Displaying publications 21 - 40 of 942 in total

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  1. Fulltext Falsarthrobacter nasiphocae periprosthetic joint infection
    Tay ST, Merican AM, Abdul Jabar K, Velayuthan RD, Ayob KA, Lee JL, et al.
    Int J Infect Dis, 2023 Nov;136:77-80.
    PMID: 37660726 DOI: 10.1016/j.ijid.2023.08.025
    We report the isolation of a rare Gram-positive coccobacillary bacterium from synovial fluids of a patient with periprosthetic joint infection on three occasions over an 8-month period. As routine microbiological methods were not able to identify the isolate definitely, sequence analyses of the bacterial 16S ribosomal RNA gene and whole genome were performed. Analysis of the bacterial 16S ribosomal RNA gene showed the highest similarity (98.1%) with that of Falsarthrobacter (previously known as Arthrobacter) nasiphocae, which was first isolated from the nasal cavities of common seals (Phoca vitulina). The genome size of the strain (designated as UM1) is 2.4 Mb. With a high G+C content (70.4 mol%), strain UM1 is phylogenetically most closely related to F. nasiphocae based on whole genome analysis. Strain UM1 was susceptible to vancomycin, linezolid, trimethoprim-sulfamethoxazole, doxycycline, and intermediate to penicillin and ciprofloxacin. Ceftriaxone resistance was noted. The patient who was also on hemodialysis for his end stage kidney disease died approximately 3 weeks following implant removal and fusion with an external fixator. This study describes the first isolation of F. nasiphocae from human clinical samples. The use of emerging technologies has supported more definitive etiological diagnosis associated with rarely encountered organisms in periprosthetic joint infection.
    Matched MeSH terms: Bacteria; Gram-Positive Bacteria
  2. Investigating the crude oil biodegradation performance in bioreactor by using a consortium of symbiotic bacteria
    Chuah LF, Nawaz A, Dailin DJ, Oloruntobi O, Habila MA, Tong WY, et al.
    Chemosphere, 2023 Oct;337:139293.
    PMID: 37369285 DOI: 10.1016/j.chemosphere.2023.139293
    Crude oil pollution is one of the most serious environmental issues today, and the clean-up procedure is perhaps the most difficult. Within one to three weeks, the vast majority of oil bacteria may degrade approximately 60% of the crude oil, leaving approximately 40% intact. The by-product metabolites produced during the breakdown of oil are essentially organic molecules in nature. These metabolites inhibit its enzymes, preventing the oil bacteria from further degrading the oil. By combining a variety of different oils with heterotrophic bacteria in a bioreactor, the rate of crude oil biodegradation was accelerated. In this study, two strains of oil-resistant, heterotrophic bacteria (OG1 and OG2-Erythrobacter citreus) and a bacterium that uses hydrocarbons (AR3-Pseudomonas pseudoalcaligenes) were used. Gas chromatography-mass spectroscopy was used to investigate the effectiveness of this consortium of symbiotic bacteria in the biodegradation of crude oil. According to gravimetric and gas chromatography analyses, the consortium bacteria digested 69.6% of the crude oil in the bioreactor, while the AR3 single strain was only able to destroy 61.9% of it. Under the same experimental conditions, consortium bacteria degraded approximately 84550.851 ppb (96.3%) of 16 aliphatic hydrocarbons and 9333.178 ppb (70.5%) of 16 aromatic hydrocarbons in the bioreactor. It may be inferred that the novel consortium of symbiotic bacteria accelerated the biodegradation process and had great potential for use in increasing the bioremediation of hydrocarbon-contaminated locations.
    Matched MeSH terms: Bacteria/metabolism
  3. Fulltext Plant- vs. Bacterial-Derived Cellulose for Wound Healing: A Review
    Naomi R, Bt Hj Idrus R, Fauzi MB
    Int J Environ Res Public Health, 2020 Sep 18;17(18).
