MyMedR

Displaying publications 21 - 40 of 91 in total

Abstract:

Sort:

Acute cassava poisoning in children in Sarawak

Cheok SS

Trop Doct, 1978 Jul;8(3):99-101.
PMID: 209589
Matched MeSH terms: Foodborne Diseases/diagnosis*; Foodborne Diseases/therapy
Fulltext Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Nordin N, Yusof NA, Radu S, Hushiarian R

J Vis Exp, 2018 06 03.
PMID: 29912194 DOI: 10.3791/56585

Vibrio parahaemolyticus (V. parahaemolyticus) is a common foodborne pathogen that contributes to a large proportion of public health problems globally, significantly affecting the rate of human mortality and morbidity. Conventional methods for the detection of V. parahaemolyticus such as culture-based methods, immunological assays, and molecular-based methods require complicated sample handling and are time-consuming, tedious, and costly. Recently, biosensors have proven to be a promising and comprehensive detection method with the advantages of fast detection, cost-effectiveness, and practicality. This research focuses on developing a rapid method of detecting V. parahaemolyticus with high selectivity and sensitivity using the principles of DNA hybridization. In the work, characterization of synthesized polylactic acid-stabilized gold nanoparticles (PLA-AuNPs) was achieved using X-ray Diffraction (XRD), Ultraviolet-visible Spectroscopy (UV-Vis), Transmission Electron Microscopy (TEM), Field-emission Scanning Electron Microscopy (FESEM), and Cyclic Voltammetry (CV). We also carried out further testing of stability, sensitivity, and reproducibility of the PLA-AuNPs. We found that the PLA-AuNPs formed a sound structure of stabilized nanoparticles in aqueous solution. We also observed that the sensitivity improved as a result of the smaller charge transfer resistance (Rct) value and an increase of active surface area (0.41 cm2). The development of our DNA biosensor was based on modification of a screen-printed carbon electrode (SPCE) with PLA-AuNPs and using methylene blue (MB) as the redox indicator. We assessed the immobilization and hybridization events by differential pulse voltammetry (DPV). We found that complementary, non-complementary, and mismatched oligonucleotides were specifically distinguished by the fabricated biosensor. It also showed reliably sensitive detection in cross-reactivity studies against various food-borne pathogens and in the identification of V. parahaemolyticus in fresh cockles.

Matched MeSH terms: Foodborne Diseases/diagnosis*; Foodborne Diseases/pathology
Impact of human Campylobacter infections in Southeast Asia: The contribution of the poultry sector

Premarathne JMKJK, Satharasinghe DA, Huat JTY, Basri DF, Rukayadi Y, Nakaguchi Y, et al.

Crit Rev Food Sci Nutr, 2017 Dec 12;57(18):3971-3986.
PMID: 28001082 DOI: 10.1080/10408398.2016.1266297

Campylobacter is globally recognized as a major cause of foodborne infection in humans, whilst the development of antimicrobial resistance and the possibility of repelling therapy increase the threat to public health. Poultry is the most frequent source of Campylobacter infection in humans, and southeast Asia is a global leader in poultry production, consumption, and exports. Though three of the world's top 20 most populated countries are located in southeast Asia, the true burden of Campylobacter infection in the region has not been fully elucidated. Based on published data, Campylobacter has been reported in humans, animals, and food commodities in the region. To our knowledge, this study is the first to review the status of human Campylobacter infection in southeast Asia and to discuss future perspectives. Gaining insight into the true burden of the infection and prevalence levels of Campylobacter spp. in the southeast Asian region is essential to ensuring global and regional food safety through facilitating improvements in surveillance systems, food safety regulations, and mitigation strategies.

Matched MeSH terms: Foodborne Diseases/microbiology; Foodborne Diseases/prevention & control*
Simulation of improper food hygiene practices: A quantitative assessment of Vibrio parahaemolyticus distribution

Malcolm TTH, Chang WS, Loo YY, Cheah YK, Radzi CWJWM, Kantilal HK, et al.

