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Homozygous variant of UGT1A1 gene mutation and severe neonatal hyperbilirubinemia

Boo NY, Wong FL, Wang MK, Othman A

Pediatr Int, 2009 Aug;51(4):488-93.
PMID: 19674361 DOI: 10.1111/j.1442-200X.2008.02798.x

The aim of the present study was to compare, in a case-control study, the prevalence of nucleotide 211 guanine to adenine (G-->A) mutation of uridine diphosphoglucuronosyl transferase (UGT1A1) gene in Malaysian Chinese newborns with and without severe hyperbilirubinemia (total serum bilirubin >250 micromol/L during first 48 h of life or > or =300 micromol/L thereafter), and to determine whether this mutation was a significant risk factor associated with severe hyperbilirubinemia.
In vitro expression of erythropoietin by transfected human mesenchymal stromal cells

Mok PL, Cheong SK, Leong CF, Othman A

Cytotherapy, 2008;10(2):116-24.
PMID: 18368590 DOI: 10.1080/14653240701816996

Mesenchymal stromal cells (MSC) are pluripotent progenitor cells that can be found in human bone marrow (BM). These cells have low immunogenicity and could suppress alloreactive T-cell responses. In the current study, MSC were tested for their capacity to carry and deliver the erythropoietin (EPO) gene in vitro.
Primary thrombocythaemia presenting as postpartum haemorrhage: a case report

Rashid Z, Hamidah NH, Othman A, Cheong SK, Fairuz AK, Adeeb N

J Obstet Gynaecol (Tokyo 1995), 1995 Jun;21(3):221-5.
PMID: 8590357

A young primigravida presented with postpartum haemorrhage with no apparent cause following a low forceps delivery. She was extremely pale with gross hepatosplenomegaly. Hysterectomy was performed following three episodes of disseminated intravascular coagulation. Investigations revealed an extremely high platelet count with poor aggregatory function. A diagnosis of primary thrombocythaemia was made.
Fulltext Barriers to unmet needs among mothers of children with disabilities in Kelantan, Malaysia: A qualitative study

Sukeri S, Bakar RS, Othman A, Ibrahim MI

J Taibah Univ Med Sci, 2017 Oct;12(5):424-429.
PMID: 31435274 DOI: 10.1016/j.jtumed.2017.05.002

Objectives: The present study aimed to determine the barriers that deter mothers of children with disabilities from attaining their unmet needs.

Methods: In-depth interviews of 12 mothers of children with disabilities were conducted from November 2014 to January 2015 in Kelantan, Malaysia. The mothers were recruited by purposive sampling. Thematic analysis was used for identifying, analysing and reporting the data.

Results: Barriers to the unmet needs among mothers of children with disabilities were found to stem from the mothers' expectation and further propagated by lack of support, the role of healthcare professionals in providing care, inappropriate policies and shortage of resources required for survival and maintaining care.

Conclusions: Identification of the barriers to the attainment of unmet needs among mothers of disabled children is crucial for resolving the issue of unmet needs. Deeper understanding of these barriers may facilitate positive actions toward addressing the needs of these mothers and to alleviate the stress on mothers of disabled children. A concerted effort to coordinate services across all disciplines is required to dismantle these barriers by improving the provision of health care delivery and evaluation of welfare policies and services.
Fulltext Association between secondhand smoke exposure at home and cognitive performance among rural primary school children in Malaysia

