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Fulltext Language structure in Kelantan's flood situation from the perspective of Speech Act

Aswadi Mohamad, Zaitul Azma Zainon Hamzah

Malaysian Journal of Social Sciences and Humanities, 2018;3(3):17-25.
MyJurnal

This article focuses in structure of language within the village society in Manik Urai Kelantan during
the flood situation. The research was specifically to identify the structure of Speech Act use by the rural
community to give information about flood. Besides, this research observes the application of
linguistics methodology during flood situation using Speech Act Theory which is the categorization of
Speech Act by Searle (1969). This theory was choose for the ability to detail linguistic's differences
based on the structure of Speech Act categories such as representatives, directives, commissives,
expresives and declaratives. For the purpose of the research, a video about flood situation was choose
and transcribe to observe the the structure of speech act within the village society in the flood situation.
The result of the research shows all Speech Act have their structure. The beneficial of this research is as
a guideline and reference for the readers and language researchers especially those who were active
participant of volunteer field.

Matched MeSH terms: Volunteers
Electrodes for multifocal electroretinography (mfERG): A comparison of four electrodes types

Norhani Mohidin, Yap MK, Jacobs RJ

Sains Malaysiana, 2014;43:1089-1094.

The purpose of this study was to compare the performance of four different electrode types in detecting the multifocal electroretinogram (mfERG) using the visual evoked response imaging system (VERIS). Multifocal ERG of 30 healthy subjects aged 17-50 years was recorded. Four different types of electrodes were used (JET contact lens, gold foil, DTL thread and c-glide carbon fiber electrodes) and the trough to peak amplitude response densities of the first order kernels (which approximated to the a and b wave of the full field electroretinogram) were compared. The JET contact lens electrode produced the highest amplitude response which was significantly different from the gold foil, DTL thread and the c-glide electrodes, but there was no significant difference between the gold foil and DTL or between DTL and the c-glide electrodes. In conclusion, contact lens electrode produced the highest response density followed by the gold foil and the DTL thread. There was no significant difference in amplitude response between the gold foil and DTL thread, therefore these two electrodes provide for viable alternatives for recording mfERG especially when there are concerns that contact lens electrode may be uncomfortable for recording periods that may take a long time.

Matched MeSH terms: Healthy Volunteers
Transorbital alternating current stimulation modifies BOLD activity in healthy subjects and in a stroke patient with hemianopia: A 7 Tesla fMRI feasibility study

Sabel BA, Hamid AIA, Borrmann C, Speck O, Antal A

Int J Psychophysiol, 2020 08;154:80-92.
PMID: 30978369 DOI: 10.1016/j.ijpsycho.2019.04.002

BACKGROUND: Modifying brain activity using non-invasive, low intensity transcranial electrical brain stimulation (TES) has rapidly increased during the past 20 years. Alternating current stimulation (ACS), for example, has been shown to alter brain rhythm activities and modify neuronal functioning in the visual system. Daily application of transorbital ACS to patients with optic nerve damage induces functional connectivity reorganization, and partially restores vision. While ACS is thought to mainly modify neuronal mechanisms, e.g. changes in brain oscillations that can be detected by EEG, it is still an open question, whether and how it may alter BOLD activity.
OBJECTIVE: We evaluated whether transorbital ACS modulates BOLD activity in early visual cortex using high-resolution 7 Tesla functional magnetic resonance imaging (fMRI).
METHODS: In this feasibility study transorbital ACS in the alpha range and sham ACS was applied in a random block design in five healthy subjects for 20 min at 1 mA. Brain activation in the visual areas V1, V2 and V3 were measured using 7 Tesla fMRI-based retinotopic mapping at the time points before (baseline) and after stimulation. In addition, we collected data from one hemianopic stroke patient with visual cortex damage after ten daily sessions with 25-50 min stimulation duration.
RESULTS: In healthy subjects transorbital ACS increased the activated cortical surface area, decreased the fMRI response amplitude and increased coherence in the visual cortex, which was most prominent in the full field task. In the patient, stimulation improved contrast sensitivity in the central visual field. BOLD amplitudes and coherence values were increased in most early visual areas in both hemispheres, with the most pronounced activation detected during eccentricity testing in retinotopic mapping.
CONCLUSIONS: This feasibility study showed that transorbital ACS modifies BOLD activity to visual stimulation, which outlasts the duration of the AC stimulation. This is in line with earlier neurophysiological findings of increased power in EEG recordings and functional connectivity reorganization in patients with impaired vision. Accordingly, the larger BOLD response area after stimulation can be explained by more coherent activation and lower variability in the activation. Alternatively, increased neuronal activity can also be taken into account. Controlled trials are needed to systematically evaluate the potential of repetitive transorbital ACS to improve visual function after visual pathway stroke and to determine the cause-effect relationship between neural and BOLD activity changes.

