and improved host cell recovery. Together, these findings show that PHMB/nadifloxacin nanoparticles are effective against intracellular bacteria and could be further developed for the treatment of skin and soft tissue infections.
    Matched MeSH terms: Methicillin-Resistant Staphylococcus aureus
  13. Fahad S, Habib AA, Awais MB, Umer M, Rashid HU
    Malays Orthop J, 2019 Mar;13(1):36-41.
    PMID: 31001382 DOI: 10.5704/MOJ.1903.006
    Introduction: Tibia is the most common long bone fractured due its vulnerable subcutaneous location and most often associated with acquired complications of delayed union or non-union due to infection. Amongst the various treatment options to treat them, the Ilizarov external fixator application is considered superior due to its multiple advantages. The objective of this study was to analyse the role of Ilizarov fixation in infected tibial non-union, as well as to assess bony union and associated functional outcomes. Materials and Methods: A retrospective review was conducted for the duration between 1st January 2005 to 31st December 2016. Total of fifty-one patients with tibial non-union associated with infection who treated with the Ilizarov fixator were included in the study. Patient records were reviewed for union of bone, bone and functional outcomes and complications. Results: The most common organism for infection was identified to be Staphylococcus Aureus. At the time of final follow-up all patients had achieved union except two, one of whom had to undergo amputation due to non-union and sepsis. Majority of the patients had an excellent score as per ASAMI grading system for bone and function results. The most common complication noted was pin track infections. Conclusion: In our experience, Ilizarov external fixator is better suited for infected non-union of tibia because it can provide a stable mechanical environment, bone transport, correct deformities, and enable weight bearing and hence we recommend its use for the same.
    Matched MeSH terms: Staphylococcus aureus
  14. Adnan SN, Ibrahim N, Yaacob WA
    Germs, 2017 Dec;7(4):186-192.
    PMID: 29264356 DOI: 10.18683/germs.2017.1125
    Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide public health threat, displaying multiple antibiotic resistance that causes morbidity and mortality. Management of multidrug-resistant (MDR) MRSA infections is extremely difficult due to their inherent resistance to currently used antibiotics. New antibiotics are needed to combat the emergence of antimicrobial resistance.

    Methods: The in vitro effect of tannins was studied against MRSA reference strain (ATCC 43300) and MRSA clinical strains utilizing antimicrobial assays in conjunction with both scanning and transmission electron microscopy. To reveal the influence of tannins in MRSA protein synthesis disruption, we utilized next-generation sequencing (NGS) to provide further insight into the novel protein synthesis transcriptional response of MRSA exposed to these compounds.

    Results: Tannins possessed both bacteriostatic and bactericidal activity with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 0.78 and 1.56 mg/mL, respectively, against all tested MRSA. Scanning and transmission electron microscopy of MRSA treated with tannins showed decrease in cellular volume, indicating disruption of protein synthesis.

    Conclusion: Analysis of a genome-wide transcriptional profile of the reference strain ATCC 43300 MRSA in response to tannins has led to the finding that tannins induced significant modulation in essential ribosome pathways, which caused a reduction in the translation processes that lead to inhibition of protein synthesis and obviation of bacterial growth. These findings highlight the potential of tannins as new promising anti-MRSA agents in clinical application such as body wash and topical cream or ointments.

    Matched MeSH terms: Methicillin-Resistant Staphylococcus aureus
  15. Khan TM, Kok YL, Bukhsh A, Lee LH, Chan KG, Goh BH
    Germs, 2018 Sep;8(3):113-125.
    PMID: 30250830 DOI: 10.18683/germs.2018.1138
    Background: Burn victims admitted in burn intensive care units (ICU) are at a high risk of nosocomial infections generated by methicillin resistant Staphylococcus aureus (MRSA). This systematic review aims to estimate the incidence of MRSA among burn patients admitted to the ICU setting, with an emphasis on the incidence rate and antibiotic resistance profile of the MRSA strains.

    Methods: A systematic literature search was performed in five electronic databases limited to publication dates from 1st January 2000 until 31st August 2017. After screening n=481 articles, n=21 were found to meet the inclusion criteria of this systematic review.

    Results: Results from the meta-analysis revealed that the risk for MRSA isolates in the burn ICU was 55.0% higher (OR 0.55, 95%CI 0.32-0.94). Therefore, timely testing, appropriate hygiene practice and suggested wound care must be practiced while handling such patients.

    Conclusion: Further studies are needed to identify the risk factors of MRSA infections among burn patients and to develop new antimicrobial agents for MRSA infections.

    Matched MeSH terms: Methicillin-Resistant Staphylococcus aureus
  16. Sapri HF, Ismail MAH, Sani NAM, Noordin A, Tan TL, Hussin S, et al.
    Germs, 2020 Jun;10(2):104-111.
    PMID: 32656107 DOI: 10.18683/germs.2020.1191
    Introduction: We report the results of a molecular surveillance study carried out on methicillin-susceptible Staphylococcus aureus (MSSA) isolated in a one-year duration from Hospital Canselor Tuanku Muhriz (HCTM), a tertiary hospital located in Kuala Lumpur, Malaysia.

    Methods: The first strain isolated from each MSSA infection in HCTM during the year 2009 was included into the study. Antimicrobial susceptibility testing and agr group typing were carried out for all strains; virulence gene (cna, seh, TSST-1 and PVL) typing results of the strains were obtained from a previous study. Pulsed-field gel electrophoresis (PFGE) was done on selected strains from the orthopedic ward. Relationship(s) between different typing methods used in the study was investigated, where a p value of <0.05 indicated significant association between typing methods.