Ellis-Suriani Z, Norsa'adah B, Othman A, Siti-Azrin AH

Tob Induc Dis, 2021;19:27.
PMID: 33867904 DOI: 10.18332/tid/133638

INTRODUCTION: Secondhand smoke (SHS) exposure is linked to a series of serious health problems. Children may be particularly vulnerable to the effects of SHS exposure at home. This study aimed to determine the association between SHS exposure at home and cognitive performance in school children.
METHODS: A multistage sampling was performed across rural primary schools in Kuala Krai, Kelantan, Malaysia. Data were collected using self-administered questionnaires and the children aged 10-11 years (n=312) were subjected to cognitive tests including digit span, letter-number sequencing, coding, and symbol search. Cognitive performance was tested using subscales derived from the Wechsler Intelligence Scale for Children.
RESULTS: The prevalence of SHS exposure at home was 55.8%, where 11.9% of children lived with one smoker, while 43.9% of children lived with ≥2 smokers. There was a significant difference in the mean score of the combined cognitive tests between SHS-exposed and non-exposed children after adjustment for sex, parental educational level, family income and academic performance [Pillai's Trace=0.084, F statistic (df)=6.803 (4302), p<0.001].
CONCLUSIONS: More than half of the primary school children in rural Kuala Krai were exposed to SHS from at least one smoker at home. There was a significant association between SHS exposure at home and cognitive performance.
Glucose-6-phosphate dehydrogenase (G6PD)-deficient infants: Enzyme activity and gene variants as risk factors for phototherapy in the first week of life

Wong FL, Ithnin A, Othman A, Cheah FC

J Paediatr Child Health, 2017 Jul;53(7):705-710.
PMID: 28376293 DOI: 10.1111/jpc.13509

AIM: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a recognised cause of severe neonatal hyperbilirubinaemia, and identifying which infants are at risk could optimise care and resources. In this study, we determined if G6PD enzyme activity (EA) and certain gene variants were associated with neonatal hyperbilirubinaemia requiring phototherapy during the first week after birth.
METHODS: Newborn infants with G6PD deficiency and a group with normal results obtained by the fluorescent spot test were selected for analyses of G6PD EA and the 10 commonly encountered G6PD mutations in this region, relating these with whether the infants required phototherapy before discharge from the hospital in the first week.
RESULTS: A total of 222 infants with mean gestation and birth weight of 38.3 ± 1.8 weeks and 3.02 ± 0.48 kg, respectively, were enrolled. Of these, n = 121 were deficient with EA ≤6.76 U/g Hb, and approximately half (43%) received phototherapy in the first week after birth. The mean EA level was 3.7 U/g Hb. The EA had good accuracy in predicting phototherapy use, with area under the receiver-operating-characteristic curve of 0.81 ± 0.05. Infants on phototherapy more commonly displayed World Health Organization Class II mutations (<10% residual EA). Logistic regression analysis showed that deficiency in EA and mutation at c.1388G>A (adjusted odds ratio, 1.5 and 5.7; 95% confidence interval: 1.31-1.76 and 1.30-25.0, respectively) were independent risk factors for phototherapy.
CONCLUSION: Low G6PD EA (<6.76 U/g Hb) and the G6PD gene variant, c.1388G>A, are risk factors for the need of phototherapy in newborn infants during the first week after birth.
Study site: Pusat Perubatan Universiti Kebangsaan Malaysia (PPUKM), Kuala Lumpur, Malaysia
Fulltext The Validity and Reliability of the Malay Version of the Cyberbullying Scale among Secondary School Adolescents in Malaysia

Saman ZM, Siti-Azrin AH, Othman A, Kueh YC

Int J Environ Res Public Health, 2021 11 06;18(21).
PMID: 34770183 DOI: 10.3390/ijerph182111669

