This study aims to investigate the influence of different stirring time for synthesis of silver nanoparticles in glutathione (GSH) aqueous solution. The silver nanoparticles (Ag-NPs) were prepared by green synthesis method using GSH as reducing agent and stabilizer, under moderate temperature at different stirring times. Silver nitrate (AgNO3) was taken as the metal precursor while Ag-NPs were prepared in the over reaction time.