MyMedR

Displaying publications 201 - 220 of 333 in total

Abstract:

Sort:

Fulltext Multidrug-resistant Streptococcus pneumoniae causing invasive pneumococcal disease isolated from a paediatric patient

Arushothy R, Ramasamy H, Hashim R, Raj A S S, Amran F, Samsuddin N, et al.

Int J Infect Dis, 2020 Jan;90:219-222.
PMID: 31682962 DOI: 10.1016/j.ijid.2019.10.037

The emergence of non-vaccine multidrug-resistant Streptococcus pneumoniae serotypes is on rise. This study was performed to investigate a highly resistant serotype 15A S. pneumoniae isolated from the blood specimen of a 20-month-old patient who died of her infection. The SS40_16 isolate was resistant to erythromycin, co-trimoxazole, tetracycline, and chloramphenicol, as well as to penicillin, ceftriaxone, and cefotaxime (using meningitis cut-off points, Clinical and Laboratory Standards Institute). The isolate belonged to sequence type 1591 (ST1591) and was related to CC81 clonal complex, suggesting the possibility of horizontal gene transfer. Scanning electron microscopy comparison between resistant and sensitive pneumococcal isolates also indicated similar phenotypic characteristics that confer high resistance. The emergence of highly resistant non-vaccine pneumococci is of great concern to public health and in the clinical setting. Pneumococcal surveillance programs represent a crucial tool, not only for determining the impact of pneumococcal conjugate vaccines, but also for monitoring the selective pressure of serotype replacement with regard to the treatment of invasive pneumococcal disease.
Rapid and sensitive detection of Salmonella with reduced graphene oxide-carbon nanotube based electrochemical aptasensor

Appaturi JN, Pulingam T, Thong KL, Muniandy S, Ahmad N, Leo BF

Anal Biochem, 2020 01 15;589:113489.
PMID: 31655050 DOI: 10.1016/j.ab.2019.113489

Rapid detection of foodborne pathogens is crucial as ingestion of contaminated food products may endanger human health. Thus, the objective of this study was to develop a biosensor using reduced graphene oxide-carbon nanotubes (rGO-CNT) nanocomposite via the hydrothermal method for accurate and rapid label-free electrochemical detection of pathogenic bacteria such as Salmonella enterica. The rGO-CNT nanocomposite was characterized using Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction and transmission electron microscopy. The nanocomposite was dropped cast on the glassy carbon electrode and further modified with amino-modified DNA aptamer. The resultant ssDNA/rGO-CNT/GCE aptasensor was then used to detect bacteria by using differential pulse voltammetry (DPV) technique. Synergistic effects of aptasensor was evident through the combination of enhanced electrical properties and facile chemical functionality of both rGO and CNT for the stable interface. Under optimal experimental conditions, the aptasensor could detect S. Typhimurium in a wide linear dynamic range from 101 until 108 cfu mL-1 with a 101 cfu mL-1 of the limit of detection. This aptasensor also showed good sensitivity, selectivity and specificity for the detection of microorganisms. Furthermore, we have successfully applied the aptasensor for S. Typhimurium detection in real food samples.
The adaptation towards climate change impacts among islanders in Malaysia

Ahmad N, Shaffril HAM, Abu Samah A, Idris K, Abu Samah B, Hamdan ME

Sci Total Environ, 2020 Jan 10;699:134404.
PMID: 31678877 DOI: 10.1016/j.scitotenv.2019.134404

The climate change phenomenon has been occurring in every part of the world, including Malaysia. In particular, changes such as rising temperature, sea level rise, and unstable rain pattern are proven to affect the socio-economic routine of the community. Hence, it is necessary to learn how to adapt to it, especially those who heavily rely on nature stability. The present study examined the adaptation towards climate change among islanders in Malaysia. In addition, the current research was performed quantitatively using a developed questionnaire as the main data collection tool. In this case, a total of 400 islanders were selected as the respondents through a multi-stage sampling technique. The results revealed that the respondents recorded a moderate to high mean score for adaptation aspects namely awareness, dependency and structure. Accordingly, a number of recommendations that were highlighted can be utilized as a basis to develop community adaptation policy that is in line with the islanders' need, ability, and interests.
Fulltext Hypertension control among euvolemic hypertensive hemodialysis patients in Malaysia: a prospective follow-up study

Khan A, Khan AH, Adnan AS, Sulaiman SAS, Mushtaq S, Ahmad N, et al.

