Displaying publications 201 - 220 of 2191 in total

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  1. Soon SH, Chai Kim Kai
    Med J Malaya, 1969 Dec;24(2):145-6.
    PMID: 4244140
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology*
  2. Imchen M, Kumavath R
    Environ Pollut, 2021 Jan 01;268(Pt A):115795.
    PMID: 33068846 DOI: 10.1016/j.envpol.2020.115795
    Mangrove sediments are prone to anthropogenic activities that could enrich antibiotics resistance genes (ARGs). The emergence and dissemination of ARGs are of serious concern to public health worldwide. Therefore, a comprehensive resistome analysis of global mangrove sediment is of paramount importance. In this study, we have implemented a deep machine learning approach to analyze the resistome of mangrove sediments from Brazil, China, Saudi Arabia, India, and Malaysia. Geography (RANOSIM = 39.26%; p  0.05). Several genes including multidrug efflux pumps were significantly (p 
    Matched MeSH terms: Anti-Bacterial Agents*
  3. Lim SW, Lee WS, Mani SA, Kadir K
    Eur Arch Paediatr Dent, 2020 Feb;21(1):145-154.
    PMID: 31332632 DOI: 10.1007/s40368-019-00466-0
    PURPOSE: To investigate the outpatient and inpatient management of odontogenic infections among paediatric patients attending a university hospital.

    METHODS: A retrospective study (2013-2015) was carried out which involved retrieving relevant data from past records (manual/electronic) of paediatric patients (under 18-years-old) who presented with odontogenic infections to the Paediatric Dentistry and Oral and Maxillofacial clinic. Data collected was organised using descriptive statistics with SPSS version 12.0.1.

    RESULTS: A total of 153 patients were identified, of which 83.7% were managed as outpatients. Odontogenic infections were more common in females (52.9%) and preschool children (58.2%). The most cases were seen in 2014 and maximum number of cases per month was 12. Common presentations were pain (62.1%), intraoral swelling (37.9%) and spontaneous pus discharge from the tooth and/or surrounding tissues (67.3%) with higher involvement of primary right molars. Dental panoramic tomograph was the most common radiographic investigation done. Outpatients were commonly managed chairside with pulpal opening (46.1%) at the paediatric dental clinic and 7% underwent extraction under general anaesthesia in day-care setting. Inpatients were admitted for 3 days on average and most commonly definitive care was extraction under local/general anaesthesia (68%). There were 22.7% outpatients and 72.0% inpatients who were prescribed antibiotics.

    CONCLUSIONS: Overall, treatment and medications prescribed adhered to current guidelines. There was a tendency to solely prescribe antibiotics in 8.6% of outpatients which is contrary to recommendations.

    Matched MeSH terms: Anti-Bacterial Agents*
  4. Ismail CAM, Deris ZZ, Bakar RA, Ismail N
    PMID: 33802184 DOI: 10.3390/ijerph18062834
    Despite modern medicine, there is an increasing trend for cases of the bacterial infection leptospirosis, and this has led to the exploration of alternative medicines from various sources including plants. The aim of this study was to investigate the in vitro anti-leptospiral activity of Phyllanthus amarus extracts alone and combined with penicillin G, ceftriaxone, and doxycycline. Antimicrobial susceptibility testing was performed using the microdilution broth technique upon methanol extract (ME), aqueous extract (AE), and antibiotics against the Leptospira interrogans serovars Australis, Bataviae, Canicola, and Javanica, to determine minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). The results were analyzed using an ELISA microplate reader combined with microscopic analysis. Synergy testing using a checkerboard assay was performed to determine the fractional inhibitory concentration index values of extracts combined with antibiotics against leptospires. Scanning electron microscopy (SEM) was used to investigate morphological changes of leptospires caused by potential anti-leptospiral agents alone and combined with antibiotics. The MICs and MBCs for P. amarus extracts ranged from 100 to 400 µg/mL for AEs and from 400 to 800 µg/mL for MEs. Penicillin G was the most effective anti-leptospiral drug, with MICs and MBCs ranging from <0.01 to 0.78 and <0.01 to 3.13 µg/mL, respectively, followed by ceftriaxone, with both MICs and MBCs ranging from 0.05 to 0.78 µg/mL, and doxycycline, with MICs and MBCs ranging from 0.39 to 3.13 µg/mL and 12.5 to 25 µg/mL, respectively. Combinations of P. amarus extracts and antibiotics did not show synergistic effects on all tested Leptospira serovars, with some combinations demonstrating antagonistic effects. SEM analysis, however, showed distorted Leptospira surfaces. P. amarus AE performed better anti-leptospiral activity than P. amarus ME. The morphological effects of P. amarus extract alone and its combination with antibiotic on Leptospira cells revealed promising anti-leptospiral properties.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology
