MyMedR

Fulltext The isolation of Salmonella from raw, cooked and dried foods

Lim YS, Khor SY, Jegathesan M, Kang SH

Med J Malaysia, 1984 Sep;39(3):220-4.

Between June 1977 and May 1982, 2,291 samples of raw, cooked and dried foods were examined for the presence of Salmonella. Of these samples, 43 were positive, isolations being made from raw foods (4.8%) and cooked foods (0.4%) but not from dried foods. 14 Salmonella seratypes were isolated, Salmonella anatum being the most predominant. The significance of these isolations is discussed and the need for consumer education to reduce the incidence of human salmonellosis is emphasised.

Matched MeSH terms: Salmonella/isolation & purification*

Prevalence of Salmonella in broilers at retail outlets, processing plants and farms in Malaysia

Rusul G, Khair J, Radu S, Cheah CT, Yassin RM

Int J Food Microbiol, 1996 Dec;33(2-3):183-94.

PMID: 8930704

A study was conducted to estimate the prevalence of Salmonella among broilers retailed at wet-markets and processing plants. Litter and feed samples obtained from both broiler and breeder farms were also examined for Salmonella. A total of 158 out of 445 (35.5%) and 52 out of 104 (50.0%) broiler carcasses obtained from wet-markets and processing plants were contaminated with Salmonella, respectively. Salmonella was isolated from 14 out of 98 (14.3%) samples of intestinal content. Litter samples from broiler and breeder farms were positive for Salmonella, 8/40 (20%) and 2/10 (20%), respectively. Salmonella isolates (230) belonging to 15 different serovars were isolated. Predominant serovars were S. enteritidis, S. muenchen, S. kentucky and S. blockley.

Matched MeSH terms: Salmonella/isolation & purification*

Fulltext The occurrence of enteric bacteria on lettuce leaves sold in local markets in Penang, Malaysia

Chapman SJ

Med J Malaysia, 1980 Sep;35(1):7-8.

PMID: 7254003

A survey of lettuce sold in Penang markets showed them to be heavily contaminated with faecal coliforms and nearly half the samples were positive for Salmonella or Shigella. The use of night soil on these vegetables is a likely cause of gastroenteritis.

Matched MeSH terms: Salmonella/isolation & purification*

Fulltext Salmonella in the soil of kidergarten playgrounds in the Klang District

Jegathesan M, Rampal L, Lim YS

Med J Malaysia, 1983 Dec;38(4):308-10.

PMID: 6599988

A survey on the incidence of Salmonellae in soil was conducted on 12 kindergartens in the Klang District, The organism. was isolated from five (three urban and two rural) kindergartens from one or more soil samples tested. Ten isolates comprising six serotypes, namely, Salmonella bareilly, S. haifa, S. abony, S. weltevreden, S. agona and S. stanley, were encountered. The possible role that these soil isolates may play in the transmission. of salmonellae is discussed. The need to use more than one media in the detection of salmonellae is emphasised.

Matched MeSH terms: Salmonella/isolation & purification*

Animal Salmonella surveillance in Peninsular Malaysia, 1981-1985

Joseph PG, Sivanandan SP, Yee HT

Epidemiol Infect, 1988 Jun;100(3):351-9.

PMID: 3378581

During the 5-year (1981-5) surveillance period, 2322 salmonella isolations were recorded from animals and other non-human sources in Peninsular Malaysia. This was an increase of 356% over the preceding 5-year period. The 83 serotypes isolated were recovered from 41 sources. Of these 34 were new serotypes bringing the total number of serotypes isolated from non-human sources to date up 97. Food animals and edible animal products accounted for 92.2% of the total isolations, with cattle and beef accounting for 70% of the total. Salmonella dublin was the most frequently isolated serotype, whereas S. typhimurium had the widest zoological distribution. More than 80% of the non-human salmonella serotypes have also been reported in man in this country.

Matched MeSH terms: Salmonella/isolation & purification*

Fulltext Isolation of Campylobacter and Salmonella from houseflies (Musca domestica) in a university campus and a poultry farm in Selangor, Malaysia

Choo LC, Saleha AA, Wai SS, Fauziah N

Trop Biomed, 2011 Apr;28(1):16-20.

PMID: 21602764 MyJurnal

Insects, in particular house flies and cockroaches, have been shown to be associated with the spread of pathogens in livestock farms and in human disease outbreaks: among these pathogens are salmonellae and campylobacters. A total of 60 flies were caught in three locations: an animal teaching facility and a cafeteria in a university campus, and a poultry farm. Five percent (5%) and 13.3% of flies sampled were found to carry Campylobacter and Salmonella, respectively.