J Pharm Policy Pract, 2019;12:10.
PMID: 31114693 DOI: 10.1186/s40545-019-0169-y

Objectives: Existing literature does not provide enough information on evaluation of factors associated with pre-dialysis controlled hypertension among euvolemic hemodialysis (HD) patients. We conducted a study to evaluate the rate and factors influencing pre-dialysis controlled hypertension among euvolemic HD patients.
Design: A multicenter prospective follow-up study.
Setting: Tertiary care teaching hospital and its associated private dialysis centers.
Participants: This study included 145 euvolemic eligible hypertensive patients. Various sociodemographic, clinical factors and drugs were investigated and analyzed by using appropriate statistical methods to determine the factors influencing hypertension control among the study participants.
Results: On baseline visit, the mean pre-dialysis systolic and diastolic BP (mmHg) of study participants was 161.2 ± 24. and 79.21 ± 11.8 retrospectively, and 30 (20.6%) patients were on pre-dialysis goal BP. At the end of the 6-months follow-up, the mean pre-dialysis systolic BP and diastolic BP (mmHg) of the patients was 154.6 ± 18.3 and 79.2 ± 11.8 respectively, and 42 (28.9%) were on pre-dialysis goal BP. In multivariate analysis, the use of calcium channel blockers (CCBs) was the only variable which had statistically significant association with pre-dialysis controlled hypertension at baseline (OR = 7.530, p-value = 0.001) and final (OR = 8.988, p-value
Peptide Cross-Linked Poly(2-oxazoline) as a Sensor Material for the Detection of Proteases with a Quartz Crystal Microbalance

Ahmad N, Colak B, Gibbs MJ, Zhang DW, Gautrot JE, Watkinson M, et al.

Biomacromolecules, 2019 07 08;20(7):2506-2514.
PMID: 31244015 DOI: 10.1021/acs.biomac.9b00245

Inflammatory conditions are frequently accompanied by increased levels of active proteases, and there is rising interest in methods for their detection to monitor inflammation in a point of care setting. In this work, new sensor materials for disposable single-step protease biosensors based on poly(2-oxazoline) hydrogels cross-linked with a protease-specific cleavable peptide are described. The performance of the sensor material was assessed targeting the detection of matrix metalloproteinase-9 (MMP-9), a protease that has been shown to be an indicator of inflammation in multiple sclerosis and other inflammatory conditions. Films of the hydrogel were formed on gold-coated quartz crystals using thiol-ene click chemistry, and the cross-link density was optimized. The degradation rate of the hydrogel was monitored using a quartz crystal microbalance (QCM) and showed a strong dependence on the MMP-9 concentration. A concentration range of 0-160 nM of MMP-9 was investigated, and a lower limit of detection of 10 nM MMP-9 was determined.
Fulltext Mainstream Preschool Teachers' Skills at Identifying and Referring Children with Autism Spectrum Disorder (ASD)

Mohammed Taresh S, Aniza Ahmad N, Roslan S, Ma'rof AM, Mohammed Zaid S

Int J Environ Res Public Health, 2020 06 16;17(12).
PMID: 32560042 DOI: 10.3390/ijerph17124284