Matched MeSH terms: Healthy Volunteers
Fulltext Bilateral Heschl’s Gyrus display non-reciprocity in connectivity during the performance of simple arithmetic addition task

Yusoff, A.N., Te, L.H., Mukari, S.Z.M., Abd Hamid, A.I.

Jurnal Sains Kesihatan Malaysia, 2016;14(1):37-45.
MyJurnal

Heschl’s gyrus (HG) is known to interact with other auditory related areas of the same hemisphere during the performance
of an auditory cognitive task. However, the information about how it interacts with the opposite HG is still lacking.
The aim of this study was to investigate the psychophysiologic interaction (PPI) between the bilateral HG during a
simple arithmetic addition task and to verify the role of noise as an experimental factor that would modulate the PPI.
Functional magnetic resonance imaging (fMRI) scans were performed on eighteen healthy participants, in which a
single-digit addition task were solved during in-quiet (AIQ) and in-noise (AIN) conditions. The fMRI data were analysed
using Statistical Parametric Mapping (SPM8). The interaction between the bilateral HG was investigated using PPI
analysis. The response in right HG was found to be linearly influenced by the activity in left HG, vice-versa, for both
in-quiet and in-noise conditions. The connectivity from right to left HG in noisy condition seemed to be modulated
by noise, while the modulation is relatively small oppositely, indicating a non-reciprocal behavior. A two-way PPI
model between right and left HG is suggested. The connectivity from right to left HG during a simple addition task in
noise is driven by a higher ability of right HG to perceive the stimuli in a noisy condition. Both the bilateral HGs took
part in the cognitive processes of arithmetic addition from which the interactions between the two were found to be
different in noise.

Matched MeSH terms: Healthy Volunteers
Fulltext Resting-state electroencephalography (EEG) microstates of healthy individuals following mild sleep deprivation

Khoo SY, Lai WH, On SH, On YY, Adam BM, Law WC, et al.

Sci Rep, 2024 Jul 22;14(1):16820.
PMID: 39039219 DOI: 10.1038/s41598-024-67902-0

Mild sleep deprivation is widespread in many societies worldwide. Electroencephalography (EEG) microstate analysis provides information on spatial and temporal characteristics of resting brain network, serving as an indicator of neurophysiological activities at rest. This study seeks to investigate potential neural markers in EEG following mild sleep deprivation of a single night using EEG microstate analysis. Six-minute resting EEG was conducted on thirty healthy adults within 6 hours of waking in the morning and after at least 18 h of sleep deprivation. Translated and validated Malay language Karolinska Sleepiness Scale was used to assess the participants' degree of sleepiness. Microstate characteristics analysis was conducted on the final 24 subjects based on four standard microstate maps. Microstate C shows a significant increase in mean duration, coverage and occurrence, while microstate D has significantly higher occurrence after sleep deprivation. This study demonstrates notable changes in resting state EEG microstates following mild sleep deprivation. Present findings deepen our understanding of the brain's spatiotemporal dynamics under this condition and suggest the potential utility of neural markers in this domain as components of composite markers for sleep deprivation.

Matched MeSH terms: Healthy Volunteers
Fulltext Finger clubbing: do we require digital index quantitator?