    PMID: 32961877 DOI: 10.3390/ijerph17186803
    Cellulose is a naturally existing element in the plant's cell wall and in several bacteria. The unique characteristics of bacterial cellulose (BC), such as non-toxicity, biodegradability, hydrophilicity, and biocompatibility, together with the modifiable form of nanocellulose, or the integration with nanoparticles, such as nanosilver (AgNP), all for antibacterial effects, contributes to the extensive usage of BC in wound healing applications. Due to this, BC has gained much demand and attention for therapeutical usage over time, especially in the pharmaceutical industry when compared to plant cellulose (PC). This paper reviews the progress of related research based on in vitro, in vivo, and clinical trials, including the overall information concerning BC and PC production and its mechanisms in wound healing. The physicochemical differences between BC and PC have been clearly summarized in a comparison table. Meanwhile, the latest Food and Drug Administration (FDA) approved BC products in the biomedical field are thoroughly discussed with their applications. The paper concludes on the need for further investigations of BC in the future, in an attempt to make BC an essential wound dressing that has the ability to be marketable in the global marketplace.
    Matched MeSH terms: Bacteria*
  4. Stimulating bioelectric generation and recovery of toxic metals through benthic microbial fuel cell driven by local sago (Cycas revoluta) waste
    Daud NNM, Al-Zaqri N, Yaakop AS, Ibrahim MNM, Guerrero-Barajas C
    Environ Sci Pollut Res Int, 2024 Mar;31(12):18750-18764.
    PMID: 38349489 DOI: 10.1007/s11356-024-32372-4
    Benthic microbial fuel cell (BMFC) is the most promising type of bioelectrochemical approach for producing electrons and protons from natural organic waste. In the present work, a single-chamber BMFC was used, containing sago (Cycas revoluta) waste as the organic feed for microorganisms. The local wastewater was supplemented with heavy metal ions (Pb2+, Cd2+, Cr3+, Ni2+, Co2+, Ag+, and Cu2+) and used as an inoculation source to evaluate the performance of BMFC against the toxic metal remediations. According to the experimental results, the maximum power density obtained was 42.55 mW/m2 within 25 days of the BMFC operation. The maximum remediation efficiency of the metal ion removal from the wastewater was found to be 99.30% (Ag+). The conductive pili-type bacteria species (Acinetobacter species, Leucobacter species, Bacillus species, Proteus species. and Klebsiella pneumoniae) were found in the present study during isolation and identification processes. This study's multiple parameter optimization revealed that pH 7 and room temperature is the best condition for optimal performance. Finally, this study included the mechanism, future recommendations, and concluding remarks.
    Matched MeSH terms: Bacteria/metabolism
  5. Insight into recent advances in microalgae biogranulation in wastewater treatment
    Kabir Ahmad SF, Kanadasan G, Lee KT, Vadivelu VM
    Crit Rev Biotechnol, 2024 Dec;44(8):1594-1609.
    PMID: 38485522 DOI: 10.1080/07388551.2024.2317785
    Microalgae-based technology is widely utilized in wastewater treatment and resource recovery. However, the practical implementation of microalgae-based technology is hampered by the difficulty in separating microalgae from treated water due to the low density of microalgae. This review is designed to find the current status of the development and utilization of microalgae biogranulation technology for better and more cost-effective wastewater treatment. This review reveals that the current trend of research is geared toward developing microalgae-bacterial granules. Most previous works were focused on studying the effect of operating conditions to improve the efficiency of wastewater treatment using microalgae-bacterial granules. Limited studies have been directed toward optimizing operating conditions to induce the secretion of extracellular polymeric substances (EPSs), which promotes the development of denser microalgae granules with enhanced settling ability. Likewise, studies on the understanding of the EPS role and the interaction between microalgae cells in forming granules are scarce. Furthermore, the majority of current research has been on the cultivation of microalgae-bacteria granules, which limits their application only in wastewater treatment. Cultivation of microalgae granules without bacteria has greater potential because it does not require additional purification and can be used for border applications.
    Matched MeSH terms: Bacteria/metabolism
  6. Fulltext Bactericidal Effect of Pterostilbene Alone and in Combination with Gentamicin against Human Pathogenic Bacteria
    Lee WX, Basri DF, Ghazali AR
    Molecules, 2017 Mar 17;22(3).