    Results: A total of 880 MSSA strains were included into the study. The strains were generally susceptible to most antibiotics, with most of them carrying cna and agr-I (51.6%, n=454; 39.8%, n=350, respectively). A total of 17 PFGE pulsotypes were identified using an 80% similarity cut-off value, where the main pulsotype (pulsotype E) consisted of 24 isolates (23.5%). agr-III strains were found to be usually positive for both cna and seh (p<0.05). In addition, some PFGE pulsotypes were also characteristic of certain virulence genes or agr groups.

    Conclusions: We did not identify a dominant MSSA clone circulating in HCTM in 2009. Nevertheless, results from this molecular surveillance will provide good baseline data for the hospital's second S. aureus surveillance planned for the year 2020.

    Matched MeSH terms: Staphylococcus aureus
  17. Amirah Nadiah Ali, Mohd Syahmi Salleh, Ahmad Fahmi Harun, Muhamad Ashraf Rostam
    MyJurnal
    Increasing risk of antibiotic resistance of pathogenic bacteria has led to the exploration of alternative antibiotics derived from leaves of medicinal plants such as the fig (Ficus carica L.). The aim of this study was to determine the antimicrobial activity of the methanolic extract of fig leaves grown under Malaysian tropical environment against pathogenic bacteria linked to antibiotic resistance namely the Staphylococcus aureus and Escherechia coli. The methanolic extraction was performed by using soxhlet apparatus. The disc diffusion method was used to measure inhibition zone diameter on the Mueller-Hinton agar plate. Staphylococcus aureus displayed the highest diameter of inhibition zone against the extract at concentration of 900 mg/ml whilst Escherechia coli displayed the highest diameter of inhibition zone against both the 100% crude extract and 700 mg/ml, respectively. This study therefore highlighted the potential of developing alternative antibiotics derived from the methalonic extract of locally grown fig plant.
    Matched MeSH terms: Staphylococcus aureus
  18. Aziman N, Kian LK, Jawaid M, Sanny M, Alamery S
    Polymers (Basel), 2021 Jan 27;13(3).
    PMID: 33513665 DOI: 10.3390/polym13030391
    The development of antimicrobial film for food packaging application had become the focus for researchers and scientists. This research aims to study the characteristics and antimicrobial activity of novel biofilms made of poly (butylene succinate) (PBS) and tapioca starch (TPS) added with 1.5% or 3% of Biomaster-silver (BM) particle. In morphological examination, the incorporation of 3% BM particle was considerably good in forming well-structured PBS film. Meanwhile, the functional groups analysis revealed the 3% BM particle was effectively interacted with PBS molecular chains. The flame retard behavior of BM metal particle also helped in enhancing the thermal stability for pure PBS and PBS/TPS films. The nucleating effect of BM particles had improved the films crystallinity. Small pore size features with high barrier property for gas permeability was obtained for BM filled PBS/TPS films. From antimicrobial analysis, the BM particles possessed antimicrobial activity against three bacteria Staphylococcus aureus, Escherichia coli, and Salmonella Typhimurium in which PBS/TPS 3% BM film exhibited strong antimicrobial activity against all tested bacteria, however, PBS/TPS 1.5% BM film exhibited strong antimicrobial activity against E. coli only. Hence, the incorporation of BM into PBS/TPS film could be a sustainable way for developing packaging films to preserve food products.
    Matched MeSH terms: Staphylococcus aureus
  19. Ghanbari R, Ebrahimpour A
    Food Sci Biotechnol, 2018 Apr;27(2):591-598.
    PMID: 30263784 DOI: 10.1007/s10068-017-0267-z
    Actinopyga lecanora, as a rich protein source was hydrolysed to generate antibacterial bioactive peptides using different proteolytic enzymes. Bromelain hydrolysate, after 1 h hydrolysis, exhibited the highestantibacterial activities against Pseudomonas aeruginosa, Pseudomonas sp., Escherichia coli and Staphylococcus aureus. Two dimensional fractionation strategies, using a semi-preparative RP-HPLC and an isoelectric-focusing electrophoresis, were applied for peptide profiling. Furthermore, UPLC-QTOF-MS was used for peptides identification; 12 peptide sequences were successfully identified. The antibacterial activity of purified peptides from A. lecanora on P. aeruginosa, Pseudomonas sp., E. coli and S. aureus was investigated. These identified peptides exhibited growth inhibition against P. aeruginosa, Pseudomonas sp., E. coli and S. aureus with values ranging from 18.80 to 75.30%. These results revealed that the A. lecanora would be used as an economical protein source for the production of high value antibacterial bioactive peptides.
    Matched MeSH terms: Staphylococcus aureus
  20. Zainal M, Mohamad Zain N, Mohd Amin I, Ahmad VN
    Saudi Dent J, 2021 Feb;33(2):105-111.
    PMID: 33551624 DOI: 10.1016/j.sdentj.2020.01.008
    The objective of this study is to determine the therapeutic efficacy of allicin against Candida albicans (C. albicans) and Staphylococcus aureus (S. aureus), the common etiological agents for denture stomatitis (DS). The minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentration (MFCs) of allicin were determined by the broth microdilution method followed by checkerboard microdilution method for a synergistic interaction between allicin + nystatin and allicin + CHX. The potential of allicin to eradicate C. albicans and S. aureus biofilms was assessed by treating biofilm formed on self- polymerized acrylic resin with allicin at a sub-MIC concentration for 5 min. The commercial denture cleanser (brand X) was used as a positive control. A Kruskal-Wallis test followed by the post-hoc Mann-Whitney U test was applied (SPSS 20.0), and the level of significance was set at P 
    Matched MeSH terms: Staphylococcus aureus
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