Journal of Sports Science and Physical Education 7(1): Received: 10 January 2018, Accepted: 15 February 2018 – The purpose of this study was to investigate the muscle activation of the quadriceps and the hamstrings muscle during forward lunge. The study was performed using wireless electromyography (EMG) sensor placed on the interest muscles. The parameter used was the root mean square value during forward lunges activity. The results demonstrated that the rectus femoris muscle was more dominant than the biceps femoris muscle during the initial movement when the knee was in flexion direction and the feet was in dorsiflexion. A linear relationship was initiated between the raw data and RMS value of the EMG signal of the rectus femoris and biceps femoris. The motions elicit eccentric activations of the hamstring muscle groups during lunge activity.