Background: Early intervention will help children with autism spectrum disorder (ASD) to attain early learning reinforcement. This study focuses on exploring the ability of preschool teachers to identify children with ASD and their referral decision-making process. Method: This is a mixed-method study (qualitative and quantitative methods) involving 20 respondents. The qualitative study is based on an open question case study, while the quantitative study consists of questionnaire with demographic variables to identify the effect of the demographic variables on the preschool teachers' ability to identify children with ASD. Sample: The sample was selected via convenience sampling among mainstream preschool teachers. The data was analyzed using SPSS software and thematic analysis. Results: The findings show that preschool teachers did not have skills at identifying children with ASD, and the majority of them labelled children with ASD as spoilt or hyperactive children. They also viewed children with ASD as having other disorders such as attention deficit hyperactivity disorder (ADHD) or communication disorders such as introversion. Moreover, preschool teachers expressed that the reason for the child's behaviour could be due to the parents' inability to properly educate their child. Additionally, the demographic variables of the preschool teachers, such as age, education level and teaching experience, were found not to affect their ASD identification skills. Conclusion: Preschool teachers need to improve their skills in identifying ASD among children via training.
Fulltext Impact of Substantive Staging and Communicative Staging of Sustainable Servicescape on Behavioral Intentions of Hotel Customers through Overall Perceived Image: A Case of Boutique Hotels

Khalil-Ur-Rehman, Adnan M, Ahmad N, Scholz M, Khalique M, Naveed RT, et al.

Int J Environ Res Public Health, 2021 08 30;18(17).
PMID: 34501713 DOI: 10.3390/ijerph18179123

Customers have become very sensitive regarding the innovative evaluation of services. Due to competition in the hospitality industry, it is a challenge for hotel marketers to understand customers' behavior. There is scant research in the hotel industry of Pakistan and especially on boutique hotels. This research seeks to measure the relationship between substantive, communicative elements of the sustainable servicescape and behavioral intentions (word of mouth) in a boutique hotel setting. However, the mediating effect of the overall perceived image is examined between these constructs. Responses of boutique hotel visitors were collected from Lahore, Islamabad, Faisalabad, and Murree. Data were analyzed by using structural equation modeling (SEM). Results display that both substantive and communicative servicescape elements positively affect the perceived image of customers, which has a positive influence on behavioral intentions such as word of mouth (WOM). Theoretical and practical implications are also discussed.
Maggot Debridement Therapy in Malaysia

Nair HKR, Wasi Ahmad N, Teh CH, Lee HL, Chong SSY

Int J Low Extrem Wounds, 2021 Sep;20(3):208-216.
PMID: 32524879 DOI: 10.1177/1534734620932397

Maggot therapy, also known as maggot debridement therapy, larval therapy, biodebridement, or biosurgery, is a type of biotherapy involving the intentional application of live, disinfected fly larvae or maggots into the nonhealing wound of a human or animal to debride the necrotic wound, reduce bacterial contamination of the wound as well as enhance the formation of healthy granulation tissue and stimulate healing in nonhealing wounds. In addition, van der Plas et al reported that the use of the medicinal larvae as natural remover of necrotic and infected tissue had prevented amputation in 11 selected patients. In Malaysia, Aaron et al had demonstrated prevention of amputation in 25 patients.
Psychometric properties of the Malay inventory for the perception of Muslims with hearing impairment

Rahmat S, Yati IES, Musa R, A Rahman S, Ahmad NS, Dzulkarnain AAA

Med J Malaysia, 2021 09;76(5):672-679.
PMID: 34508373

OBJECTIVE: The aims of this study are to measure the psychometric properties of the newly developed preliminary version of hearing impairment inventory for religious duties for Muslim adults, i.e., the Inventori Persepsi Bagi Muslim Yang Memiliki Masalah Pendengaran (IPM3P), and to produce a final version of IPM3P.
METHODS: The preliminary version of IPM3P that is used to investigate the perception of Muslim adults with hearing impairment towards Islamic understanding and practice has been tested in this study. The preliminary version of IPM3P consists of three domains (obligation, practice, and difficulty) with 59 items in total. Four phases of validity and reliability testing involved were: i) Content validation, ii) Pretesting, face validity and proofreading, iii) Pilot study, and iv) Psychometric evaluation.
RESULTS: The final version of IPM3P consists of 36 items. The findings from the present study suggest that the final version of IPM3P has excellent psychometric properties manifested by: i) good content validity, ii) excellently pretested, iii) good face validity, iv) good construct validity shown by principal component analysis and convergent validity, and v) good discriminant validity showed by divergent validity.
CONCLUSION: IPM3P shows good potential to be used as a tool in investigating perception of Muslim adults towards Islamic understanding and practice.
Pharmacological and non-pharmacological management of spinocerebellar ataxia: A systematic review