Djojodibroto RD, Thomas PT, Kana KT, Hla M

Med J Malaysia, 2014 Apr;69(2):60-3.
PMID: 25241813 MyJurnal

Some diseases may underlie finger clubbing. However, there is a dearth of information about early stage of finger clubbing because only few researchers have shown interest in it. We determined the Digital Index of normal, healthy subjects by using thread and manual Vernier calipers, the time used for the procedure, and its interrater reliability. The value of Digital Index was 8.86 ± 0.29 (Mean ± SD) with a range of 8.15 to 9.41. Interrater reliability was excellent with Pearson's correlation coefficient of 0.966. Overall, the time taken to measure the Digital Index ranged from 21.93 to 68.80 minutes with an average of 35.97 ± 9.16 (Mean ± SD). Determining Digital Index need much time, but this can be overcome if we use Digital Index Quantitator (DIQ). Availability of DIQ in the hospital wards will be of much benefit. DIQ can also be used to accurately quantify the progression or regression of the clubbing process. This article proves that we need morphometry of digital clubbing as well as the correlation of the physical sign of clubbing with Digital Index.

Matched MeSH terms: Healthy Volunteers
Fulltext Preliminary study on formulation of pvabased face mask incorporated with sugarcane bagasse microfibrillated cellulose

Dzun Noraini Jimat, Istisyhad Mohamad, Azura Amid, Azlin Suhaida Azmi, Mohd Firdaus Abd. Wahab

Biological and Natural Resources Engineering Journal, 2020;3(1):7-13.
MyJurnal

Cellulose facial masks have gained a huge interest in the cosmetic industry. Cellulose can be extracted from plant biomass, bacteria and algae. In this study, several formulated PVA-based facial masks (F1, F2, F3, F4) incorporated with microfibrillated cellulose extracted from sugarcane bagasse (MFC-SCB) were prepared. The concentration of polyvinyl alcohol (PVA) was varied (5%–20% (w/w)) while the concentration of microfibrillated cellulose of sugarcane bagasse (MFC-SCB) was fixed at 5% (w/w) to get the appropriate composition of the facial masks. The MFC-SCB was
extracted through chemical treatment assisted with ultrasonication. Sensory tests in terms of adhesion to the skin, spreadability, color, odor, and drying time were performed. These tests were carried out by requesting the volunteers to rate the performance of the masks. The results showed that the formulated facial mask F3 (15% [w/w] of PVA and 5% [w/w] of MFC-SCB) has the highest average score (13.9) which is 82% from the total score compared to other formulated masks. However, the standard formulation mask F5 (15% [w/w] of PVA and 5% [w/w] of sodium carboxylmethyl cellulose, CMC) achieved the highest score (13.5) compared to F3 (12.5). The findings of this study proved that the presence of MFC-SCB with PVA has a competitive performance with the standard facial mask formulation.

Matched MeSH terms: Volunteers
Fulltext Effects of Stochastic Facilitation in Healthy Subjects with Different Auditory Working Memory Capacity

Othman, E. A., Mohamad, M., Abdul Manan, H., Yusoff, A. N.

Physics and Technology in Medicine, 2020;1(2):27-31.
MyJurnal

This study investigated the effects of stochastic facilitation in healthy subjects with normal and low auditory working memory capacity (AWMC). Forty healthy volunteers were recruited in this study. They performed a backward recall task (BRT) in quiet and under four white noise intensity levels: 45, 50, 55, and 60 dB. Brain activations during the task were measured using functional magnetic resonance imaging (fMRI). The behavioral performance in both groups increased significantly in 50 and 55 dB white noise. The normal AWMC group (mean score = 48.70) demonstrated higher activation in the superior temporal gyrus and prefrontal cortex than the low AWMC group (mean score = 30.85). However, comparisons in the brain activation between groups for all noise levels were not statistically different. The results support previous findings that stochastic facilitation enhances cognitive performance in healthy individuals. The results also proposed that brain activity among healthy subjects is more or less similar, at least in the context of auditory working memory. These findings indicated that there were no differential effects of stochastic facilitation in healthy subjects with different AWMC.

