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Insight in the quorum sensing-driven lifestyle of the non-pathogenic Agrobacterium tumefaciens 6N2 and the interactions with the yeast Meyerozyma guilliermondii

Bertini EV 1 , Torres MA 1 , Léger T 2 , Garcia C 3 , Hong KW 4 , Chong TM 5 Show all authors , Castellanos de Figueroa LI 6 , Chan KG 7 , Dessaux Y 8 , Camadro JM 9 , Nieto-Peñalver CG 10

Affiliations 

  • 1 PROIMI, CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Av. Belgrano y Pje. Caseros, Tucumán, Argentina
  • 2 ProteoSeine@IJM, Université de Paris, CNRS, Institut Jacques Monod, F-75006 Paris, France; Fougères Laboratory, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 35306 Fougères CEDEX, France
  • 3 ProteoSeine@IJM, Université de Paris, CNRS, Institut Jacques Monod, F-75006 Paris, France
  • 4 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; International Genome Centre, Jiangsu University, 212013 Zhenjiang, China
  • 5 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Institute of Marine Sciences, Shantou University, Shantou 515063, China
  • 6 PROIMI, CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Av. Belgrano y Pje. Caseros, Tucumán, Argentina; Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina
  • 7 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; International Genome Centre, Jiangsu University, 212013 Zhenjiang, China; Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
  • 8 Institut de Biologie Intégrative de la Cellule, Université Paris-Sud, Université Paris-Saclay, CNRS, CEA, F-91190 Gif-sur-Yvette, France
  • 9 CNRS, Institut Jacques Monod, Univ. Paris Diderot, Paris, France
  • 10 PROIMI, CONICET (Planta Piloto de Procesos Industriales Microbiológicos), Av. Belgrano y Pje. Caseros, Tucumán, Argentina; Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina. Electronic address: [email protected]
Genomics, 2021 11;113(6):4352-4360.
PMID: 34793950 DOI: 10.1016/j.ygeno.2021.11.017

Abstract

Agrobacterium tumefaciens is considered a prominent phytopathogen, though most isolates are nonpathogenic. Agrobacteria can inhabit plant tissues interacting with other microorganisms. Yeasts are likewise part of these communities. We analyzed the quorum sensing (QS) systems of A. tumefaciens strain 6N2, and its relevance for the interaction with the yeast Meyerozyma guilliermondii, both sugarcane endophytes. We show that strain 6N2 is nonpathogenic, produces OHC8-HSL, OHC10-HSL, OC12-HSL and OHC12-HSL as QS signals, and possesses a complex QS architecture, with one truncated, two complete systems, and three additional QS-signal receptors. A proteomic approach showed differences in QS-regulated proteins between pure (64 proteins) and dual (33 proteins) cultures. Seven proteins were consistently regulated by quorum sensing in pure and dual cultures. M. guilliermondii proteins influenced by QS activity were also evaluated. Several up- and down- regulated proteins differed depending on the bacterial QS. These results show the QS regulation in the bacteria-yeast interactions.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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