Yap KH, Azmin S, Che Hamzah J, Ahmad N, van de Warrenburg B, Mohamed Ibrahim N

J Neurol, 2021 Nov 06.
PMID: 34743220 DOI: 10.1007/s00415-021-10874-2

Spinocerebellar ataxias (SCA) comprise a rare, genetic subgroup within the degenerative ataxias and are dominantly inherited, with up to 48 recognized genetic subtypes. While an updated review on the management of degenerative ataxia is published recently, an evidence-based review focussed on the management of SCA is lacking. Here, we reviewed the pharmacological and non-pharmacological management of SCA by conducting a systematic review on Medline Ovid and Scopus. Of 29,284 studies identified, 47 studies (pharmacological: n = 25; non-pharmacological: n = 22) that predominantly involved SCA patients were included. Twenty studies had a high risk of bias based on the Cochrane's Collaboration risk of bias tool. As per the European Federation of Neurological Societies 2004 guideline for therapeutic intervention, the remaining 27 studies were of Class I (n = 4) and Class II (n = 23) evidence. Only two therapies had Level A recommendations for the management of ataxia symptoms: riluzole and immediate in-patient neurorehabilitation. Ten therapies had Level B recommendations for managing ataxia symptoms and require further investigations with better study design. These include high dose valproate acid, branched-chain amino acid, intravenous trehalose; restorative rehabilitation using cycling regimen and videogame; and cerebellar stimulations using transcranial direct current stimulation and transcranial magnetic stimulation. Lithium and coaching on psychological adjustment received Level B recommendation for depressive symptoms and quality of life, respectively. Heterogeneous study designs, different genotypes, and non-standardized clinical measures alongside short duration and small sample sizes may hamper meaningful clinical translation. Therefore, rating of recommendations only serve as points of reference.
Fulltext Nigella sativa fixed and essential oil improves antioxidant status through modulation of antioxidant enzymes and immunity

Sultan MT, Butt MS, Karim R, Ahmad N, Ahmad RS, Ahmad W

Pak J Pharm Sci, 2015 Mar;28(2):589-95.
PMID: 25730812

The onset of 21st century witnessed the awareness among the masses regarding the diet-health linkages. The researchers attempted to explore traditional products/plants were in the domain of pharmacy and nutrition focussing on their health benefits. In the present research intervention, we investigate the role of Nigella sativa fixed oil (NSFO) and essential oil (NSEO) in improving antioxidant status and modulation of enzymes. The National Institute of Health (NIH) provided us 30 Sprague Dawley rats that were equally placed in three groups. The groups were fed on their respective diets (56 days) two experimental diets i.e. D2 (NSFO @ 4.0%) and D3 (NSEO @ 0.30%) and control. The indices pertaining to antioxidant status, antioxidant enzymes, and parameters pertaining to immunity were evaluated at 4 weeks interval. The experimental diets (NSFO@ 4.0% & NSEO@ 0.30%) modulated the activities of antioxidant enzymes i.e., catalase (CAT), superoxide dismutase (SOD), glutathione transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx), positively. Indices of antioxidant status like tocopherols and glutathione were in linear relationship with that of GPx, GR and GST (P<0.01). Myeloperoxidase activities were in negative correlation with GST (P<0.01) but positive correlation with some other parameters. In the nutshell, the fixed and essential oil of Nigella sativa are effective in improving the indices pertaining to antioxidant status, however, the immune boosting potential needs further clarification. However, authors are of the view that there is need to explore the molecular targets of Nigella sativa fixed and essential oils. Findings from such studies would be useful to validate this instant study for health promoting potential against diabetes mellitus and cardiovascular disorders.
Evaluation of superabsorbent linseed-polysaccharides as a novel stimuli-responsive oral sustained release drug delivery system

