Exposure to organophosphate pesticide including fenitrothion (FNT) has led to many adverse effects on human health.
However, a potent antioxidant like palm oil tocotrienol-rich fraction (TRF) can reduce oxidative damage in various
pathological conditions, could also reduce the adverse effects by FNT. The aim of this study was to evaluate the effect
of TRF on oxidative liver damage in FNT induce hepatotoxicity in experimental rats. A total of 40 male Sprague-Dawley
rats were randomly divided into four groups of 10, namely control, TRF, FNT and TRF+FNT group. TRF (200 mg/kg
body weight) and FNT (20 m/kg body weight) were administered through oral gavage for 28 days. Corn oil which
served as vehicle was given orally to the control group. At the end of the study period, liver and blood was taken for
oxidative damage and biochemical evaluation and histological observation. TRF supplementation prevents oxidative
liver damage by reducing the hepatic malondialdehyde (MDA) and protein carbonyl (PCO) level significantly. Besides,
TRF also restored the endogenous antioxidants particularly reduced glutathione (GSH), glutathione peroxidase (GPx)
and ferric reducing/antioxidant power (FRAP). TRF also prevent liver damage by reducing the liver enzymes, alanine
aminotransferase (ALT) and aspartate aminotransferase (AST). The attenuation of liver damage by TRF was also showed
histologically. In conclusion, TRF supplementation showed a potential in preventing oxidative liver damage in FNTtreated
rats by reducing the oxidative damage and improving the antioxidant status.
Fenitrothion (FNT) usage has received much attention for its potential to promote free radicals generation and interfere with antioxidant defense system. The aim of the present study was to investigate the effect of palm oil tocotrienol rich fraction (TRF) supplementation on oxidative stress and histological changes in rat brain induced by FNT. A total of 32 male Sprague Dawley rats divided into four groups: control group which received corn oil; TRF group was received palm oil TRF (200 mg/kg bw); FNT group administered with FNT (20 mg/kg bw) and TRF+FNT group pretreated with palm oil TRF (200 mg/kg bw) 30 min prior to administration of FNT (20 mg/kg bw). FNT and TRF were dissolved in corn oil and all supplementations were given by oral gavage once daily for 28 days. After four weeks of supplementation, TRF+FNT rats had significantly lower malondialdehyde (MDA) content and superoxide dismutase (SOD) activity but higher reduced glutathione (GSH) level and total protein level compared to FNT rats (p<0.05). However, protein carbonyl (PC) level was insignificantly lower for TRF+FNT group compared to FNT group. In conclusion, this study suggested that palm oil TRF was effective in preventing brain damage in rats.