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Comparative evaluation of the effectiveness of desensitizing agents in dentine tubule occlusion using scanning electron microscopy

Chen CL, Parolia A, Pau A, Celerino de Moraes Porto IC

Aust Dent J, 2015 Mar;60(1):65-72.

PMID: 25721280 DOI: 10.1111/adj.12275

Dentine hypersensitivity (DH) occurs on exposed dentine and is dependent on the patency of dentinal tubules. This study compared the effectiveness of red propolis extract (RPE), calcium sodium phosphosilicate (Novamin) and arginine-calcium carbonate (ACC) in occluding dentine tubules.

Matched MeSH terms: Dentin/ultrastructure

A comparative evaluation of cleaning efficacy (debris and smear layer removal) of hand and two NiTi rotary instrumentation systems (K3 and ProTaper): a SEM study

Reddy KB, Dash S, Kallepalli S, Vallikanthan S, Chakrapani N, Kalepu V

J Contemp Dent Pract, 2013 Nov 1;14(6):1028-35.

PMID: 24858745

The present study was conducted to compare the cleaning efficacy (debris and smear layer removal) of hand and two NiTi rotary instrumentation systems (K3 and ProTaper).

Matched MeSH terms: Dentin/ultrastructure

Racial variations in the extent of tooth root translucency in ageing individuals

Whittaker DK, Bakri MM

Arch Oral Biol, 1996 Jan;41(1):15-9.

PMID: 8833585

One hundred and ninety-eight single-rooted teeth from individuals aged 21-90 yr of Caucasian, Malay, Chinese Malay and Indian Malay origin were studied. Single or serial longitudinal sections of extracted teeth were cut following dye imbibition of patent dentinal tubules. The extent of sclerosis of apical dentinal tubules was assessed and correlated with the age of the individual. Correlation with age was higher in the Caucasian than the Malay races and within the Malaysian racial groups correlation with age was highest in the Malays and lowest in the Chinese. It is concluded that factors other than age may be important in the formation of sclerotic apical dentine in teeth of different racial origin. The effect of racial origin should be considered when using sclerosis as a means of age determination in forensic cases.

Matched MeSH terms: Dentin/ultrastructure*

The influence of experimental silane primers on dentin bond strength and morphology: a laboratory and finite element analysis study

Mustafa AA, Matinlinna JP, Saidin S, Kadir MR

J Prosthet Dent, 2014 Dec;112(6):1498-506.

PMID: 24993375 DOI: 10.1016/j.prosdent.2014.05.011

STATEMENT OF PROBLEM: The inconsistency of dentin bonding affects retention and microleakage.

PURPOSE: The purpose of this laboratory and finite element analysis study was to investigate the effects on the formation of a hybrid layer of an experimental silane coupling agent containing primer solutions composed of different percentages of hydroxyethyl methacrylate.

MATERIAL AND METHODS: A total of 125 sound human premolars were restored in vitro. Simple class I cavities were formed on each tooth, followed by the application of different compositions of experimental silane primers (0%, 5%, 25%, and 50% of hydroxyethyl methacrylate), bonding agents, and dental composite resins. Bond strength tests and scanning electron microscopy analyses were performed. The laboratory experimental results were validated with finite element analysis to determine the pattern of stress distribution. Simulations were conducted by placing the restorative composite resin in a premolar tooth by imitating simple class I cavities. The laboratory and finite element analysis data were significantly different from each other, as determined by 1-way ANOVA. A post hoc analysis was conducted on the bond strength data to further clarify the effects of silane primers.

RESULTS: The strongest bond of hybrid layer (16.96 MPa) was found in the primer with 25% hydroxyethyl methacrylate, suggesting a barely visible hybrid layer barrier. The control specimens without the application of the primer and the primer specimens with no hydroxyethyl methacrylate exhibited the lowest strength values (8.30 MPa and 11.78 MPa) with intermittent and low visibility of the hybrid layer. These results were supported by finite element analysis that suggested an evenly distributed stress on the model with 25% hydroxyethyl methacrylate.

CONCLUSIONS: Different compositions of experimental silane primers affected the formation of the hybrid layer and its resulting bond strength.

Matched MeSH terms: Dentin/ultrastructure*

Sealing ability of various restorative materials as coronal barriers between endodontic appointments

Tapsir Z, Aly Ahmed HM, Luddin N, Husein A

J Contemp Dent Pract, 2013 Jan 1;14(1):47-50.

PMID: 23579892

To evaluate and compare the microleakage of various restorative materials used as coronal barriers between endodontic appointments.

Matched MeSH terms: Dentin/ultrastructure

Fulltext A quaternary ammonium silane antimicrobial triggers bacterial membrane and biofilm destruction

Daood U, Matinlinna JP, Pichika MR, Mak KK, Nagendrababu V, Fawzy AS

Sci Rep, 2020 07 03;10(1):10970.

PMID: 32620785 DOI: 10.1038/s41598-020-67616-z

To study the antimicrobial effects of quaternary ammonium silane (QAS) exposure on Streptococcus mutans and Lactobacillus acidophilus bacterial biofilms at different concentrations. Streptococcus mutans and Lactobacillus acidophilus biofilms were cultured on dentine disks, and incubated for bacterial adhesion for 3-days. Disks were treated with disinfectant (experimental QAS or control) and returned to culture for four days. Small-molecule drug discovery-suite was used to analyze QAS/Sortase-A active site. Cleavage of a synthetic fluorescent peptide substrate, was used to analyze inhibition of Sortase-A. Raman spectroscopy was performed and biofilms stained for confocal laser scanning microscopy (CLSM). Dentine disks that contained treated dual-species biofilms were examined using scanning electron microscopy (SEM). Analysis of DAPI within biofilms was performed using CLSM. Fatty acids in bacterial membranes were assessed with succinic-dehydrogenase assay along with time-kill assay. Sortase-A protein underwent conformational change due to QAS molecule during simulation, showing fluctuating alpha and beta strands. Spectroscopy revealed low carbohydrate intensities in 1% and 2% QAS. SEM images demonstrated absence of bacterial colonies after treatment. DAPI staining decreased with 1% QAS (p

Matched MeSH terms: Dentin/ultrastructure

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