    PMID: 28304328 DOI: 10.3390/molecules22030463
    The antibacterial activity of pterostilbene in combination with gentamicin against six strains of Gram-positive and Gram-negative bacteria were investigated. The minimum inhibitory concentration and minimum bactericidal concentration of pterostilbene were determined using microdilution technique whereas the synergistic antibacterial activities of pterostilbene in combination with gentamicin were assessed using checkerboard assay and time-kill kinetic study. Results of the present study showed that the combination effects of pterostilbene with gentamicin were synergistic (FIC index < 0.5) against three susceptible bacteria strains: Staphylococcus aureus ATCC 25923, Escherichia coli O157 and Pseudomonas aeruginosa 15442. However, the time-kill study showed that the interaction was indifference which did not significantly differ from the gentamicin treatment. Furthermore, time-kill study showed that the growth of the tested bacteria was completely attenuated with 2 to 8 h treatment with 0.5 × MIC of pterostilbene and gentamicin. The identified combinations could be of effective therapeutic value against bacterial infections. These findings have potential implications in delaying the development of bacterial resistance as the antibacterial effect was achieved with the lower concentrations of antibacterial agents.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology*; Anti-Bacterial Agents/chemistry; Bacteria/drug effects*; Bacteria/ultrastructure; Gram-Negative Bacteria/drug effects; Gram-Negative Bacteria/ultrastructure; Gram-Positive Bacteria/drug effects; Gram-Positive Bacteria/ultrastructure
  7. Bacteria consortia enhanced hydrocarbon degradation of waxy crude oil
    Rahman RNZRA, Latip W, Adlan NA, Sabri S, Ali MSM
    Arch Microbiol, 2022 Nov 12;204(12):701.
    PMID: 36370212 DOI: 10.1007/s00203-022-03316-8
    Waxy crude oil is a problem to the oil and gas industry because wax deposition in pipelines reduces the quality of the crude oil. Currently, the industry uses chemicals to solve the problem but it is not environmentally friendly. As an alternative, the biodegradation approach is one of the options. Previously eleven thermophilic bacteria were isolated and exhibited high ability to degrade hydrocarbon up to 70% of waxy crude oil. However, despite the successful study on these single bacteria strains, it is believed that biodegradation of paraffin wax requires more than a single species. Five consortia were developed based on the biodegradation efficiency of 11 bacterial strains. Consortium 3 showed the highest biodegradation (77.77%) with more long-chain alkane degraded throughout the incubation compared to other consortia. Enhancement of hydrocarbon degradation was observed for all consortia especially in long chain alkane (C18-C40). Consortium 3 exhibited higher alkane monooxygenase, alcohol dehydrogenase, lipase, and esterase activities. Moreover, the dominant bacteria in the consortia were determined by denaturing gradient gel electrophoresis (DGGE), which showed the domination of genera Geobacillus, Parageobacillus, and Anoxybacillus. It can be concluded that the bacterial consortia showed higher biodegradation and improved degrading more long-chain hydrocarbon compared to a single isolate.
    Matched MeSH terms: Bacteria/genetics; Bacteria/metabolism
  8. Fulltext Type II bacterial toxin-antitoxins: hypotheses, facts, and the newfound plethora of the PezAT system
    Chan WT, Garcillán-Barcia MP, Yeo CC, Espinosa M
    FEMS Microbiol Rev, 2023 Sep 05;47(5).