  5. Kandasamy G, Sivanandy P, Almaghaslah D, Khobrani M, Chinnadhurai M, Vasudevan R, et al.
    Int J Clin Pract, 2020 Nov;74(11):e13599.
    PMID: 32593188 DOI: 10.1111/ijcp.13599
    BACKGROUND: Resistance to antibiotics causes negative impact on health of the patients. Antibiotic resistance is the major global concern that has to be nullified for the better health outcome. The knowledge on antibiotics is very essential for the students who undertake pharmacy and health science courses. Hence a study was aimed to assess the level of knowledge, attitude, perception and practice on antibiotics usage among the pharmacy students of King Khalid University, KSA.

    MATERIALS AND METHODS: A prospective online questionnaire based survey was carried out among the pharmacy students about knowledge, attitude perception and practice of antibiotics using 5-point "Likert scale" and true/false responses. The responses range from strongly agree to strongly disagree, and always to never were recorded. The data were analysed by using simple descriptive statistics.

    RESULTS: Out of 300 students approached 212 responded and the response rate was found to be 71%. In this study, most (95%) of the students were aware of the emerging problem of antibiotic resistance due to inappropriate use of antibiotics. Majority (89%) of the students agreed the inappropriate use of antibiotics can increase the overall cost of treatment. However, over half of the (54%) students were not aware of the antibiotic resistance that may be a nation-wide problem of Kingdom of Saudi Arabia. The net positive response (NPR) and net other response (NOR) of all items in perception on antibiotics received more or less equal responses.

    CONCLUSION: The knowledge of antibiotics usage among the students are considerably good compared with previous studies. However, the attitude, perception and practice on antibiotics usage among the pharmacy students are very poor. It alarms the need of appropriate education to enlighten antibiotic awareness for the better disease prevention and health outcomes for the benefit of patient community.

    Matched MeSH terms: Anti-Bacterial Agents/therapeutic use
  6. Ma C, Lo PK, Xu J, Li M, Jiang Z, Li G, et al.
    Bioresour Technol, 2020 Oct;314:123731.
    PMID: 32615447 DOI: 10.1016/j.biortech.2020.123731
    In this study, the differences on the physico-chemical parameters, lignocellulose degradation, dynamic succession of microbial community, gene expression of carbohydrate-active enzymes and antibiotics resistance genes were compared during composting systems of bagasse pith/pig manure (BP) and manioc waste/pig manure (MW). The results revealed that biodegradation rates of organic matter, cellulose, hemicellulose and lignin (29.14%, 17.53%,45.36% and 36.48%) in BP were higher than those (15.59%, 16.74%, 41.23% and 29.77%) in MW. In addition, the relative abundance of Bacillus, Luteimonas, Clostridium, Pseudomonas, Streptomyces and expression of genes encoding carbohydrate- active enzymes in BP were higher than those in MW based on metagenomics sequencing. During composting, antibiotics and antibiotic resistance genes were substantially reduced, but the removal efficiency was divergent in the both samples. Taken together, metagenomics analysis was a potential method for evaluating lignocellulose's biodegradation process and determining the elimination of antibiotic and antibiotic resistance genes from different composting sources of biomass.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology
  7. Yazid AM, Ali AM, Shuhaimi M, Kalaivaani V, Rokiah MY, Reezal A
    Lett Appl Microbiol, 2000 Jul;31(1):57-62.
    PMID: 10886616
    Eighteen Bifidobacterium strains were tested for their susceptibility to a range of antimicrobial agents. All the strains tested, including the reference culture Lactobacillus acidophilus CH2, were susceptible to several groups of antimicrobial agents, they were cephalosporin (cefamandole, cefazolin, cefaperazone, cefoxitin), polypeptide (bacitracin), macrolide (erythromycin), penicillin (amoxicillin), phenicol (chloramphenicol) and beta-lactam (imipenem). Fourteen strains were resistant to more than 10 antibiotics. The reference culture was resistant to only three antibiotics. The results showed that bifidobacteria are resistant to a wide range of antimicrobial agents.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology*
  8. Muthiah B, Muthukrishnan L, Anita Lett J, Sagadevan S, Kesavan S, Vennila S, et al.
    J Nanosci Nanotechnol, 2020 10 01;20(10):6326-6333.
