Displaying publications 1 - 20 of 35 in total

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  1. Fulltext Assessment of patient satisfaction with the preoperative anaesthetic evaluation
    Lee, S.Y., Cheah, S.K., Muhammad, M., Aniza, I.
    Medicine & Health, 2020;15(1):156-165.
    MyJurnal
    Penilaian anestetik pra-operasi adalah penting semasa penjagaan perioperatif. Matlamat utama kajian ini adalah untuk menilai tahap kepuasaan pesakit dan faktor-faktor yang mempengaruhi kepuasaan pesakit terhadap klinik pra-anestetik. Sejumlah 304 orang pesakit yang menghadiri klinik pra-anestetik telah terlibat dalam kajian ini. Kajian kepuasaan selidik dwibahasa (Bahasa Inggeris dan Bahasa Melayu) dengan berjumlah 18 soalan menguji faktor-faktor bukan penyedia (kemudahan klinik, kesesuaian masa menunggu) dan faktor-faktor penyedia (perkhidmatan doktor, pegawai kaunter, jururawat dan kakitangan sokongan) telah dikaji. Kajian kepuasaan selidik menggunakan format skala Likert lima mata skor sangat setuju/tidak setuju telah digunakan untuk menilai tahap kepuasaan pesakit. Secara keseluruhan, peratusan kepuasaan pesakit terhadap penilaian pra-anestetik dilaporkan sebagai 98.7% dengan skor purata untuk perkhidmatan doktor (4.49 + 0.60), pegawai kaunter (4.48 + 0.61), kakitangan sokongan (4.47 + 0.62), kemudahan klinik dan kesesuaian masa menunggu (4.40 + 0.62). Semua faktor-faktor yang dikaji menunjukkan hubungan yang signifikan (p
  2. Chemical synthesis of metal nanoparticles in aqueous solutions with the presence of some additives
    Oyama M, Akrajasali Umar, Muhammad M Atsalleh, Burhanuddin Eopmajlis
    Sains Malaysiana, 2011;40:1345-1353.
    Metal nanoparticles having interesting shapes can be prepared in aqueous solutions through simple reductions of metal ions with the presence of some additive reagents, such as cetyltrimethylammonium bromide and hexamethylenetetramine. In this review, some successful results for shape-controlled synthesis of metal nanoparticles in our group are summarized, which includes the synthesis of palladium nanocubes, palladium nanobricks, gold nanotripods. In addition, combining with indium tin oxide electrode surfaces, shape-controlled growth is shown to be possible to form gold nanoplates and copper oxide nanowires. Even in relatively mild synthetic conditions, interesting shape-controlled synthesis of metal nanoparticles is possible.
  3. Fulltext A crossover study comparing in-plane and out-of-plane approaches for simulated ultrasound- guided central venous cannulation on phantom models by anaesthesiology trainees
    Yap, K.B., Shereen, T.S.P., Yeoh, C.N., Liu, C.Y., Nadia, M.N., Joanna, O.S.M., et al.
    Medicine & Health, 2019;14(1):135-144.
    MyJurnal
    This prospective crossover study compared the incidence of posterior vessel wall puncture between two approaches during ultrasound-guided simulated central venous cannulation by anaesthesiology trainees. Each phantom model, simulating a central vein and artery, was cannulated by 37 anaesthesiology trainees under ultrasound-guidance using the in-plane approach (IPA) and out-of-plane approach (OPA). Total procedural time and the time taken from starting image scanning until commencing puncture, was recorded. The number of attempts required to achieve successful venous cannulation was noted. Finally, the models were examined for posterior venous wall and arterial puncture. Total procedural time was shorter with the OPA (26.5 vs 50.3 seconds, p=0.001). The time taken from starting image scanning until commencing puncture was shorter for the OPA (2.2 vs 12.3 seconds, p
  4. Fulltext Post-intubation airway related adverse effects: a aomparison between intra-cuff dexamethasone and intra-cuff alkalinized lignocaine
    Kep, Kee W., Nadia, M.N., Melvin, K., Muhammad, M., Raha, R., Nurlia, Y.