    PMID: 37715317 DOI: 10.1093/femsre/fuad052
    Toxin-antitoxin (TA) systems are entities found in the prokaryotic genomes, with eight reported types. Type II, the best characterized, is comprised of two genes organized as an operon. Whereas toxins impair growth, the cognate antitoxin neutralizes its activity. TAs appeared to be involved in plasmid maintenance, persistence, virulence, and defence against bacteriophages. Most Type II toxins target the bacterial translational machinery. They seem to be antecessors of Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) RNases, minimal nucleotidyltransferase domains, or CRISPR-Cas systems. A total of four TAs encoded by Streptococcus pneumoniae, RelBE, YefMYoeB, Phd-Doc, and HicAB, belong to HEPN-RNases. The fifth is represented by PezAT/Epsilon-Zeta. PezT/Zeta toxins phosphorylate the peptidoglycan precursors, thereby blocking cell wall synthesis. We explore the body of knowledge (facts) and hypotheses procured for Type II TAs and analyse the data accumulated on the PezAT family. Bioinformatics analyses showed that homologues of PezT/Zeta toxin are abundantly distributed among 14 bacterial phyla mostly in Proteobacteria (48%), Firmicutes (27%), and Actinobacteria (18%), showing the widespread distribution of this TA. The pezAT locus was found to be mainly chromosomally encoded whereas its homologue, the tripartite omega-epsilon-zeta locus, was found mostly on plasmids. We found several orphan pezT/zeta toxins, unaccompanied by a cognate antitoxin.
    Matched MeSH terms: Bacteria/genetics; Bacteria/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism
  9. Fulltext Diversity of isoprene-degrading bacteria in phyllosphere and soil communities from a high isoprene-emitting environment: a Malaysian oil palm plantation
    Carrión O, Gibson L, Elias DMO, McNamara NP, van Alen TA, Op den Camp HJM, et al.
    Microbiome, 2020 06 03;8(1):81.
    PMID: 32493439 DOI: 10.1186/s40168-020-00860-7
    BACKGROUND: Isoprene is the most abundantly produced biogenic volatile organic compound (BVOC) on Earth, with annual global emissions almost equal to those of methane. Despite its importance in atmospheric chemistry and climate, little is known about the biological degradation of isoprene in the environment. The largest source of isoprene is terrestrial plants, and oil palms, the cultivation of which is expanding rapidly, are among the highest isoprene-producing trees.

    RESULTS: DNA stable isotope probing (DNA-SIP) to study the microbial isoprene-degrading community associated with oil palm trees revealed novel genera of isoprene-utilising bacteria including Novosphingobium, Pelomonas, Rhodoblastus, Sphingomonas and Zoogloea in both oil palm soils and on leaves. Amplicon sequencing of isoA genes, which encode the α-subunit of the isoprene monooxygenase (IsoMO), a key enzyme in isoprene metabolism, confirmed that oil palm trees harbour a novel diversity of isoA sequences. In addition, metagenome-assembled genomes (MAGs) were reconstructed from oil palm soil and leaf metagenomes and putative isoprene degradation genes were identified. Analysis of unenriched metagenomes showed that isoA-containing bacteria are more abundant in soils than in the oil palm phyllosphere.

    CONCLUSION: This study greatly expands the known diversity of bacteria that can metabolise isoprene and contributes to a better understanding of the biological degradation of this important but neglected climate-active gas. Video abstract.

    Matched MeSH terms: Bacteria/classification; Bacteria/metabolism
  10. Microbial Nanotechnology for Precision Nanobiosynthesis: Innovations, Current Opportunities and Future Perspectives for Industrial Sustainability
    Khan SS, Kour D, Kaur T, Sharma A, Kumar S, Kumari S, et al.
    Curr Microbiol, 2024 Jul 01;81(8):251.
    PMID: 38954017 DOI: 10.1007/s00284-024-03772-z
    A new area of biotechnology is nanotechnology. Nanotechnology is an emerging field that aims to develope various substances with nano-dimensions that have utilization in the various sectors of pharmaceuticals, bio prospecting, human activities and biomedical applications. An essential stage in the development of nanotechnology is the creation of nanoparticles. To increase their biological uses, eco-friendly material synthesis processes are becoming increasingly important. Recent years have shown a lot of interest in nanostructured materials due to their beneficial and unique characteristics compared to their polycrystalline counterparts. The fascinating performance of nanomaterials in electronics, optics, and photonics has generated a lot of interest. An eco-friendly approach of creating nanoparticles has emerged in order to get around the drawbacks of conventional techniques. Today, a wide range of nanoparticles have been created by employing various microbes, and their potential in numerous cutting-edge technological fields have been investigated. These particles have well-defined chemical compositions, sizes, and morphologies. The green production of nanoparticles mostly uses plants and microbes. Hence, the use of microbial nanotechnology in agriculture and plant science is the main emphasis of this review. The present review highlights the methods of biological synthesis of nanoparticles available with a major focus on microbially synthesized nanoparticles, parameters and biochemistry involved. Further, it takes into account the genetic engineering and synthetic biology involved in microbial nanobiosynthesis to the construction of microbial nanofactories.