    PMID: 32384982 DOI: 10.1166/jnn.2020.17897
    Biosynthesis of nanoparticles has now become a novel trend in addressing some of the environmental issues by adopting eco-friendly approaches in manoeuvring nanoparticles for various applications. Plants and micro-organisms have been the potential sources of the biological mode of synthesizing nanoparticles as part of their bioremediation process. This principle has been harnessed for synthesizing nanoparticles either extra or intracellularly. In this line of phyto-mediated synthesis, eucalyptus buds have been used for synthesizing gold nanoparticles (Au NPs) under optimized laboratory conditions. The UV-visible spectrum of the Au NPs showed typical surface plasmon resonance at 550 nm (λmax) with a crystalline phase measuring <100 nm in size and monodispersed as revealed from XRD, FESEM, and AFM analyses. The biological role of phytochemical concoction in reducing and stabilizing the Au NPs was clearly identified from FT-IR studies. The antimicrobial effect of the Au NPs against clinically important pathogens viz. Staphylococcus sp., Pseudomonas sp., Bacillus sp. and E. coli determined using the disk diffusion method showed no significant antibacterial effect at all concentrations. Cytotoxicity studies were carried using Vero and HEp-2 cell lines and the 50% inhibition concentration (IC50) was determined to be 1.25 mg and 0.625 mg/mL respectively. Au NPs with potential antimicrobial and anti-proliferative effects could found profound implications in the field of nanomedicine once the toxicity in vivo has been investigated.
    Matched MeSH terms: Anti-Bacterial Agents/toxicity
  9. Qing S, Shoutian Q, Hongyan G, Ming Y, Swamy MK, Sinniah UR, et al.
    J Nanosci Nanotechnol, 2019 07 01;19(7):4109-4115.
    PMID: 30764978 DOI: 10.1166/jnn.2019.16282
    This study reports the biosynthesis of silver nanoparticles (AgNPs) using methanolic leaf extract of Pogostemon cablin Benth. (Patchouli) as a reducing agent, and their potent biological (antibacterial, antioxidant and anticancer) activities. The P. cablin extract when exposed to silver nitrate reduced silver ions to form crystalline AgNPs within 1 h of incubation at room-temperature. UV-visible spectra showed a sharp surface plasmon resonance (SPR) at around 430 nm for the biosynthesized AgNPs and the XRD pattern indicated the crystalline planes of the face centered cubic silver. The FE-SEM analysis revealed the occurrence of predominant spherical shaped AgNPs with a huge disparity in their particle size distribution with an average size of 25 nm, while, the FTIR data confirmed the bio-reduction and capping of AgNPs by several phytocompounds present in the methanolic leaf extract. AgNPs effectively inhibited the growth of all the tested human pathogenic bacterial strains (Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli), while, the methanolic leaf extract failed to inhibit the growth of S. aureus and P. aeruginosa. AgNPs showed the highest free radical scavenging activity (79.0 ± 0.76%) compared to methanolic leaf extract (68.3 ± 0.68%) at 100 μg/ml. Further, the cytotoxicity study using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) confirmed that AgNPs successfully inhibited the human colon adenocarcinoma cell line (HT-29) in a dose dependent manner. At higher concentrations (500 μg/ml), only 4% of cells survived after 72 hrs of exposure with IC50 value of 120 μg/ml. Thus, these findings offer a new source of biomolecules with diverse biological activities.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology
  10. Venkatraman SK, Choudhary R, Krishnamurithy G, Raghavendran HRB, Murali MR, Kamarul T, et al.
    Mater Sci Eng C Mater Biol Appl, 2021 Jan;118:111466.
