A study was conducted to investigate the antiviral activity of aqueous extracts from Orthosiphon stamineus (OS).
Extraction was done using different parts of OS. The whole plant except root (WPOS), leaves (LOS) and flowers (FOS) of
OS were extracted using aqueous extraction method. Cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2,5-diphenyl
tetrazolium bromide (MTT) assay. Plaque reduction assays were carried out to evaluate the antiviral activity of OS extract
against herpes simplex virus type 1 (HSV-1). These include post-treatment, pre-treatment and virucidal assays. High
antiviral activity was observed in post-treatment and virucidal assay with 100% reduction of HSV-1 plaque at 0.39 mg/
mL in LOS, FOS and WPOS. In pre-treatment assay, 79%, 84% and 97% plaque reduction using the same concentration
was observed in FOS, LOS and WPOS, respectively. In conclusion, this study showed that OS aqueous extract has promising
potential to be explored as anti-HSV-1 agent regardless of the mode of treatment.