Introduction: The interpretations of printed panoramic radiographs film frequently depend on personal judgements analysis by observers. The findings correlations between more than two observers must consider statistical tool which can determine that sometimes the observers will agree or disagree merely by chance. Intraclass Correlation Coefficient (ICC) generally measures the degree of similarity among individuals within a cluster. Kappa statistic is the most frequently applied statistical analysis for this reason. This study aims to determine the validity of utilising printed panoramic radiographs for assessing dental anomalies and inter-examiner reliability in detecting dental anomalies observed within the panoramic radiographs. Methods: Twenty samples of printed versions of orthopantomogram (OPG) were arbitrarily selected from the AMDI Dental Clinic. Radiographic analyses were conducted twice, Trial 1 and Trial 2, with two-week intervals in between. Ten (10) printed OPG films were used in each session. During both assessments, incidence of dental anomalies was determined. All twenty OPGs were examined and analysed by the principal investigator and two independent examiners according to the inclusion and exclusion criterias. The data analysis was carried out using SPSS version 23. Results: The results of ICC for Trials 1 and 2 were 0.961 and 0.984, respectively. Conclusion: The outcomes demonstrated that the printed OPG films can be used to detect dental anomalies and inter-examiner dependability was in very good assertion for both trials, hence printed OPG films that satisfy the inclusion and exclusion criterias can be utilised for assessing dental anomalies as shown by the reliable ICC values in this study.
Panoramic radiograph is a routine investigation tool in orthodontic diagnosis and treatment planning however the limitation of two-dimensional image in assessing the position of impacted canine and surrounding tissues had cause unnecessary treatment options and complications during treatment. This report highlights the differences in investi- gation outcome and orthodontic treatment planning of two similar cases of patients with impacted upper canine, in which one only had panoramic radiograph and another case supplemented with Cone Beam Computed Tomography (CBCT). The case supported only by panoramic radiograph had changes in treatment planning during canine remov- al surgery as prevention from oro-antral fistula. This event could have been prevented if CBCT was taken where the canine can be assessed for proximity towards maxillary sinus. It is recommended for CBCT in orthodontic assessment for canine impaction especially for cases where canine is positioned higher than root apex of adjacent teeth and when root resorption is suspected.
Oral cancer remains as a worldwide health issue. In Malaysia, oral neoplasm contributed about 10.6% death at the government hospitals. The significant consequences of delaying referral and poor prognosis of oral cancer was mainly due to the lack of knowledge among public. This study aims to determine the level of awareness and knowledge on oral cancer among students with non-medical background in Kepala Batas, Pulau Pinang, Malaysia. A cross sectional study using questionnaires were conducted among systematically randomly selected 275 non-medical background students aged 18 years old and above. The association of smoking habit with oral cancer awareness and knowledge was evaluated using chi-square test. Factors associated with the knowledge were determined at both univariable and multivariable levels using simple logistic and multiple logistic regression analyses respectively. Oral cancer awareness level was high among the students (85%) even though knowledge on early signs of oral cancer was low (19 years old has 1.96 time the odds compared to students aged ≤19 to have poor knowledge while Malay has 4.28 time the odds compared to Indian to have poor knowledge. Although smoking was observed as the most recognisable risk factor of oral cancer (82%), the smoking prevalence was relatively high (46.9%). The smokers in this study had low awareness and knowledge on oral cancer compared to the non-smoker. Hence, preventive measures adopting knowledge transfer should be proposed to enhance individual awareness and knowledge on oral cancer with Smoking cessation programme to help the smoker to quit smoking
This study aims to determine the effects of bioactive glass (BG) combined with Acmella oleracea (AO) extracts on dental pulp stem cells (DPSC) viability. DPSC were exposed to different combinations of BG-AO leave extract-conditioned medium. The BG 45S5 powder was synthesized using the sol-gel method. AO extract was prepared using ethanol extraction method. Gas Chromatography–Mass Spectrometry (GCMS) analysis of the AO ethanol extract was performed on a GCMS system consisting of an Agilent 6890 gas chromatograph coupled with an Agilent 5973 mass spectrometer. Sol-gel BG conditioned medium doped with AO extracts at various concentrations (25, 50, 100 and 250 μg/mL) with BG (1 mg/ mL) were prepared and exposed to DPSC. The DPSC was also treated using BG- and AO- only conditioned medium and non-treated cell as control. The DPSC cells’ responses were assessed using Alamar Blue (AB) assay. The results showed that GCMS analysis revealed the presence of amide, ester, terpenoid, fatty acid, alkene, terpene, carbohydrate, phenolic and alkane groups. Based on the AB assay, the BGAO- conditioned medium promoted DPSC viability. However, an increase in DPSC cell viability is clearly observed at Day 7 and 14 following exposure in BGAO-conditioned medium at the ratio of 1 mg/mL BG with 50 and 100 μg/mL of AO in comparison with AO alone. BGAO-conditioned medium at a dose of 25 μg/mL supported greater DPSC viability compared to other combination doses. The effect of combination of BG and AO towards DPSC at a certain dosage revealed continuous cell viability over the observation period and promoted cell growth that may be contributed by the combined effects of BG dissolution ions into the culture medium and also the presence of identified compound from the AO extracts namely phytol, linoleic acid, palmitic acid and 1, 4, 7,-Cycloundecatriene, 1, 5, 9, 9-tetramethyl, Z, Z, Z. Thus, it may have a significant potential to help in promoting dental and hard tissue regeneration
Introduction: Chrysanthemum rubellum (durian) flower (CR) is well-known for its usefulness in conventional and advanced medicine. Bioactive glass properties are geared towards hard tissue regeneration. Hence, this study aims to investigate the response of dental pulp stem cells (DPSC) when exposed to bioactive glass-chrysanthemum flower ex- tract-conditioned medium. Methods: Chrysanthemum rubellum (durian) yellow coloured petals freeze dried extracts (CRE) was prepared by separation and agitation in distilled water where the final powdery compound was being investigated and combined with melt-derived BG 45S5 powder to produce BGCRE-conditioned medium. The CRE extracts in various concentrations and BGCRE-conditioned medium were exposed to DPSC and the cells responses were assessed using AB and MTT assays. The CRE and BGCRE-conditioned media were also assessed using ICP-OES to check for ionic release profile from both medium. Results: The CRE-conditioned medium (7.81, 15.63, 31.25 and
62.5 μg/ml) showed a dose-dependent effect towards DPSC from Days 1 until 14. The BGCRE-conditioned medium containing BG powders (1 mg/ml) with the lower amount of CRE extracts (0.02 and 0.1 mg/ml) promoted DPSC via- bility and proliferation rate from Days 1 until 14 based on AB and MTT assays. The BGCRE-conditioned medium has potentially affected the DPSC viability and proliferation. Conclusion: The presence of CRE in BGCRE-conditioned medium enhanced the DPSC viability and proliferation possibly through the combined effect of CRE and BG. This BGCRE combination showed potential as natural medicament for dental tissue regeneration.