Matched MeSH terms: Healthy Volunteers
Fulltext Effects of tocotrienol supplementation on hair growth in human volunteers

Beoy LA, Woei WJ, Hay YK

Trop Life Sci Res, 2010 Dec;21(2):91-9.
PMID: 24575202 MyJurnal

Studies have shown an association between oxidative stress and alopecia. Patients with alopecia generally exhibit lower levels of antioxidants in their scalp area as well as a higher lipid peroxidation index. Tocotrienols belong to the vitamin E family and are known to be potent antioxidants. Hence, a study was conducted to investigate the effect of tocotrienol supplementation on hair growth in volunteers suffering from hair loss. Twenty one volunteers were randomly assigned to orally receive 100 mg of mixed tocotrienols daily while 17 volunteers were assigned to receive placebo capsule orally. The volunteers were monitored for the number of hairs in a pre-determined scalp area as well as the weight of 20 strands of 1 cm length hair clippings at 0 (before supplementation), 4 and 8 months. The number of hairs of the volunteers in the tocotrienol supplementation group increased significantly as compared to the placebo group, with the former recording a 34.5% increase at the end of the 8-month supplementation as compared to a 0.1% decrease for the latter. Nevertheless, the cumulative weight of 20 strands of hair clippings did not differ much from the baseline for both supplementation groups at the end of the study period. In conclusion, this trial demonstrated that supplementation with tocotrienol capsules increases hair number in volunteers suffering from hair loss as compared to the placebo group. This observed effect was most likely to be due to the antioxidant activity of tocotrienols that helped to reduce lipid peroxidation and oxidative stress in the scalp, which are reported to be associated with alopecia.

Matched MeSH terms: Volunteers
Fulltext Differential effects of minified and magnified mirror visual feedback on the underlying misperception of hand size

Perera AT, Tan JT, Mu PS, Newport R

Atten Percept Psychophys, 2021 Jul;83(5):2281-2290.
PMID: 33754299 DOI: 10.3758/s13414-021-02262-9

Perception of the size of body parts, for instance the hand, has been shown to be distorted in healthy participants, with over- and underestimations of width and length, respectively. Illusory manipulations of body shape and size have highlighted the flexibility of the body representation and have also been found to update immediate perceptions of body size and surrounding objects. Here, we examined whether underlying misperceptions of hand width and length can be modified through exposure to illusory changes in hand size using a mirror visual feedback (MVF) paradigm. While questionnaire responses indicated subjective susceptibility to both magnified and minified manipulations, objective hand size estimates only showed significant differences following exposure to minifying mirrors. These variations might reflect differences in the way that stored representations are accessed or updated in response to size manipulations. Secondly, the findings further reinforce differences between subjective and objective outcomes of illusions on subsequent body perception.

Matched MeSH terms: Healthy Volunteers
Fulltext Determining the effectiveness of the different preparation protocols for platelet-rich plasma (PRP) in yielding higher concentrations of platelets

Kavitha, G., Sangeetha, V.N., Shani, S., Murali, M.R., Raja, E.A., Rukmanikanthan, S., et al.

JUMMEC, 2011;14(2):1-6.
MyJurnal

INTRODUCTION: Despite the various methods described in producing platelet-rich plasma (PRP), it is well established that this biological product in its many preparations have been proven to enhance wound healing. However, very little have been known about the efficacy of these methods hence there is a lack of evidence in the superiority of one method over another. Thus, a study was conducted to compare these different protocols to determine which produces the highest concentration of platelets.
METHODS: Peripheral blood was obtained from 24 healthy volunteers. Four different protocols using similar 2 step centrifugation methods of preparing PRP were applied to an equal number of samples in this study. Platelet counts were performed on whole blood (without processing), PRP preparations and platelet-poor plasma (PPP).
RESULTS: All protocols produced higher amounts of platelet concentrates in PRP preparations than plasma. However, centrifugation at 150g for 10 minutes followed by another at 450g at 10 minutes produces significantly higher amount of platelets concentration (p<0.05)
CONCLUSION: Optimizing the protocols to produce PRP appears to be important in obtaining a maximal yield of platelet concentrate. Here the protocol described has shown to provide significant concentration yield over all others.
Keywords: platelet-rich-plasma, growth factors, centrifugal forces

Matched MeSH terms: Healthy Volunteers
Fulltext National Malaysian twin registry - a perfect opportunity for researchers to study nature versus nurture

Jahanfar, S.

