Biorefinery of Chlorella sorokiniana using ultra sonication assisted liquid triphasic flotation system

Koyande AK 1 , Chew KW 2 , Lim JW 3 , Lam MK 4 , Ho YC 5 , Show PL 6

Affiliations 

  • 1 Department of Chemical Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Selangor Darul Ehsan, Malaysia
  • 2 School of Mathematical Sciences, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Selangor Darul Ehsan, Malaysia
  • 3 Department of Fundamental and Applied Sciences, HICoE-Centre for Biofuel and Biochemical Research, Institute of Self-Sustainable Building, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak Darul Ridzuan, Malaysia
  • 4 Department of Chemical Engineering, HICoE-Centre for Biofuel and Biochemical Research, Institute of Self-Sustainable Building, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak Darul Ridzuan, Malaysia
  • 5 Department of Civil and Environmental Engineering, Universiti Teknologi PETRONAS, 32610 Seri Iskandar, Perak, Malaysia
  • 6 Department of Chemical Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Selangor Darul Ehsan, Malaysia. Electronic address: [email protected]
Bioresour Technol, 2020 May;303:122931.
PMID: 32044648 DOI: 10.1016/j.biortech.2020.122931

Abstract

The aim of this work was to study the ultrasonication-assisted Liquid Tri-phasic Flotation (LTF) System to obtain lipid and protein from microalgae Chlorella sorokiniana in a single step as a novel process. In the current study, biorefinery of Chlorella sorokiniana was performed using LTF system in a single step. The highest protein recovery of 97.43 ± 1.67% and lipid recovery of 69.50 ± 0.54% were obtained. The corresponding parameters were microalgae biomass loading of 0.5 w/v%, ammonium sulphate concentration of 40 w/v%, volume ratio of 1:1.5 (salt:alcohol), ultrasonication pulse mode of 20 s ON/20 s OFF at 20% amplitude for 5 mins, flotation air flowrate of 100 mL/min. Additionally, recycling of alcohol phase to study the circular nature of proposed biorefinery was investigated. The proposed LTF system for extraction of proteins and lipid reduces the number of operation units required in this biorefinery approach.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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