    PMID: 33255048 DOI: 10.1016/j.msec.2020.111466
    This work is aimed to develop a biocompatible, bactericidal and mechanically stable biomaterial to overcome the challenges associated with calcium phosphate bioceramics. The influence of chemical composition on synthesis temperature, bioactivity, antibacterial activity and mechanical stability of least explored calcium silicate bioceramics was studied. The current study also investigates the biomedical applications of rankinite (Ca3Si2O7) for the first time. Sol-gel combustion method was employed for their preparation using citric acid as a fuel. Differential thermal analysis indicated that the crystallization of larnite and rankinite occurred at 795 °C and 1000 °C respectively. The transformation of secondary phases into the desired product was confirmed by XRD and FT-IR. TEM micrographs showed the particle size of larnite in the range of 100-200 nm. The surface of the samples was entirely covered by the dominant apatite phase within one week of immersion. Moreover, the compressive strength of larnite and rankinite was found to be 143 MPa and 233 MPa even after 28 days of soaking in SBF. Both samples prevented the growth of clinical pathogens at a concentration of 2 mg/mL. Larnite and rankinite supported the adhesion, proliferation and osteogenic differentiation of hBMSCs. The variation in chemical composition was found to influence the properties of larnite and rankinite. The results observed in this work signify that these materials not only exhibit faster biomineralization ability, excellent cytocompatibility but also enhanced mechanical stability and antibacterial properties.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology
  11. Saidin S, Jumat MA, Mohd Amin NAA, Saleh Al-Hammadi AS
    Mater Sci Eng C Mater Biol Appl, 2021 Jan;118:111382.
    PMID: 33254989 DOI: 10.1016/j.msec.2020.111382
    In spite of antibiotics, antibacterial agents or specifically known as antiseptics are actively explored for the prevention of infection-associated medical devices. Antibacterial agents are introduced to overcome the complication of bacterial resistance which devoted by antibiotics. It can be classified into inorganic and organic, that prominently have impacted bacterial retardation in their own killing mechanism patterns. Therefore, this review paper aimed to provide information on most common used inorganic and organic antibacterial agents which have potential to be utilized in biomedical applications, thus, classifying the trends of antibacterial mechanism on Gram-negative and Gram-positive bacteria. In the beginning, infectious diseases and associated biomedical infections were stated to expose current infection scenarios on medical devices. The general view, application, susceptible bacteria and activation mechanism of inorganic (silver, copper, gold and zinc) and organic (chlorhexidine, triclosan, polyaniline and polyethylenimine) antibacterial agents that are widely proposed for biomedical area, were then gathered and reviewed. In the latter part of the study, the intact mechanisms of inorganic and organic antibacterial agents in retarding bacterial growth were classified and summarized based on its susceptibility on Gram-negative and Gram-positive bacteria. Most of inorganic antibacterial agents are in the form of metal, which release its ions to retard prominently Gram-negative bacteria. While organic antibacterial agents are susceptible to Gram-positive bacteria through organelle modification and disturbance of bio-chemical pathway. However, the antibacterial effects of each antibacterial agent are also depending on its effective mechanism and the species of bacterial strain. These compilation reviews and classification mechanisms are beneficial to assist the selection of antibacterial agents to be incorporated on/within biomaterials, based on its susceptible bacteria. Besides, the combination of several antibacterial agents with different susceptibilities will cover a wide range of antibacterial spectrum.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology
  12. Lee CL, Ng HF, Ngeow YF, Thaw Z
    J Med Microbiol, 2021 Jul;70(7).
    PMID: 34236301 DOI: 10.1099/jmm.0.001378
    Introduction. Tigecycline is currently acknowledged to be one of the most effective antibiotics against infections caused by Mycobacteroides abscessus.Gap statement. The genetic determinants of tigecycline resistance in M. abscessus are not well understood.Aim. In this study, we characterized a tigecycline-resistant M. abscessus mutant, designated CL7, to identify the potential resistance mechanism.Methodology. CL7 was characterized using antimicrobial susceptibility testing, whole-genome sequencing, PCR and RT-qPCR. For biological verification, gene overexpression assays were carried out.Results. Whole-genome sequencing and the subsequent gene overexpression assays showed that CL7 harboured a stop-gain mutation in MAB_3543 c, which may be responsible for the tigecycline resistance phenotype. This gene encodes an orthologue of SigH, which is involved in the positive regulation of physiological stress response and is negatively regulated by the RshA anti-sigma factor in Mycobacterium tuberculosis. We hypothesized that the MAB_3543 c mutation may disrupt the interaction between SigH and RshA (MAB_3542 c). RT-qPCR analyses revealed the upregulation of MAB_3543 c and other key stress response genes, which has previously been shown to be a hallmark of SigH-RshA bond disruption and tigecycline resistance.Conclusion. The MAB_3543c mutation may represent a novel determinant of tigecycline resistance in M. abscessus. The findings of this study will hopefully contribute to our knowledge of potential tigecycline resistance mechanisms in M. abscessus, which may lead to better diagnostics and treatment modalities in the future.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology*
  13. Podin Y, Sarovich DS, Price EP, Kaestli M, Mayo M, Hii K, et al.
    Antimicrob Agents Chemother, 2014;58(1):162-6.