Int J Food Microbiol, 2018 Nov 02;284:112-119.
PMID: 30142576 DOI: 10.1016/j.ijfoodmicro.2018.08.012

Kitchen mishandling practices contribute to a large number of foodborne illnesses. In this study, the transfer and cross-contamination potential of Vibrio parahaemolyticus from bloody clams to ready-to-eat food (lettuce) was assessed. Three scenarios were investigated: 1) direct cross-contamination, the transfer of V. parahaemolyticus from bloody clams to non-food contact surfaces (hands and kitchen utensils) to lettuce (via slicing), was evaluated; 2) perfunctory decontamination, the efficacy of two superficial cleaning treatments: a) rinsing in a pail of water, and b) wiping with a kitchen towel, were determined; and 3) secondary cross-contamination, the microbial transfer from cleaning residuals (wash water or stained kitchen towel) to lettuce was assessed. The mean of percent transfer rates through direct contact was 3.6%, and an average of 3.5% of total V. parahaemolyticus was recovered from sliced lettuce. The attempted treatments reduced the transferred population by 99.0% (rinsing) and 94.5% (wiping), and the relative amount of V. parahaemolyticus on sliced lettuce was reduced to 0.008%. V. parahaemolyticus exposure via secondary cross-contamination was marginal. The relative amount of V. parahaemolyticus recovered from washed lettuce was 0.07%, and the transfers from stained kitchen towel to lettuce were insubstantial. Our study highlights that V. parahaemolyticus was readily spread in the kitchen, potentially through sharing of non-food contact surfaces. Results from this study can be used to better understand and potentially raising the awareness of proper handling practices to avert the spread of foodborne pathogens.

Matched MeSH terms: Foodborne Diseases/microbiology; Foodborne Diseases/prevention & control*
ENTEROAGGREGATIVE ESCHERICHIA COLI O104 FROM THAI AND IMPORTED MALAYSIAN RAW BEEF

Wameadesa N, Sae-lim A, Hayeebilan F, Rattanachuay P, Sukhumungoon P

Southeast Asian J Trop Med Public Health, 2017 Mar;48(2):338-50.
PMID: 29642296

Local Thai and imported Malaysian beef in southern Thailand area carry
several Shiga toxin-producing Escherichia coli (STEC) serotypes. STEC O104 is an
important pathogen capable of causing outbreaks with considerable morbidity
and mortality. This study investigated the presence of E. coli O104 from local Thai
and imported Malaysian beef obtained from markets in Hat Yai City, Songkhla
Province during August 2015 - February 2016. Thirty-one E. coli O104 strains
were isolated from 12 beef samples (16% and 23% Thai and imported Malaysian,
respectively). Thirty strains possessed aggA (coding for a major component of
AAF/I fimbriae), a gene associated with enteroaggregative E. coli (EAEC) pathotype,
and all strains carried fimH (encoding Type 1 fimbriae). Thirty strains
belonged to phylogenetic group B1 and one strain (from Malaysian beef) to group
A. Agglutination of yeast cells was observed among 29 E. coli O104 strains. Investigation
of stx2 phage occupancy loci demonstrated that sbcB was occupied in 12
strains. Antimicrobial susceptibility assay revealed that 7 strains were resistant
to at least one antimicrobial agent and two were multi-drug resistant. One strain
carried extended spectrum β-lactamase gene blaCTX-M and three carried blaTEM. PFGE-generated DNA profiling showed identical DNA pattern between that of
one EAEC O104 strain from Thai beef and another from Malaysian beef, indicating
that these two strains originated from the same clone. This is the first report
in Thailand describing the presence of EAEC O104 from both Thai and imported
Malaysian beef and their transfer between both countries. Thorough surveillance
of this pathogen in fresh meats and vegetables should help to prevent any possible
outbreak of E. coli O104.