The effect of cyberbullying among adolescents in Malaysia is not much studied. The Cyberbullying Scale (CBS) has been validated to be used among English speaking adolescents to measure cyberbullying but not in Malay language. Therefore, its validity should be established before use in the Malaysian context. Thus, the study aimed to evaluate the validity and reliability of the Malay version of the CBS (CBS-M) among secondary school students. The study was cross-sectional and involved a self-administered questionnaire with 16 items from CBS-M, and 21 items from the Depression, Anxiety, and Stress Scale (DASS-21). Participants were recruited using a multi-stage sampling method. The validity of the CBS-M was tested in two phases, namely, exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). Spearman's correlation was used to examine the strength of the relationship between the CBS and subscales from DASS-21 to further support the validity of CBS-M. A total of 401 respondents from Muar, Johor, participated. The mean age was 14.6 years (SD = 1.25). EFA results indicated a one-factor model of CBS-M with a total variance extracted of 33.9%. Internal consistency measured by Cronbach's alpha reached 0.87. The model was then tested using CFA. The initial model did not fit the data well. Thus, several model re-specifications were conducted on the initial model. The final measurement model of CBS-M fit the data well with acceptable fit indices (CFI = 0.946, TLI = 0.932, SRMR = 0.055, RMSEA = 0.049). The composite reliability for CBS-M was satisfactory with a value of 0.832. The CBS-M questionnaire is a valid and reliable tool for measuring cyberbullying among young adolescents in Malaysia.
Psychological markers underlying murder weapon profile: a quantitative study

Kamaluddin MR, Othman A, Ismail KH, Mat Saat GA

Malays J Pathol, 2017 Dec;39(3):217-226.
PMID: 29279583 MyJurnal

The horrific nature of murder using different types of weapons has been an important focal point of many criminological studies. Weapons that are used in murders seem to play dominant roles in murder investigations as they may provide information leading to arrest. The established factors for weapon usage include environmental context, demography and availability of weapons. However, there is insufficient research attention on the psychological functioning of murderers for particular weapon usage. In light of this, the current study seeks to narrow this gap of information by identifying the influences of psychological traits on weapon usage among a sample of male murderers. The present cross-sectional study was conducted among 71 male murderers incarcerated in 11 prisons within Peninsular Malaysia. The selection of the sample was based on predetermined selection criteria using a purposive sampling method. A guided self-administered questionnaire comprising sociodemography variables and four Malay validated psychometric instruments: Zuckerman-Kuhlman Personality Questionnaire-40-Cross-Culture, Self-control Scale, "How I Think" Questionnaire and Aggression Questionnaire; was used. Independent sample t-test was performed to establish the mean score differences of psychological traits between the murderers who used single and multiple weapons while Kruskal-Wallis tests were carried out to ascertain the differences between the specific types of weapons used among the murderers. Following this, one-way ANOVA was carried out to ascertain the psychological trait differences among the murderers according to the different sources of weapon. Results indicated specific psychological traits influenced the number(s), source(s) and type(s) of weapon used in committing murder. The findings have implications for the psychological profiling of unknown murderers within the Malaysian context.
MXenes and MXene-based materials for removal of pharmaceutical compounds from wastewater: Critical review

Tawalbeh M, Mohammed S, Al-Othman A, Yusuf M, Mofijur M, Kamyab H

Environ Res, 2023 Jul 01;228:115919.
PMID: 37072081 DOI: 10.1016/j.envres.2023.115919

The rapid increase in the global population and its ever-rising standards of living are imposing a huge burden on global resources. Apart from the rising energy needs, the demand for freshwater is correspondingly increasing. A population of around 3.8 billion people will face water scarcity by 2030, as per the reports of the World Water Council. This may be due to global climate change and the deficiency in the treatment of wastewater. Conventional wastewater treatment technologies fail to completely remove several emerging contaminants, especially those containing pharmaceutical compounds. Hence, leading to an increase in the concentration of harmful chemicals in the human food chain and the proliferation of several diseases. MXenes are transition metal carbide/nitride ceramics that primarily structure the leading 2D material group. MXenes act as novel nanomaterials for wastewater treatment due to their high surface area, excellent adsorption properties, and unique physicochemical properties, such as high electrical conductivity and hydrophilicity. MXenes are highly hydrophilic and covered with active functional groups (i.e., hydroxyl, oxygen, fluorine, etc.), which makes them efficient adsorbents for a wide range of species and promising candidates for environmental remediation and water treatment. This work concludes that the scaling up process of MXene-based materials for water treatment is currently of high cost. The up-to-date applications are still limited because MXenes are currently produced mainly in the laboratory with limited yield. It is recommended to direct research efforts towards lower synthesis cost procedures coupled with the use of more environmentally friendly materials to avoid secondary contamination.
Sequence analysis and gene expression of putative exo- and endo-glucanases from oil palm (Elaeis guineensis) during fungal infection