Malays Fam Physician, 2008;3(2):111-112.
PMID: 25606132 MyJurnal

A twin registry is a registry of twin pairs (monozygotic = MZ and Dizygotic = DZ) who are willing to consider participating in health-related research. Twins are able to help researchers study the impact of genetic and environmental factors on health and the treatment and prevention of disease in a special way. Throughout the world, twin registries have been established by the governments via the National Health and Medical Research in order to put researchers in touch with twins who might be willing to take part in particular projects. In Australia, for example, more than 30,000 pairs of twins have joined the registry, making it the largest volunteer registry of its kind in the world. However, in some countries such as Denmark, the first twin registry was born in a medical faculty and it was then expanded to the National Twin Registry of Denmark. (Copied from article).

Matched MeSH terms: Volunteers
Fulltext The impact of geographical location on taste sensitivity and preference

Baharuddin, A.R, Sharifudin, M.S.

International Food Research Journal, 2015;22(2):731-738.
MyJurnal

This study was carried out to determine recognition threshold and taste preference for three basic tastes (sweetness, saltiness and sourness) based on location (interior and coastal) among the Kadazandusun ethnic in Sabah, Malaysia. One hundred and ninety four (194) volunteers aged 20 to 55 years were selected randomly (stratified) as subject. Three Alternative Forced- Choice (3AFC) and hedonic test were used to determine the taste threshold and preference. The interior group had lower taste threshold for all tastes; sweet (10.97 g/L ± 3.69), salty (1.14 g/L ± 0.38), sour (0.0095 g/L ± 0.011) compared to the coastal group; sweet (11.56 g/L ± 3.71), salty (1.23 g/L ± 0.39), sour (0.0012 g/L ± 0.0034). For intensity and hedonic rating, the patterns of response varied based on location for sweet and sour taste. No significant different (p>0.05) was observed for salty taste. However, both groups preferred the base stimulus which concentration similar to the commercially available products tested. There is a correlation between taste threshold and optimum concentration. Individuals with preferred high taste intensity tend to have higher taste threshold. Location and culture can influenced individual taste preference. However, exposure and experience to taste sensation was the major factor on individual’s taste preference.

Matched MeSH terms: Volunteers
Fulltext The COMBI (Communication for Behavioural Impact ) program in the prevention and control of dengue - the Hulu Langat experienc

Rozhan, S., Jamsiah, M., Rahimah, A., Ang, K.T.

Malaysian Journal of Community Health, 2006;12(1):0-0.
MyJurnal

Background: The COMBI concept is a novel approach by the WHO to control communicable diseases which are influenced by community behaviour. The Ministry of Health is currently evaluating its use against dengue in selected areas throughout Malaysia. COMBI doctrine differs from previous dengue campaigns. It acknowledges that the factors contributing to dengue proliferation may differ between areas. Factors for a given area are analysed, then a single precise behavioural goal to overcome those problems is formulated. To inculcate this behavioural change, the target community is subjected to an intensive campaign using Integrated Marketing Communication techniques adapted from the advertisingindustry, particularly involving volunteers from the community itself.
Methodology: In Selangor the pilot project was implemented in Section 3 and Section 4 of Bandar Baru Bangi, in the district of Hulu Langat. Here, Aedes breeding was found to occur mainly in water containers of semi permanent nature (eg. ‘kolah’, aquatic plant jars, flower pot bases etc). A total of 172 volunteers were recruited to disperse the message of “Suluh – Suluh, Basuh - Basuh” whilst distributing leaflets and flashlights to 2666 homes. Residents were instructed to illuminate such water containers twice weekly and scrub any containers found to contain larvae. The program commenced on 23/5/2004 and lasted 16 weeks.
Results : During this period, the initial Aedes Index of 5 was reduced to 0.96 while combined cases of Dengue Fever / Dengue Haemorraghic Fever in Sections 3 and 4 reported to the Hulu Langat District Health Office also dropped to 1 (unconfirmed).
Conclusion : The COMBI approach in Hulu Langat successfully demonstrated that correct problem identification synergized with community engagement can potentially reduce Aedes proliferationand dengue morbidity.

Matched MeSH terms: Volunteers
Fulltext A Study on the Absence of Palmaris Longus in a Multiracial Population

Roohi, S.A., Choon-Sian, L, Shalimar, A., Tan,G.H., Naicker, A.S.

