A total of 73 localities covering 4,894 premises and 26, 712 breeding habitats were surveyed in 1980 to determine and establish the density and distribution pattern of Aedes aegypti and Aedes albopictus in Sarawak. A similar pattern has been observed in the density of the Aedes aegypti and Aedes albopictus. The number of houses positive with Aedes larvae were found to be highest in the coastal areas followed by the inland rural areas. The Aedes aegypti Breteau Index (B.I.) of 0-525 in the coastal areas is the highest followed by 0-207.5 in the inland rural areas. The study undertaken has now revealed that both the Aedes aegypti and Aedes albopictus are widespread in the State.
An outbreak of Dengue and Dengue Harmorrhagic Fever occurred in Lawas District in 1983. A total of 134 cases were notified with 74 cases serologically confirmed. The epidemic which lasted for three months starting from week 20 and peaking in week 24 before being brought under control in week 35 is the first to occur in the district. At the end of the epidemic, 54 localities were affected starting from areas within the vicinity of the town before spreading further inland with the movement of the population.
Entomological investigation in all the infected areas revealed a high density of Aedes albopictus which was the sole vector present. Effective control of the epidemic was achieved through proper planning, active participation of various agencies and intensive outdoor spraying with malathion 2% or ULV concentrates.
An Aedes survey using various larval survey methods was conducted in 12 urban housing areas and 29 vacant lands in Sibu town proper. Aedes albopictus larvae were found in all areas surveyed while Aedes aegypti larvae were present in 10 localities and 4 vacant lands. There were no significant difference in the house index, breteau and larval density index of these two Aedes (Stegomyia) species from the survey areas. The proportion of containers positive with Ae. aegypti and Ae. albopictus in area outside the house compound and near the house fencing were 3.2 times higher than outdoor compound. The indoor/outdoor breeding ratio for Ae. aegypti alone is 1.6:1. The most preferred breeding habitats outdoor were plastic cups and used tires while indoor habitats were ant traps and flower vases. In the vacant lands, the average number of larvae per containers was significantly higher than in houses and over 51% of the containers inspected were positive. Shared breeding between Ae. aegypti and Ae. albopictus larvae accounted for 9% in house surveys and 4.5% in vacant land survey. The use of various methods in Aedes larval survey may provide essential information in the study of vector epidemiology in dengue and dengue hemorrhagic fever transmission.
The objective of this study was to elucidate the association of various risk factors with dengue cases reported in Lundu district, Sarawak, by analyzing the interaction between environmental, entomological, socio-demographic factors. Besides conventional entomological, serological and house surveys, this study also used GIS technology to generate geographic and environmental data on Aedes albopictus and dengue transmission. Seven villages were chosen based on the high number of dengue cases reported. A total of 551 households were surveyed. An overall description of the socio-demographic background and basic facilities was presented together with entomological and geographical profiles. For serological and ovitrap studies, systematic random sampling was used. Serological tests indicated that 23.7% of the 215 samples had a history of dengue, either recent or previous infections. Two samples (0.9%) were confirmed by IgM ELISA and 49 samples (22.8%) had IgG responses. A total of 32,838 Aedes albopictus eggs were collected in 56 days of trapping. Cluster sampling was also done to determine whether any of the risk factors (entomological or geographical) were influenced by geographical location. These clusters were defined as border villages with East Kalimantan and roadside villages along Lundu/Biawas trunk road. The data collected were analyzed using SPSS version 10.01. Descriptive analysis using frequency, means, and median were used. To determine the association between variables and dengue cases reported, and to describe the differences between the two clusters of villages, two-sample t-test, and Pearson's Chi-Square were used. Accurate maps were produced with overlay and density function, which facilitates the map visualization and report generating phases. This study also highlights the use of differential Global Positioning System in mapping sites of 1m accuracy. Analysis of the data revealed there are significant differences in clusters of villages attributable to container density, house density, distance of the house from the main road, and number of Ae. albopictus eggs from ovitraps set indoor, outdoor and in dumping sites (Person's Chi-Square = 6.111, df = 1, p < 0.01). Further analysis using t-test showed that house density, container density, indoor mosquitoes egg count, outdoor mosquitoes egg count, and dumping sites mosquitoes egg count were higher at the roadside villages compared to border villages. A number of potential risk factors including those generated from GIS were investigated. None of the factors investigated in this study were associated with the dengue cases reported.
Anopheles mosquitos were surveyed using three trapping technics in four longhouse settlements and their respectively farming zone in western Sarawak, Malaysia. The study area was mountainous with tropical rain forest. An. leucosphyrus and An. donaldi were predominant in the farm huts. An. tessellatus and An. subpictus were more abundant in the village settlements. In both ecotypes, human baited traps yielded a significantly greater proportion of Anopheles mosquito than CDC light traps and landing biting catches. Circumsporozoite antigen positively rate, mosquito survival rate and parasite rate showed that malaria transmission is more intense in farm huts than in longhouse settlements. The entomological inoculation rate of An. donaldi and An. leucosphyrus in farm huts was 0.035 and 0.023, respectively. No sporozoite infections were observed in the main settlements.