This study aimed to determine total antioxidant and antioxidant activity of selected local varieties
of lettuce (Lactuca sativa L.). Five varieties (iceberg, butterhead, romaine, green coral and
red coral) were subjected to DPPH radical scavenging activity and ferric reducing antioxidant
power assay (FRAP) assays for determination of antioxidant activity. Total phenolic content
and total flavonoid content were determined as total antioxidant. The EC50 values obtained from
the DPPH radical scavenging assay ranged from 303.56 to 4485.41 μg/ml. The red coral lettuce
had the lowest EC50 value indicating it possesses the highest antioxidant activity among the
varieties. This variety also showed the highest FRAP value compared with the other varieties,
where the values ranged from 48.05 to 2135.82 mM Fe2+/100 g fresh weight. Total phenolic
content of samples ranged from 4.85 to 76.05 mg gallic acid equivalent/100 g fresh weight,
with the red coral lettuce had the highest value. Total flavonoid content of the lettuce samples
ranged from 2.28 to 21.96 mg quercetin equivalent/100 g fresh weight, and were significantly
different (p