Synthesis of nanoparticles by using plant have sparked interest among researchers due to environmentally safe, inexpensive and simple method to compare with chemical method. Use of plant in synthesis zinc oxide nanoparticles (ZnO NPs) that act as reducing and capping agent are more recommended, due to high production of product and rate of synthesis is faster than using microorganism. This study focus on the synthesis of ZnO NPs by using leaf extract of aloe vera (Aloe bardenisis miller) with different concentration (30%, 40% and 50%) and various calcination temperature which are 500 ˚C, 700 ˚C and 900 ˚C for 4 hours. Fourier – transform infrared spectroscopy (FTIR), Thermogravimetric Analysis (TGA), scanning electron microscopy (SEM), X-ray Diffraction (XRD) and Brunauer-Emmet and Teller (BET) were used to characterize the prepared samples. FTIR spectra showed present wavenumber in between 400-500 cm-1 indicated the presence of Zn-O stretch. Powder XRD pattern confirmed the hexagonal wurtzite structure with average particles size from 24.19 nm to 67.69 nm for all concentration and temperature by using Scherer’s equation. For SEM analysis the images show irregular shape for concentrations 30% and 50% with size range from 500 nm to 900 nm while for concentration 40% cubic shape was observe with size range from 140 nm to 900 nm. All characterize show that formation of ZnO NPs depend on the concentration and calcination temperature. Sample 30% and 50% ZnO NPs was applied in lithium battery at voltage from 0.01 to 3. 1.2 mAhg-1 was recorded for sample 30% ZnO NPs while 100 mAhg-1.