This study was undertaken to analyze the efficiency of Botryococcus sp. in the phycoremediation of domestic wastewater and to determine the variety of hydrocarbons derived from microalgal oil after phycoremediation. The study showed a significant (p < 0.05) reduction of pollutant loads of up to 93.9% chemical oxygen demand, 69.1% biochemical oxygen demand, 59.9% total nitrogen, 54.5% total organic carbon, and 36.8% phosphate. The average dry weight biomass produce was 0.1 g/L of wastewater. In addition, the dry weight biomass of Botryococcus sp. was found to contain 72.5% of crude oil. The composition analysis using Gas Chromatogram - Mass Spectrometry (GC-MS) found that phthalic acid, 2-ethylhexyltridecyl ester (C29H48O4), contributed the highest percentage (71.6%) of the total hydrocarbon compounds to the extracted algae oil. The result of the study suggests that Botryococcus sp. can be used for effective phycoremediation, as well as to provide a sustainable hydrocarbon source as a value-added chemical for the bio-based plastic industry.
Microalgae cultivation is well known as a sustainable method for eco-friendly wastewater phycoremediation and valuable biomass production. This study investigates the feasibility and kinetic removal of organic compounds and nutrients from food processing wastewater (FPW) using Botryococcus sp. in an enclosed photobioreactor. Simultaneously, response surface methodology (RSM) via face-centered central composite design (FCCCD) was applied to optimize the effects of alum and chitosan dosage and pH sensitivity on flocculation efficiency. The maximum growth rate of Botryococcus sp. cultivated in FPW was 1.83 mg day-1with the highest removal of chemical oxygen demand (COD), total organic carbon (TOC), and total phosphorus (TP) after 12 days of phycoremediation of 96.1%, 87.2%, and 35.4%, respectively. A second-order polynomial function fits well with the experimental results. Both coagulant dosage and pH significantly (p