    Journal of Surgical Academia, 2013;3(2):26-31.
    MyJurnal
    Post-intubation airway related adverse effects such as coughing on the endotracheal tube (ETT), restlessness, hoarseness and sore throat are common and undesirable outcomes of anaesthesia using endotracheal intubation. This prospective randomized single blind study was carried out to compare the effectiveness of intra-cuff dexamethasone and alkalinized lignocaine in reducing the incidence of post-intubation airway related adverse effects. Eighty four patients aged 18 – 60 years, of ASA status I or II, were randomly allocated into three groups: air, dexamethasone and alkalinized lignocaine. Their ETT cuffs were inflated according to the group they were allocated to. The incidence of coughing on the ETT, restlessness, hoarseness and sore throat was assessed, postoperatively. The results showed a significant difference in the incidence of cough, restlessness, hoarseness and sore throat in the dexamethasone group compared to the air group. All the patients had minimal or no sore throat at all documented times. Both intra-cuff dexamethasone and alkalinized lignocaine significantly reduced the incidence of hoarseness. However, alkalinized lignocaine additionally lowered the incidence of restlessness, significantly.
  5. Fulltext Comparison between patient-controlled epidural analgesia and continuous epidural infusion for pain relief after gynaecological surgery
    Suhaila, N., Nurlia, Y., Azmil Farid, Z., Melvin, K., Muhammad, M., Nadia, M.N.
    Journal of Surgical Academia, 2013;3(2):14-19.
    MyJurnal
    This prospective, randomised study compared the effectiveness of patient controlled epidural analgesia (PCEA) versus continuous epidural infusion (CEI) in providing pain relief post gynaecological surgery. Sixty six ASA I or II patients planned for gynaecological surgery via Pfannensteil incision under combined spinal epidural anaesthesia were recruited. They were randomised into two groups: Group A patients received PCEA while Group B patients received CEI. In the recovery area, both groups received an epidural combination of levobupivacaine 0.1% and fentanyl 2 μg/ml. Group A patients were allowed demand bolus doses of 5 ml with a 20 minute lockout interval, while Group B patients had their epidural infusion initiated at 6 ml/hour with increments as required to a maximum of 12 ml/hour. Pain score and degree of motor blockade was assessed hourly in the first four hours and subsequently at four hourly intervals. Side effects were recorded at four-hourly interval. The total amount of analgesia, number of anaesthetic interventions and patient satisfaction was assessed 24 hours, postoperatively. There was no significant difference in pain score, total amount of analgesia, number of anaesthetic interventions and patient satisfaction. The degree of motor blockade and side effects were comparable between the groups. In conclusion, PCEA was comparable to CEI for pain relief after gynaecological surgery.
  6. Sedation with dexmedetomidine versus propofol during regional anaesthesia: comparing haemodynamic parameters, respiratory rates and offset times
    Nadia, M.N., Samsul Johari, M.A., Muhammad, M., Raha, A.R., Nurlia, Y.
    Journal of Surgical Academia, 2012;2(2):15-20.
    MyJurnal
    This study aimed to compare dexmedetomidine and propofol, in terms of haemodynamic parameters, respiratory rates and offset times, when used for sedation in patients undergoing elective orthopaedic and surgical procedures under regional anaesthesia. This was a prospective, randomised, single-blind study where 88 patients were recruited. Patients were randomised into two groups to receive either dexmedetomidine or propofol infusion. Central neuraxial blockade (spinal, epidural or combined spinal epidural) was performed. After ensuring an adequate block and stable haemodynamic parameters, dexmedetomidine was infused 15 minutes later at 0.4 μg/kg/hr, and propofol, at a target concentration of 2.5 μg/ml. Both drugs were titrated to achieve a bispectral index score of 70 before surgery commenced. Sedation level was monitored using the bispectral index score and assessed by the Observer Assessment of Alertness Scale score. Drug infusion was adjusted to maintain bispectral index scores ranging between 70-80 during surgery. Both groups showed reductions in mean arterial pressure and heart rate from baseline readings throughout the infusion time. However there was no significant reduction in the first 15 minutes from baseline (p > 0.05). Haemodynamic parameters and respiratory rate between both groups were not significantly different (p > 0.05). No patient demonstrated significant respiratory depression or SpO2 ≤ 95%. Offset times were also not significantly different between both groups (p = 0.594). There were no significant differences in haemodynamic parameters, respiratory rates and offset times between dexmedetomidine and propofol used for sedation in patients undergoing elective orthopaedic and surgical procedures under regional anaesthesia.