Malays Orthop J, 2007;1(1):26-28.
MyJurnal

Palmaris longus is a dispensable muscle with a long tendon which is very useful in reconstructive surgery. It is absent 2.8 to 24% of the population depending on the race/ethnicity studied. Four hundred and fifty healthy subjects (equally distributed among Malaysia's 3 major ethnic groups) were clinically examined for the presence or absence of palmaris longus. This tendon was found to be absent unilaterally in 6.4% of study subjects, and bilaterally in 2.9% of study participants. Malays have a high prevalence of palmaris longus absence at 11.3% followed closely by Indians at 10.7% whilst Chinese had a low absence rate of 6.0%.

Matched MeSH terms: Healthy Volunteers
Fulltext Revitalizing community and family case study (CFCS) programme

Fahisham Taib, Nik Mohd Rizal Mohd Fakri

Education in Medicine Journal, 2014;6(3):46-50.
MyJurnal

Community and family case study (CFCS) is a community based educational (CBE) programme commenced in 1980 at School of Medical Sciences, Universiti Sains Malaysia. It serves to help and resolve health needs of the individual patients, family and their community. This will help to develop generic skills such as organization, communication and problem solving in relation to local norms, beliefs and socio-cultural influences. Students should have good awareness, sensitivity and empathic response based on health needs and environment that they are living in. Knowledge, skills, attitudes, emotions and values are repeatedly exposed to inculcate optimal learning environment with the community, as well as triggered response by the student to give back to the community by volunteerism. CFCS activism can be revitalized within the individual and group context. This can be achieved by multi-dimensional learning, collaborative effort, effective leadership and supervision, creative programme initiation, effectual research activities and sustainable service to the community. It is hopeful with active community engagement, life experiences learning process, self-directed and reflective learning, students are able to acquire refined attributes such as skills, behaviour, knowledge and attitudes, towards successful personal growth and advancement.

Matched MeSH terms: Volunteers
Fulltext Palm kernel oil blends as suppository bases in the delivery of aspirin

Noordin, M.I., Chung, L.Y.

JUMMEC, 2007;10(2):43-50.
MyJurnal

Rectal delivery of drugs has been proven to be effective in terms of drug absorption and distribution comparable with other routes such as oral, buccal, sublingual or even nasal. In this study, two new suppository bases were developed using combinations of locally sourced hydrogenated palm kernel oil, hydrogenated palm kernel stearin and hydrogenated palm kernel olein with mixtures of stearic acid and glyceryl monostearate. When formulated with aspirin, these bases produced suppositories with acceptable characteristics. These aspirin suppositories were tested on twelve healthy subjects after an approval from the Medical Ethics Committee, University of Malaya had been procured. We quantified aspirin from the urine samples of the subjects to determine the relative availability of the different suppository preparations relative to an oral dose. The excretion of salicylic acid, one of the metabolite of aspirin in human urine taking aspirin was quantified. The F value was found to range from 1.16 to 1.38. Hence, the excretion results showed that these palm kernel oil blends are suitable suppository bases.

Matched MeSH terms: Healthy Volunteers
Fulltext Operational utility of the reverse-transcription recombinase polymerase amplification for detection of dengue virus

Tan KK, Azizan NS, Yaacob CN, Che Mat Seri NAA, Samsudin NI, Teoh BT, et al.

BMC Infect Dis, 2018 04 11;18(1):169.
PMID: 29642856 DOI: 10.1186/s12879-018-3065-1