    Matched MeSH terms: Bacteria/genetics; Bacteria/metabolism
  11. Fulltext Complete Genome Sequence of Pseudomonas aeruginosa Strain YL84, a Quorum-Sensing Strain Isolated from Compost
    Chan KG, Yin WF, Lim YL
    Genome Announc, 2014;2(2).
    PMID: 24699957 DOI: 10.1128/genomeA.00246-14
    Here, we report the complete genome sequence of Pseudomonas aeruginosa strain YL84, which was isolated from compost. This strain was found to be a chitinase-producing quorum-sensing bacterium.
    Matched MeSH terms: Bacteria
  12. Fulltext Whole-genome sequences of 13 endophytic bacteria isolated from shrub willow (salix) grown in geneva, new york
    Gan HY, Gan HM, Savka MA, Triassi AJ, Wheatley MS, Smart LB, et al.
    Genome Announc, 2014;2(3).
    PMID: 24812212 DOI: 10.1128/genomeA.00288-14
    Shrub willow, Salix spp. and hybrids, is an important bioenergy crop. Here we report the whole-genome sequences and annotation of 13 endophytic bacteria from stem tissues of Salix purpurea grown in nature and from commercial cultivars and Salix viminalis × Salix miyabeana grown in bioenergy fields in Geneva, New York.
    Matched MeSH terms: Bacteria
  13. Fulltext Microbiome and Biocatalytic Bacteria in Monkey Cup (Nepenthes Pitcher) Digestive Fluid
    Chan XY, Hong KW, Yin WF, Chan KG
    Sci Rep, 2016 Jan 28;6:20016.
    PMID: 26817720 DOI: 10.1038/srep20016
    Tropical carnivorous plant, Nepenthes, locally known as "monkey cup", utilises its pitcher as a passive trap to capture insects. It then secretes enzymes into the pitcher fluid to digest the insects for nutrients acquisition. However, little is known about the microbiota and their activity in its pitcher fluid. Eighteen bacteria phyla were detected from the metagenome study in the Nepenthes pitcher fluid. Proteobacteria, Bacteroidetes and Actinobacteria are the dominant phyla in the Nepenthes pitcher fluid. We also performed culturomics approach by isolating 18 bacteria from the Nepenthes pitcher fluid. Most of the bacterial isolates possess chitinolytic, proteolytic, amylolytic, and cellulolytic and xylanolytic activities. Fifteen putative chitinase genes were identified from the whole genome analysis on the genomes of the 18 bacteria isolated from Nepenthes pitcher fluid and expressed for chitinase assay. Of these, six clones possessed chitinase activity. In conclusion, our metagenome result shows that the Nepenthes pitcher fluid contains vast bacterial diversity and the culturomic studies confirmed the presence of biocatalytic bacteria within the Nepenthes pitcher juice which may act in symbiosis for the turn over of insects trapped in the Nepenthes pitcher fluid.
    Matched MeSH terms: Bacteria/classification; Bacteria/genetics; Bacteria/metabolism; Genome, Bacterial
  14. Fulltext Insights on Quorum-Quenching Properties of Lysinibacillus fusiformis Strain RB21, a Malaysian Municipal Solid-Waste Landfill Soil Isolate, via Complete Genome Sequence Analysis
    Yong D, Ee R, Lim YL, Chang CY, Yin WF, Chan KG
    Genome Announc, 2015;3(3).