    PMID: 24145517 DOI: 10.1128/AAC.01842-13
    Melioidosis is a potentially fatal disease caused by the saprophytic bacterium Burkholderia pseudomallei. Resistance to gentamicin is generally a hallmark of B. pseudomallei, and gentamicin is a selective agent in media used for diagnosis of melioidosis. In this study, we determined the prevalence and mechanism of gentamicin susceptibility found in B. pseudomallei isolates from Sarawak, Malaysian Borneo. We performed multilocus sequence typing and antibiotic susceptibility testing on 44 B. pseudomallei clinical isolates from melioidosis patients in Sarawak district hospitals. Whole-genome sequencing was used to identify the mechanism of gentamicin susceptibility. A novel allelic-specific PCR was designed to differentiate gentamicin-sensitive isolates from wild-type B. pseudomallei. A reversion assay was performed to confirm the involvement of this mechanism in gentamicin susceptibility. A substantial proportion (86%) of B. pseudomallei clinical isolates in Sarawak, Malaysian Borneo, were found to be susceptible to the aminoglycoside gentamicin, a rare occurrence in other regions where B. pseudomallei is endemic. Gentamicin sensitivity was restricted to genetically related strains belonging to sequence type 881 or its single-locus variant, sequence type 997. Whole-genome sequencing identified a novel nonsynonymous mutation within amrB, encoding an essential component of the AmrAB-OprA multidrug efflux pump. We confirmed the role of this mutation in conferring aminoglycoside and macrolide sensitivity by reversion of this mutation to the wild-type sequence. Our study demonstrates that alternative B. pseudomallei selective media without gentamicin are needed for accurate melioidosis laboratory diagnosis in Sarawak. This finding may also have implications for environmental sampling of other locations to test for B. pseudomallei endemicity.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology*
  14. Santhana Raj L, Hing HL, Baharudin O, Teh Hamidah Z, Aida Suhana R, Nor Asiha CP, et al.
    Trop Biomed, 2007 Jun;24(1):105-9.
    PMID: 17568383 MyJurnal
    Mesosomes of Staphylococcus aureus ATCC 25923 treated with antibiotics were examined morphologically under the electron microscope. The Transmission Electron Microscope Rapid Method was used to eliminate the artifacts due to sample processing. Mesosomes were seen in all the antibiotic treated bacteria and not in the control group. The main factor that contributes to the formation of mesosomes in the bacteria was the mode of action of the antibiotics. The continuous cytoplasmic membrane with infolding (mesosomes) as in the S. aureus ATCC 25923 is therefore confirmed as a definite pattern of membrane organization in gram positive bacteria assaulted by amikacin, gentamicin, ciprofloxacin, vancomycin and oxacillin antibiotics. Our preliminary results show oxacillin and vancomycin treated bacteria seemed to have deeper and more mesosomes than those treated with amikacin, gentamicin and ciprofloxacin. Further research is needed to ascertain whether the deep invagination and the number of mesosomes formed is associated with the types of antibiotic used.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology*
