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Female sterilization using Filshie clip in private practice

Puraviappan, Hamid Arshat, Jaffar Ali

Malays J Reprod Health, 1983 Jan;1(1):40-5.
PMID: 12279888
The effect of oral contraceptives in Malaysians: 1. iron metabolism and erythropoiesis

Jaffar Ali, Hamid Arshat, Khalid Hassan, Noor Laily Abu Bakar

Malays J Reprod Health, 1983 Jan;1(1):60-8.
PMID: 12279891
Next-generation viral nanoparticles for targeted delivery of therapeutics: Fundamentals, methods, biomedical applications, and challenges

Tan JS, Jaffar Ali MNB, Gan BK, Tan WS

Expert Opin Drug Deliv, 2023;20(7):955-978.
PMID: 37339432 DOI: 10.1080/17425247.2023.2228202

INTRODUCTION: Viral nanoparticles (VNPs) are virus-based nanocarriers that have been studied extensively and intensively for biomedical applications. However, their clinical translation is relatively low compared to the predominating lipid-based nanoparticles. Therefore, this article describes the fundamentals, challenges, and solutions of the VNP-based platform, which will leverage the development of next-generation VNPs.
AREAS COVERED: Different types of VNPs and their biomedical applications are reviewed comprehensively. Strategies and approaches for cargo loading and targeted delivery of VNPs are examined thoroughly. The latest developments in controlled release of cargoes from VNPs and their mechanisms are highlighted too. The challenges faced by VNPs in biomedical applications are identified, and solutions are provided to overcome them.
EXPERT OPINION: In the development of next-generation VNPs for gene therapy, bioimaging and therapeutic deliveries, focus must be given to reduce their immunogenicity, and increase their stability in the circulatory system. Modular virus-like particles (VLPs) which are produced separately from their cargoes or ligands before all the components are coupled can speed up clinical trials and commercialization. In addition, removal of contaminants from VNPs, cargo delivery across the blood brain barrier (BBB), and targeting of VNPs to organelles intracellularly are challenges that will preoccupy researchers in this decade.
Fulltext Characteristics of human spermatozoa harvested in culture media with and without serum proteins

Ghofraan A. Ata’Allah, Noor Azmi Mat Adenan, Nuguelis Razali, Siti Khadijah Idris, Jaffar Ali, Kannappan, Palaniappan, et al.

Jurnal Sains Kesihatan Malaysia, 2018;16(1):125-134.
MyJurnal

This study was aimed to determine the efficiency of synthetic protein-free media in spermatozoa washing, preparation
and retention of the activity of washed spermatozoa over short periods in vitro. Normozoospermic semen samples (n =
71) were equally apportioned and washed using synthetic protein-free medium (PFM), minimum essential medium + HSA
(MEM) or commercial protein-containing medium (CPC). Washed spermatozoa were cultured in vitro using PFM, MEM or
CPC media and held for 24 hrs at 4°C, 15°C, 22°C or 37°C. Spermatozoa activity was evaluated at 0 hr, 4 to 7 hrs and
24 hrs post-wash. The effects of PFM on spermatozoa motility, vitality, membrane integrity and DNA fragmentation level
were not significantly different from that of MEM and CPC media at 0 hr, 4 to 7 hrs and 24 hrs post-wash in vitro. Synthetic
PFM, MEM and CPC retained spermatozoa activity highest when specimen were held at 22°C and it was significantly higher
(p < 0.05) than that at 37°C after 24 hrs incubation in vitro. However, no significant changes (p > 0.05) were noted
in spermatozoa DNA fragmentation (SDF) levels when specimen were held at 22°C or 37°C at 4 to 7 hrs and also after
24 hrs post-wash in vitro in all media. The use of synthetic PFM as an alternative to the commercial protein-containing
media in human spermatozoa washing and preparation procedure for an efficient and safer (Assisted Reproduction
Technology) ART outcome. Spermatozoa activity can be successfully retained at room temperature post-wash over short
periods; spermatozoa may lose viability rapidly if held for long hours at 37°C in all media.
Fulltext Knowledge, attitude and practice towards blood donation among health sciences students of Universiti Kebangsaan Malaysia

Nur Najmi Mohamad Anuar, Gan Wen Xin, Nurul Iman Natasya Zulkafali, Nur Aini Yusof, Muhamad Norizwan Jaffar Ali, Noor Idaya Salim, et al.

Life Sciences, Medicine and Biomedicine, 2020;4(1):0-0.
MyJurnal

Introduction: Increased demand and low blood supply have been a critical issue in many countries, including Malaysia. This issue occurs due to a lack of awareness towards the importance of blood donation. Therefore, this study aims to evaluate the level of knowledge, attitude and practice towards blood donation among undergraduate students of the Faculty of Health Sciences (FSK), Universiti Kebangsaan Malaysia (UKM), Kuala Lumpur. Methods: This cross-sectional study was conducted on undergraduate students of the Faculty of Health Sciences (FSK), Universiti Kebangsaan Malaysia (UKM), Kuala Lumpur, which is comprised of 9 programmes: Biomedical Science, Optometry, Nutrition Science, Dietetics, Audiology, Speech Science, Physiotherapy, Occupational Therapy and Environmental Health. The respondents were recruited by a stratified random sampling technique with an informed consent. Questionnaire that consisted of four sections, namely: (A) sociodemographic characteristics, (B) knowledge on blood donation, (C) attitude towards blood donation and (D) practice of blood donation were distributed and a total of 274 responses were obtained for further evaluation. The significance values were set as P < 0.05. Results: Our research findings showed that the majority of students had a good level of knowledge (99.6%) and attitude (95.3%) about blood donation but most of them demonstrated a poor practice (59.1%). As for genders, female students had a better level of knowledge and attitude than males, but male students committed better practice towards blood donation than females. However, there were no significant differences between gender and programme of study as the respondents shared a similar background in health-related courses. Conclusion: A majority of the students had a good level of knowledge and attitude, but they are poor in practice. More campaigns need to be conducted to create awareness on the importance of blood donation.