    PMID: 25953192 DOI: 10.1128/genomeA.00409-15
    Lysinibacillus fusiformis strain RB21 is a quorum-quenching bacterium that is able to degrade quorum-sensing signaling molecules. Here, we present the first complete genome sequence of L. fusiformis strain RB21. The finished genome is 4.8 Mbp in size, and the quorum-quenching gene was identified.
    Matched MeSH terms: Bacteria
  15. Distinctive phyllosphere bacterial communities in tropical trees
    Kim M, Singh D, Lai-Hoe A, Go R, Abdul Rahim R, Ainuddin AN, et al.
    Microb Ecol, 2012 Apr;63(3):674-81.
    PMID: 21990015 DOI: 10.1007/s00248-011-9953-1
    Recent work has suggested that in temperate and subtropical trees, leaf surface bacterial communities are distinctive to each individual tree species and dominated by Alpha- and Gammaproteobacteria. In order to understand how general this pattern is, we studied the phyllosphere bacterial community on leaves of six species of tropical trees at a rainforest arboretum in Malaysia. This represents the first detailed study of 'true' tropical lowland tree phyllosphere communities. Leaf surface DNA was extracted and pyrosequenced targeting the V1-V3 region of 16S rRNA gene. As was previously found in temperate and subtropical trees, each tree species had a distinctive bacterial community on its leaves, clustering separately from other tree species in an ordination analysis. Bacterial communities in the phyllosphere were unique to plant leaves in that very few operational taxonomic units (0.5%) co-occurred in the surrounding soil environment. A novel and distinctive aspect of tropical phyllosphere communities is that Acidobacteria were one of the most abundant phyla across all samples (on average, 17%), a pattern not previously recognized. Sequences belonging to Acidobacteria were classified into subgroups 1-6 among known 24 subdivisions, and subgroup 1 (84%) was the most abundant group, followed by subgroup 3 (15%). The high abundance of Acidobacteria on leaves of tropical trees indicates that there is a strong relationship between host plants and Acidobacteria in tropical rain forest, which needs to be investigated further. The similarity of phyllosphere bacterial communities amongst the tree species sampled shows a significant tendency to follow host plant phylogeny, with more similar communities on more closely related hosts.
    Matched MeSH terms: Bacteria/classification; Bacteria/genetics; Bacteria/isolation & purification*
  16. Impact of Land-use Change on Vertical Soil Bacterial Communities in Sabah
    Tin HS, Palaniveloo K, Anilik J, Vickneswaran M, Tashiro Y, Vairappan CS, et al.
    Microb Ecol, 2018 Feb;75(2):459-467.
    PMID: 28779295 DOI: 10.1007/s00248-017-1043-6
    Decline in forest productivity due to forest conversion is defining the Bornean landscape. Responses of bacterial communities due to land-use changes are vital and could define our understanding of ecosystem functions. This study reports the changes in bacterial community structure in organic soil (0-5 cm; O-Horizon) and organic-mineral soil (5-15 cm; A-Horizon) across Maliau Basin Conservation Area old growth forest (MBOG), Fragment E logged forest (FELF) located in Kalabakan Forest Reserve to Benta Wawasan oil palm plantation (BWOP) using two-step PCR amplicon analysis of bacteria DNA on Illumina Miseq next generation sequencing. A total of 30 soil samples yielded 893,752-OTU reads at ≥97% similarity from 5,446,512 good quality sequences. Soil from BWOP plantation showed highest unshared OTUs for organic (49.2%) and organic-mineral (50.9%) soil. MBOG soil showed a drop in unshared OTUs between organic (48.6%) and organic-mineral (33.9%). At phylum level, Proteobacteria dominated MBOG but shifted to Actinobacteria in logged and plantation soil. Present findings also indicated that only FELF exhibited change in bacterial communities along the soil depth, moving from the organic to the organic-mineral layer. Both layers of BWOP plantation soils deviated from other forests' soil in β-diversity analysis. To our knowledge, this is the first report on transitions of bacterial community structures with different soil horizons in the tropical rainforest including Borneo, Sabah. Borneo tropical soils form a large reservoir for soil bacteria and future exploration is needed for fully understanding the diversity structure and their bacterial functional properties.