Yeoh KA, Othman A, Meon S, Abdullah F, Ho CL

J Plant Physiol, 2012 Oct 15;169(15):1565-70.
PMID: 22854183 DOI: 10.1016/j.jplph.2012.07.006

Glucanases are enzymes that hydrolyze a variety β-d-glucosidic linkages. Plant β-1,3-glucanases are able to degrade fungal cell walls; and promote the release of cell-wall derived fungal elicitors. In this study, three full-length cDNA sequences encoding oil palm (Elaeis guineensis) glucanases were analyzed. Sequence analyses of the cDNA sequences suggested that EgGlc1-1 is a putative β-d-glucan exohydolase belonging to glycosyl hydrolase (GH) family 3 while EgGlc5-1 and EgGlc5-2 are putative glucan endo-1,3-β-glucosidases belonging to GH family 17. The transcript abundance of these genes in the roots and leaves of oil palm seedlings treated with Ganoderma boninense and Trichoderma harzianum was profiled to investigate the involvement of these glucanases in oil palm during fungal infection. The gene expression of EgGlc1-1 in the root of oil palm seedlings was increased by T. harzianum but suppressed by G. boninense; while the gene expression of both EgGlc5-1 and EgGlc5-2 in the roots of oil palm seedlings was suppressed by G. boninense or/and T. harzianum.
Cystic Meningioma Masquerading as a Metastatic Tumor: A Case Report

Ramanathan N, Kamaruddin KA, Othman A, Mustafa F, Awang MS

Malays J Med Sci, 2016 May;23(3):92-4.
PMID: 27418876 MyJurnal

Cystic meningioma is a rare form of intracranial meningioma. Meningiomas are typically solid tumors but may rarely have cystic components. The diagnosis of cystic meningioma is clinically challenging as the finding of multiple intra-axial tumors, including metastatic tumors, is relatively common. We report a case of cystic meningioma initially diagnosed as a metastatic tumor from a recurrence of acute lymphoid leukemia. However, postoperative histopathological examination demonstrated an atypical meningioma.
Fulltext Wernicke's Encephalopthy Associated with Hyperemesis Gravidarum - A Case Report

Sulaiman W, Othman A, Mohamad M, Salleh HR, Mushahar L

Malays J Med Sci, 2002 Jul;9(2):43-6.
PMID: 22844223 MyJurnal

Two cases of Wernicke's encephalopathy due to hyperemesis gravidarum are described. The first patient presented with bilateral papilloedema, altered sensorium and the second with bilateral retinal haemorrhages, ophthalmoplegia and nystagmus. Both patients were diagnosed with Wernicke's encephalopathy on clinical ground since there were no laboratory facilities to measure red cell transketolase and thiamine pyrophosphate levels. This is a rare but treatable complication of hyperemesis gravidarum (HG) and due to lack of diagnostic tools, there is often diagnostic uncertainty, delay in commencing appropriate treatment, as well as irreversible damage to the upper brain stem and death.
Unravelling the temporal association between lameness and body condition score in dairy cattle using a multistate modelling approach

Lim PY, Huxley JN, Willshire JA, Green MJ, Othman AR, Kaler J

Prev Vet Med, 2015 Mar 1;118(4):370-7.
PMID: 25579605 DOI: 10.1016/j.prevetmed.2014.12.015