Matched MeSH terms: Foodborne Diseases/microbiology; Foodborne Diseases/epidemiology*
Prevalence and antibiotic resistance of Campylobacter, Salmonella, and L. monocytogenes in ducks: a review

Adzitey F, Huda N, Ali GR

Foodborne Pathog Dis, 2012 Jun;9(6):498-505.
PMID: 22571641 DOI: 10.1089/fpd.2011.1109

Campylobacter, Salmonella, and Listeria monocytogenes are important bacterial pathogens associated with gastroenteritis. The consumption of poultry meat and their products is considered as a major and leading source of human infection. While surveys of chicken meat and products, and its association with foodborne pathogens are widely available, such information on ducks is scarce. This survey examines the prevalence and antibiotic resistance of Campylobacter, Salmonella and L. monocytogenes isolated from ducks. Data obtained from key surveys are summarized. The observed prevalence of these pathogens and their resistance to various antibiotics varies from one study to the other. The mean prevalence (and range means from individual surveys) are duck 53.0% (0.0-83.3%), duck meat and parts 31.6% (12.5-45.8%), and duck rearing and processing environment 94.4% (92.0-96.7%) for Campylobacter spp. For Salmonella spp., the mean prevalence data are duck 19.9% (3.3-56.9%), duck meat and parts 28.4% (4.4-75.6%), duck egg, shell, and content 17.5% (0-4.17%), and duck rearing and processing environment 32.5% (10.5-82.6%). Studies on the prevalence and antibiotic resistance of L. monocytogenes in ducks are by far very rare compared to Campylobacter and Salmonella, although ducks have been noted to be a potential source for these foodborne pathogens. From our survey, ducks were more frequently contaminated with Campylobacter than Salmonella. Campylobacter and Salmonella spp. also exhibited varying resistance to multiple antibiotics.

Matched MeSH terms: Foodborne Diseases/drug therapy; Foodborne Diseases/microbiology; Foodborne Diseases/prevention & control
Fulltext Draft Genome Sequences of Three Multiantibiotic-Resistant Campylobacter jejuni Strains (2865, 2868, and 2871) Isolated from Poultry at Retail Outlets in Malaysia

Teh AH, Lee SM, Dykes GA

Genome Announc, 2016;4(3).
PMID: 27151799 DOI: 10.1128/genomeA.00331-16

Campylobacter jejuni is a frequent cause of human bacterial gastrointestinal foodborne disease worldwide. Antibiotic resistance in this species is of public health concern. The draft genome sequences of three multiantibiotic-resistant C. jejuni strains (2865, 2868, and 2871) isolated from poultry at retail outlets in Malaysia are presented here.

Matched MeSH terms: Foodborne Diseases
Fulltext Knowledge, Attitude, and Practice toward Food Poisoning among Food Handlers and Dietetic Students in a Public University in Malaysia

Mohd Yusof AM, Rahman NAA, Haque M

J Pharm Bioallied Sci, 2018 12 21;10(4):232-239.
PMID: 30568381 DOI: 10.4103/JPBS.JPBS_141_18

Background: Food poisoning (FP) commonly occurs because of consuming contaminated food, which can be fatal. Many people are not aware of the dangers of FP. Thus, the purpose of this study is to analyze the knowledge, attitude, and practice (KAP) of FP among dietetic students (DS) and food handlers (FH) in a public university in Malaysia.
Materials and Methods: A cross-sectional study was designed, and a self-administered questionnaire was distributed to 106 respondents. The survey comprised four sections including sociodemographic, knowledge, attitude, and practice.
Results: Total percentage scores for KAP for FH were 86.06%, 32.40%, and 19.91%, respectively, whereas the KAP scores for DS were 89.36%, 34.26%, and 19.94%, respectively. This study revealed that the respondents had good knowledge but poor attitude and practice toward FP. Total mean percentage of KAP scores for DS was higher than FH. Besides, no significant difference was observed in KAP toward FP across different genders, age, education, and income levels among FH. However, for DS, significant difference (p = 0.008) was observed in knowledge toward FP between genders. Significant association (p = 0.048) was also reported in practice toward FP with age among DS. This study also found a significant association between knowledge and attitude (p = 0.032) and knowledge and practice (p = 0.017) toward FP among FH.
Conclusion: Nevertheless, among DS, no significant association was observed between knowledge, attitude and practice toward FP. The findings may help them to plan effective methods to promote better understanding about FP and improving their knowledge and awareness.

Matched MeSH terms: Foodborne Diseases
Fulltext Quantitative detection and characterization of Shiga toxin-producing Escherichia coli O157 and non-O157 in raw vegetables by MPN-PCR in Malaysia

Loo, Y. Y., Puspanadan, S., Goh, S. G., Kuan, C. H., Chang, W. S., Lye, Y. L., et al.

