The main objective of this study is to determine the association between respirable hexavalent chromium compounds with urinary β2-microglobulin levels among welders in an automotive components manufacturing plant. 49 welders and 39 workers involved in stamping process were selected as the exposed and the comparative group. β2-microglobulin is a protein renal tubular dysfunction marker that can indicate renal dysfunction caused by heavy metal. Air samples of worker’s breathing zone were collected using personal air sampling pump and filter papers. Filter papers were then diluted and analysed with Atomic Absorption Spectrophotometry (AAS). Workers’ urine samples were collected at the end of 8-hour work shift and analysed with β2-microglobulin ELISA Kit (IBL-Hamburg) and a microtiter reader. Meanwhile, creatinine levels were analysed with creatinine test strips and Reflotron®. A mean concentration of respirable hexavalent chromium compounds in air for the exposed group was 0.135 ± 0.043μg/m3 while for the non-exposed group was 0.124 ± 0.029μg/m3. The mean level of urinary β2-microglobulin per creatinine for the exposed group was 84.996 ± 39.246μg/g while that of the comparative group was 61.365 ± 21.609μg/g. The concentrations of respirable hexavalent chromium compounds were higher in the exposed group compared to the comparative group (Z=-2.444, p=0.015). β2-microglobulin level was also higher in the exposed group compared to the non-exposed group (t=3.821, p=
Homocysteine could be a mechanism that underlies the effects of lead on cardiovascular system. This study aims to identify the relationship between lead exposure and homocysteine levels among workers. A comparative cross-sectional study was carried out on 80 workers of an automotive components manufacturing factory; that comprised of 40 exposed workers and 40 non-exposed workers. Blood samples of respondents were taken by fingerprick. The blood samples were analyzed for blood lead concentration by using Atomic Absorption Spectrometry Graphite Furnace Model GBC 908AA. Besides that, ELISA Kit was used to show the homocysteine level among the respondents. Questionnaires were used to obtain demography information of respondents. Results from the statistical analysis showed that the mean blood lead concentration for exposed respondents was 5.53±4.74 μg/dL and 3.53±2.81 μg/dL for the comparative respondents. Mann-Whitney U test showed that there was no significance difference between the mean blood lead concentration of the exposed and comparative group (z=-1.178; p=0.075). The blood lead concentration ranged 0.68-17.95 among the exposed group and with a range of 0.084-11.96 for the comparative group. The mean homocysteine level (μmol/L) was 32.48±2.481μmol/L for the exposed group and 16.50±4.0960 μmol/L for the comparative group. There was a significant difference in homocysteine level (μmol/L) between the exposed (32.48±2.481) and comparative (16.50±4.0959) groups (z = -7.699, p
The objective of this study was t0 determine the relationship between scores on McCarthy Scales of Children's Abilities (MSCA) and academic peU’ormance of school children. A total ofI12first and second graders aged 6 to 8 I/2 years old hom two primary schools in Kuala Lumpur, Malaysia were selected as subjects for this study. This selection was based on a random sampling stratyied proportionalhr according to school and sex. The MSCA was administered to 59 male and 53 female students. The test battery was translated from English to Malay and adapted to suit the local situation. Academic performance was measured using the mean percent of the total score on all the subjects (English, Malay, Mathematics and Islamic Religion) in the jinal examinations at the end of the year. T—test results indicated no signdicant dfference between MSCA scores and academic scores of maleandkmale students. The results revealed that all the scores on the MSCA and the General Cognitive Index were signyicantbr correlated with academic pejormance, The General Cognitive Index accounted for [0.4% ofthe variation in academic performance. T he findings appear to support that intelligence scores may be used to predict children 's academic performance in the early years of primary education.
With economic growth and significant technological advances in the health sector, many countries have developed aggregate outcomes in terms of both health services and individual well-being. Life expectancy has seen a remarkable increase of more than fifty per cent between 1950 and 2009. Achievement is uneven, however, and some groups are better able to access health services than others. In our review, we explore the need and how to maximize health equity, efficiency and effectiveness. Methodology is the review and web surfing on public health, social science, humanity and development literature. The increasing gap in health inequality, however, calls for further reform of the health system to achieve both equity and efficiency. Health is essential for survival and human capability. Good health enables people to participate in society. A new approach to efficient and cost-effective health service provision is community participation in health development. Participation can increase the skills and knowledge of local people, thus providing opportunities to improve their lives (empowerment). Analysis suggests four functional changes to achieve equity and efficiency in maximizing health outputs: reforms targeting universal coverage to achieve universal access to health; people-centred service delivery through concentrating on health services based on need; public policy change targeting integrated and multi-system health planning; and collective health system and community response to achieve health for all.
Culture expanded chondrocytes isolated from non-load bearing region of osteoarthritic (OA) joint has been used to construct tissue engineered cartilage for treatment purposes. The aim of the study was to compare the histological properties of the cartilage tissue and morphological properties of the chondrocytes isolated from less and severely affected OA knee. Human articular cartilage was obtained as redundant tissue from consented patients with late-stage OA undergoing total knee replacement surgery at Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Articular cartilage was graded according to Dougados and Osteoarthritis Research Society International (OARSI) classification. Articular cartilage was classified into less affected (LA; Grade 0-1) and severely affected (SA; Grade 2-3). Cartilage tissue from less and severely affected region was stained with Safranin O staining. Isolated chondrocytes from each group were cultured until passage 4 (P4). Their growth patterns, cell areas, and circularity were compared. LA-cartilage tissue shows uniform spread of safranin O staining indicating intact extracellular matrix (ECM) component. However, SA-cartilage shows significant reduction and unstable staining due to its degraded ECM. LA-chondrocytes showed an aggregated growth compared to SA-chondrocyte that remains monolayer. Moreover, LA-chondrocytes have significantly higher cell area with wider spreading at passage 0 and 4 compared to SA-chondrocytes. It was also found that chondrocyte circularity increased with passage, and circularity of LAchondrocytes was significantly higher than that of the SA-chondrocytes at passage 3. This study demonstrated the considerable difference in the cellular properties for less and severely affected chondrocytes and implication of these differences in cell-based therapy needed to be explored.