In this study polymerase chain reaction (PCR) was used to identify yeast in domestic ragi obtained
from two local markets in Sarawak and Pahang. These ragi are normally used as a dry starter in food fermentation (tapai) for Pahang (ST2) and Sarawak (ST3) and tuak (ST1) which is an alcoholic drink in Sarawak. Universal primer, NL1 and NL4 were used as a primer in this study to amplify D1/D2 fragment. Based on the result from the sequencing and after the BLAST search of the nucleotide sequences, the strain was confirmed as Candida glabrata (FN424108.) partial 26S rRNA gene, strain IMUFRJ 51955 for ST1, Saccharomyces cerevisiae(EU285514.1) isolate 35 26S ribosomal RNA gene, partial sequence for ST2 sample and Candida glabrata (FN393990.1) partial 26S rRNA gene, strain MUCL 51244 for ST3. All these strains were found in domestic ragi used for food fermentation.
Blends of poly (vinyl chloride) and poly (ethylene oxide) with the addition of carbon black
as filler was prepared via solution casting technique to fabricate a conductive polymer film. Besides,
poly (ethylene glycol diglycidyl ether) (PEGDE) was added into the formulation in order to improve its
properties. The surface morphology of the conductive polymer film was characterized via scanning
electron microscope (SEM). The results showed that the conductivity of the film was greatly enhanced
by incorporating the PEGDE as the surface modifier in the PVC/PEO conductive polymer film.