The embodied carbon of building materials and the energy consumed during construction have a significant impact on the environmental credentials of buildings. The structural systems of a building present opportunities to reduce environmental emissions and energy. In this regard, mass timber materials have considerable potential as sustainable materials over other alternatives such as steel and concrete. The aim of this investigation was to compare the environment impact, energy consumption, and life cycle cost (LCC) of different wood-based materials in identical single-story residential buildings. The materials compared are laminated veneer lumber (LVL) and glued laminated timber (GLT). GLT has less global warming potential (GWP), ozone layer depletion (OLD), and land use (LU), respectively, by 29%, 37%, and 35% than LVL. Conversely, LVL generally has lower terrestrial acidification potential (TAP), human toxicity potential (HTP), and fossil depletion potential (FDP), respectively, by 30%, 17%, and 27%. The comparative outcomes revealed that using LVL reduces embodied energy by 41%. To identify which of these materials is the best alternative, various environmental categories, embodied energy, and cost criteria require further analysis. Therefore, the multi-criteria decision-making (MCDM) method has been applied to enable robust decision-making. The outcome showed that LVL manufacturing using softwood presents the most sustainable choice. These research findings contribute to the body of knowledge about the use of mass timber in construction.