Haseeb MT, Hussain MA, Bashir S, Ashraf MU, Ahmad N

Drug Dev Ind Pharm, 2017 Mar;43(3):409-420.
PMID: 27808567 DOI: 10.1080/03639045.2016.1257017

CONTEXT: Advancement in technology has transformed the conventional dosage forms to intelligent drug delivery systems. Such systems are helpful for targeted and efficient drug delivery with minimum side effects. Drug release from these systems is governed and controlled by external stimuli (pH, enzymes, ions, glucose, etc.). Polymeric biomaterial having stimuli-responsive properties has opened a new area in drug delivery approach.
OBJECTIVE: Potential of a polysaccharide (rhamnogalacturonan)-based hydrogel from Linseeds (Linum usitatissimum L.) was investigated as an intelligent drug delivery material.
MATERIALS AND METHODS: Different concentrations of Linseed hydrogel (LSH) were used to prepare caffeine and diacerein tablets and further investigated for pH and salt solution-responsive swelling, pH-dependent drug release, and release kinetics. Morphology of tablets was observed using SEM.
RESULTS: LSH tablets exhibited dynamic swelling-deswelling behavior with tendency to swell at pH 7.4 and in deionized water while deswell at pH 1.2, in normal saline and ethanol. Consequently, pH controlled release of the drugs was observed from tablets with lower release (<10%) at pH 1.2 and higher release at pH 6.8 and 7.4. SEM showed elongated channels in swollen then freeze-dried tablets.
DISCUSSION: The drug release was greatly influenced by the amount of LSH in the tablets. Drug release from LSH tablets was governed by the non-Fickian diffusion.
CONCLUSIONS: These finding indicates that LSH holds potential to be developed as sustained release material for tablet.
Advances in solid-state fermentation for bioconversion of agricultural wastes to value-added products: Opportunities and challenges

Chilakamarry CR, Mimi Sakinah AM, Zularisam AW, Sirohi R, Khilji IA, Ahmad N, et al.

Bioresour Technol, 2022 Jan;343:126065.
PMID: 34624472 DOI: 10.1016/j.biortech.2021.126065

The increase in solid waste has become a common problem and causes environmental pollution worldwide. A green approach to valorise solid waste for sustainable development is required. Agricultural residues are considered suitable for conversion into profitable products through solid-state fermentation (SSF). Agricultural wastes have high organic content that is used as potential substrates to produce value-added products through SSF. The importance of process variables used in solid-phase fermentation is described. The applications of SSF developed products in the food industry as flavouring agents, acidifiers, preservatives and flavour enhancers. SSF produces secondary metabolites and essential enzymes. Wastes from agricultural residues are used as bioremediation agents, biofuels and biocontrol agents through microbial processing. In this review paper, the value addition of agricultural wastes by SSF through green processing is discussed with the current knowledge on the scenarios, sustainability opportunities and future directions of a circular economy for solid waste utilisation.
Fulltext Mechanical and Microstructural Characterization of Quarry Rock Dust Incorporated Steel Fiber Reinforced Geopolymer Concrete and Residual Properties after Exposure to Elevated Temperatures

Ibraheem M, Butt F, Waqas RM, Hussain K, Tufail RF, Ahmad N, et al.

