Bauxite mining activities in Felda Bukit Goh, Kuantan, has been in operation since early 2013. The uncontrolled min- ing activities created dusty environment which led to health complaints from the surrounding residence. The mining activities had also caused anxiety and stress among the community due the noise which disturb their well-being and their sleep (1). The objectives of this study were to determine and relate selected heavy metal levels (As, Cd, Cr, Ni, Pb and Al) in the dust in the classrooms of a school located near to the mines with the respiratory health symptoms reported by the parents of the selected school children. A cross-sectional study was conducted on 121 students randomly selected from the Primary 4 and 5 clusters. Questionnaires were used to collect information on their back- ground and their respiratory health symptoms. Environmental sampling for 16 dust samples consisted two samples (window and corridors) from each of the eight classes (four from each Primary 4 and 5) were collected using 400W vacuum cleaner with special filter from these classrooms. The dust samples were analyzed using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Results showed that the levels of heavy metal in the dust were high in both the Primary 5 and Primary 4 classes. The mean heavy metal levels in the dust from the classroom area varied signifi- cantly and decreased in the order of: Al > Cr > Ni > Pb > As > Cd. The mean ranges were as follows: Al (39360.15- 110824.1 mg/kg); Cr (2364.84- 4726.009 mg/kg); Ni (699.9606-1443.36 mg/kg); Pb (434.3067- 1650.76 mg/kg); As
(200.7891-454.7241 mg/kg); and Cd (10.9107-34.9902 mg/kg) respectively. The levels were higher at the corridor than the window. Nasal congestion (45.5%) was the highest respiratory symptoms experienced by the school chil- dren. There was a significant correlation between the respiratory health symptoms reported with absenteeism in the year 2015 when the mining activities were at the peak. There was a significant correlation between dust Pb levels with respiratory reported symptoms of the school children. Other risk factors which contributed to the respiratory symptoms reported were the close location of their houses to the mining areas, young age and low family income.
A quantitative assay using high-performance thin-layer chromatography (HPTLC) was developed to investigate bile salt hydrolase (BSH) activity in Pediococcus pentosaceus LAB6 and Lactobacillus plantarum LAB12 probiotic bacteria isolated from Malaysian fermented food. Lactic acid bacteria (LAB) were cultured in de Man Rogosa and Sharpe (MRS) broth containing 1 mmol/L of sodium-based glyco- and tauro-conjugated bile salts for 24 h. The cultures were centrifuged and the resultant cell free supernatant was subjected to chromatographic separation on a HPTLC plate. Conjugated bile salts were quantified by densitometric scans at 550 nm and results were compared to digital image analysis of chromatographic plates after derivatisation with anisaldehyde/sulfuric acid. Standard curves for bile salts determination with both methods show good linearity with high coefficient of determination (R2) between 0.97 and 0.99. Method validation indicates good sensitivity with low relative standard deviation (RSD) (<10%), low limits of detection (LOD) of 0.4 versus 0.2 μg and limit of quantification (LOQ) of 1.4 versus 0.7 μg, for densitometric vs digital image analysis method, respectively. The bile salt hydrolase activity was found to be higher against glyco- than tauro-conjugated bile salts (LAB6; 100% vs >38%: LAB12; 100% vs >75%). The present findings strongly show that quantitative analysis via digitally-enhanced HPTLC offers a rapid quantitative analysis for deconjugation of bile salts by probiotics.
Patients with diabetes have an earlier onset and increased severity of anaemia compared to those with similar degree of renal impairment from other causes. Anaemia is associated with an increased risk of vascular complications. In this study, we determined the prevalence of anaemia in T2DM patients and its association with sociodemographic, clinical and laboratory parameters in an endocrine tertiary hospital in Malaysia. This was a cross-sectional study using retrospective electronic data from January 2011 to December 2013 of 165 T2DM patients in Hospital Putrajaya. Data was analysed using IBM SPSS Statistics version 21.0 for Windows. The prevalence of anaemia was 39.4% and majority had normocytic normochromic (80%), mild (58.5%) anaemia. Majority were Malays (73.9%), aged below 60 with comparable gender percentage and long-standing, poorly-controlled DM [median fasting blood sugar (FBS) 8mmol/L; glycated haemoglobin (HbA1c) 7.9%]. Using the KDIGO chronic kidney disease (CKD) staging system, 86% of these patients were in stages 3-5. Anaemic patients had a significantly higher serum urea, creatinine and a lower FBS, estimated glomerular filtration rate (eGFR) compared to non-anaemic patients. Anaemic patients with diabetic nephropathy had a significantly lower haemoglobin (Hb) compared to those without this complication (p=0.022). The sensitivity and specificity at a cut-off eGFR value of 38.3 ml/min/1.73 m2 (maximum Youden index = 0.462) was 66.7% and 79.5%, respectively to discriminate mild from moderate anaemia. This study shows that anaemia is already present in T2DM patients in Hospital Putrajaya at initial presentation to the specialist outpatient clinic and is significantly associated with CKD. Hence, it emphasises the obligatory need for routine and follow-up full blood count monitoring in T2DM patients in primary care as well as tertiary settings in Malaysia to enable early detection and aggressive correction of anaemia in preventing further complications.