Matched MeSH terms: Salmonella/isolation & purification*

Studies of bacterial disease in West Malaysian. Orang Asli (aborigines): previously unrecorded Salmonella Serotypes

Anandan J, Lim TW, Haug NL

Med J Malaya, 1969 Jun;23(4):269-71.

PMID: 4242174

Matched MeSH terms: Salmonella/isolation & purification*

Salmonella isolation experience in the Pacific Basin region: a survey

Haddock RL

Southeast Asian J Trop Med Public Health, 1983 Sep;14(3):399-402.

PMID: 6658509

To determine if the unusually high incidence of salmonellosis reported on Guam for several years might be the result of more frequent bacteriologic examination of gastroenteritis/diarrhoea patient stool specimens, a survey of medical clinic and laboratory activities was undertaken among countries in the Pacific Basin Area. Survey results suggest that while Guam laboratories may be particularly proficient in isolating Salmonella organisms, the quantity of stool specimens examined could not account for the higher incidence of salmonellosis observed on the island.

Matched MeSH terms: Salmonella/isolation & purification*

Fulltext Isolation and identification of salmonella from curry samples and its sensitivity to commercial antibiotics and aqueous extracts of Camelia sinensis (L.) and Trachyspermum ammi (L.)

Gunasegaran T, Rathinam X, Kasi M, Sathasivam K, Sreenivasan S, Subramaniam S

Asian Pac J Trop Biomed, 2011 Aug;1(4):266-9.

PMID: 23569772 DOI: 10.1016/S2221-1691(11)60040-3

To isolate Salmonella from curry samples and to evaluate the drug sensitivity of the food-borne Salmonella and its susceptibility to specific plant extracts.

Matched MeSH terms: Salmonella/isolation & purification

Fulltext Salmonella: the pelvic masquerader

Valayatham V

Int J Infect Dis, 2009 Mar;13(2):e53-5.

PMID: 18829361 DOI: 10.1016/j.ijid.2008.06.015

Salmonella sp is a significant cause of morbidity and mortality. Although commonly infecting the gastrointestinal system, other presentations are not unheard of. Salmonella is an unlikely and an unusual cause of genital tract infection. We describe a woman with suspected pelvic inflammatory disease eventually confirmed as Salmonella O C2 infection.

Matched MeSH terms: Salmonella/isolation & purification

Satay and Salmonella and listeria infection

Arumugaswamy RK, Ali GR, ab Hamid SN

Lancet, 1993 Jul 24;342(8865):247.

PMID: 8100972

Matched MeSH terms: Salmonella/isolation & purification*

Fulltext Bacterial pathogens from the intestinal tracts of various species of cockroaches

Rampal L, Oothuman P, Jeffery J, Daud MZ, Shekhar C, Senan P, et al.

Med J Malaysia, 1983 Jun;38(2):104-7.

PMID: 6353184

Bacterial isolates were made from the intestinal tracts ofcarious species of cockroaches (Periplaneta americana, Periplaneta brunnea, Periplaneta australasiae, Neostylopyga rhombifolia, Nauphoeta cinerea) trapped from kitchens and stores (houses and hospital), Shigello, flexneri, Salmonella typhi, Escherichia coli and Salmonella sp. were some of the bacteria isolated and identified.

Matched MeSH terms: Salmonella/isolation & purification

Salmonella serotypes isolated from man in Malaysia over the 10-year period 1973-1982

Jegathesan M

J Hyg (Lond), 1984 Jun;92(3):395-9.

PMID: 6376627

The results of serotyping of 10 953 salmonella isolates from humans over a 10-year period, 1973-82 at the Bacteriology Division, Institute for Medical Research, Malaysia are presented. A total of 104 serotypes from 22 'O' groups were encountered; 95 isolates were considered untypable. The three most predominant serotypes, namely Salmonella typhi, S. typhimurium and S. weltevreden together accounted for 54.1% of all isolates whilst the 25 most frequent serotypes accounted for 93.6% of the total. Whilst the commoner serotypes occurred regularly throughout the study period, the rarer ones tended to appear only in one year, when they might be associated with an outbreak, and never again. The pattern of serotypes, though quite similar to the one seen in neighbouring Singapore, is different from those experienced in other places such as Hong Kong, Jakarta, Bangladesh and Manchester.

Matched MeSH terms: Salmonella/isolation & purification

Fulltext Isolation of salmonella from unusual sites in the body

Jegathesan M

Med J Malaysia, 1984 Dec;39(4):289-91.