Recent studies have reported associations between lameness and body condition score (BCS) in dairy cattle, however the impact of change in the dynamics of BCS on both lameness occurrence and recovery is currently unknown. The aim of this longitudinal study was to investigate the effect of change in BCS on the transitions from the non-lame to lame, and lame to non-lame states. A total of 731 cows with 6889 observations from 4 UK herds were included in the study. Mobility score (MS) and body condition score (BCS) were recorded every 13-15 days from July 2010 until December 2011. A multilevel multistate discrete time event history model was built to investigate the transition of lameness over time. There were 1042 non-lame episodes and 593 lame episodes of which 50% (519/1042) of the non-lame episodes transitioned to the lame state and 81% (483/593) of the lame episodes ended with a transition to the non-lame state. Cows with a lower BCS at calving (BCS Group 1 (1.00-1.75) and Group 2 (2.00-2.25)) had a higher probability of transition from non-lame to lame and a lower probability of transition from lame to non-lame compared to cows with BCS 2.50-2.75, i.e. they were more likely to become lame and if lame, they were less likely to recover. Similarly, cows who suffered a greater decrease in BCS (compared to their BCS at calving) had a higher probability of becoming lame and a lower probability of recovering in the next 15 days. An increase in BCS from calving was associated with the converse effect, i.e. a lower probability of cows moving from the non-lame to the lame state and higher probability of transition from lame to non-lame. Days in lactation, quarters of calving and parity were associated with both lame and non-lame transitions and there was evidence of heterogeneity among cows in lameness occurrence and recovery. This study suggests loss of BCS and increase of BCS could influence the risk of becoming lame and the chance of recovery from lameness. Regular monitoring and maintenance of BCS on farms could be a key tool for reducing lameness. Further work is urgently needed in this area to allow a better understanding of the underlying mechanisms behind these relationships.
Fulltext Psychological traits underlying different killing methods among Malaysian male murderers

Kamaluddin M, Shariff NS, Nurfarliza S, Othman A, Ismail KH, Mat Saat GA

Malays J Pathol, 2014 Apr;36(1):41-50.
PMID: 24763234 MyJurnal

Murder is the most notorious crime that violates religious, social and cultural norms. Examining the types and number of different killing methods that used are pivotal in a murder case. However, the psychological traits underlying specific and multiple killing methods are still understudied. The present study attempts to fill this gap in knowledge by identifying the underlying psychological traits of different killing methods among Malaysian murderers. The study adapted an observational cross-sectional methodology using a guided self-administered questionnaire for data collection. The sampling frame consisted of 71 Malaysian male murderers from 11 Malaysian prisons who were selected using purposive sampling method. The participants were also asked to provide the types and number of different killing methods used to kill their respective victims. An independent sample t-test was performed to establish the mean score difference of psychological traits between the murderers who used single and multiple types of killing methods. Kruskal-Wallis tests were carried out to ascertain the psychological trait differences between specific types of killing methods. The results suggest that specific psychological traits underlie the type and number of different killing methods used during murder. The majority (88.7%) of murderers used a single method of killing. Multiple methods of killing was evident in 'premeditated' murder compared to 'passion' murder, and revenge was a common motive. Examples of multiple methods are combinations of stabbing and strangulation or slashing and physical force. An exception was premeditated murder committed with shooting, when it was usually a single method, attributed to the high lethality of firearms. Shooting was also notable when the motive was financial gain or related to drug dealing. Murderers who used multiple killing methods were more aggressive and sadistic than those who used a single killing method. Those who used multiple methods or slashing also displayed a higher level of minimisation traits. Despite its limitations, this study has provided some light on the underlying psychological traits of different killing methods which is useful in the field of criminology.
Fulltext Increased basal oxidation of peroxiredoxin 2 and limited peroxiredoxin recycling in glucose-6-phosphate dehydrogenase-deficient erythrocytes from newborn infants