    Matched MeSH terms: Bacteria/classification; Bacteria/genetics; Bacteria/isolation & purification*
  17. Fulltext Draft Genome Sequences of Longimonas halophila KCTC 42399 and Longibacter salinarum KCTC 52045
    Liew KJ, Teo SC, Shamsir MS, Goh KM
    Microbiol Resour Announc, 2019 Nov 14;8(46).
    PMID: 31727717 DOI: 10.1128/MRA.01238-19
    Longimonas halophila and Longibacter salinarum are type strains of underexplored genera affiliated with Salisaetaceae Herein, we report the draft genome sequences of two strains of these bacteria, L. halophila KCTC 42399 and L. salinarum KCTC 52045, with the intent of broadening knowledge of this family. Genome annotation and gene mining revealed that both bacteria exhibit amylolytic abilities.
    Matched MeSH terms: Bacteria
  18. Complete genome of Pandoraea pnomenusa RB-38, an oxalotrophic bacterium isolated from municipal solid waste landfill site
    Lim YL, Ee R, Yong D, Tee KK, Yin WF, Chan KG
    J Biotechnol, 2015 Nov 20;214:83-4.
    PMID: 26393955 DOI: 10.1016/j.jbiotec.2015.09.018
    Pandoraea pnomenusa RB-38 is a bacterium isolated from a former sanitary landfill site. Here, we present the complete genome of P. pnomenusa RB38 in which an oxalate utilization pathway was identified. The genome analysis suggested the potential of this strain as an effective biocontrol agent against oxalate-producing phytopathogens.
    Matched MeSH terms: Bacteria
  19. Fulltext Draft Genome Sequence of Halophilic Hahella sp. Strain CCB-MM4, Isolated from Matang Mangrove Forest in Perak, Malaysia
    Sam KK, Lau NS, Furusawa G, Amirul AA
    Genome Announc, 2017 Oct 19;5(42).
    PMID: 29051257 DOI: 10.1128/genomeA.01147-17
    Hahella sp. strain CCB-MM4 is a halophilic bacterium isolated from estuarine mangrove sediment. The genome sequence of Hahella sp. CCB-MM4 provides insights into exopolysaccharide biosynthesis and the lifestyle of the bacterium thriving in a saline mangrove environment.
    Matched MeSH terms: Bacteria; Gammaproteobacteria
  20. Antimicrobial, antiadhesive, and antibiofilm actions of rhamnolipids on ESKAPE pathogens
    Firdose A, Chong NHH, Ramli R, Aqma WS
    Lett Appl Microbiol, 2023 Feb 16;76(2).
    PMID: 36702549 DOI: 10.1093/lambio/ovad013
    The aim of this study was to test the antimicrobial, antiadhesive, and antibiofilm activities of a rhamnolipid extracted from Pseudomonas aeruginosa UKMP14T previously isolated from oil-contaminated soil in Malaysia against ESKAPE (i.e. multidrug resistant) pathogens. Zones of inhibition in an agar well diffusion assay were observed at 50 µg mL-1 concentrations of rhamnolipid for all the ESKAPE bacteria. The MIC and MBC values ranged between 7.81-62.5 µg mL-1 and 31.25-1000 µg mL-1, respectively. Percent killing was recorded to be >90% except for Klebsiella pneumoniae (86.84%). Furthermore, antiadhesion studies showed that there was 76% hindrance in attachment of Enterococcus faecium and 91% in Acinetobacter baumannii at 4 × MIC. The highest inhibition in adhesion was found at 4 × MIC, which was 46% for Ac. baumannii and 62% for Enterococcus faecium. Finally, the antibiofilm capability of the rhamnolipid was determined, which ranged between 25%-76% in Ac. baumannii and 35%-88% in Enterococcus faecium. To the best of our knowledge, this is the first study to include research on antimicrobial, antiadhesive and antibiofilm activities of rhamnolipid from the local isolate Ps. aeruginosa UKMP14T against ESKAPE bacteria. Obtained results suggest that this rhamnolipid can be exploited commercially for the production of novel antibiotics.
    Matched MeSH terms: Bacteria
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