  7. Fulltext Comparison between magnesium sulphate and esmolol in attenuating haemodynamic responses to laryngoscopy and endotracheal intubation
    Norhuzaimah, J., Liu, C. Y., Muhammad, M., Joanna Ooi ,S. M.
    Journal of Surgical Academia, 2018;8(1):0-0.
    MyJurnal
    During induction of general anaesthesia, the act of laryngoscopy and tracheal intubation stimulates the sympathetic
    nervous system resulting in an increase in blood pressure and heart rate which may be harmful especially in elderly
    patients with pre-existing ischaemic heart disease. Several drugs have therefore been used to obtund this increase
    including esmolol, nicardipine, magnesium sulphate and lignocaine. This prospective, double blind randomised
    clinical trial compared the efficacy of magnesium sulphate and esmolol in attenuating haemodynamic responses to
    laryngoscopy and tracheal intubation. One hundred and twenty six ASA I-II patients scheduled for elective surgery
    requiring general anaesthesia with tracheal intubation were enrolled and randomised into two groups: Group 1 (n =
    67) received MgSO4 40 mg/kg diluted in 100 ml normal saline administered over ten minutes, whereas Group 2 (n =
    59) received a bolus of esmolol 1.0 mg/kg diluted to 10 ml. Systolic and diastolic blood pressures and heart rate were
    recorded every minute for subsequent 10 minutes following laryngoscopy and tracheal intubation. Attenuation of the
    mean systolic and diastolic blood pressures following laryngoscopy and tracheal intubation was significantly larger
    in Group 2 compared to Group 1. Patients in Group 2 had significantly better suppression of heart rate response
    compared to Group 1 during the first four minutes after laryngoscopy and tracheal intubation (p
  8. Self-assembled reduced graphene oxide nanoflakes assisted by post-sonication boosted electrical performance in gold interdigitated microelectrodes
    Taniselass S, Arshad MKM, Gopinath SCB, Ramli MM
    J Colloid Interface Sci, 2020 Oct 01;577:345-354.
    PMID: 32485416 DOI: 10.1016/j.jcis.2020.05.070
    Reduced graphene oxide (rGO) is widely utilised to develop various types of biosensors; however, producing self-assembled rGO nanoflake networks through single-droplet drop-casting remains inconsistent. In the present work, we systematically used three different methods to prepare rGO suspensions in order to produce large scale self-assembled rGO nanoflake networks through single-droplet drop-casting. The rGO suspensions were prepared using only deionised water with no added any chemicals/organic solvents, which we considered to be a low-cost method. Subsequently, the most effective preparation method was used to deposit rGO nanoflakes onto commercial gold interdigitated microelectrodes (Au-IDE) to examine their electrical performance. Assessment of the yields, developed methods, surface morphologies, spectroscopy and structural analyses of the as-prepared rGO nanoflakes were conducted. The results revealed that method-3 (involving sonication, centrifugation and post-sonication) produced large self-assembled rGO nanoflake networks with strong adhesion to glass substrates. Furthermore, the as-prepared rGO/Au-IDE modified sensors showed excellent electron mobility where the electrical conductivity was enhanced approximately ~ 1000 fold compared to the bare devices. The present work provided new insights for depositing large self-assembled interconnected rGO nanoflake networks through single-droplet drop-casting which will be beneficial for biosensor development and other downstream applications.
