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Fall risk prevention: The related factors of nurses practice at general local hospital in Indonesia

Suryani L, Perdani AL, Dioso RI, Hoon LS

Enferm Clin, 2020 06;30 Suppl 5:221-223.
PMID: 32713575 DOI: 10.1016/j.enfcli.2019.11.059

OBJECTIVE: This study aimed was to describe related factors of nurse practice in preventing fall risk in an inpatient ward at X General Local Hospital.
METHOD: A descriptive, analytical quantitative with a cross-sectional approach was used in this study. The total of 95 nurses agreed to participate by using random sampling. Data collection using a structured questionnaire and observational form. The statistical model with a chi-square analysis was used in this study.
RESULTS: The result showed a correlation between knowledge and nurse practice with OR 3.257 (1.375-7.715; p=0.012), attitude and nurse practice with OR 4.286 (1.775-10.345; p=0.002) training and nurse practicewith OR value 5.455 (2.233-13.322; p=0.000).
CONCLUSIONS: Local authority in the hospital must apply patient safety standards to reduce injury rates, both nurses and patients. Nurses need to follow the current trend of nursing science focusing on patient safety.
Suppressive effect of maslinic acid on PMA-induced protein kinase C in human B-lymphoblastoid cells

Mooi LY, Yew WT, Hsum YW, Soo KK, Hoon LS, Chieng YC

Asian Pac J Cancer Prev, 2012;13(4):1177-82.
PMID: 22799301

Protein kinase C (PKC) has been implicated in carcinogenesis and displays variable expression profiles during cancer progression. Studies of dietary phytochemicals on cancer signalling pathway regulation have been conducted to search for potent signalling regulatory agents. The present study was designed to evaluate any suppressive effect of maslinic acid on PKC expression in human B-lymphoblastoid cells (Raji cells), and to identify the PKC isoforms expressed. Effects of maslinic acid on PKC activity were determined using a PepTag assay for non-radioactive detection of PKC. The highest expression in Raji cells was obtained at 20 nM PMA induced for 6 hours. Suppressive effects of maslinic acid were compared with those of four PKC inhibitors (H- 7, rottlerin, sphingosine, staurosporine) and two triterpenes (oleanolic acid and ursolic acid). The IC₅₀ values achieved for maslinic acid, staurosporine, H-7, sphingosine, rottlerin, ursolic acid and oleanolic acid were 11.52, 0.011, 0.767, 2.45, 5.46, 27.93 and 39.29 μM, respectively. Four PKC isoforms, PKC βI, βII, δ, and ζ, were identified in Raji cells via western blotting. Maslinic acid suppressed the expression of PKC βI, δ, and ζ in a concentration-dependent manner. These preliminary results suggest promising suppressive effects of maslinic acid on PKC activity in Raji cells. Maslinic acid could be a potent cancer chemopreventive agent that may be involved in regulating many downstream signalling pathways that are activated through PKC receptors.
Influence of baby massage on sleep quantity in baby of 3-6 months age in Tirawuta health center, East Kolaka District, Indonesia

Wahyuni S, Lestari SA, Zoahira WOA, Masriwatii S, Hoon LS

Enferm Clin, 2020 06;30 Suppl 5:224-227.
PMID: 32713576 DOI: 10.1016/j.enfcli.2019.11.060

Based on the results of the initial survey it was known that the number of babies who visited Puskesmas Tirawuta in 2017 were 42 in number. Whereas in 2018 March-May there were 25 babies at Puskesmas Tirawuta, East Kolaka District. In addition, the initial interview on March 26, 2018 showed that the author did this at Puskesmas Tirawuta, and it obtained a mother who had a baby and visited for immunizations. About 7 people said the baby had trouble in sleeping and sometimes they were fussy. Besides, some report frequent waking up when sleeping at night, crying when waking up and so on. Among the mothers who brought their babies to Puskesmas, 7 mothers said they knew or had heard about baby massage but is not knowledgeable how to massage properly. About 3 people said that at the same time they did not know how to massage a baby. The purpose of this study was to determine the effect of baby massage toward the quantity of sleeping among the babies aged 3-6 month at the Puskesmas Tirawuta in East Kolaka District. The design of this study is quasi-experimental where the design used was "One Group Pretest-Posttest Design". The population in this study was the number of babies aged 3-6 months with decreased sleeping quantity. Nearly 25 babies with a sample of 13 respondents were there. Data was processed using Paired T-Test. The results of the analysis obtained the value of t count=3.742>1.356 where t table for df 12 with the results of statistical tests was significant at a value 0.003<0.20. This showed that there was a significant effect of giving baby massage on the quantity of sleep among babies aged 3-6 months at Puskesmas Tirawuta, East Kolaka District. From the results of the research, suggestions were proposed that health workers need to conduct counseling, demonstration and distribution of leaflets. Hence, mothers were motivated to massage their babies. This could be done through Integrated Healthcare Center activities.
Cancer chemopreventive activity of maslinic acid: suppression of COX-2 expression and inhibition of NF-κB and AP-1 activation in Raji cells

Hsum YW, Yew WT, Hong PL, Soo KK, Hoon LS, Chieng YC, et al.

Planta Med, 2011 Jan;77(2):152-7.
PMID: 20669087 DOI: 10.1055/s-0030-1250203

Chronic inflammation is one of the predisposing factors for neoplastic transformation. Targeting inflammation through suppression of the pro-inflammatory pathway by dietary phytochemicals provides an important strategy for cancer prevention. Maslinic acid is a novel natural triterpenoid known to inhibit proliferation and induce apoptosis in some tumor cell lines. Although maslinic acid has cytotoxic and pro-apoptotic effects on cancer cells, the underlying mechanisms of its effects on the inflammatory pathway have yet to be elucidated. It has been reported that abnormal expression of pro-inflammatory enzyme cyclooxygenase-2 (COX-2) causes promotion of cellular proliferation, suppression of apoptosis, enhancement of angiogenesis and invasiveness. In the present study, the suppressive effect of maslinic acid on COX-2 expression and the binding activity of upstream transcription factors NF- κB and AP-1, which are known to regulate COX-2 transcriptional activation, were assessed using Raji cells. The anti-inflammatory action of maslinic acid was benchmarked against oleanolic acid and other standard drugs. Western blot analysis and electrophoretic mobility shift assay (EMSA) were employed to analyze COX-2 expression as well as NF- κB and AP-1 binding activity. Our results showed that maslinic acid suppresses COX-2 expression in a concentration-dependent manner. Likewise, the constitutive nuclear NF- κB (p65) activity as well as phorbol 12-myristate 13-acetate (PMA)- and sodium N-butyrate (SnB)-induced AP-1 binding activity in Raji cells were significantly reduced following treatment with maslinic acid. Since maslinic acid suppresses COX-2 expression in Raji cells at concentrations that also lowered the NF- κB (p65) and AP-1 binding activity, it is possible that the suppression of COX-2 by this natural triterpenoid might be achieved, at least in part, via the NF- κB and AP-1 signaling pathways.