Materials (Basel), 2021 Nov 15;14(22).
PMID: 34832298 DOI: 10.3390/ma14226890

The purpose of this research is to study the effects of quarry rock dust (QRD) and steel fibers (SF) inclusion on the fresh, mechanical, and microstructural properties of fly ash (FA) and ground granulated blast furnace slag (SG)-based geopolymer concrete (GPC) exposed to elevated temperatures. Such types of ternary mixes were prepared by blending waste materials from different industries, including QRD, SG, and FA, with alkaline activator solutions. The multiphysical models show that the inclusion of steel fibers and binders can enhance the mechanical properties of GPC. In this study, a total of 18 different mix proportions were designed with different proportions of QRD (0%, 5%, 10%, 15%, and 20%) and steel fibers (0.75% and 1.5%). The slag was replaced by different proportions of QRD in fly ash, and SG-based GPC mixes to study the effect of QRD incorporation. The mechanical properties of specimens, i.e., compressive strength, splitting tensile strength, and flexural strength, were determined by testing cubes, cylinders, and prisms, respectively, at different ages (7, 28, and 56 days). The specimens were also heated up to 800 °C to evaluate the resistance of specimens to elevated temperature in terms of residual compressive strength and weight loss. The test results showed that the mechanical strength of GPC mixes (without steel fibers) increased by 6-11%, with an increase in QRD content up to 15% at the age of 28 days. In contrast, more than 15% of QRD contents resulted in decreasing the mechanical strength properties. Incorporating steel fibers in a fraction of 0.75% by volume increased the compressive, tensile, and flexural strength of GPC mixes by 15%, 23%, and 34%, respectively. However, further addition of steel fibers at 1.5% by volume lowered the mechanical strength properties. The optimal mixture of QRD incorporated FA-SG-based GPC (QFS-GPC) was observed with 15% QRD and 0.75% steel fibers contents considering the performance in workability and mechanical properties. The results also showed that under elevated temperatures up to 800 °C, the weight loss of QFS-GPC specimens persistently increased with a consistent decrease in the residual compressive strength for increasing QRD content and temperature. Furthermore, the microstructure characterization of QRD blended GPC mixes were also carried out by performing scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive spectroscopy (EDS).
Genome Sequences of Brucella melitensis, Isolated from Blood Samples of Brucellosis Patients in Malaysia

Mohamed Zahidi J, Ahmad N, Tay BY, Hashim R, Khoo E, Ahmad N, et al.

Genome Announc, 2017 Aug 03;5(31).
PMID: 28774972 DOI: 10.1128/genomeA.00689-17

Human brucellosis is a neglected zoonotic disease and has widespread geographical distribution. Brucella melitensis has caused outbreaks and sporadic cases in Malaysia. Here, we present the whole-genome sequences of four B. melitensis strains isolated from brucellosis patients in Malaysia.
Dental students' perceptions on the contribution and impact role of a clinical teacher

Shoaib LA, Safii SH, Naimie Z, Ahmad NA, Sukumaran P, Yunus RM

Eur J Dent Educ, 2018 Feb;22(1):e26-e34.
PMID: 27995730 DOI: 10.1111/eje.12252

OBJECTIVES: This study was conducted in University of Malaya to evaluate student perceptions on the contribution and role of an effective clinical teacher based on the cognitive apprenticeship model in clinical practice.
METHODS: Self-administered questionnaires were distributed to 233 undergraduate dental students involved with clinical teaching. This modified and validated questionnaire focusing on students' learning environment was used in order to gain relevant information related to dental clinical teaching. Six domains with different criteria applicable to clinical teaching in dentistry were selected consisting of modelling (four criteria), coaching (four criteria), scaffolding (four criteria), articulation (four criteria), reflection (two criteria) and general learning environment (six criteria). Data analyses were performed using IBM SPSS Statistics 20.
RESULTS: Majority of the students expressed positive perceptions on their clinical learning experience towards the clinical teachers in the Faculty of Dentistry, University of Malaya, in all criteria of the domains. Few negative feedbacks concerning the general learning environment were reported.
CONCLUSION: Further improvement in the delivery of clinical teaching preferably by using wide variety of teaching-learning activities can be taken into account through students' feedback on their learning experience.
Optically stimulated Al2O3:C luminescence dosimeters for teletherapy: Hp(10) performance evaluation

Hashim S, Musa Y, Ghoshal SK, Ahmad NE, Hashim IH, Yusop M, et al.

