Leucaena leucocephala leachate compromised membrane integrity, respiration and antioxidative defence of water hyacinth leaf tissues

BACKGROUND: Water hyacinth is an invasive aquatic weed in many regions of the world. In this study, the bioherbicidal potential of allelopathic plant Leucaena leucocephala against water hyacinth was investigated using a leaf disc assay.

RESULTS: L. leucocephala leachate enhanced electrolyte leakage from water hyacinth leaf discs in a concentration-dependent manner. Control experiments eliminated the possibilities that increased membrane permeability in the leachate-treated leaf discs was due to pH or osmotic effects of the leachate. Thus, the loss of membrane stability in the leachate-treated leaf discs was likely due to phytotoxins detected in the leachate, namely mimosine and phenolic constituents. Decline in tissue respiration was detected in leachate-treated water hyacinth leaf discs. This suggests that the L. leucocephala leachate may contain compounds which acted as respiratory inhibitors. Enhanced reactive oxygen species production coincided with inhibition of catalase and ascorbate peroxidase activities in the leachate-treated water hyacinth leaf tissues. The injurious effects of L. leucocephala leachate on water hyacinth leaf discs probably involved direct inhibition of antioxidant enzymes in addition to direct involvement of some allelochemicals in reactive oxygen species formation.

CONCLUSION: In summary, the toxic effects of L. leucocephala leachate on water hyacinth leaf discs likely lay in its ability to effectively compromise the membrane integrity, tissue respiration and antioxidant defence of the latter.