International Food Research Journal, 2013;20(6):3313-3317.
MyJurnal

Foodborne diseases are mainly caused by bacterial contamination which can lead to severe diarrhea. This study aimed to detect the presence of Shiga toxin-Producing Escherichia coli O157, Escherichia coli non-O157 and virulence gene in raw vegetables. The samples were purchased from wet market and hypermarket in Selangor. The detections were carried out by using the combination methods of Most Probable Number-Polymerase Chain Reaction (MPNPCR). A total of 37(18.5%) samples were found to be contaminated by STEC. Out of these 37 isolates, four (10.8%) of the isolates were E. coli O157 while 33(89.2%) were E. coli nonO157. However, there was no E. coli O157:H7 detected in all the samples. The occurrence of Shiga toxin-Producing E. coli in edible raw vegetables samples suggests the importance of this pathogen in vegetables. Therefore, more studies are required to remove this pathogen from vegetables.

Matched MeSH terms: Foodborne Diseases
Fulltext Food poisoning outbreak among Health Institute A staff 1st June 2010

Riana, K.A.H., Azahadi, O., Rohani, I., Zainol Ariffin, P., Maznieda, M., Siti Zubaidah, A.

Journal of Health Management, 2012;10(1):41-51.
MyJurnal

Purpose - An outbreak of food poisoning had occurred in Health Institute A on 1st June 2010 which involved twenty four staffs. Six of them were hospitalized and eighteen received outpatient treatment. A case control study was done to describe the epidemiological characteristics of the outbreak and to determine the source of infection.
Design/methodology/approach - Food samples, rectal swab from cases, environmental and hand swab from the food handlers were also taken and sent for microbiological analysis.
Finding- The outbreak was explosive, and the prominent clinical features were abdominal pain (62.2%), fever (51.4%), and diarrhea (56.8%). The onset time was between 9-24 hours and the median incubation period was at 15 hours. The possible source of the outbreak was durian gravy (OR= 14.00,95%CI: 2.72-92.55, p<0.001, food attack rate: 100%) and rotijala (OR= 6.69, 95%CI: LB5-24.23, p<0.001, food attack rate: 95%), The microbiological investigation revealed that various microorganisms including Salmonella spp, The food was supplied by the same food supplier from Caterer B since 2006 and the assessment of the kitchen showed that the cleanliness rate was only 54,3% with positive results of Coliform in all utensils and areas investigated in the kitchen.
Conclusion- In conclusion, the most probable causative organism based on signs and symptoms, epidemiological analysis and microbiological results was Salmonella spp. from contaminated food seryed during the ceremony. Education on food safety and food hygiene especially personal hygiene and hand washing should be emphasized continuously for prevention of future outbreak,

Matched MeSH terms: Foodborne Diseases
Fulltext Prevalence of pathogenic microorganims in ready-to-eat foods prepared at selected ‘high risk' school hostel kitchens and canteens in Pahang

Jeyaletchumi, P., Ardi, A, Noraliza, M.A., Nurul Aini, I., Maizatul Akmar, H., Suraya, H., et al.

Malaysian Journal of Public Health Medicine, 2006;6(1):23-28.
MyJurnal

Two hundred and sixty four samples of ready -to-eat foods (RTE) were obtained over a period of six months (April to September 2005) from 33 school hostel kitchens and canteens previously implicated in food poisoning outbreaks from 2000 to 2004. Sampling was done by food technologists and assistant environmental health ofhcers from various districts in Pahang while microbiological analysis was carried out at the Mentakab Food Quality Control Laboratory. The objective of the study was to obtain a comprehensive picture on the microbiological status of the foods that may have accounted for food poisoning outbreaks at school hostels and canteens in Pahang. Each food sample was analyzed for Total Plate Count (TPC), Salmonella, Coliform, Escheria coli, Staphylococcus aerus and Bacillus cereus. lt was found that none of the ready-to-eat foods sampled contained Salmonella although hve samples contained Bacillus cereus , four samples contained Staphylococcus aerus. High Coliform Counts were found in 15 food samples while Escheria coli was detected in two samples. Overall, it was found that 10.2 % of the samples had unsatisfactory counts.