Appl Radiat Isot, 2018 May;135:7-11.
PMID: 29353759 DOI: 10.1016/j.apradiso.2018.01.010

The performance of optically stimulated luminescence dosimeters (OSLDs, Al2O3:C) was evaluated in terms of the operational quantity of HP(10) in Co-60 external beam teletherapy unit. The reproducibility, signal depletion, and dose linearity of each dosimeter was investigated. For ten repeated readouts, each dosimeter exposed to 50mSv was found to be reproducible below 1.9 ± 3% from the mean value, indicating good reader stability. Meanwhile, an average signal reduction of 0.5% per readout was found. The dose response revealed a good linearity within the dose range of 5-50mSv having nearly perfect regression line with R2 equals 0.9992. The accuracy of the measured doses were evaluated in terms of operational quantity HP(10), wherein the trumpet curve method was used respecting the 1990 International Commission on Radiological Protection (ICRP) standard. The accuracy of the overall measurements from all dosimeters was discerned to be within the trumpet curve and devoid of outlier. It is established that the achieved OSL Al2O3:C dosimeters are greatly reliable for equivalent dose assessment.
Fulltext A Fatal Case of Candida auris and Candida tropicalis Candidemia in Neutropenic Patient

Mohd Tap R, Lim TC, Kamarudin NA, Ginsapu SJ, Abd Razak MF, Ahmad N, et al.

Mycopathologia, 2018 Jun;183(3):559-564.
PMID: 29383574 DOI: 10.1007/s11046-018-0244-y

We report a fatal case of Candida auris that was involved in mixed candidemia with Candida tropicalis, isolated from the blood of a neutropenic patient. Identification of both isolates was confirmed by amplification and sequencing of internal transcribed spacer and D1/D2 domain of large subunit in rRNA gene. Antifungal susceptibility test by E-test method revealed that C. auris was resistant to amphotericin B, anidulafungin, caspofungin, fluconazole, itraconazole and voriconazole. On the other hand, C. tropicalis was sensitive to all antifungal tested. The use of chromogenic agar as isolation media is vital in detecting mixed candidemia.
Fulltext Prevalence of Antibiotic-Resistant Bacteria and Antibiotic-Resistant Genes and the Quantification of Antibiotics in Drinking Water Treatment Plants of Malaysia: Protocol for a Cross-sectional Study

Mohamad ZA, Bakon SK, Jamilan MAJ, Daud N, Ciric L, Ahmad N, et al.