Cheah FC, Peskin AV, Wong FL, Ithnin A, Othman A, Winterbourn CC

FASEB J, 2014 Jul;28(7):3205-10.
PMID: 24636884 DOI: 10.1096/fj.14-250050

Erythrocytes require glucose-6-phosphate dehydrogenase (G6PD) to generate NADPH and protect themselves against hemolytic anemia induced by oxidative stress. Peroxiredoxin 2 (Prx2) is a major antioxidant enzyme that requires NADPH to recycle its oxidized (disulfide-bonded) form. Our aims were to determine whether Prx2 is more highly oxidized in G6PD-deficient erythrocytes and whether these cells are able to recycle oxidized Prx2 after oxidant challenge. Blood was obtained from 61 Malaysian neonates with G6PD deficiency (average 33% normal activity) and 86 controls. Prx2 redox state was analyzed by Western blotting under nonreducing conditions. Prx2 in freshly isolated blood was predominantly reduced in both groups, but the median level of oxidation was significantly higher (8 vs 3%) and the range greater for the G6PD-deficient population. When treated with reagent H2O2, the G6PD-deficient erythrocytes were severely compromised in their ability to recycle oxidized Prx2, with only 27 or 4% reduction after 1 h treatment with 0.1 or 1 mM H2O2 respectively, compared with >97% reduction in control erythrocytes. The accumulation of oxidized Prx2 in oxidatively stressed erythrocytes with common G6PD variants suggests that impaired antioxidant activity of Prx2 could contribute to the hemolysis and other complications associated with the condition.-Cheah, F.-C., Peskin, A. V., Wong, F.-L., Ithnin, A., Othman, A., Winterbourn, C. C. Increased basal oxidation of peroxiredoxin 2 and limited peroxiredoxin recycling in glucose-6-phosphate dehydrogenase deficient erythrocytes from newborn infants.
Fulltext Efavirenz does not cause false-positive urine cannabis test in HIV-infected patients on Highly Active Anti-Retroviral Therapy

Koh KC, Lee WY, Eh ZW, Ismail NJ, Tee PS, Othman A, et al.

Med J Malaysia, 2013 Jun;68(3):249-52.
PMID: 23749016

Efavirenz is a non-nucleoside reverse transcriptase inhibitor used in combination with other drugs for the treatment of patients with HIV infection. Efavirenz has been reported to cause a positive urine cannabis test reaction which may create problems between HIV-infected patients on Efavirenz and law enforcement agencies. Doctors are at loss whether to issue documents certifying the potential false positive urine cannabis test with Efavirenz to patients. We investigated if the urine of HIV-infected patients on Efavirenz caused a positive urine cannabis test using the AxSYM Cannabinoids Assay®. Urine samples from 51 eligible patients on Efavirenz were tested for cannabis. All tested negative except for one who had used cannabis the day before. Efavirenz does not cause false positive urine cannabis test with the AxSYM Cannabinoids Assay®. Certification documents from doctors are therefore unnecessary.
Piloting a psycho-education program for parents of pediatric cancer patients in Malaysia

Othman A, Blunden S, Mohamad N, Mohd Hussin ZA, Jamil Osman Z

Psychooncology, 2010 Mar;19(3):326-31.
PMID: 19462470 DOI: 10.1002/pon.1584

To evaluate a psycho-educational program (PeP) for parents of children with cancer (PoCwC) in Malaysia.
Development of an inhibitive enzyme assay for copper

Shukor MY, Bakar NA, Othman AR, Yunus I, Shamaan NA, Syed MA

J Environ Biol, 2009 Jan;30(1):39-44.
PMID: 20112861

In this work the development of an inhibitive assay for copper using the molybdenum-reducing enzyme assay is presented. The enzyme is assayed using 12-molybdophosphoric acid at pH 5.0 as an electron acceptor substrate and NADH as the electron donor substrate. The enzyme converts the yellowish solution into a deep blue solution. The assay is based on the ability of copper to inhibit the molybdenum-reducing enzyme from the molybdate-reducing Serratia sp. Strain DRY5. Other heavy metals tested did not inhibit the enzyme at 10 mg l(-1). The best model with high regression coefficient to measure copper inhibition is one-phase binding. The calculated IC50 (concentration causing 50% inhibition) is 0.099 mg l(-1) and the regression coefficient is 0.98. The comparative LC50, EC50 and IC50 data for copper in different toxicity tests show that the IC50 value for copper in this study is lower than those for immobilized urease, bromelain, Rainbow trout, R. meliloti, Baker's Yeast dehydrogenase activity Spirillum volutans, P. fluorescens, Aeromonas hydrophilia and synthetic activated sludge assays. However the IC50 value is higher than those for Ulva pertusa and papain assays, but within the reported range for Daphnia magna and Microtox assays.
Fulltext Intracellular Delivery of Proteins via Fusion Peptides in Intact Plants

Ng KK, Motoda Y, Watanabe S, Sofiman Othman A, Kigawa T, Kodama Y, et al.

