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  1. ras gene mutations in Malaysian leukemia patients
    Chin IY, Koh CL, Bosco JJ
    Acta Haematol., 1992;87(1-2):107-8.
    PMID: 1585764
    Matched MeSH terms: Genes, ras/genetics*
  2. Stability and expression of K-ras mimotopes in freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder during storage in vacuum packaging
    How YH, Teo MYM, In LLA, Yeo SK, Bhandari B, Yusof YA, et al.
    J Appl Microbiol, 2024 Jul 02;135(7).
    PMID: 38955370 DOI: 10.1093/jambio/lxae162
    AIMS: This study aims to evaluate the storage stability of the freeze-dried recombinant Lactococcus lactis NZ3900-fermented milk powder expressing K-ras (Kristen rat sarcoma viral oncogene homolog) mimotopes targeting colorectal cancer in vacuum packaging.

    METHODS AND RESULTS: The freeze-dried L. lactis-fermented milk powder stored in 4-ply retortable polypropylene (RCPP)-polyamide (PA)-aluminium (AL)-polyethylene terephthalate (PET) and aluminium polyethylene (ALPE) was evaluated throughout 49 days of accelerated storage (38°C and 90% relative humidity). The fermented milk powder stored in 4-ply packaging remained above 6 log10 CFU g-1 viability, displayed lower moisture content (6.1%), higher flowability (43° angle of repose), water solubility (62%), and survivability of L. lactis after simulated gastric and intestinal digestion (>82%) than ALPE packaging after 42 days of accelerated storage. K-ras mimotope expression was detected intracellularly and extracellularly in the freeze-dried L. lactis-fermented milk powder upon storage.

    CONCLUSIONS: This suggests that fermented milk powder is a suitable food carrier for this live oral vaccine.

    Matched MeSH terms: Genes, ras/genetics
  3. Fulltext Analysis of ras gene mutations in acute myeloid leukemia by the polymerase chain reaction and oligonucleotide probes
    Chin YM, Bosco JJ, Koh CL
    Singapore Med J, 1992 Feb;33(1):48-50.
    PMID: 1598607
    In vitro deoxyribonucleic acid (DNA) amplification by the polymerase chain reaction (PCR) followed by hybridization with oligonucleotide probes were used to study ras gene mutations in acute myeloid leukemia (AML). The DNA of 30 AML patients at presentation of the disease at the University of Malaya Hospital, Kuala Lumpur were screened for ras gene mutations in codons 12, 13 and 61 of the N-ras, K-ras and H-ras genes. Four patients (13.3%) had ras gene mutations. They were all below their early thirties in age. Of the four patients with ras gene mutations, three were M3 and one was M4 according to the French American British (FAB) classification of AML.
    Matched MeSH terms: Genes, ras/genetics*
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