  9. Application of DEA-Based Malmquist Productivity Index on Health Care System Efficiency of ASEAN Countries
    Singh S, Bala MM, Kumar N, Janor H
    Int J Health Plann Manage, 2021 Jul;36(4):1236-1250.
    PMID: 33855765 DOI: 10.1002/hpm.3169
    This study assesses and compares the productive efficiency of the national healthcare system of the ASEAN region which includes Brunei, Cambodia, Indonesia, Laos, Malaysia, Myanmar, Philippines, Singapore, Thailand and Vietnam amidst rising mortality rate from noncommunicable diseases (NCDs) in the Sustainable Development Goals (SDGs) era. Nonparametric data envelopment analysis technique based on the Malmquist Productivity Index is performed and its components, total factor productivity change, technical change and technological change are compared across the region. Two different models are considered in assessing and comparing the technical efficiency of the national healthcare system across the region with life expectancy at birth and mortality rate from NCDs as parallel health care output for both the models. The mean value of total factor productivity is 0.983 and 0.974 which suggests that national healthcare system productivity efficiency decays by 1.7% for Model I and 2.6% for Model II, respectively. This suggests that the health care system inefficiencies across the ASEAN region have not made life expectancy to improve as much as it should be and curtailed the mortality rate from growing chronic NCDs within a decade. The region is likely to lag behind in achieving SDGs 3 target 4 on reducing by one-third premature mortality from chronic NCDs unless the health care system's technical efficiency is improved across the region. The finding suggests a microlevel study on each country to identify major sources of healthcare system inefficiency in a bid to ameliorate it.
  10. Fulltext Effectiveness of cataract surgery with intraocular lens implantation: a comparative study between extracapsular cataract extraction and phacoemulsification
    Rizal, A.M., Muhammad, M., Aljunid, S.M., Normalina, M., Faridah Hanom, A., Chuah, K.L., et al.
    Malaysian Journal of Community Health, 2007;13(1):32-37.
    MyJurnal
    Background: A randomised single blinded clinical trial comparing the effectiveness of two methods of cataract surgery with intraocular lens implantation: extracapsular cataract extraction (ECCE) and phacoemulsification (PEA) was carried out at Hospital Universiti Kebangsaan Malaysia (HUKM) between March 2000 and August 2001. Methods: The effectiveness of cataract surgery was assessed from the quality of life specifically for vision via Visual Function 14 (VF-14) preoperatively, one week, two months and six months after surgery. Results: The result showed that there was a significant increased in VF-14 scores after a week, two months and six months postoperation compared to the score before surgery for both techniques. However there was no significant difference in VF-14 scores when compared between ECCE and PEA. Conclusions: This study indicated that both techniques give equal benefit to cataract patients. Since effectiveness of cataract surgery with intraocular lens implantation is unrelated to operative procedures, less costly technique should be promoted.
  11. Fulltext Drug reaction with herbal supplement: a possible case of drug induced lupus erythematosus
    Muthupalaniappen L, Jamani N, Muhammad M, Adawiyah J, Aziz N
    Malays Fam Physician, 2010;5(2):99-100.
    PMID: 25606196 MyJurnal
    A 24-year-old lady presented with four days history of fever, non-pruritic rash, ankle pain and swelling. She had consumed herbal supplement five days before the onset of symptoms. Examinations revealed erythematous maculo-papular lesions of varying sizes on sun exposed areas. Patient was suspected to have Drug Induced Lupus Erythematosus (DILE) and subsequently symptoms subsided rapidly on withholding the herbal medication.
  12. THE LEGALITY OF EUTHANASIA FROM THE MALAYSIAN AND ISLAMIC PERSPECTIVES: AN OVERVIEW
    Alias F, Muhammad M, Kassim PNJ
    Med Law, 2015 Sep;34(1):509-532.