PLoS One, 2016;11(4):e0154081.
PMID: 27100681 DOI: 10.1371/journal.pone.0154081

In current plant biotechnology, the introduction of exogenous DNA encoding desired traits is the most common approach used to modify plants. However, general plant transformation methods can cause random integration of exogenous DNA into the plant genome. To avoid these events, alternative methods, such as a direct protein delivery system, are needed to modify the plant. Although there have been reports of the delivery of proteins into cultured plant cells, there are currently no methods for the direct delivery of proteins into intact plants, owing to their hierarchical structures. Here, we demonstrate the efficient fusion-peptide-based delivery of proteins into intact Arabidopsis thaliana. Bovine serum albumin (BSA, 66 kDa) was selected as a model protein to optimize conditions for delivery into the cytosol. The general applicability of our method to large protein cargo was also demonstrated by the delivery of alcohol dehydrogenase (ADH, 150 kDa) into the cytosol. The compatibility of the fusion peptide system with the delivery of proteins to specific cellular organelles was also demonstrated using the fluorescent protein Citrine (27 kDa) conjugated to either a nuclear localization signal (NLS) or a peroxisomal targeting signal (PTS). In conclusion, our designed fusion peptide system can deliver proteins with a wide range of molecular weights (27 to 150 kDa) into the cells of intact A. thaliana without interfering with the organelle-targeting peptide conjugated to the protein. We expect that this efficient protein delivery system will be a powerful tool in plant biotechnology.
Fulltext Evaluation of Glucose-6-Phosphate Dehydrogenase stability in stored blood samples

Jalil N, Azma RZ, Mohamed E, Ithnin A, Alauddin H, Baya SN, et al.

EXCLI J, 2016;15:155-62.
PMID: 27103895 DOI: 10.17179/excli2015-604

Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the commonest cause of neonatal jaundice in Malaysia. Recently, OSMMR2000-D G6PD Assay Kit has been introduced to quantitate the level of G6PD activity in newborns delivered in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). As duration of sample storage prior to analysis is one of the matters of concern, this study was conducted to identify the stability of G6PD enzyme during storage. A total of 188 cord blood samples from normal term newborns delivered at UKMMC were selected for this study. The cord bloods samples were collected in ethylene-diamine-tetra-acetic acid (EDTA) tubes and refrigerated at 2-8 °C. In addition, 32 out of 188 cord blood samples were spotted on chromatography paper, air-dried and stored at room temperature. G6PD enzyme activities were measured daily for 7 days using the OSMMR2000-D G6PD Assay Kit on both the EDTA blood and dried blood samples. The mean value for G6PD activity was compared between days of analysis using Student Paired T-Test. In this study, 172 out of 188 cord blood samples showed normal enzyme levels while 16 had levels corresponding to severe enzyme deficiency. The daily mean G6PD activity for EDTA blood samples of newborns with normal G6PD activity showed a significant drop on the fourth day of storage (p < 0.005) while for samples with severely deficient G6PD activity, significant drop was seen on third day of storage (p = 0.002). Analysis of dried cord blood showed a significant reduction in enzyme activity as early as the second day of storage (p = 0.001). It was also noted that mean G6PD activity for spotted blood samples were lower compared to those in EDTA tubes for all days (p = 0.001). Thus, EDTA blood samples stored at 2-8 °C appeared to have better stability in terms of their G6PD enzyme level as compared to dried blood samples on filter paper, giving a storage time of up to 3 days.

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