JMIR Res Protoc, 2022 Nov 21;11(11):e37663.
PMID: 36409546 DOI: 10.2196/37663

BACKGROUND: Antimicrobial resistance is a known global public health threat. In addition, it brings serious economic consequences to agriculture. Antibiotic resistance in humans, animals, and environment is interconnected, as proposed in the tricycle surveillance by the World Health Organization. In Malaysia, research and surveillance of antimicrobial resistance are mainly performed in clinical samples, agricultural settings, and surface waters, but no surveillance of the drinking water systems has been performed yet. Hence, this policy-driven study is a combined effort of microbiologists and engineers to provide baseline data on the magnitude of antimicrobial resistance in the drinking water systems of Malaysia.
OBJECTIVE: The aim of this study was to study the baseline level of antibiotic-resistant bacteria in the drinking water distribution systems of Malaysia by collecting samples from the pretreatment and posttreatment outlets of water treatment plants in a selected state of Malaysia. We aimed to determine the prevalence of antibiotic-resistant bacteria, the occurrence of antibiotic-resistant genes, and the level of antibiotics present in the drinking water systems.
METHODS: This is a laboratory-based, cross-sectional study in a selected state of Malaysia. Water samples from 6 drinking water treatment plants were collected. Samples were collected at 3 sampling points, that is, the intake sampling station, service reservoir outlet station, and the distribution system sampling station. These were tested against 7 types of antibiotics in triplicates. Samples were screened for antibiotic-resistant bacteria and antibiotic-resistant genes and quantified for the level of antibiotics present in the drinking water treatment plants.
RESULTS: We will show the descriptive statistics of the number of bacterial colonies harvested from water samples grown on Reasoner's 2A agar with or without antibiotics, the occurrence of antibiotic-resistant genes, and the level of antibiotics detected in the water samples. The sampling frame was scheduled to start from November 2021 and continue until December 2022. Data analysis is expected to be completed by early 2023, and the results are expected to be published in mid-2023.
CONCLUSIONS: This study provides baseline information on the status of the antimicrobial-resistant bacteria, the presence of resistance genes as contaminants, and the level of antibiotics present in the drinking water systems of Malaysia, with the aim of demonstrating to policymakers the need to consider antimicrobial resistance as a parameter in drinking water surveillance.
INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): DERR1-10.2196/37663.
Fulltext Theory-based immunisation health education intervention in improving child immunisation uptake among antenatal mothers attending federal medical centre in Nigeria: A study protocol for a randomized controlled trial

Galadima AN, Mohd Zulkefli NA, Said SM, Ahmad N, Garba SN

PLoS One, 2022;17(12):e0263436.
PMID: 36480545 DOI: 10.1371/journal.pone.0263436

BACKGROUND: Childhood immunisation coverage is very low in Nigeria (31%) with Zamfara State being amongst the states with the poorest coverage (<10%). Lack of maternal knowledge, attitude, outcome expectations, self-efficacy, cultural beliefs and assumptions of religious regulations of antenatal mothers towards childhood immunisation are the contributory factors to poor childhood immunisation uptake. This study aims is to develop, implement and evaluate the effects of an immunisation health educational intervention with application of Social Cognitive Theory on pregnant women to improve knowledge, attitude, outcome expectations, self-efficacy, cultural beliefs and assumptions on religious regulations regarding childhood immunization uptake in Federal Medical Centre Gusau, Zamfara State, Nigeria.
METHODOLOGY: The study will be a single-blind parallel-group randomised controlled trial, where baseline data will be collected from 392 estimated antenatal mothers, after that they will be evenly randomised using randomly generated permuted block sizes (each containing two intervention and two control assignments). The study participants will be antenatal mothers of ages 18 years and above who are in third trimesters and attending Federal Medical Centre Gusau, Zamfara State, Nigeria; during the study period and fulfilled all the inclusion and exclusion criteria. The intervention group will undergo five-health education sessions on immunisation, which will be strictly guided by Social Cognitive Theory-based intervention module: while the control group will receive usual care (standard care). Follow-up data will be collected using the same questionnaire at 6-weeks post-delivery, 10-weeks post-delivery and 14-weeks post-delivery. The generalized linear mixed model will be carried-out to determine the overall effect of the intervention after controlling for 14 potential confounding variables. An intention to treat analysis will also be carried-out. Childhood immunisation uptake is the primary outcome while the secondary outcomes are: improved knowledge scores, attitude scores, outcomes expectation, self-efficacy scores, cultural beliefs scores and assumptions on religious regulations scores.
DISCUSSION: The study will be a randomised controlled trial, that focuses on the effects of an immunisation health educational intervention with application of Social Cognitive Theory on pregnant women to improve knowledge, attitude, outcome expectations, self-efficacy, cultural beliefs and assumptions on religious regulations regarding childhood immunisation uptake in Federal Medical Centre Gusau, Zamfara State, Nigeria.
TRIAL REGISTRATION: Pan African Clinical Trial Registry PACTR202006722055635. Protocol registered on 09 June 2020.

Filters

Please provide feedback to Administrator ([email protected])

External Links