    PMID: 30759949
    The subject of euthanasia has generated many controversial debates, particularly on its legality. This has been primarily due to the doctrine of sanctity of life which is a predominant principle in many religions and is embedded in moral values. The underlying precept is that human life is sacred and demands respect, as all life comes from God and life can only be taken away intentionally through due process of law. Thus, ending a person's life, even upon his or her request, is considered in many jurisdictions as a criminal offence. Irrespective of humanitarian motives, a doctor who aids a patient in this act will be in breach of his unequivocal duty as a doctor and considered to have committed an unlawful act, which will subject him to legal sanctions. Countries such as the United Kingdom, Australia, New Zealand and Turkey have explicit legal provisions banning, in particular, active euthanasia, while countries such as the Netherlands, Belgium, Switzerland and certain states in the U.S have taken positive steps to legalise euthanasia in certain aspects. The ethical codes and laws in Malaysia have yet to develop to the fullest extent in dealing with these issues. Although Malaysian statutory legislation contains provisions banning active euthanasia, the legal position on passive euthanasia remains implicit. In the absence of indicative legal provisions and judicial precedents governing certain matters arising, it has been the practice of the Malaysian courts to refer to relevant legal principles inherent in English cases. As Islam is proclaimed as the country's official religion, the Islamic perspective is also an important and influential factor in the development of the legal framework in Malaysia as a whole. Thus, there is a need for the existence of a clear and comprehensive regulatory framework governing the legality of euthanasia in Malaysia.
  13. Fulltext Torsional Crack Localization in Palm Oil Clinker Concrete Using Acoustic Emission Method
    Khan S, Yap SP, Tan CG, Ganasan R, Sherif MM, El-Shafie A
    Materials (Basel), 2021 Sep 20;14(18).
    PMID: 34576670 DOI: 10.3390/ma14185446
    Palm oil clinker (POC) aggregates is a viable alternative to the naturally occurring sand and gravel in the manufacturing of concrete. The usage of POC aggregates assists in the reduction of solid waste and preserves the consumption of natural resources. Although researchers investigated the mechanical response of POC-containing concrete, limited research is available for its torsional behavior. In general, the torsional strength depends on the tensile strength of concrete. This research investigates the compressive, tensile, and torsional response of concrete with various ratios of POC-aggregates. Five batches of concrete were casted with POC-aggregate replacing granite at ratios of 0, 20, 40, 60, and 100%. The selection for the mixture proportions for the various batches was based on the design of experiments (DOE) methodology. The hard density, compressive strength, splitting tensile strength, and flexural strength of concrete with a 100% replacement of granite with POC-aggregates reduced by 8.80, 37.25, 30.94, and 14.31%, respectively. Furthermore, a reduction in initial and ultimate torque was observed. While cracks increased with the increase in POC-aggregates. Finally, the cracking of concrete subjected to torsional loads was monitored and characterized by acoustic emissions (AE). The results illustrate a sudden rise in AE activities during the initiation of cracks and as the ultimate cracks were developed. This was accompanied by a sudden drop in the torque/twist curve.
  14. Fulltext A science framework (SF) for agricultural sustainability
    Ahmed F, Al-Amin AQ, Masud MM, Kari F, Mohamad Z
    An Acad Bras Cienc, 2015 Sep;87(3):1887-902.
    PMID: 26221988 DOI: 10.1590/0001-3765201520130368
    The significance of Science Framework (SF) to date is receiving more acceptances all over the world to address agricultural sustainability. The professional views, however, advocate that the SF known as Mega Science Framework (MSF) in the transitional economies is not converging effectively in many ways for the agricultural sustainability. Specially, MSF in transitional economies is mostly incapable to identify barriers in agricultural research, inadequate to frame policy gaps with the goal of strategizing the desired sustainability in agricultural technology and innovation, inconsistent in finding to identify the inequities, and incompleteness to rebuild decisions. Therefore, this study critically evaluates the components of MSF in transitional economies and appraises the significance, dispute and illegitimate issue to achieve successful sustainable development. A sound and an effective MSF can be developed when there is an inter-linkage within principal components such as of (a) national priorities, (b) specific research on agricultural sustainability, (c) adequate agricultural research and innovation, and (d) alternative policy alteration. This maiden piece of research which is first its kind has been conducted in order to outline the policy direction to have an effective science framework for agricultural sustainability.