PMID: 6544935

During the period 1971-1982, 57 salmonella strains other than S. typhi and S. paratyphi were isolated from sites other than stools and blood. Cerebrospinal fluid was the commonest specimen followed by urine and pus. The commonest serotypes of the 22 encountered were S. typhimurium and S. enteritidis. Twenty-four of the 57 patients were children under one year of age. These findings highlight the need to remember the unusual manifestation of salmonellosis in differential diagnoses.

Matched MeSH terms: Salmonella/isolation & purification*

Fulltext Salmonella in native "village" chickens (Gallus domesticus): prevalence and risk factors from farms in South-Central Peninsular Malaysia

Jajere SM, Hassan L, Abdul Aziz S, Zakaria Z, Abu J, Nordin F, et al.

Poult Sci, 2019 Nov 01;98(11):5961-5970.

PMID: 31392329 DOI: 10.3382/ps/pez392

Village chicken or Ayam Kampung, common to Southeast Asian countries, has always been regarded as superior in comparison to commercial broiler chicken in terms of wholesomeness and health benefits. The current study investigates the prevalence and risk factors of Salmonella among village chicken flocks from the central and southern states of Peninsular Malaysia. A total of 35 village flocks were sampled from Selangor (n = 19), Melaka (n = 10), Johor (n = 4), and Negeri Sembilan (n = 2). In total, 1,042 samples were collected; these included cloacal swabs (n = 675), eggs (n = 62), pooled drinking water (n = 175), pooled feeds (n = 70), and pooled flies (n = 60). Isolation of Salmonella from cloacal swabs, poultry drinking water, and feeds was carried out according to the protocols and recommendations of the World Organization for Animal Health (OIE) terrestrial manual. The prevalence of Salmonella at an individual bird-level was 2.5% (17/675, 95% CI: 1.6 to 4.0). All eggs screened were negative; in the case of environmental samples, however, Salmonella was detected in 5.14% (9/175), 7.14% (5/70), and 5.0% (3/60) for water, feed, and flies, respectively. A total of 34 isolates and 8 Salmonella serotypes were identified. Weltevreden (20.6%) was the most common, followed by Typhimurium and Agona (17.6%), Albany and Enteritidis (8.8%), Molade (5.9%), Corvallis and Schleissheim (2.9%), and others grouped as Salmonella spp. (11.8%). Multivariable logistic regression models revealed that Salmonella positivity among flocks could be strongly predicted by storage of feeds (uncovered feeds; OR = 10.38; 95% CI: 1.25 to 86.39; p = 0.030) and uncovered water tanks (uncovered tank; OR = 6.43; 95% CI: 1.02 to 40.60; p = 0.048). The presence of Salmonella in village chickens in the study area was lower than that of commercial chickens in Malaysia.

Matched MeSH terms: Salmonella/isolation & purification*

Animal salmonellosis in peninsular Malaysia. II. Annual and zoological distribution of Salmonella serotypes over the 10-year period 1966-1975

Joseph PG, Anwar M, Jegathesan M

Am J Trop Med Hyg, 1978 May;27(3):562-6.

PMID: 354419

A total of 860 Salmonella isolations were made in Peninsular Malaysia from 15 animal species (domestic and wild), eggs, molluscs, flies, and animal feed. The isolations were distributed among 31 serotypes in eight groups. The most common serotype isolated was Salmonella pullorum, followed by S. choleraesuis and S. infantis. S. typhimurium had the widest zoological distribution. The importance of controlling animal salmonellosis is emphasized.

Matched MeSH terms: Salmonella/isolation & purification

Bacterial enteropathogens in Malaysian shellfish

Jegathesan M, Wah LT, Soon LE, Su Har D, Boo Liat L

Trop Geogr Med, 1976 Jun;28(2):91-5.

PMID: 788266

Three species of commonly eaten shellfish found in Malaysian coastal waters were examined for the presence of common bacterial enteropathogens. Vibrio parahaemolyticus, non-agglutinating vibrios, and various serotypes of enteropathogenic E. coli were isolated from a large proportion of them. Salmonella were isolated in two instances. High colony counts with evidence of faecal contamination indicated the strong possibility of pulltion being the cause for the presence of these enteropathogens. Methods of cooking and eating these shellfish enhance their likelihood of acting as vehicles of diarrhoeal disease.

Matched MeSH terms: Salmonella/isolation & purification*

Isolation and identification of bacterial populations of zoonotic importance from captive non-venomous snakes in Malaysia

Abba Y, Ilyasu YM, Noordin MM

Microb Pathog, 2017 Jul;108:49-54.