Matched MeSH terms: Foodborne Diseases
Fulltext Evaluation of cloves (Syzygium aromaticum) against antibiotics resistant Vibrio parahaemolyticus on seafood

Elexson, N., Rukayadi, Y., Nakaguchi, Y., Nishibuchi, M.

International Food Research Journal, 2016;23(5):2229-2233.
MyJurnal

Vibrio parahaemolyticus is a main foodborne disease in seafood and generally seafood is
easily deteriorates in quality of color and flavor. In this study, clove (Syzygium aromaticum)
extract shows potent antibacterial activity against growth of antibiotics resistant Vibrio
parahaemolyticus on seafood samples (cockles and shrimps). Vibrio parahaemolyticus was
artificial contaminates on the samples with 106 CFU/ml. The samples were treated with different
concentration of cloves extract with 10 mg/ml which are 0.5%, 5% and 10% concentration
from methanol food grade extraction in 0 hr, 5 min, 10 min, 15 min, 30 min, 60 min and
120 min. Tab water and deionized water were selected as a negative control. As a result, the
amount of 10 % cloves managed to mitigates the number of V. parahaemolyticus on seafood
samples in 5 minutes and 15 min on both samples. Therefore, our results signify the fact that
cloves can be apply as natural sanitizer which could meet consumer demands for safe and
traditionally consumed either raw without any undesirable effect when applied in the seafood
system industries.

Matched MeSH terms: Foodborne Diseases
Fulltext An outbreak of typhoid from a wedding party in Bachok district, Kelantan, 2003

Wan Mansor, W.H., Hamizah, M.S., Wan Sulaili, W.S., Jeriah, I., Che Nok @ Nawi, I., Noraini, I., et al.

Malaysian Journal of Public Health Medicine, 2003;3(2):53-57.
MyJurnal

On March 17, 2003 the Kelantan Health Department was notihed about a possible typhoid outbreak following a wedding party. An investigation was carried out to identiy the source and recommend control measures. Active case detection, yield investigation and case»control study were conducted. Cases were symptomatic attendees with a stool or blood culture positive for Salmonella
typhii. Each control had a negative culture and denied symptoms. Of the more than 1 OOO guests, 477 experienced fever; 152 met the case definition. The party hostess was found to be an Salmonelb typhii carrier. Syrup prepared with untreated well water was identified as the most likely source for this outbreak, with an odds ratio 14.0 (95% C1: 2.9, 104.1). This was a common source
outbreak of typhoid. We recommend that all food handlers at large parties be screened for typhoid and other foodborne diseases and samples of higherisk foods should be kept for few days after each event in case they are needed for testing.

Matched MeSH terms: Foodborne Diseases
Fulltext Simultaneous multiplex Polymerase Chain Reaction detection of Salmonella spp., Escherichia coli O157, Vibrio parahaemolyticus, Vibrio cholerae, Listeria monocytogenes and Campylobacter spp.

Ling, S., Noramirah, R., Abidatul, A.A., Nurfarhanah, N.M.J., Noor-Azira, A.M., Jambari, N.N., et al.

Food Research, 2018;2(3):240-246.
MyJurnal

Foodborne illness is a global burden that impacts a country politically, economically and
socio-economically. The severity of the burden can be unmeasurable as foodborne illness
is often an underestimated problem. In order to enlighten the burden, appropriate food
safety control measures should be taken. This study aimed to optimize a multiplex
Polymerase Chain Reaction (mPCR) detection method to identify foodborne pathogens
simultaneously. Six foodborne pathogens namely, Salmonella spp., Escherichia coli O157,
Vibrio parahaemolyticus, Vibrio cholerae, Listeria monocytogenes and Campylobacter
spp., were targeted in the mPCR detection method. Each mPCR parameter was tested and
the outcome was analysed to obtain a successful mPCR protocol to detect the targeted
foodborne pathogens. The amplified PCR products showed that the optimized mPCR
protocol will be a potential rapid diagnostic tool in foodborne pathogen detection.