  15. Challenges in volunteering from cancer care volunteers perspectives
    Kamaludin KM, Muhammad M, Wahat NW, Ibrahim R
    Asian Pac J Cancer Prev, 2013;14(8):4795-800.
    PMID: 24083746
    The involvement of non-government organizations (NGOs) and support groups has helped strengthen public health services in addressing cancer care burden. Owing to the contribution of volunteers in cancer care, this article documents a qualitative study that examined challenges in attracting and retaining cancer care volunteers as part of the effort to develop a volunteer recruitment model. Data were collected through three focus group discussions involving 19 cancer support group members in Malaysia. Findings of the study revealed that mobility and locality appeared to be significant in Malaysian context, while the need for financial support and time flexibility are challenges faced by cancer support groups to attract and retain volunteers. The findings imply that cancer care initiatives can benefit from more local volunteers but at the same time these volunteers require flexibility and financial support to sustain their engagement.
  16. Fulltext A comparison between Laryngeal Tube Suction II Airway and Proseal Laryngeal Mask Airway in laparascopic surgery
    Esa K, Azarinah I, Muhammad M, Helmi MA, Jaafar MZ
    Med J Malaysia, 2011 Aug;66(3):182-6.
    PMID: 22111436
    This was a prospective randomized study comparing the ease of insertion, haemodynamic changes, quality of airway seal, oxygenation and ventilation parameters and complications between Laryngeal Tube Suction II (LTS II) with Proseal Laryngeal Mask Airway (PLMA), both are supraglottic airway incorporated with gastric passage. Fifty-four ASA I and II patients were randomly allocated to receive either LTS II or PLMA. Both devices provided a secure airway even under conditions of elevated intra-abdominal pressure up to 17 mmHg. In this study, there were no differences concerning ease of insertion, haemodynamic changes, quality of airway seal, oxygenation and ventilation parameters and complications between LTS II and PLMA.
  17. Impact of Article Page Count and Number of Authors on Citations in Disability Related Fields: A Systematic Review Article
    Ahmed A, Adam M, Ghafar NA, Muhammad M, Ebrahim NA
    Iran J Public Health, 2016 Sep;45(9):1118-1125.
    PMID: 27957456
    BACKGROUND: Citation metrics and total publications in a field has become the gold standard for rating researchers and viability of a field. Hence, stimulating demand for citation has led to a search for useful strategies to improve performance metric index. Meanwhile, title, abstract and morphologic qualities of the articles attract researchers to scientific publications. Yet, there is relatively little understanding of the citation trend in disability related fields. We aimed to provide an insight into the factors associated with citation increase in this field. Additionally, we tried to know at what page number an article might appear attractive to disability researchers needs. Thus, our focus is placed on the article page count and the number of authors contributing to the fields per article.

    METHODS: To this end, we evaluated the quantitative characteristics of top cited articles in the fields with a total citation (≥50) in the Web of Science (WoS) database. Using one-way independent ANOVA, data extracted spanning a period of 1980-2015 were analyzed, while the non-parametric data analysis uses Kruskal-Walis test.

    RESULTS: Articles with 11 to 20 pages attract more citations followed by those within the range of zero to 10. Articles with upward 21 pages are the least cited. Surprisingly, articles with more than two authors are significantly (P<0.05) less cited and the citation decreases as the number of authors increased.

    CONCLUSION: Collaborative studies enjoy wider utilization and more citation, yet discounted merit of additional pages and limited collaborative research in disability field is revealed in this study.

  18. Introgression of blast resistance genes into the elite rice variety MR263 through marker-assisted backcrossing
    Hasan MM, Rafii MY, Ismail MR, Mahmood M, Alam MA, Abdul Rahim H, et al.
    J Sci Food Agric, 2016 Mar 15;96(4):1297-305.