BACKGROUND: A method for rapid detection of dengue virus using the reverse-transcription recombinase polymerase amplification (RT-RPA) was recently developed, evaluated and made ready for deployment. However, reliance solely on the evaluation performed by experienced researchers in a well-structured and well-equipped reference laboratory may overlook the potential intrinsic problems that may arise during deployment of the assay into new application sites, especially for users unfamiliar with the test. Appropriate assessment of this newly developed assay by users who are unfamiliar with the assay is, therefore, vital.
METHODS: An operational utility test to elucidate the efficiency and effectiveness of the dengue RT-RPA assay was conducted among a group of researchers new to the assay. Nineteen volunteer researchers with different research experience were recruited. The participants performed the RT-RPA assay and interpreted the test results according to the protocol provided. Deviation from the protocol was identified and tabulated by trained facilitators. Post-test questionnaires were conducted to determine the user satisfaction and acceptability of the dengue RT-RPA assay.
RESULTS: All the participants completed the test and successfully interpreted the results according to the provided instructions, regardless of their research experience. Of the 19 participants, three (15.8%) performed the assay with no deviations and 16 (84.2%) performed the assay with only 1 to 5 deviations. The number of deviations from protocol, however, was not correlated with the user laboratory experience. The accuracy of the results was also not affected by user laboratory experience. The concordance of the assay results against that of the expected was at 89.3%. The user satisfaction towards the RT-RPA protocol and interpretation of results was 90% and 100%, respectively.
CONCLUSIONS: The dengue RT-RPA assay can be successfully performed by simply following the provided written instructions. Deviations from the written protocols did not adversely affect the outcome of the assay. These suggest that the RT-RPA assay is indeed a simple, robust and efficient laboratory method for detection of dengue virus. Furthermore, high new user acceptance of the RT-RPA assay suggests that this assay could be successfully deployed into new laboratories where RT-RPA was not previously performed.

Matched MeSH terms: Volunteers
Molecular Detection of Porphyromonas gingivalis in Chronic Periodontitis Patients

Kulkarni PG, Gosavi S, Haricharan PB, Malgikar S, Mudrakola DP, Turagam N, et al.

J Contemp Dent Pract, 2018 Aug 01;19(8):992-996.
PMID: 30150503

AIM: In the current study, Porphyromonas gingivalis was identified in chronic periodontitis patients and healthy subjects by polymerase chain reaction (PCR) and its presence correlated with the severity of clinical periodontal parameters.
MATERIALS AND METHODS: Subgingival plaque samples were collected with sterile curette and subjected to deoxyribonucleic acid (DNA) extraction and subsequent PCR for detection of P. gingivalis.
RESULTS: Porphyromonas gingivalis was detected in 60% of patients of group II (pocket depth up to 5 mm), and in 93.33% of patients of group III (pocket depth more than 5 mm). One periodontally healthy subject in group I (probing depth < 3 mm) showed the presence of P. gingivalis.
CONCLUSION: Detection frequency of bacterium increased significantly with increase in probing pocket depth (PPD), loss of attachment (LOA), and gingival index (GI).
CLINICAL SIGNIFICANCE: Porphyromonas gingivalis is strongly associated with chronic periodontitis and its detection frequency positively correlates with the severity of periodontal destruction.

Matched MeSH terms: Healthy Volunteers
Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens

Azizah Ahmad Fauzi, Zaleha Shafiei, Badiah Baharin, Nurulhuda Mohd

Sains Malaysiana, 2013;42:19-24.

Bacteriocin or Bacteriocin like inhibitory substances (BLIS) is a protein antibiotic that has a relatively narrow spectrum of killing activity. It could potentially serve as a natural alternative to antibiotics in reducing the development of multi-drug resistant bacteria. Antimicrobial activity of the strains of Lactobacillus sp. isolated from healthy subjects (test strains) against Aggregatibacter actinomycetemcomitans and other periodontal pathogens (indicator strains) isolated from subgingival plaques of aggressive periodontitis patients were determined by using deferred antagonism test and agar-well diffusion method. Strains of Lactobacillus sp., Aggregatibacter actinomycetemcomitans and black pigmented bacteria were selectively isolated from TJA, TSBV and TSBA agars, respectively. Mean diameter zone of inhibition of at least 10 mm was considered as positive results for both methods. Out of 25 strains of Lactobacillus sp. screened, only eight test strains of Lactobacillus sp. showed the specific antimicrobial activity against certain strains of indicator periodontal pathogens during deferred antagonism test. However, out of eight potential strains, only three strains, which were Lactobacillus sp. strain S, Lactobacillus sp. strain V and Lactobacillus sp. strain W consistently showed positive inhibitory activity against black pigmented bacteria by deferred antagonism test and agar-well diffusion method. Therefore, these three strains should be considered as potential BLIS producer strains for further study.

Matched MeSH terms: Healthy Volunteers

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