Matched MeSH terms: Foodborne Diseases
Fulltext The effectiveness of food handler training programmes in Malaysia and Ireland to prevent food-borne disease

Tirmizi, L.I.T., Brand, H., Son, R., New, C.Y.

Food Research, 2018;2(3):247-257.
MyJurnal

According to the World Health Organisation (WHO), globally 600 million people suffer
from food-borne diseases (FBD), and 420,000 people die as a result. The European Food
Safety Authority (EFSA) has stated that FBD are linked to the food industry, with the
most common means of transmission being due to poor food handling and hygiene by
food handlers working in the food industry. The aim of this research was to investigate the
effectiveness of mandatory food handler training programmes (FHTP) to prevent FBD in
Malaysia and Ireland. To do this, the FHTP existing in Malaysia and Ireland were
analysed, in addition to the legislation they fall under in each respective country.
Effectiveness was determined by investigating the level of food safety knowledge (FSK)
and food safety practices (FSP) of food handlers in Malaysia and Ireland. A systematic
literature review (SLR) and a narrative literature review (NLR) were conducted for this
research. The SLR was based on the PRISMA diagram, using the Confidence in the
Evidence from Reviews of Qualitative research (CERQual) approach to evaluate the
studies used for this research. A total of 8 Malaysian studies and 1 Irish study were used to
determine the level of FSK and FSP of food handlers in each respective country, to
examine the effectiveness of FHTP. The results of the studies used for this research have
depicted overall good FSP and FSK of food handlers in Malaysia and Ireland; yet trends
continue to show that food handlers are one of the biggest contributors to FBD,
demonstrating that FHTP are not effective in preventing FBD. The findings from this
research highlights that although these trainings can be an effective tool to prevent FBD, if
they are not executed correctly, food handlers will continue to contribute to FBD.

Matched MeSH terms: Foodborne Diseases
Fulltext Review article : detection of Listeria monocytogenes in foods

Jeyaletchumi, P, Tunung, R., Margaret, S.P, Son, R, Farinazleen, M.G., Cheah, Y.K

International Food Research Journal, 2010;17(1):1-11.
MyJurnal

Listeria monocytogenes is a gram positive, facultative intracellular pathogen with the capacity to cause
food poisoning outbreaks as well as severe illness in vulnerable human population groups. It can cause a rare but serious disease called listeriosis with high fatality rates (20–30%) compared with other foodborne microbial pathogens. Although Listeria monocytogenes is infective to all human population groups, it is more likely to cause severe problems among pregnant women, immunocompromised individuals, the elderly and neonates. There are a variety of phenotyphic and genotyphic methods for the detection of Listeria monocytogenes in foods. Recent technological advances have increased the ability of scientists to detect Listeria monocytogenes. The purpose of this review is to discuss molecular characteristics of the Listeria monocytogenes pathogen, standard detection methods of this pathogen in foods based on culture methods, confirmation of species and subtyping based on phenotypic and genotyphic methods.

Matched MeSH terms: Foodborne Diseases
Fulltext Presence of Bacillus cereus s.l. from ready-to-eat cereals (RTE) products in Sarawak

Lesley, M.B., Velnetti, L., Kasing, A., Samuel, L., Yousr, A.N.

International Food Research Journal, 2013;20(2):1031-1034.
MyJurnal

Bacillus cereus is a soil inhabitant gram positive bacterium, and is known to cause severe food poisoning. The objective of this study was to isolate and identify the presence of Bacillus cereus s.l. from selected ready to eat cereals purchased randomly from local supermarkets in Kuching and Kota Samarahan, Sarawak. The result showed that four of the 30 food samples were detected to be contaminated by B. cereus s.l. . Our findings suggested that it is important for the public to be aware of the safety of RTE cereals consumption, as it is possible that B. cereus s.l. may be present in high count number and pose hazardous health effects to the consumers.

Matched MeSH terms: Foodborne Diseases
Salmon poisoning disease in two Malayan sun bears

Gai JJ, Marks SL

J Am Vet Med Assoc, 2008 Feb 15;232(4):586-8.
PMID: 18279100 DOI: 10.2460/javma.232.4.586

2 captive sun bears (Helarctos malayanus) were evaluated because of acute onset of vomiting, mucoid diarrhea, lethargy, and anorexia 1 week after eating live trout from a northern California reservoir.

