The skin can be vulnerable to various microbial infection. Although antibiotics are clinically proven to be useful in the treatment of bacterial skin infections, they are largely subjected to antibiotic resistance and adverse effects. This has led to the screening of several medicinal plants for their potential antimicrobial activity since they are less expensive, has reduced occurrence of adverse effects and widespread availability. The aim of this research will focus on evaluating the antibacterial activity of different extracts of Ipomoea aquatica leaves against Staphylococcus aureus and Streptococcus pyogenes that causes skin infections. Leaves were extracted separately with 95% methanol and 95% ethanol using maceration process. Phytochemical screening was done for each extract and the minimum inhibitory concentration (MIC) was determined for each extract against both bacteria using 10 different concentrations ranging from 10mg/ml up to 100mg/ml via disc diffusion method in triplicates. Two concentrations above the MIC from each extract were selected and antibacterial assay of the different extracts against the two bacteria respectively was performed using disc diffusion method in triplicates. MIC for methanolic extract against both bacteria was 10mg/ml, while MIC for ethanolic extract was 10mg/ml against Staphylococcus aureus and 30mg/ml against Streptococcus pyogenes. Methanolic extract of the plant at a concentration of 90mg/ml and 100mg/ml was statistically significant against Streptococcus pyogenes with a significance value of 0.00 (p
Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M) as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop sustained released matrix tablets. The formulated matrix tablets were evaluated in terms of physical appearance, weight variation, thickness, diameter, hardness, friability and in vitro drug release. The difference between the natural and synthetic polymers was investigated concurrently. Matrix tablets developed from each formulation passed all standard physical evaluation tests. The dissolution studies of formulated tablets revealed sustained drug release up to 24 h compared to the reference drug Apo Keto® SR tablets. The dissolution data later were fitted into kinetic models such as zero order equation, first order equation, Higuchi equation, Hixson Crowell equation and Korsmeyer-Peppas equation to study the release of drugs from each formulation. The best formulations were selected based on the similarity factor (f2) value of 50% and more. Through the research, it is found that by increasing the polymers concentration, the rate of drug release decreased for both natural and synthetic polymers. The best formulation was found to be F3 which contained 40% Hibiscus rosa-sinensis mucilage polymer and showed comparable dissolution profile to the reference drug with f2 value of 78.03%. The release kinetics of this formulation has shown to follow non-Fickian type which involved both diffusion and erosion mechanism. Additionally, the statistical results indicated that there was no significant difference (p > 0.05) between the F3 and reference drug in terms of MDT and T50% with p-values of 1.00 and 0.995 respectively.