    PMID: 25892666 DOI: 10.1002/jsfa.7222
    Blast caused by the fungus Magnaporthe oryzae is a significant disease threat to rice across the world and is especially prevalent in Malaysia. An elite, early-maturing, high-yielding Malaysian rice variety, MR263, is susceptible to blast and was used as the recurrent parent in this study. To improve MR263 disease resistance, the Pongsu Seribu 1 rice variety was used as donor of the blast resistance Pi-7(t), Pi-d(t)1 and Pir2-3(t) genes and qLN2 quantitative trait locus (QTL). The objective was to introgress these blast resistance genes into the background of MR263 using marker-assisted backcrossing with both foreground and background selection.
  19. Fulltext Cell viability and electrical response of breast cancer cell treated in aqueous graphene oxide solution deposition on interdigitated electrode
    Ramli MM, Rosman AS, Mazlan NS, Ahmad MF, Halin DSC, Mohamed R, et al.
    Sci Rep, 2021 10 19;11(1):20702.
    PMID: 34667216 DOI: 10.1038/s41598-021-00171-3
    Breast cancer is one of the most reported cancers that can lead to death. Despite the advances in diagnosis and treatment procedures, the possibility of cancer recurrences is still high in many cases. With that in consideration, researchers from all over the world are showing interest in the unique features of Graphene oxide (GO), such as its excellent and versatile physicochemical properties, to explore further its potential and benefits towards breast cancer cell treatment. In this study, the cell viability and electrical response of GO, in terms of resistivity and impedance towards the breast cancer cells (MCF7) and normal breast cells (MCF10a), were investigated by varying the pH and concentration of GO. Firstly, the numbers of MCF7 and MCF10a were measured after being treated with GO for 24 and 48 h. Next, the electrical responses of these cells were evaluated by using interdigitated gold electrodes (IDEs) that are connected to an LCR meter. Based on the results obtained, as the pH of GO increased from pH 5 to pH 7, the number of viable MCF7 cells decreased while the number of viable MCF10a slightly increased after the incubation period of 48 h. Similarly, the MCF7 also experienced higher cytotoxicity effects when treated with GO concentrations of more than 25 µg/mL. The findings from the electrical characterization of the cells observed that the number of viable cells has corresponded to the impedance of the cells. The electrical impedance of MCF7 decreased as the number of highly insulating viable cell membranes decreased. But in contrast, the electrical impedance of MCF10a increased as the number of highly insulating viable cell membranes increased. Hence, it can be deduced that the GO with higher pH and concentration influence the MCF7 cancer cell line and MCF10a normal breast cell.
  20. Fulltext A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh
    Hooper C, Debnath PP, Biswas S, van Aerle R, Bateman KS, Basak SK, et al.
    Viruses, 2020 10 02;12(10).
    PMID: 33023199 DOI: 10.3390/v12101120
    Mass mortalities of the larval stage of the giant freshwater prawn, Macrobrachium rosenbergii, have been occurring in Bangladesh since 2011. Mortalities can reach 100% and have resulted in an 80% decline in the number of hatcheries actively producing M. rosenbergii. To investigate a causative agent for the mortalities, a disease challenge was carried out using infected material from a hatchery experiencing mortalities. Moribund larvae from the challenge were prepared for metatranscriptomic sequencing. De novo virus assembly revealed a 29 kb single‑stranded positive-sense RNA virus with similarities in key protein motif sequences to yellow head virus (YHV), an RNA virus that causes mass mortalities in marine shrimp aquaculture, and other viruses in the Nidovirales order. Primers were designed against the novel virus and used to screen cDNA from larvae sampled from hatcheries in the South of Bangladesh from two consecutive years. Larvae from all hatcheries screened from both years were positive by PCR for the novel virus, including larvae from a hatchery that at the point of sampling appeared healthy, but later experienced mortalities. These screens suggest that the virus is widespread in M. rosenbergii hatchery culture in southern Bangladesh, and that early detection of the virus can be achieved by PCR. The hypothesised protein motifs of Macrobrachium rosenbergii golda virus (MrGV) suggest that it is likely to be a new species within the Nidovirales order. Biosecurity measures should be taken in order to mitigate global spread through the movement of post-larvae within and between countries, which has previously been linked to other virus outbreaks in crustacean aquaculture.
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