A large amount of ammonia volatilization from the agricultural system causes environmental problems and increases production costs. Conservation agriculture has emerged as an alternate and sustainable crop production system. Therefore, in the present study, ammonia losses from different agricultural practices were evaluated for the wheat crop under different tillage practices. The results of the present study showed that the cumulative emission of ammonia flux from the wheat field varied from 6.23 to 24.00 kg ha-1 (P ≤ 0.05) in conservation tillage (CA) and 7.03 to 26.58 kg ha-1 (P ≤ 0.05) in conventional tillage (CT) among different treatments. Application of basal 80% nitrogen resulted in the highest ammonia flux in conventional and conservation tillage practices. The ammonia volatilization followed the following trend: urea super granules with band placement > neem-coated urea with band placement > neem-coated urea with broadcast before irrigation > neem-coated urea with broadcast after irrigation > slow-release N fertilizer (urea stabilized with DCD and N(n-butyl)thiophosphoric triamide) with band placement. The conservation agricultural practices involving conservation tillage appear to be a sustainable approach for minimizing ammonia volatilization and improving wheat productivity.