PMID: 28478198 DOI: 10.1016/j.micpath.2017.04.038

AIM: Captivity of non-venomous snakes such as python and boa are common in zoos, aquariums and as pets in households. Poor captivity conditions expose these reptiles to numerous pathogens which may result in disease conditions. The purpose of this study was to investigate the common bacteria isolated from necropsied captive snakes in Malaysia over a five year period.

MATERIALS AND METHODS: A total of 27 snake carcasses presented for necropsy at the Universiti Putra Malaysia (UPM) were used in this survey. Samples were aseptically obtained at necropsy from different organs/tissues (lung, liver, heart, kindey, oesophagus, lymph node, stomach, spinal cord, spleen, intestine) and cultured onto 5% blood and McConkey agar, respectively. Gram staining, morphological evaluation and biochemical test such as oxidase, catalase and coagulase were used to tentatively identify the presumptive bacterial isolates.

RESULTS: Pythons had the highest number of cases (81.3%) followed by anaconda (14.8%) and boa (3.7%). Mixed infection accounted for 81.5% in all snakes and was highest in pythons (63%). However, single infection was only observed in pythons (18.5%). A total of 82.7%, 95.4% and 100% of the bacterial isolates from python, anaconda and boa, respectively were gram negative. Aeromonas spp was the most frequently isolated bacteria in pythons and anaconda with incidences of 25 (18%) and 8 (36.6%) with no difference (p > 0.05) in incidence, respectively, while Salmonella spp was the most frequently isolated in boa and significantly higher (p

Matched MeSH terms: Salmonella/isolation & purification

Development of rapid gold nanoparticles based lateral flow assays for simultaneous detection of Shigella and Salmonella genera

Yahaya ML, Zakaria ND, Noordin R, Abdul Razak K

Biotechnol Appl Biochem, 2021 Oct;68(5):1095-1106.

PMID: 32935878 DOI: 10.1002/bab.2029

Salmonella and Shigella genera are common pathogens that contaminate foods and beverages. Lateral flow assays (LFA) are commonly used to detect these pathogens. However, most of the developed LFAs are for single detection. Simultaneous detection of pathogens is required to reduce cost and time. In this work, 40 nm gold nanoparticles (AuNPs) were synthesized using the seeding growth method as labeling agent. The AuNPs were characterized and conjugated with mouse anti-Gram negative endotoxin antibody. The nitrocellulose membrane HF135 was immobilized with anti-mouse IgG antibody as a control line and two separate test lines with either anti-Shigella or anti-Salmonella antibody, respectively. Color intensity of test lines was observed for positive samples. A milk sample was used as proof of concept to mimic actual contamination. The limit of detection of the LFA was 3.0 × 106 CFU/mL for multiplex detection of Shigella flexneri and Salmonella Typhi and for both single detections. The result was comparable with the enzyme-linked immunosorbent assay (ELISA) analysis. The produced LFA could differentiate between Shigella flexneri, Shigella boydii, Salmonella Enteritidis, and Salmonella Typhi. The developed LFA was able to identify Shigella flexneri and Salmonella Typhi with good sensitivity in milk samples, thus, beneficial to ensure the safety of food before entering the market.

Matched MeSH terms: Salmonella/isolation & purification*

Fulltext A portable automatic endpoint detection system for amplicons of loop mediated isothermal amplification on microfluidic compact disk platform

Uddin SM, Ibrahim F, Sayad AA, Thiha A, Pei KX, Mohktar MS, et al.

Sensors (Basel), 2015 Mar 05;15(3):5376-89.

PMID: 25751077 DOI: 10.3390/s150305376

In recent years, many improvements have been made in foodborne pathogen detection methods to reduce the impact of food contamination. Several rapid methods have been developed with biosensor devices to improve the way of performing pathogen detection. This paper presents an automated endpoint detection system for amplicons generated by loop mediated isothermal amplification (LAMP) on a microfluidic compact disk platform. The developed detection system utilizes a monochromatic ultraviolet (UV) emitter for excitation of fluorescent labeled LAMP amplicons and a color sensor to detect the emitted florescence from target. Then it processes the sensor output and displays the detection results on liquid crystal display (LCD). The sensitivity test has been performed with detection limit up to 2.5 × 10(-3) ng/µL with different DNA concentrations of Salmonella bacteria. This system allows a rapid and automatic endpoint detection which could lead to the development of a point-of-care diagnosis device for foodborne pathogens detection in a resource-limited environment.

Matched MeSH terms: Salmonella/isolation & purification*

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