Matched MeSH terms: Foodborne Diseases/diagnosis; Foodborne Diseases/drug therapy; Foodborne Diseases/veterinary*
Determination of trans- and cis-urocanic acid in relation to histamine, putrescine, and cadaverine contents in tuna (Auxis Thazard) at different storage temperatures

Zare D, Muhammad K, Bejo MH, Ghazali HM

J Food Sci, 2015 Feb;80(2):T479-83.
PMID: 25586500 DOI: 10.1111/1750-3841.12752

Scombroid fish poisoning is usually associated with consumption of fish containing high levels of histamine. However, reports indicate that some cases have responded to antihistamine therapy while ingested histamine levels in these cases were low. Potentiation of histamine toxicity by some biogenic amines, and release of endogenous histamine by other compounds such as cis-urocanic acid (UCA) are some hypotheses that have been put forth to explain this anomaly. Very little is known about the effects of storage conditions on the production of both UCA isomers and biogenic amines in tuna. Thus, the production of trans- and cis-UCA, histamine, putrescine, and cadaverine in tuna during 15 d of storage at 0, 3, and 10 °C and 2 d storage at ambient temperature were monitored. The initial trans- and cis-UCA contents in fresh tuna were 2.90 and 1.47 mg/kg, respectively, whereas the levels of putrescine and cadaverine were less than 2 mg/kg, and histamine was not detected. The highest levels of trans- and cis-UCA were obtained during 15 d storage at 3 °C (23.74 and 21.79 mg/kg, respectively) while the highest concentrations of histamine (2796 mg/kg), putrescine (220.32 mg/kg) and cadaverine (1045.20 mg/kg) were obtained during storage at room temperature, 10 and 10 °C, respectively. Histamine content increased considerably during storage at 10 °C whereas trans- and cis-UCA contents changed slightly. The initial trans-UCA content decreased during storage at ambient temperature. Thus, unlike histamine, concentrations of trans- and cis-UCA did not result in elevated levels during storage of tuna.

Matched MeSH terms: Foodborne Diseases/pathology
Pyrosequencing analysis of microbial community and food-borne bacteria on restaurant cutting boards collected in Seri Kembangan, Malaysia, and their correlation with grades of food premises

Abdul-Mutalib NA, Amin Nordin S, Osman M, Ishida N, Tashiro K, Sakai K, et al.

Int J Food Microbiol, 2015 May 4;200:57-65.
PMID: 25679309 DOI: 10.1016/j.ijfoodmicro.2015.01.022

This study adopts the pyrosequencing technique to identify bacteria present on 26 kitchen cutting boards collected from different grades of food premises around Seri Kembangan, a city in Malaysia. Pyrosequencing generated 452,401 of total reads of OTUs with an average of 1.4×10(7) bacterial cells/cm(2). Proteobacteria, Firmicutes and Bacteroides were identified as the most abundant phyla in the samples. Taxonomic richness was generally high with >1000 operational taxonomic units (OTUs) observed across all samples. The highest appearance frequencies (100%) were OTUs closely related to Enterobacter sp., Enterobacter aerogenes, Pseudomonas sp. and Pseudomonas putida. Several OTUs were identified most closely related to known food-borne pathogens, including Bacillus cereus, Cronobacter sakazaki, Cronobacter turisensis, Escherichia coli, E. coli O157:H7, Hafnia alvei, Kurthia gibsonii, Salmonella bongori, Salmonella enterica, Salmonella paratyphi, Salmonella tyhpi, Salmonella typhimurium and Yersinia enterocolitica ranging from 0.005% to 0.68% relative abundance. The condition and grade of the food premises on a three point cleanliness scale did not correlate with the bacterial abundance and type. Regardless of the status and grades, all food premises have the same likelihood to introduce food-borne bacteria from cutting boards to their foods and must always prioritize the correct food handling procedure in order to avoid unwanted outbreak of food-borne illnesses.

Matched MeSH terms: Foodborne Diseases/prevention & control

Filters

Please provide feedback to Administrator ([email protected])

External Links