  15. Raju SV, Sarkar P, Pasupuleti M, Saraswathi NT, Arasu MV, Al-Dhabi NA, et al.
    PMID: 33465517 DOI: 10.1016/j.cbpc.2021.108974
    Development of antimicrobial drugs against multidrug-resistant (MDR) bacteria is a great focus in recent years. TG12, a short peptide molecule used in this study was screened from tachykinin (Tac) protein of an established teleost Channa striatus (Cs) transcriptome. Tachykinin cDNA has 345 coding sequence, that denotes a protein contained 115 amino acids; in which a short peptide (TG12) was identified at 83-94. Tachykinin mRNA upregulated in C. striatus treated with Aeromonas hydrophila and Escherichia coli lipopolysaccharide (LPS). The mRNA up-regulation was studied using real-time PCR. The up-regulation tachykinin mRNA pattern confirmed the immune involvement of tachykinin in C. striatus during infection. Further, the identified peptide, TG12 was synthesized and its toxicity was demonstrated in hemolytic and cytotoxic assays using human erythrocytes and human dermal fibroblast cells, respectively. The toxicity study exhibited that the toxicity of TG12 was similar to negative control, phosphate buffer saline (PBS). Moreover, the antibiogram of TG12 was active against Klebsiella pneumonia ATCC 27736, a major MDR bacterial pathogen. Further, the antimicrobial activity of TG12 against pathogenic bacteria was screened using minimum inhibitory concentration (MIC) and anti-biofilm assays, altogether TG12 showed potential activity against K. pneumonia. Fluorescence assisted cell sorter flow cytometer analysis (FACS) and field emission scanning electron microscopy (FESEM) was carried on TG12 with K. pneumonia; the results showed that TG12 significantly reduced K. pneumonia viability as well as TG12 disrupt its membrane. In conclusion, TG12 of CsTac is potentially involved in the antibacterial immune mechanisms, which has a prospectus efficiency in pharma industry against MDR strains, especially K. pneumonia.
    Matched MeSH terms: Anti-Bacterial Agents/pharmacology*
  16. Koh CT, Ghazi HF, Ahmad MI, Abdul Samad N, Lai KEY, Ismail NF, et al.
    Health Serv Res Manag Epidemiol, 2016 May 5;3:2333392816643720.
    PMID: 28462275 DOI: 10.1177/2333392816643720
    OBJECTIVES: To assess the knowledge, attitude, and practice of parents regarding antibiotic usage for treating upper respiratory tract infection (URTI) among children.
    METHODS: A cross-sectional study was conducted among 320 randomly selected parents attending a primary health clinic using self-administered questionnaires.
    RESULTS: About two-thirds (69.1%) of the parents had poor knowledge level. Only 25.2% and 21.6% of the parents could correctly identify amoxicillin and penicillin as the treatment of children's URTI. However, about two-thirds (67.5%) of the parents were aware of the antibiotic resistance caused by overuse of antibiotics. A significant association was noted between the father's and mother's educational level and family income with the knowledge level. Only mother's educational level depicted a significant association with the attitude.
    CONCLUSION: The knowledge of parents regarding antibiotic usage for URTI was poor. More numbers of health promotions and educational campaigns are required to help parents understand about antibiotic usage.
    Study site: Primary care clinic, Bandar Tasik Selatan, Kuala Lumpur, Malaysia
    Matched MeSH terms: Anti-Bacterial Agents*
  17. Saarani NN, Jamuna-Thevi K, Shahab N, Hermawan H, Saidin S
    Dent Mater J, 2017 May 31;36(3):260-265.
    PMID: 28111388 DOI: 10.4012/dmj.2016-177
    A guided bone regeneration (GBR) membrane has been extensively used in the repair and regeneration of damaged periodontal tissues. One of the main challenges of GBR restoration is bacterial colonization on the membrane, constitutes to premature membrane degradation. Therefore, the purpose of this study was to investigate the antibacterial efficacy of triple-layered GBR membrane composed of poly(lactic-co-glycolic acid) (PLGA), nanoapatite (NAp) and lauric acid (LA) with two types of Gram-negative periodontal bacteria, Fusobacterium nucleatum and Porphyromonas gingivalis through a disc diffusion and bacterial count tests. The membranes exhibited a pattern of growth inhibition and killing effect against both bacteria. The increase in LA concentration tended to increase the bactericidal activities which indicated by higher diameter of inhibition zone and higher antibacterial percentage. It is shown that the incorporation of LA into the GBR membrane has retarded the growth and proliferation of Gram-negative periodontal bacteria for the treatment of periodontal disease.
    Matched MeSH terms: Anti-Bacterial Agents*
  18. Hussein bin Mohamed Sal
    Med J Malaysia, 1973 Jun;27(4):262-70.
    PMID: 4270783
    Matched MeSH terms: Anti-Bacterial Agents/therapeutic use
  19. Ponnampalam JT
    Med J Malaya, 1965 Mar;19(3):222-3.
    PMID: 4220474
    Matched MeSH terms: Anti-Bacterial Agents/therapeutic use
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