The light train (LRT) is one of the important public transportation modes in the congested city of Kuala Lumpur. Train drivers drive the trains within their shift time on similar routes every day. This job activity may lead to stress. Physical fatigue is one of the health problems commonly experienced by the train drivers. There were three methods applied in this study, namely, Nordic Questionnaire, NASA-TLX and observations. There were a total of 52 respondents, consisting of the train drivers from Rapid Rail Sdn Bhd, a subsidiary company of Prasarana Malaysia Berhad. The questionnaires were analyzed using Statistical Software for the Social Science (SPSS) with a suitable statistical analysis. It was found that, the train drivers experienced pain on their necks, shoulders and lower backs. The main causes of physical fatigue that affect the performance of a train driver are sleep disorder and uncomfortable seating. Moreover, other effects of physical fatigue also prevent the drivers from carrying out activities at home. The industry has improved the drivers’ workstations such as their seats to reduce the rate of physical fatigue. However, more improvements are expected to be done.