The skin can be vulnerable to various microbial infection. Although antibiotics are clinically proven to be useful in the treatment of bacterial skin infections, they are largely subjected to antibiotic resistance and adverse effects. This has led to the screening of several medicinal plants for their potential antimicrobial activity since they are less expensive, has reduced occurrence of adverse effects and widespread availability. The aim of this research will focus on evaluating the antibacterial activity of different extracts of Ipomoea aquatica leaves against Staphylococcus aureus and Streptococcus pyogenes that causes skin infections. Leaves were extracted separately with 95% methanol and 95% ethanol using maceration process. Phytochemical screening was done for each extract and the minimum inhibitory concentration (MIC) was determined for each extract against both bacteria using 10 different concentrations ranging from 10mg/ml up to 100mg/ml via disc diffusion method in triplicates. Two concentrations above the MIC from each extract were selected and antibacterial assay of the different extracts against the two bacteria respectively was performed using disc diffusion method in triplicates. MIC for methanolic extract against both bacteria was 10mg/ml, while MIC for ethanolic extract was 10mg/ml against Staphylococcus aureus and 30mg/ml against Streptococcus pyogenes. Methanolic extract of the plant at a concentration of 90mg/ml and 100mg/ml was statistically significant against Streptococcus pyogenes with a significance value of 0.00 (p
Bacterial infections have become a major cause of morbidity and mortality over the recent years and gastrointestinal infections are one of the common diseases caused Gram-negative bacteria such as Escherichia coli, Shigella sp. and Salmonella typhi. Treatment involves usage of wide spectrum antibiotics like fluoroquinolones often leads to development of antibiotic resistance and by implementing medicinal plants usage in treating diseases are believed to be effective against bacteria, the need for conventional therapy and risks of antibiotic resistance are reduced. Ipomoea aquatica (Family: Convolvulaceae) are commonly known as kangkung leaves. Various pharmacological activities such as anti- hyperglycaemic, anti- inflammatory and diuretics were explored except for its antibacterial activity. The aim of this study is to evaluate the antibacterial activity between methanolic and ethanolic extracts of Ipomoea aquatica leaves against Escherichia coli and Salmonella typhi. I. aquatica leaves were extracted with 95% methanol and 95% ethanol and their phytochemical properties were determined. MIC was determined for both methanolic and ethanolic extracts of Ipomoea aquatica against Escherichia coli and Salmonella typhi, using concentrations ranging from 10mg/ml up to 100mg/ml via disc diffusion method. Two concentrations were chosen from each extract to evaluate antibacterial activity using disc diffusion method with ciprofloxacin as positive control and distilled water as the negative control, done in triplicates. Data was entered on SPSS statistics version 23 and analysed using one way ANOVA and post hoc Tukey. Based on ANOVA, antibacterial activity was more profound in concentrations from methanolic extracts of Ipomoea aquatica leaves against Salmonella typhi, where the both concentrations at 40mg/ml and 100mg/ml showed significant antibacterial activity against Salmonella typhi when compared to the positive control Ciprofloxacin (5mg/disc), producing a significant value p< 0.05. Both methanolic and ethanolic extracts of Ipomoea aquatica leaves produced antibacterial activity against Escherichia coli and Salmonella typhi.